LOCUS       NZ_CP110895            13336 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter freundii strain CF1807 plasmid pCF1807-6, complete
            sequence.
ACCESSION   NZ_CP110895
VERSION     NZ_CP110895.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31693425
            Assembly: GCF_026240835.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 13336)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Citrobacter freundii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13336)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2022) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026240835.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:41:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,783
            CDSs (total)                      :: 5,661
            Genes (coding)                    :: 5,524
            CDSs (with protein)               :: 5,524
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 41 of 137
            Pseudo Genes (incomplete)         :: 93 of 137
            Pseudo Genes (internal stop)      :: 23 of 137
            Pseudo Genes (multiple problems)  :: 18 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110895.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13336
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="CF1807"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pCF1807-6"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2018-07-17"
                     /collected_by="zhangxiaofan"
     gene            complement(77..343)
                     /locus_tag="OQW59_RS28100"
                     /old_locus_tag="OQW59_28100"
     CDS             complement(77..343)
                     /locus_tag="OQW59_RS28100"
                     /old_locus_tag="OQW59_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(327..551)
                     /locus_tag="OQW59_RS28105"
                     /old_locus_tag="OQW59_28105"
     CDS             complement(327..551)
                     /locus_tag="OQW59_RS28105"
                     /old_locus_tag="OQW59_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1097..1285
                     /locus_tag="OQW59_RS28110"
                     /old_locus_tag="OQW59_28110"
     CDS             1097..1285
                     /locus_tag="OQW59_RS28110"
                     /old_locus_tag="OQW59_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1333..1533)
                     /locus_tag="OQW59_RS28115"
                     /old_locus_tag="OQW59_28115"
     CDS             complement(1333..1533)
                     /locus_tag="OQW59_RS28115"
                     /old_locus_tag="OQW59_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024148903.1"
                     /translation="MNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            2704..3135
                     /locus_tag="OQW59_RS28120"
                     /old_locus_tag="OQW59_28120"
     CDS             2704..3135
                     /locus_tag="OQW59_RS28120"
                     /old_locus_tag="OQW59_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            3227..3733
                     /locus_tag="OQW59_RS28125"
                     /old_locus_tag="OQW59_28125"
     CDS             3227..3733
                     /locus_tag="OQW59_RS28125"
                     /old_locus_tag="OQW59_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265970816.1"
                     /translation="MRKTLLLCVPLFLLAGCAQQKQQMPDANYEKFAKVEVASDACLKS
                     NFITAQEAGQAHSNISLFLSSWAYDYVRYSNLLAQGHEEVKKIKITQEGCNLLRAKIYQ
                     DNIEAQRYQKQMEMAAQQKALADQQALQSIQNMQNTLPKTTYCNQIGTQTICKGSVPAE
                     GEMTP"
                     /name="hypothetical protein"
     gene            complement(3730..4335)
                     /locus_tag="OQW59_RS28130"
                     /old_locus_tag="OQW59_28130"
     CDS             complement(3730..4335)
                     /locus_tag="OQW59_RS28130"
                     /old_locus_tag="OQW59_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_265969940.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDHSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            4430..7327
                     /locus_tag="OQW59_RS28135"
                     /old_locus_tag="OQW59_28135"
     CDS             4430..7327
                     /locus_tag="OQW59_RS28135"
                     /old_locus_tag="OQW59_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(7437..7703)
                     /locus_tag="OQW59_RS28140"
                     /old_locus_tag="OQW59_28140"
     CDS             complement(7437..7703)
                     /locus_tag="OQW59_RS28140"
                     /old_locus_tag="OQW59_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262326.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7687..7911)
                     /locus_tag="OQW59_RS28145"
                     /old_locus_tag="OQW59_28145"
     CDS             complement(7687..7911)
                     /locus_tag="OQW59_RS28145"
                     /old_locus_tag="OQW59_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            8457..8645
                     /locus_tag="OQW59_RS28150"
                     /old_locus_tag="OQW59_28150"
     CDS             8457..8645
                     /locus_tag="OQW59_RS28150"
                     /old_locus_tag="OQW59_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8693..8893)
                     /locus_tag="OQW59_RS28155"
                     /old_locus_tag="OQW59_28155"
     CDS             complement(8693..8893)
                     /locus_tag="OQW59_RS28155"
                     /old_locus_tag="OQW59_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024148903.1"
                     /translation="MNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRHIVSDTNQAR
                     RSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            10065..10496
                     /locus_tag="OQW59_RS28160"
                     /old_locus_tag="OQW59_28160"
     CDS             10065..10496
                     /locus_tag="OQW59_RS28160"
                     /old_locus_tag="OQW59_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            10588..11094
                     /locus_tag="OQW59_RS28165"
                     /old_locus_tag="OQW59_28165"
     CDS             10588..11094
                     /locus_tag="OQW59_RS28165"
                     /old_locus_tag="OQW59_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265970816.1"
                     /translation="MRKTLLLCVPLFLLAGCAQQKQQMPDANYEKFAKVEVASDACLKS
                     NFITAQEAGQAHSNISLFLSSWAYDYVRYSNLLAQGHEEVKKIKITQEGCNLLRAKIYQ
                     DNIEAQRYQKQMEMAAQQKALADQQALQSIQNMQNTLPKTTYCNQIGTQTICKGSVPAE
                     GEMTP"
                     /name="hypothetical protein"
     gene            complement(11091..11696)
                     /locus_tag="OQW59_RS28170"
                     /old_locus_tag="OQW59_28170"
     CDS             complement(11091..11696)
                     /locus_tag="OQW59_RS28170"
                     /old_locus_tag="OQW59_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_265969940.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDHSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            11791..13306
                     /locus_tag="OQW59_RS28175"
                     /old_locus_tag="OQW59_28175"
                     /pseudo=""
     CDS             11791..13306
                     /locus_tag="OQW59_RS28175"
                     /old_locus_tag="OQW59_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     Typing          1899..2418
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.502"
                     /coverage="89.0"
                     /evalue="1.9999999999999997e-142"
     Typing          9260..9779
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.502"
                     /coverage="89.0"
                     /evalue="1.9999999999999997e-142"
ORIGIN
        1 tgtaacagct actgatcata aattgattta ccataacatc cgttgtcaac atcaaaaaaa
       61 gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg gccaatggtg gcggcaagga
      121 taaccagctc gtcatcccgg atctcacaca gaatgcgata atctcccacc cgatagcgcc
      181 agaactcgcc cagctcacct ttgagcggct tccctgactg gcgaggatcg gcagcaactg
      241 caatgcgtaa atccataaaa tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta
      301 acgatttgag cgcccggtcg gaatagttaa tcgtccagac caagtgactt cctcacgtct
      361 tcagacgaat gcacggcttc atcaccacgg cgaacgcgtg cggcagtttc tgctgaaaga
      421 taataatctt ccaggtcttc cagatgcgcc agtattgctt cccgcgcata gaacgtcttt
      481 gttctgccgg tttgcttcgc cagcgagtcc agacgggact caatctcatc agataaccgg
      541 atagcaagca tataaacctc ctgaacacca tttgatatac atgtatagca atagtatcgc
      601 aatcaaccta aaagaacaaa aaataatctt gctggcccct cttgataatc cgtaacgctg
      661 tcaatatatc cagcttgagg gctttcctct taactattga aaggataacg attatgggtc
      721 ttaagccggg tccgaaacga attgccgatt caacagggga acctgataaa cgccagcgtg
      781 acaataaaaa aacgccgggg aatactgaca aattgaaacc cagcaaatcc tcaaaaaaat
      841 aagtcagtag ttaaaagccc tccggggctt tttttgtacc cgcagatcag tctgattgaa
      901 cataacatct gttgcccgca ccacctcagg cgccctcagg gcgtttctga ggactccggc
      961 ctgaaacata cacctttctg tgcccatatc gagaaaaaac gcccctgatg atttctgcgt
     1021 acctgcatgg ctatgcactc aagtgaatga agtaagattt cagtctgcga tcaccataaa
     1081 accgggaaaa agccgtatga caaaacagga atcagccgca ctgaacatgg caaaatttat
     1141 ccgggcgcag tcgctgctgc tgctcgaaaa actcgacgtg ctcgatcttg atgacgaggc
     1201 agccgactgt gagcgaatgc acgaacaggc agaagcgctt tatcagaagc tgagcgcacg
     1261 cttcggcata caggacggcg aataaagaga cgaaaagaac gctcacttag acgaaaagcg
     1321 tatccatggc aactatgtat atccaccgtg tacatagtca tgcctgacgc tttcgagcag
     1381 gtcgagcatg taggaacgac gagcttgatt ggtatcggac acaatatggc gatacgcctc
     1441 aaacataaca gcacccgcat caccgcccga acggcgaagc atagcggaaa tcatccgcac
     1501 aaagagcaga cgttcatcgg ggcaaaggtt cataagttct cctggtgaaa aaccaaacat
     1561 gaagcaccca tctattaacg tcagctgtga acgcttcatt agaagaaaat aggtaaaccc
     1621 gggaaaaaga tccaaacgtt aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa
     1681 ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt
     1741 gtcgggcaca gccccgacac cccagagccg actgtaggtc actgggcgaa cgcggcggaa
     1801 tgatgtcgga tgacgtcatt ttgcggcgtc tgccctatcc tgcatcgtat taaaaaatgc
     1861 agctatggcg cgtacggtgc caaagataag cccggagata ttccgcttcc tcgctcactg
     1921 actcgctccg ctcggtcgtt cggctgcggc gagcggtacc ggcttactgg cggggcggaa
     1981 atttcctggc atcttccagg aagaagctta acagggaagc aagaaggcca cggcgaagcc
     2041 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgaagct caaatcagtg
     2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccacctgatg gctccctctt
     2161 gcgctctcct gttcccgtca tgcggcgtcc gtgttgtggt ggaagcttta cctaaatcac
     2221 cacgccccgt tccgtttaga cagttcgctc caagctgggc tgtatgcaag aaccccccgt
     2281 tcagcccgac taccacgtcg tcccggtaac tatcacttga gtccaacccg gaacagacac
     2341 gaaaaatcgc cagtggcggt agccattggt aactgagatg tgcgagagat ttatctggag
     2401 ttcttgaagt gggagcctga gtccggctac actggaagga cagtttaggt gactcgtctc
     2461 gcacaagaca gttacctggt taagcagttc cccaactgac ctaaccttcg aaaaaccacc
     2521 tccccaggtg gttttttcgt tttcagagca agagattacg ctcagccata aatgatctca
     2581 agaagatcca ctccaacaaa ttcaaatcca acaattcata aaatgttaca tttactaatt
     2641 agtaaattta tttgttttct tgtttactga ttaagaagcc aaattcacac tgcagagaat
     2701 gatatgaaca tccctactca tctgaaacac aaaccgatta tcgctgtaga aaactatgat
     2761 cgaattgatg gcccctatgc tgatgacact gatgctatgg ggctttccgt tggaattgct
     2821 caatggaaca cccccggata taccgagcta tcagcaaaag tctggcgcaa caccggagaa
     2881 aagtggtcac ggcaatctga agagttacct cttcaccgtg tcatcgactt agcaacgctt
     2941 atctgcatag cgatggatta cagcaaacat ggtcgcttat cttccagcga cgactttccg
     3001 gtcagtcgtt caggaggtaa tcctgagtta tctcgtcata ttgatttgat gaaaaacgaa
     3061 ttaaaagaga atcacgagta tttagatgaa agtcttaagc gtttagcagc agaactaaaa
     3121 cgtttaggtt attaagtaga cgaaggtcgc tacgctcccc cgtgattttt tatataccca
     3181 ccacggggca ttaggttttt cttgtcaaaa aaggataaaa cactaaatga gaaaaacgtt
     3241 attactttgc gttccgttgt ttttacttgc tggttgcgcc caacaaaaac aacaaatgcc
     3301 agatgctaat tacgaaaagt tcgcaaaggt cgaggttgcc tctgatgcct gcttaaaatc
     3361 caacttcatt acagcacagg aagcaggtca ggcacattcc aacatatcct tatttttaag
     3421 ttcatgggcg tatgactacg ttcgttactc aaacctcttg gcgcaaggac atgaagaagt
     3481 aaaaaaaatc aagataacac aggaaggctg caaccttttg cgcgcaaaaa tctatcaaga
     3541 caacatcgag gcacagagat atcaaaaaca gatggagatg gctgcacaac aaaaagcctt
     3601 agcagatcag caggcgttac aatcaattca aaatatgcaa aacaccttac ctaaaacaac
     3661 ttactgtaac cagattggca cacagacaat ctgtaagggg tcggttccgg ctgagggcga
     3721 aatgacaccc taagcgttag ctctgtgtcg ttgcacgatg tcagcgacgg tattcttgct
     3781 gataccgagc tcgcgtgcga tccagcgata gctgcgtccc tcggccctca tcgcaaccac
     3841 cttaggcaaa agtcggtctg attttggtcg cactccggcc tgacgaccaa gcctcttacc
     3901 acgtgccttc gcaacagcaa ggcctgactt gacccgctcg ctgatgagat cccgctcaaa
     3961 ctccgcaatg ccggaaagaa acgtcgccag cattcgtcca tacggcgacg aatgatcgaa
     4021 cgccattcca ttcatggcta tcacggaaac cttccagttc tccagttcac gtagcgtatt
     4081 gagcagatcg agcgtcgagc gcccccaccg ggaaagctca gtgaccagga ttgcatcaat
     4141 ttgtctggac tgggcaagcg ccaggacttt ctttcgctcg gcccggtcga gtttagttcc
     4201 tgaacctgtt tccttaaata ttcccaccac gtcgtagccg gcacggccgg cgaaggctcg
     4261 cagatcaaat tcctggcgtt cacaagactg atccgctgtt gaaacccggc agtaaatggc
     4321 ggcacgatgt cccaattgaa ccctcctgga tttttgtatc ggaacgccct gatttatatg
     4381 ggctggctgt tgtccaaaac agactatact tcaaaaggga cgaatttgta tgtcacgacg
     4441 ccatattttc accgaacggc agcgagcagc gctgttcgat ctgcccacgg acgaactgtc
     4501 gctactgaag ttctacacgc tgggcgatga tgacctggaa aacattaggc agcgccgcag
     4561 accggaaaac aggattggct ttgccctgca actttgtgcc ttacgatatc cgggccgtgc
     4621 actggctcct ggtgagatga tcccgcgtga agtcctttcc ttcgtcggtg ctcagcttgg
     4681 agttccggct gatgcgcttc tcacttatgc cacacggcgc caaacccgtc agcagcacat
     4741 ggacacgctg cgcgaaattt acggctacaa gaccttcacg ggccgtggtg cccgtgatct
     4801 gcgggagtgg actttcggcc aggccgaaga tgccagatca aacgaggatc ttgctcatcg
     4861 ttttattgtg cggtgtcggg aaacttccac cattctgccc gcagtatcga caatcgagcg
     4921 cttgtgcgcg gatgctctgg tcgccgctga gcggcggatt gaaacgcgga ttgcggaaaa
     4981 tttaacagcg gatgttcgcg atcacctgga caaacttctg agtgaaatgc tcgccggcaa
     5041 tatcagtcgt ttcatctggc ttcgcaactt cgaggttggt aacaactcgg ctgctgctaa
     5101 ccgtttgctc gacaggctcg aatttctgcg taccctgaat atcaatcata gtgctttggc
     5161 cagcatacct gcccatcgca ttgcccggct gcgtcggcag ggtgaacgct acttcaccga
     5221 cggtttgcgt gacatcactt cggaccgccg ctgggcgatc cttgccgtct gtgttgtgga
     5281 gtgggaagcg gcgattgctg atgccatagt cgaaacccat gacaggatcg taggaaaaac
     5341 ctggcgggaa gcgaagcgcc agcatgacga aacaatttcc ggctctaaag ccacactcac
     5401 ggatacgatc cgtaccttca ccgcgctggg agcttcgttg cttgaggccc gcagtgacgg
     5461 aaccccgctg gagatggctg tcgccagttc ggttgcatgg gaccggctcg ctcaactggt
     5521 agcgacaggg actcaactca gcaacacgct agccgatgag cctcttgcat atgtcgggca
     5581 gggataccat cgctttcgtc gttatgcgcc ccgcatgttg cgctgtctga agctcgaagc
     5641 cgcgccggtc gccggaccat tggtagcagc agctttgtcg atcggagaga tgaaaggtgt
     5701 tgcatcgcca gaaaggcgtt tcctgcggcc cagctccaaa tggaaccgtc atttacgagc
     5761 tcaggaaaaa ggagataccc gtctttggga agtggcggta ctctttcacc tccgggatgc
     5821 ttttcgttcc ggagatgtct ggctcgctca ttcgcgccgc tatggtgacc tcaagcaggt
     5881 actggtgccg atgatcgcgg cgcaggaaaa tgcaaaactg gccgtgcctt ccaacccaca
     5941 ggattggctg gcagacagaa aggcgcgact cacgatcgct cttaagcggc tggcccgggc
     6001 tgcccgtaac ggcactattc cgcacggtag catagaagat ggaacgttgc ggatcgacag
     6061 gttgacagca gacgtgccgg atggtgccga ggcactcata ctggatctgt atcgccgaat
     6121 gccgtccgtt cggattaccg acatgctgct tgaagttgat gcagcccttg gtttcacaga
     6181 tgcgtttacc catctgagaa ccggggctcc atgtcgcgac cggatcggtc tgctcaacgt
     6241 cctgctcgct gaagggctca atctgggcct gcgtaagatg gcggaagcta caaacacgca
     6301 tgattactgg cagctctcac gccttgcccg ctggcatgtt gaaagcgaag ccatgaacca
     6361 ggcattggca attgtggtgg ccgcgcaggg taaactgccg atgtcacgcg tctgggggat
     6421 gggcacgtca gcatcgagcg atggtcagtt tttcccgaca gcgcggcatg gcgaagccat
     6481 gaacatggtc aatgccaaat atggttctgt tcccggcctc aaagcgtata ctcacgtaag
     6541 cgaccagttc gcgccattcg cttgtcagtc gatcccggcg accgtgagcg aggcaccgta
     6601 tattctcgat ggactactga tgaacgaggt cggtcgccat gttcgcgaac agtatgccga
     6661 tacagcagga ttcaccgacc atttgttcgg agccagtagc ctgctcggct acaatctcgt
     6721 tctgcgaatc agggatctgc catcgaagcg gttgtacgta tttaatcccg atacgacccc
     6781 cagggagtta cgcaagttgg taggtggaaa agcccgggag gatcttatcg ttgcgaactg
     6841 gcctgatatt ttccgttgtg ccgcgacgat gaccgctggc aaaatcaggc ccagccaact
     6901 cctgcgcaag ctcgcttctt acccacgaca aaacaacctt gcagttgcgc ttcgtgaagt
     6961 tggtcgtatt gaacggaccc ttttcattat tgagtggatc ctggatacgg acatgcagcg
     7021 gcgtgctcag atcggtctta acaagggaga ggcccaccat gcgctcaaaa atgcgctccg
     7081 tatcgggagg cagggggaaa ttcgcgatcg cacgacagag gggcagcact accgaatcgc
     7141 tgggctcaat ttattgactg cggtgatcat ttactggaat accgtccatc ttggtcatgc
     7201 cgtcacggag cggcggaacg aagggttgga tgttccccct gaatttcttc cccacatatc
     7261 cccattgggc tgggcgcaca ttctactgac tggcgaatat ctttggccca aggaaccgaa
     7321 agcttagggt gtcatttcgc cctcagccgg aaccgacccc ttcaacagct actgatcata
     7381 aattgattta ccataacatc cgttgtcaac atcaaaaaaa gcccgcatag cgggcttcag
     7441 tcgtaaactt cgcggcgatg gccaatggtg gcggcaagga taaccagctc gtcatcccgg
     7501 atctcacaca gaatgcgata atctcccacc cgatagcgcc agaactcgcc cagctcacct
     7561 ttgagcggct tccctgactg gcgaggatcg gcagcaactg caatgcgtaa atccataaaa
     7621 tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta acgatttgag cgcccggtcg
     7681 gaatagttaa tcgtccagac caagtgactt cctcacgtct tcagacgaat gcacggcttc
     7741 atcaccacgg cgaacgcgtg cggcagtttc tgctgaaaga taataatctt ccaggtcttc
     7801 cagatgcgcc agtattgctt cccgcgcata gaacgtcttt gttctgccgg tttgcttcgc
     7861 cagcgagtcc agacgggact caatctcatc agataaccgg atagcaagca tataaacctc
     7921 ctgaacacca tttgatatac atgtatagca atagtatcgc aatcaaccta aaagaacaaa
     7981 aaataatctt gctggcccct cttgataatc cgtaacgctg tcaatatatc cagcttgagg
     8041 gctttcctct taactattga aaggataacg attatgggtc ttaagccggg tccgaaacga
     8101 attgccgatt caacagggga acctgataaa cgccagcgtg acaataaaaa aacgccgggg
     8161 aatactgaca aattgaaacc cagcaaatcc tcaaaaaaat aagtcagtag ttaaaagccc
     8221 tccggggctt tttttgtacc cgcagatcag tctgattgaa cataacatct gttgcccgca
     8281 ccacctcagg cgccctcagg gcgtttctga ggactccggc ctgaaacata cacctttctg
     8341 tgcccatatc gagaaaaaac gcccctgatg atttctgcgt acctgcatgg ctatgcactc
     8401 aagtgaatga agtaagattt cagtctgcga tcaccataaa accgggaaaa agccgtatga
     8461 caaaacagga atcagccgca ctgaacatgg caaaatttat ccgggcgcag tcgctgctgc
     8521 tgctcgaaaa actcgacgtg ctcgatcttg atgacgaggc agccgactgt gagcgaatgc
     8581 acgaacaggc agaagcgctt tatcagaagc tgagcgcacg cttcggcata caggacggcg
     8641 aataaagaga cgaaaagaac gctcacttag acgaaaagcg tatccatggc aactatgtat
     8701 atccaccgtg tacatagtca tgcctgacgc tttcgagcag gtcgagcatg taggaacgac
     8761 gagcttgatt ggtatcggac acaatatggc gatacgcctc aaacataaca gcacccgcat
     8821 caccgcccga acggcgaagc atagcggaaa tcatccgcac aaagagcaga cgttcatcgg
     8881 ggcaaaggtt cataagttct cctggtgaaa aaccaaacat gaagcaccca tctattaacg
     8941 tcagctgtga acgcttcatt agaagaaaat aggtaaaccc gggaaaaaga tccaaacgtt
     9001 aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa ttctttcccg gacggcgcgg
     9061 ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt gtcagggcac agccccgaca
     9121 ccccagagcc gactgtaggt cactgggcga acgcggcgga atgatgtcgg atgacgtcat
     9181 tttgcggcgt ctgccctatc ctgcatcgta ttaaaaaatg cagctatggc gcgtacggtg
     9241 ccaaagataa gcccggagat attccgcttc ctcgctcact gactcgctcc gctcggtcgt
     9301 tcggctgcgg cgagcggtac cggcttactg gcggggcgga aatttcctgg catcttccag
     9361 gaagaagctt aacagggaag caagaaggcc acggcgaagc cgtttttcca taggctccgc
     9421 ccccctgaca agcatcacga aatctgaagc tcaaatcagt ggtggcgaaa cccgacagga
     9481 ctataaagat accaggcgtt tccacctgat ggctccctct tgcgctctcc tgttcccgtc
     9541 atgcggcgtc cgtgttgtgg tggaagcttt acctaaatca ccacgccccg ttccgtttag
     9601 acagttcgct ccaagctggg ctgtatgcaa gaaccccccg ttcagcccga ctaccacgtc
     9661 gtcccggtaa ctatcacttg agtccaaccc ggaacagaca cgaaaaatcg ccagtggcgg
     9721 tagccattgg taactgagat gtgcgagaga tttatctgga gttcttgaag tgggagcctg
     9781 agtccggcta cactggaagg acagtttagg tgactcgtct cgcacaagac agttacctgg
     9841 ttaagcagtt ccccaactga cctaaccttc gaaaaaccac ctccccaggt ggttttttcg
     9901 ttttcagagc aagagattac gctcagccat aaatgatctc aagaagatcc actccaacaa
     9961 attcaaatcc aacaattcat aaaatgttac atttactaat tagtaaattt atttgttttc
    10021 ttgtttactg attaagaagc caaattcaca ctgcagagaa tgatatgaac atccctactc
    10081 atctgaaaca caaaccgatt atcgctgtag aaaactatga tcgaattgat ggcccctatg
    10141 ctgatgacac tgatgctatg gggctttccg ttggaattgc tcaatggaac acccccggat
    10201 ataccgagct atcagcaaaa gtctggcgca acaccggaga aaagtggtca cggcaatctg
    10261 aagagttacc tcttcaccgt gtcatcgact tagcaacgct tatctgcata gcgatggatt
    10321 acagcaaaca tggtcgctta tcttccagcg acgactttcc ggtcagtcgt tcaggaggta
    10381 atcctgagtt atctcgtcat attgatttga tgaaaaacga attaaaagag aatcacgagt
    10441 atttagatga aagtcttaag cgtttagcag cagaactaaa acgtttaggt tattaagtag
    10501 acgaaggtcg ctacgctccc ccgtgatttt ttatataccc accacggggc attaggtttt
    10561 tcttgtcaaa aaaggataaa acactaaatg agaaaaacgt tattactttg cgttccgttg
    10621 tttttacttg ctggttgcgc ccaacaaaaa caacaaatgc cagatgctaa ttacgaaaag
    10681 ttcgcaaagg tcgaggttgc ctctgatgcc tgcttaaaat ccaacttcat tacagcacag
    10741 gaagcaggtc aggcacattc caacatatcc ttatttttaa gttcatgggc gtatgactac
    10801 gttcgttact caaacctctt ggcgcaagga catgaagaag taaaaaaaat caagataaca
    10861 caggaaggct gcaacctttt gcgcgcaaaa atctatcaag acaacatcga ggcacagaga
    10921 tatcaaaaac agatggagat ggctgcacaa caaaaagcct tagcagatca gcaggcgtta
    10981 caatcaattc aaaatatgca aaacacctta cctaaaacaa cttactgtaa ccagattggc
    11041 acacagacaa tctgtaaggg gtcggttccg gctgagggcg aaatgacacc ctaagcgtta
    11101 gctctgtgtc gttgcacgat gtcagcgacg gtattcttgc tgataccgag ctcgcgtgcg
    11161 atccagcgat agctgcgtcc ctcggccctc atcgcaacca ccttaggcaa aagtcggtct
    11221 gattttggtc gcactccggc ctgacgacca agcctcttac cacgtgcctt cgcaacagca
    11281 aggcctgact tgacccgctc gctgatgaga tcccgctcaa actccgcaat gccggaaaga
    11341 aacgtcgcca gcattcgtcc atacggcgac gaatgatcga acgccattcc attcatggct
    11401 atcacggaaa ccttccagtt ctccagttca cgtagcgtat tgagcagatc gagcgtcgag
    11461 cgcccccacc gggaaagctc agtgaccagg attgcatcaa tttgtctgga ctgggcaagc
    11521 gccaggactt tctttcgctc ggcccggtcg agtttagttc ctgaacctgt ttccttaaat
    11581 attcccacca cgtcgtagcc ggcacggccg gcgaaggctc gcagatcaaa ttcctggcgt
    11641 tcacaagact gatccgctgt tgaaacccgg cagtaaatgg cggcacgatg tcccaattga
    11701 accctcctgg atttttgtat cggaacgccc tgatttatat gggctggctg ttgtccaaaa
    11761 cagactatac ttcaaaaggg acgaatttgt atgtcacgac gccatatttt caccgaacgg
    11821 cagcgagcag cgctgttcga tctgcccacg gacgaactgt cgctactgaa gttctacacg
    11881 ctgggcgatg atgacctgga aaacattagg cagcgccgca gaccggaaaa caggattggc
    11941 tttgccctgc aactttgtgc cttacgatat ccgggccgtg cactggctcc tggtgagatg
    12001 atcccgcgtg aagtcctttc cttcgtcggt gctcagcttg gagttccggc tgatgcgctt
    12061 ctcacttatg ccacacggcg ccaaacccgt cagcagcaca tggacacgct gcgcgaaatt
    12121 tacggctaca agaccttcac gggccgtggt gcccgtgatc tgcgggagtg gactttcggc
    12181 caggccgaag atgccagatc aaacgaggat cttgctcatc gttttattgt gcggtgtcgg
    12241 gaaacttcca ccattctgcc cgcagtatcg acaatcgagc gcttgtgcgc ggatgctctg
    12301 gtcgccgctg agcggcggat tgaaacgcgg attgcggaaa atttaacagc ggatgttcgc
    12361 gatcacctgg acaaacttct gagtgaaatg ctcgccggca atatcagtcg tttcatctgg
    12421 cttcgcaact tcgaggttgg taacaactcg gctgctgcta accgtttgct cgacaggctc
    12481 gaatttctgc gtaccctgaa tatcaatcat agtgctttgg ccagcatacc tgcccatcgc
    12541 attgcccggc tgcgtcggca gggtgaacgc tacttcaccg acggtttgcg tgacatcact
    12601 tcggaccgcc gctgggcgat ccttgccgtc tgtgttgtgg agtgggaagc ggcgattgct
    12661 gatgccatag tcgaaaccca tgacaggatc gtaggaaaaa cctggcggga agcgaagcgc
    12721 cagcatgacg aaacaatttc cggctctaaa gccacactca cggatacgat ccgtaccttc
    12781 accgcgctgg gagcttcgtt gcttgaggcc cgcagtgacg gaaccccgct ggagatggct
    12841 gtcgccagtt cggttgcatg ggaccggctc gctcaactgg tagcgacagg gactcaactc
    12901 agcaacacgc tagccgatga gcctcttgca tatgtcgggc agggatacca tcgctttcgt
    12961 cgttatgcgc cccgcatgtt gcgctgtctg aagctcgaag ccgcgccggt cgccggacca
    13021 ttggtagcag cagctttgtg cgctcaaaaa tgcgctccgt atcgggaggc agggggaaat
    13081 tcgcgatcgc acgacagagg ggcagcacta ccgaatcgct gggctcaatt tattgactgc
    13141 ggtgatcatt tactggaata ccgtccatct tggtcatgcc gtcacggagc ggcggaacga
    13201 agggttggat gttccccctg aatttcttcc ccacatatcc ccattgggct gggcgcacat
    13261 tctactgact ggcgaatatc tttggcccaa ggaaccgaaa gcttagggtg tcatttcgcc
    13321 ctcagccgga accgac
//
