LOCUS       NZ_CP110530             4581 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Klebsiella pneumoniae strain WS_29-2 plasmid pRWS292.s7, complete
            sequence.
ACCESSION   NZ_CP110530
VERSION     NZ_CP110530.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31525422
            Assembly: GCF_029542185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     dissemination of Carbapenem-producing Enterobacteriaceae in the
            natural water sources, Central Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2022) Collage of Public Health Science,
            Chulalongkorn University, Phayathai Road, Bangkok, Bangkok 10330,
            Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-SEP-2022
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 440.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029542185.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:56:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,142
            CDSs (total)                      :: 6,019
            Genes (coding)                    :: 5,776
            CDSs (with protein)               :: 5,776
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 88 of 243
            Pseudo Genes (incomplete)         :: 163 of 243
            Pseudo Genes (internal stop)      :: 37 of 243
            Pseudo Genes (multiple problems)  :: 41 of 243
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110530.1.
            Bacteria and source DNA available from water samples PH lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WS_29-2"
                     /isolation_source="natural water sources close by farm or
                     community"
                     /db_xref="taxon:573"
                     /plasmid="pRWS292.s7"
                     /geo_loc_name="Thailand: Ratchaburi"
                     /lat_lon="13.53 N 99.81 E"
                     /collection_date="2022-05-01"
                     /collected_by="Chulalongkorn University"
     gene            join(3949..4581,1..255)
                     /locus_tag="OM422_RS30630"
                     /old_locus_tag="OM422_30610"
     CDS             join(3949..4581,1..255)
                     /locus_tag="OM422_RS30630"
                     /old_locus_tag="OM422_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_250950583.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIATAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            252..950
                     /locus_tag="OM422_RS30635"
                     /old_locus_tag="OM422_30615"
     CDS             252..950
                     /locus_tag="OM422_RS30635"
                     /old_locus_tag="OM422_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(978..1355)
                     /locus_tag="OM422_RS30640"
                     /old_locus_tag="OM422_30620"
     CDS             complement(978..1355)
                     /locus_tag="OM422_RS30640"
                     /old_locus_tag="OM422_30620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_277696618.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFASVSVEQEEEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRP"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1355..1546)
                     /locus_tag="OM422_RS30645"
                     /old_locus_tag="OM422_30625"
     CDS             complement(1355..1546)
                     /locus_tag="OM422_RS30645"
                     /old_locus_tag="OM422_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_060568275.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLTLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2577..2693
                     /locus_tag="OM422_RS30650"
                     /old_locus_tag="OM422_30630"
     CDS             2577..2693
                     /locus_tag="OM422_RS30650"
                     /old_locus_tag="OM422_30630"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_016947047.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     gene            complement(2820..3170)
                     /locus_tag="OM422_RS30655"
                     /old_locus_tag="OM422_30635"
     CDS             complement(2820..3170)
                     /locus_tag="OM422_RS30655"
                     /old_locus_tag="OM422_30635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_277696617.1"
                     /translation="MQVGESVVNAIVEYHAQSSEEAHAEENRMFLRYLHELTGTQRVAV
                     EVARKYFSPVRADMSYLFLVAGTAPGATVLLQNCPHLTGLDGPFFYAFNVRGPFLGRNH
                     LLCSGVHSHAYA"
                     /name="hypothetical protein"
     gene            3735..3956
                     /locus_tag="OM422_RS30660"
                     /old_locus_tag="OM422_30640"
     CDS             3735..3956
                     /locus_tag="OM422_RS30660"
                     /old_locus_tag="OM422_30640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231537463.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVALSTVTRWLEKNG"
                     /name="hypothetical protein"
     Typing          1595..2075
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.86"
                     /coverage="91.0"
                     /evalue="7.770000000000004e-156"
ORIGIN
        1 gtgaccgatc gcggcgtgtt ttaccagagt cagtacgcca ttggcggagg ccagcgctgg
       61 tcaaacgcct tcagtgccgc ctccatgcgt accctgggct ggtcacgggg ggcacacggc
      121 gtgcgtcaca gctacgccca gcaacggatg caggagctgc aaaaactcgg tctggttcgt
      181 gatatggccc tgcgcaccgt cagccaggaa atgggccatt ttcgtccgga aatcacggag
      241 acatacctgc gatgacaaga acatttattt tattgtgtac cggtgttttc gccatcgcgt
      301 acgccctgta tgagctgaat cactatggcg tttactatcc ggacgtcagc aatcctgagg
      361 cgtttactgg ctggaaggcg gtcaccgaac tcggtcccgc tatcctggca acggggatcg
      421 cagccacagc gttctcgctg tggctgttca ttttgttttt cagaggctca gagtcccgcc
      481 ggatacagcg cgagataagc ggatatcagc aggcactgag ggaggcagaa gaggctcaca
      541 gagccagaca aaataccctt attcagaatc acaacctgga gacagcggcc ctgcgaagcc
      601 actacattgg tgctgaggat gtactgcggg aagcgtttgc gcaaaaagaa agcctcttgc
      661 ttcaggcggt gcagaatgcc cggaaagaag ccagaaagga gctggaagaa cagagggagg
      721 cactgaatca gcgcgaatcc atcctgcaga aaaaggagct ggcgctggaa acaaccctgg
      781 agaaggcccg gaaatatgtg gccggggagc ggcataacac cgagctggcc cgtctggaag
      841 cagagaaagc agaggaaaga cggagaaatg cctgcgcgac ggcggaacgg cgcagacgga
      901 aactggaaaa actccaggcc actggcggaa cagcagggca aaaacactga ggcatttcag
      961 gactgccaga aacgccatca gggccgggtc gtgttctgcc gctgatcttc ccggttctcc
     1021 agcaacaaca tcgcctgttt caggctgtcg atatgtgaat cttttgaggc aagaagtgtg
     1081 ttcaggtgtt caatttcctg tttcagagta tgaatgacct gttcattatg gtcagaagtg
     1141 actgtgtcac gctgtccact gacctgttct tcctcctgtt caacggatac ggacgcaaag
     1201 gcaccaaaca cccgcaaaag ctctgaagta tcgagcattt tcgcattccg ttcaccccga
     1261 caggtggaca gttcaccggc tttaattagc ctgtgcaggg ttgtcctgct cttacccgtc
     1321 aggcgtgcag cctcactgat cgacacgttc gccatcagtc accctcctgt agtgcatcca
     1381 gtctgagggt cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt
     1441 cagcctccgc atcgagatca agctcgttaa gcttctccag caggagcagg gactgattct
     1501 ggatgaattt agccatgttc agcgcagttt tctgttgttt cgtcataagg ggcctcagag
     1561 cgtcagtgct cagtgtcaaa ccagtctctt ttccgcttcc tcgctcactc agtcgctacg
     1621 ctcctgccgt tcggctgcgg cgagcggacg gggcacacag acagaaaagt catcgccacc
     1681 acccggaaaa ggaaaagaac agacgggaga aggccacggc gttgccgttt ttccataggc
     1741 tccgcccccc tgacaagcat cacaaaatct gacgctcaaa tcagcggtgg cgaaacccga
     1801 caggactata aagatcccag gcgtttcccc ctggtggctc cctcgtgcgc tctcctgttc
     1861 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
     1921 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1981 gttcagtccg actaccacgc tcttttcggt aactatcgtc ttgagtccaa cccgaaaagg
     2041 cacgacaaat cgccattggc ggtagccact ggtaaagggg tgtacaagag ataactggta
     2101 cagagttctt gaagttgaag cctaactacg gctaaactgg aaggacacat ttggtggcag
     2161 cggtcttgta caccctgtta ccacggttca gaagttcccc aacttgctga accttcgaaa
     2221 aaccgccctg caaggcggtt ttttcgttta cagagcaaga gattacgacg aggctgaatg
     2281 aatctcaaga tttcatttag cctgccaccc gatcattcaa agttttggcg tagttctgac
     2341 ccgctccccg tgatttgtta catacccacc acagggcatt agttttttta tttttttgat
     2401 gatatgtaca aaatcagtgg ttaggaataa tcttagcagc cttaacaaac acatacaaaa
     2461 acagcggtta tgtttcatct caccacaata agtaacgaca gtatcatgct aaaaaaagat
     2521 gatattatct ttatgaaatc ataacaggct gtttttatta aattaatcag gcatttatgg
     2581 actggatttc acttgctatc gccattgctc tacttgttgt agctgtctac tcaggaatct
     2641 catacttcag agaaaaaaaa acaatcagtc gtacatttag gcgtaggcga tagcgcccta
     2701 tttaccataa tcccttctta cgcgcaccgc gaaaacggcc tcagggcgtt tctggtggtt
     2761 aacgggcaaa aggtggtggc aagtacaccg aaaaccgcca gaaacgcgcc tgatttattt
     2821 tacgcatatg catggctatg cacaccactg catagtaggt gatttcggcc gagaaaaggg
     2881 ccccggacgt taaaagcata aaagaacggg ccatccaggc ccgtcaaatg aggacaattc
     2941 tgtaaaagga ccgtcgcacc tggtgcggtg ccggcgacaa gaaaaaggta gctcatatca
     3001 gccctcaccg gcgaaaaata tttcctggcc acctcaaccg ccacacgctg tgttcccgtt
     3061 aactcgtgca gataacgcag gaacatcctg ttttcctctg catgcgcctc ctcactgctc
     3121 tgtgcgtggt attccactat cgcgttcacc acggactcac cgacctgcat cgtctgacct
     3181 catctgcact ttcatcacac cgtttcagat cccgcctcct cgccatgatg tgaaacggtg
     3241 accgggcagg caaatttttt gtctgtccgg tcaccgttgg cggggtccgg ggcggtcagt
     3301 cccggtgttt tttattccgc caaatcagca cctgcgacac ccgctatgta cgacgcataa
     3361 atgcgcctgt acatagcggg ggtcgctatt gagcggagtc aggcgcaaca tttaaaaaac
     3421 aacattcaca cgcacgaaaa acctggctag tatttagtca aactatgagc ccgcgcactc
     3481 tgacgcgcac ctgcctaccg gcagcaggta aaacagccgg gtccctccgg ggatactgta
     3541 ccgggcagcc caatccgaga acactgagac aacagcccaa atctgtcaga cccacaaccg
     3601 ggtcaagtca ttctctgact aaagagacgt tgagccgcga gcagtccggg cttaaccacg
     3661 ttacggttta aacgagggcg atcggcgcag gccgcagtgg atttccactg atgttcttac
     3721 acaccggagt acagatgcaa tttgataccg ctgccgaact ggccgccctg caggcgcaga
     3781 cccgccgtat ccgccaggtc cgctaccgtc cctcccgcct tgaccgctat accggcgaac
     3841 tgctgagcct gtaccaggcc ggggccagtg cagcggagct gcaacgctgg ctccgggccc
     3901 ggagaatcaa agtggcgttg tccacggtta cccgctggct ggaaaaaaat ggctaaattc
     3961 gccagcgctg cccgccaggc ggctggcgtg ataaaaccac tccagggaaa tattctgcgc
     4021 tctgtcggca cagtccggaa ttacgagcag ggactgaccc gcgtcgcaga gtggatgaaa
     4081 gccaaccgga ttaatcacgg cgatttacgc acactgacac cggcgcaggc catcacctac
     4141 ctggagcagc gcggggagca ggttggccag aaaacgctcg atatggaacg ccaggccatc
     4201 caggccatga tgatccacgt gacgcagacc ctccctgccg gcgaacacct gccggtggtg
     4261 aaatcggagc aggcacagat cctgagcagc cgggcctaca gcccggacca ggtagccctg
     4321 attgccactg cacagcagcc cgccaacgct ctggccaccg cgattgccct ggccgccgga
     4381 ttacgcgccc atgaactgct gaccctcgcg ccccgccagg agcgagcagc ggatccccgt
     4441 ccggcagcgg acagtaaatt tgccggtcgg gagggtgtga tttacaccgt tcagggcaaa
     4501 ggcggcctgg tcagggaagt tctgctgccg tcacgcctgg ctgaacgtct cgagcatgtc
     4561 aggctggcca gcccggtccg g
//
