LOCUS       NZ_CP110522             3347 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Klebsiella pneumoniae strain WS_29-1 plasmid pRWS291.s8, complete
            sequence.
ACCESSION   NZ_CP110522
VERSION     NZ_CP110522.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31525373
            Assembly: GCF_029542165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     dissemination of Carbapenem-producing Enterobacteriaceae in the
            natural water sources, Central Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2022) Collage of Public Health Science,
            Chulalongkorn University, Phayathai Road, Bangkok, Bangkok 10330,
            Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-SEP-2022
            Assembly Method                   :: unitycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 468.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029542165.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:43:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,132
            CDSs (total)                      :: 6,010
            Genes (coding)                    :: 5,766
            CDSs (with protein)               :: 5,766
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 89 of 244
            Pseudo Genes (incomplete)         :: 162 of 244
            Pseudo Genes (internal stop)      :: 37 of 244
            Pseudo Genes (multiple problems)  :: 40 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110522.1.
            Bacteria and source DNA available from water samples PH lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WS_29-1"
                     /isolation_source="natural water sources close by farm or
                     community"
                     /db_xref="taxon:573"
                     /plasmid="pRWS291.s8"
                     /geo_loc_name="Thailand: Ratchaburi"
                     /lat_lon="13.53 N 99.81 E"
                     /collection_date="2022-05-01"
                     /collected_by="Chulalongkorn University"
     gene            200..>280
                     /locus_tag="OM421_RS30615"
                     /old_locus_tag="OM421_30605"
                     /pseudo=""
     CDS             200..>280
                     /locus_tag="OM421_RS30615"
                     /old_locus_tag="OM421_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(554..1300)
                     /locus_tag="OM421_RS30620"
                     /old_locus_tag="OM421_30610"
     CDS             complement(554..1300)
                     /locus_tag="OM421_RS30620"
                     /old_locus_tag="OM421_30610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128312535.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGNGNGREVSLVNGGFK
                     RFITGDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKKEAESRSGKWT
                     LSNVKIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(1500..1670)
                     /locus_tag="OM421_RS30625"
                     /old_locus_tag="OM421_30615"
     CDS             complement(1500..1670)
                     /locus_tag="OM421_RS30625"
                     /old_locus_tag="OM421_30615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771094.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHP"
                     /name="hypothetical protein"
     gene            complement(2521..2922)
                     /locus_tag="OM421_RS30630"
                     /old_locus_tag="OM421_30620"
     CDS             complement(2521..2922)
                     /locus_tag="OM421_RS30630"
                     /old_locus_tag="OM421_30620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2922..3113)
                     /locus_tag="OM421_RS30635"
                     /old_locus_tag="OM421_30625"
     CDS             complement(2922..3113)
                     /locus_tag="OM421_RS30635"
                     /old_locus_tag="OM421_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049139654.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEELHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(91..290)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.015"
                     /coverage="100.0"
                     /evalue="1.08e-90"
ORIGIN
        1 gtggtggcga aacccgacag gactataaag atatcaggcg tttccccctg atggctccct
       61 cgtgcgctct cctgttccga ccctgccgct taccagatac ctgtccgcct ttctcccttc
      121 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
      181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      241 cggtaactat cgtcttgagt ccaacccggt aagacacgac taatcgccat tgacaacggc
      301 cactggtaga caggatgtgc gagagatgta tcgagagttc ttgaagtggt ggcctgactg
      361 cggctacact gaaaggacag atttggtgac atgtctcgca caagactgtt accacggttc
      421 agaagttccc caacttactg aaccttcgaa aaaccacctc cccaggtggt tttttcgttt
      481 tccgagaaag agattacgct ttgagcgaat gaatctcaag aagatcatgg catttgatga
      541 acaaatgagt aagctactca gattgataga aaaaatgccc cttatacaag tgatatattt
      601 tccaaaactg atactctatt ttaacattag aaagcgtcca tttcccactc cgcgattcag
      661 cctctttttt aatatcagca atacttggga tcaccccatt tgaaataaca aaatcgtcca
      721 accaatccca catttctcgc cgagatgatt taaatacaac aggagctttc ccatatgagc
      781 aagttggcat aaaatccccc gtaataaatc ttttaaaccc cccattcaca agagaaactt
      841 ctcttccatt accattacca ttaccattac cattaccatt accatttttc atcgtagagc
      901 tatcaggttc cttaattgtc acactgccat caggcccaac aaatatccag tgtaaattca
      961 atttactaac agcaagaatg ctcgtttctt ctattaatct ttcatactgt tttattcttg
     1021 gaacagcaat agcataatta atattatctt ttgtcattct ctgcactaat tgtccaagac
     1081 aagttcgaaa atccaacccc ggctgtgatg tttttccttt tacttcaata tgccacacaa
     1141 catccctaga ttctttatca cgacaaataa ggtcaatata agaacccgta tctatatcga
     1201 acccttgtcg aaaaaaatat ttttctaaag aagcttgaac aaattcctcg gagggatatt
     1261 caccacctag taagtacctt ctcgtcacca taagagccac aacccctacg catcctattt
     1321 actataacgt catattttaa gagttcccac aaggggagct ctctgtacga ttacgagtga
     1381 tcattcgata tatttcgttg aaatccatac catgaatcaa catcttcgcc gccacatagc
     1441 ctttctctaa tcaatttgag gaaagtatcc ctagccacag ctgatgctag aaaaagagct
     1501 tatggatgat gattagagct cacgacccaa gcatcacccc gaccaccctc aacacactga
     1561 tacaacccca tatcacttac attctgaacg gaatcataaa gagccataac ctcctcataa
     1621 tcgccatcaa catcatctaa ccacgcatca aaattagtag cagtcgccat gccaatcctc
     1681 cattttttag ttttcaggag ttaatttaca tagtgatagg ggctgttaag caaataagtt
     1741 tttacctttt ttgcctcatg ctctaaaagt gagcaagaca ggcatttacc ataacgtccc
     1801 attacgcgca ccgccagaac ggcctcaggg cgtttctgac gggaaaccgg taaaacaggc
     1861 aacaaccccc ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag
     1921 tatgcacgca agtgcatagc gcgaaaattc acgccattca gccagggtaa gtcctgtttc
     1981 cggcgtactt cccatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag
     2041 cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatccgg ttcgtcgacg
     2101 gccggcaatc acgttatggc aagtcaccga acgactcacc acacgattca ccggcgactc
     2161 ttcactagag tcgtacccag agtcgccacg cctaaaaaac aaccaaacgg ggagtcgggt
     2221 ctagagtcgg gtcgttatta actcattgtt tttatagtcc tgagtcgtta ccgactctat
     2281 gacgactcgc cggggactct atgacgagtc gtcatagagt cggatctata taaatcaaat
     2341 ttaaaaaacg aaattttaca gacttaaact actgatatta aatacaaatc cgcaaaattt
     2401 taattttgcg tagtgtgtgg atatttttag tggtgaggag cgttcagaaa tccggcgatt
     2461 ttttggaagg ttcaggaact ccccgggatg gccggagagg aaaacggaag catgacgggg
     2521 tcaggatttt ttccagaatt tccaccatgg cgccggggat tcagttaatt ttgcctggtg
     2581 attttcaaga aggagcatgg cctgtttcag gctgtcaatg tgcgaatcct tggacaaaag
     2641 taagatattc aggtgttcaa tctcctgttt cagatgacga atcacctgtt cactctggtc
     2701 agcagaggct gtatcacgct gttcggagac ctgtccagaa tggtgttcac ccttcggttg
     2761 ctcaaaggga ccaaagacac gtaaaagctc tgaagtatct accatttttg cattgcgctc
     2821 accactacag gtggacaggt cacctgtttt aattaacctg tgcagtgttg ttctactctt
     2881 acccgtcagt cgggcagcct cactaataga cactctggcc atcagttacc atcctgtaat
     2941 gcatccagtc tcgaggacag tgtacggaaa agatgctccg catcatcgtg gagttcctca
     3001 caaaggtcgg cctcgacatc gagatcaagc tcgttaagct tctccagcag gagcagagac
     3061 tgattctgga tgaatttagc catgttcagc gctgttttct gttgtttcgt cataagaggc
     3121 ctcagagcgt cagtgctcaa tgtagggatg ttctcttttc cgcttcctcg ctcactcagt
     3181 cgctacgctc ctgccgttcg gctgcggcga gcggcaggag catgcgtata aaagctcgag
     3241 aaaaccgagc gatcgcgtga tggggagaag gagaggccgc acagcggccg tttttccata
     3301 ggctccgccc cccctgacaa gcatcacaga aactgacgct caaatca
//
