LOCUS       NZ_CP110521             4581 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Klebsiella pneumoniae strain WS_29-1 plasmid pRWS291.s7, complete
            sequence.
ACCESSION   NZ_CP110521
VERSION     NZ_CP110521.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31525373
            Assembly: GCF_029542165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     dissemination of Carbapenem-producing Enterobacteriaceae in the
            natural water sources, Central Thailand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Songsaeng,W., Prapasarakul,N., Am-In,N., Wongsurawat,T. and
            Sirichokchatchawan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2022) Collage of Public Health Science,
            Chulalongkorn University, Phayathai Road, Bangkok, Bangkok 10330,
            Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-SEP-2022
            Assembly Method                   :: unitycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 468.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029542165.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:43:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,132
            CDSs (total)                      :: 6,010
            Genes (coding)                    :: 5,766
            CDSs (with protein)               :: 5,766
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 89 of 244
            Pseudo Genes (incomplete)         :: 162 of 244
            Pseudo Genes (internal stop)      :: 37 of 244
            Pseudo Genes (multiple problems)  :: 40 of 244
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110521.1.
            Bacteria and source DNA available from water samples PH lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WS_29-1"
                     /isolation_source="natural water sources close by farm or
                     community"
                     /db_xref="taxon:573"
                     /plasmid="pRWS291.s7"
                     /geo_loc_name="Thailand: Ratchaburi"
                     /lat_lon="13.53 N 99.81 E"
                     /collection_date="2022-05-01"
                     /collected_by="Chulalongkorn University"
     gene            complement(join(4199..4581,1..505))
                     /locus_tag="OM421_RS30580"
                     /old_locus_tag="OM421_30570"
     CDS             complement(join(4199..4581,1..505))
                     /locus_tag="OM421_RS30580"
                     /old_locus_tag="OM421_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_250950583.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIATAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            complement(498..719)
                     /locus_tag="OM421_RS30585"
                     /old_locus_tag="OM421_30575"
     CDS             complement(498..719)
                     /locus_tag="OM421_RS30585"
                     /old_locus_tag="OM421_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231537463.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVALSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            1284..1634
                     /locus_tag="OM421_RS30590"
                     /old_locus_tag="OM421_30580"
     CDS             1284..1634
                     /locus_tag="OM421_RS30590"
                     /old_locus_tag="OM421_30580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_277696617.1"
                     /translation="MQVGESVVNAIVEYHAQSSEEAHAEENRMFLRYLHELTGTQRVAV
                     EVARKYFSPVRADMSYLFLVAGTAPGATVLLQNCPHLTGLDGPFFYAFNVRGPFLGRNH
                     LLCSGVHSHAYA"
                     /name="hypothetical protein"
     gene            complement(1761..1877)
                     /locus_tag="OM421_RS30595"
                     /old_locus_tag="OM421_30585"
     CDS             complement(1761..1877)
                     /locus_tag="OM421_RS30595"
                     /old_locus_tag="OM421_30585"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_016947047.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     gene            2908..3099
                     /locus_tag="OM421_RS30600"
                     /old_locus_tag="OM421_30590"
     CDS             2908..3099
                     /locus_tag="OM421_RS30600"
                     /old_locus_tag="OM421_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_060568275.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLTLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3099..3476
                     /locus_tag="OM421_RS30605"
                     /old_locus_tag="OM421_30595"
     CDS             3099..3476
                     /locus_tag="OM421_RS30605"
                     /old_locus_tag="OM421_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_277696618.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFASVSVEQEEEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRP"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3504..4202)
                     /locus_tag="OM421_RS30610"
                     /old_locus_tag="OM421_30600"
     CDS             complement(3504..4202)
                     /locus_tag="OM421_RS30610"
                     /old_locus_tag="OM421_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     Typing          complement(2380..2860)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.86"
                     /coverage="91.0"
                     /evalue="7.770000000000004e-156"
ORIGIN
        1 tgtccgctgc cggacgggga tccgctgctc gctcctggcg gggcgcgagg gtcagcagtt
       61 catgggcgcg taatccggcg gccagggcaa tcgcggtggc cagagcgttg gcgggctgct
      121 gtgcagtggc aatcagggct acctggtccg ggctgtaggc ccggctgctc aggatctgtg
      181 cctgctccga tttcaccacc ggcaggtgtt cgccggcagg gagggtctgc gtcacgtgga
      241 tcatcatggc ctggatggcc tggcgttcca tatcgagcgt tttctggcca acctgctccc
      301 cgcgctgctc caggtaggtg atggcctgcg ccggtgtcag tgtgcgtaaa tcgccgtgat
      361 taatccggtt ggctttcatc cactctgcga cgcgggtcag tccctgctcg taattccgga
      421 ctgtgccgac agagcgcaga atatttccct ggagtggttt tatcacgcca gccgcctggc
      481 gggcagcgct ggcgaattta gccatttttt tccagccagc gggtaaccgt ggacaacgcc
      541 actttgattc tccgggcccg gagccagcgt tgcagctccg ctgcactggc cccggcctgg
      601 tacaggctca gcagttcgcc ggtatagcgg tcaaggcggg agggacggta gcggacctgg
      661 cggatacggc gggtctgcgc ctgcagggcg gccagttcgg cagcggtatc aaattgcatc
      721 tgtactccgg tgtgtaagaa catcagtgga aatccactgc ggcctgcgcc gatcgccctc
      781 gtttaaaccg taacgtggtt aagcccggac tgctcgcggc tcaacgtctc tttagtcaga
      841 gaatgacttg acccggttgt gggtctgaca gatttgggct gttgtctcag tgttctcgga
      901 ttgggctgcc cggtacagta tccccggagg gacccggctg ttttacctgc tgccggtagg
      961 caggtgcgcg tcagagtgcg cgggctcata gtttgactaa atactagcca ggtttttcgt
     1021 gcgtgtgaat gttgtttttt aaatgttgcg cctgactccg ctcaatagcg acccccgcta
     1081 tgtacaggcg catttatgcg tcgtacatag cgggtgtcgc aggtgctgat ttggcggaat
     1141 aaaaaacacc gggactgacc gccccggacc ccgccaacgg tgaccggaca gacaaaaaat
     1201 ttgcctgccc ggtcaccgtt tcacatcatg gcgaggaggc gggatctgaa acggtgtgat
     1261 gaaagtgcag atgaggtcag acgatgcagg tcggtgagtc cgtggtgaac gcgatagtgg
     1321 aataccacgc acagagcagt gaggaggcgc atgcagagga aaacaggatg ttcctgcgtt
     1381 atctgcacga gttaacggga acacagcgtg tggcggttga ggtggccagg aaatattttt
     1441 cgccggtgag ggctgatatg agctaccttt ttcttgtcgc cggcaccgca ccaggtgcga
     1501 cggtcctttt acagaattgt cctcatttga cgggcctgga tggcccgttc ttttatgctt
     1561 ttaacgtccg gggccctttt ctcggccgaa atcacctact atgcagtggt gtgcatagcc
     1621 atgcatatgc gtaaaataaa tcaggcgcgt ttctggcggt tttcggtgta cttgccacca
     1681 ccttttgccc gttaaccacc agaaacgccc tgaggccgtt ttcgcggtgc gcgtaagaag
     1741 ggattatggt aaatagggcg ctatcgccta cgcctaaatg tacgactgat tgtttttttt
     1801 tctctgaagt atgagattcc tgagtagaca gctacaacaa gtagagcaat ggcgatagca
     1861 agtgaaatcc agtccataaa tgcctgatta atttaataaa aacagcctgt tatgatttca
     1921 taaagataat atcatctttt tttagcatga tactgtcgtt acttattgtg gtgagatgaa
     1981 acataaccgc tgtttttgta tgtgtttgtt aaggctgcta agattattcc taaccactga
     2041 ttttgtacat atcatcaaaa aaataaaaaa actaatgccc tgtggtgggt atgtaacaaa
     2101 tcacggggag cgggtcagaa ctacgccaaa actttgaatg atcgggtggc aggctaaatg
     2161 aaatcttgag attcattcag cctcgtcgta atctcttgct ctgtaaacga aaaaaccgcc
     2221 ttgcagggcg gtttttcgaa ggttcagcaa gttggggaac ttctgaaccg tggtaacagg
     2281 gtgtacaaga ccgctgccac caaatgtgtc cttccagttt agccgtagtt aggcttcaac
     2341 ttcaagaact ctgtaccagt tatctcttgt acaccccttt accagtggct accgccaatg
     2401 gcgatttgtc gtgccttttc gggttggact caagacgata gttaccgaaa agagcgtggt
     2461 agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     2521 aactgagtgt caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta
     2581 aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa cgcctgggat
     2641 ctttatagtc ctgtcgggtt tcgccaccgc tgatttgagc gtcagatttt gtgatgcttg
     2701 tcaggggggc ggagcctatg gaaaaacggc aacgccgtgg ccttctcccg tctgttcttt
     2761 tccttttccg ggtggtggcg atgacttttc tgtctgtgtg ccccgtccgc tcgccgcagc
     2821 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaagagac tggtttgaca
     2881 ctgagcactg acgctctgag gccccttatg acgaaacaac agaaaactgc gctgaacatg
     2941 gctaaattca tccagaatca gtccctgctc ctgctggaga agcttaacga gcttgatctc
     3001 gatgcggagg ctgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca
     3061 ctgaccctca gactggatgc actacaggag ggtgactgat ggcgaacgtg tcgatcagtg
     3121 aggctgcacg cctgacgggt aagagcagga caaccctgca caggctaatt aaagccggtg
     3181 aactgtccac ctgtcggggt gaacggaatg cgaaaatgct cgatacttca gagcttttgc
     3241 gggtgtttgg tgcctttgcg tccgtatccg ttgaacagga ggaagaacag gtcagtggac
     3301 agcgtgacac agtcacttct gaccataatg aacaggtcat tcatactctg aaacaggaaa
     3361 ttgaacacct gaacacactt cttgcctcaa aagattcaca tatcgacagc ctgaaacagg
     3421 cgatgttgtt gctggagaac cgggaagatc agcggcagaa cacgacccgg ccctgatggc
     3481 gtttctggca gtcctgaaat gcctcagtgt ttttgccctg ctgttccgcc agtggcctgg
     3541 agtttttcca gtttccgtct gcgccgttcc gccgtcgcgc aggcatttct ccgtctttcc
     3601 tctgctttct ctgcttccag acgggccagc tcggtgttat gccgctcccc ggccacatat
     3661 ttccgggcct tctccagggt tgtttccagc gccagctcct ttttctgcag gatggattcg
     3721 cgctgattca gtgcctccct ctgttcttcc agctcctttc tggcttcttt ccgggcattc
     3781 tgcaccgcct gaagcaagag gctttctttt tgcgcaaacg cttcccgcag tacatcctca
     3841 gcaccaatgt agtggcttcg cagggccgct gtctccaggt tgtgattctg aataagggta
     3901 ttttgtctgg ctctgtgagc ctcttctgcc tccctcagtg cctgctgata tccgcttatc
     3961 tcgcgctgta tccggcggga ctctgagcct ctgaaaaaca aaatgaacag ccacagcgag
     4021 aacgctgtgg ctgcgatccc cgttgccagg atagcgggac cgagttcggt gaccgccttc
     4081 cagccagtaa acgcctcagg attgctgacg tccggatagt aaacgccata gtgattcagc
     4141 tcatacaggg cgtacgcgat ggcgaaaaca ccggtacaca ataaaataaa tgttcttgtc
     4201 atcgcaggta tgtctccgtg atttccggac gaaaatggcc catttcctgg ctgacggtgc
     4261 gcagggccat atcacgaacc agaccgagtt tttgcagctc ctgcatccgt tgctgggcgt
     4321 agctgtgacg cacgccgtgt gccccccgtg accagcccag ggtacgcatg gaggcggcac
     4381 tgaaggcgtt tgaccagcgc tggcctccgc caatggcgta ctgactctgg taaaacacgc
     4441 cgcgatcggt cacccggacc gggctggcca gcctgacatg ctcgagacgt tcagccaggc
     4501 gtgacggcag cagaacttcc ctgaccaggc cgcctttgcc ctgaacggtg taaatcacac
     4561 cctcccgacc ggcaaattta c
//
