LOCUS       NZ_CP110405             8401 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Shigella sonnei strain S17BD05200 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP110405
VERSION     NZ_CP110405.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28885477
            Assembly: GCF_026016185.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Berbers,B., Vanneste,K., Roosens,N.H.C.J., Marchal,K.,
            Ceyssens,P.-J. and De Keersmaecker,S.C.J.
  TITLE     Using a combination of short and long read sequencing to investigate
            the diversity in plasmid- and chromosomally encoded
            extended-spectrum-beta-lactamases (ESBL) in clinical Shigella and
            Salmonella isolates in Belgium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Berbers,B., Vanneste,K., Roosens,N.H.C.J., Marchal,K.,
            Ceyssens,P.-J. and De Keersmaecker,S.C.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2022) Transversal Activities in Applied Genomics,
            Sciensano, Juliette Wytsmanstraat 14, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 18:09:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,926
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,173
            CDSs (with protein)               :: 4,173
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 628
            CDSs (without protein)            :: 628
            Pseudo Genes (ambiguous residues) :: 0 of 628
            Pseudo Genes (frameshifted)       :: 169 of 628
            Pseudo Genes (incomplete)         :: 438 of 628
            Pseudo Genes (internal stop)      :: 108 of 628
            Pseudo Genes (multiple problems)  :: 85 of 628
            Pseudo Genes (short protein)      :: 3 of 628
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110405.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="S17BD05200"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2017-08-15"
     gene            join(7680..8401,1..115)
                     /locus_tag="OKF41_RS24425"
     CDS             join(7680..8401,1..115)
                     /locus_tag="OKF41_RS24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            421..663
                     /locus_tag="OKF41_RS24430"
     CDS             421..663
                     /locus_tag="OKF41_RS24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(695..1345)
                     /gene="tetR(A)"
                     /locus_tag="OKF41_RS24435"
     CDS             complement(695..1345)
                     /gene="tetR(A)"
                     /locus_tag="OKF41_RS24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1451..2650
                     /gene="tet(A)"
                     /locus_tag="OKF41_RS24440"
     ARG             1452..2647
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2617..2805)
                     /locus_tag="OKF41_RS24445"
                     /pseudo=""
     CDS             complement(<2617..2805)
                     /locus_tag="OKF41_RS24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2849..3238)
                     /locus_tag="OKF41_RS24450"
     CDS             complement(2849..3238)
                     /locus_tag="OKF41_RS24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3456..3896
                     /locus_tag="OKF41_RS24455"
     CDS             3456..3896
                     /locus_tag="OKF41_RS24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4663..4812)
                     /locus_tag="OKF41_RS24460"
     CDS             complement(4663..4812)
                     /locus_tag="OKF41_RS24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5383..5541
                     /locus_tag="OKF41_RS24665"
     CDS             5383..5541
                     /locus_tag="OKF41_RS24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            6001..6816
                     /gene="sul2"
                     /locus_tag="OKF41_RS24465"
     ARG             6002..6813
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6877..7680
                     /gene="aph(3'')-Ib"
                     /locus_tag="OKF41_RS24470"
     ARG             6878..7677
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     ARG             3..115
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.3"
                     /coverage="13.31"
     ARG             7681..8399
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="86.33"
     Typing          complement(4848..5021)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4945..5550
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
       61 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
      121 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
      181 agccttagcg taggattccg ccccttcccg caaacgaccc ctggaagccc cgtagcgacg
      241 cggagagggg cgagacaagc caagggcgca ggctcgatgc gcagcacgac atagccggtt
      301 ctcgcaagga cgagaatttc cctgcggtgc ccctcaagtg tcaatgaaag tttccaacgc
      361 gagccattcg cgagagcctt gagtccacgc tagatctatc tcatctgcgc aaggcagaac
      421 gtgaagacgg ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg
      481 acccgcgcca cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag
      541 gccgagcgcg gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc
      601 tgctacgggc gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg
      661 tagtgagtgg gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt
      721 caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct
      781 gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg
      841 gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct
      901 gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt
      961 ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct
     1021 gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg
     1081 cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg
     1141 gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc
     1201 gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt
     1261 tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca
     1321 ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc
     1381 caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg
     1441 gaggccagac gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga
     1501 cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca
     1561 ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt
     1621 tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct
     1681 cgtctcgctg gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg
     1741 ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg
     1801 cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag
     1861 cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt
     1921 ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg
     1981 ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct
     2041 caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc
     2101 ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg
     2161 cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat
     2221 tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag
     2281 gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc
     2341 gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat
     2401 gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca
     2461 aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc
     2521 gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct
     2581 ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agaaattctt caaattcccg
     2641 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
     2701 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
     2761 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
     2821 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
     2881 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccactctt acattgttcg
     2941 cagtatctag gtacatgtta ttgcgtgcac cttcatgatg tataatgtta ttaatgacag
     3001 cagaaagatc gacagaatta atattcttcg tacctgggac agttatcggg tgtcggccag
     3061 tgatgagacc aaaggatgca gtataagatg aaccactact attatcatgg ccaccattac
     3121 caccaccgtt aaaactacct cccgaacttc cagagccgct atgaccatca ttgtaggggt
     3181 catttggtcc catgtgacca aaaccaccga cagtaccgct atttaactct gtactcataa
     3241 aattatcctc tttgactttt aaaaacagca aaaactgtat ttataacaat gtgtttatat
     3301 acagtattcg aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt
     3361 ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt
     3421 acaatacggt acacacagga gacactgtca cttttatggc atggcacaca gttcaagaga
     3481 cactgaaatt aacaggaaag tcgcgctcgc aattatatcg tgacatggct aaaggccttg
     3541 tcagctatcg cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa ctgattcggg
     3601 cctatggcga gctgcgcact tctgagacac caaaaaggca caaggaggga cactccaaat
     3661 ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa ctggacgctc
     3721 tgcagcagac tgttacccta atgcttgaag acaagatggc gcgggaagca gaaaaccgcc
     3781 tgcgaaatga ggagagggaa caactacagg ccgaaattag ccgtctggct gacgcgctgg
     3841 cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg ctccggttca gagtaacagt
     3901 gcaggtgact ctgatttacc ataacatctg ttatcagcac cgcctgagag gccctcaggg
     3961 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
     4021 cgcaggctct gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattc
     4081 acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
     4141 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt
     4201 cagcacgttc aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag
     4261 ctgaaggaat gatgtaaaag tgatgtataa gtgatgtaaa agtgatgtat aagtgatgta
     4321 aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     4381 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     4441 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     4501 agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa aaaaggaata tttcgttttc
     4561 acgagtaata cccgggtatt aaaaatccac tgtaattaca tgaaaataaa gaaataaaag
     4621 gtaataccgg agtattacca cgtacgcggg caagcgaaaa agctataacg cttcccggcc
     4681 gacaggcgac ccaaaatcca caggggcatg caggttctct tcagtaacct gcgccattaa
     4741 atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc gaatccagtt gttcatcata
     4801 ttcagatggc atggggtgat atccagtagc tgagaggttg ttagcgggtt tcggggcgca
     4861 gccctgaacc agtcatgtag cgctagcgga gtgtatactg gcttactatg ttgccacgat
     4921 tgcgaaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcagcacc ggcgcgtcag
     4981 cagaatatgc gatacaggag atattccgct tcctcgctca ctgactcgct acgctcggtc
     5041 gttcggctgc ggcgagcggt accggcttac ggacggggac ggaaatttcc tggaagatac
     5101 caggaagaca cttagcaaag aagagataag gccacggcga agccgttttt ccataggctc
     5161 cgcccccctg acaagcatcg caaaatctga cgctcaaatc agtggtggcg aaacccgaca
     5221 ggactataaa gatcccaggc gtttccccct ggtagctccc tcgtgcgctc tcctgttcct
     5281 gcctttcggt ttaccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg
     5341 acactcagtt ccgtgaaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     5401 cagtccgacc gctgcgcctt gtccggtaac tatcaacttg agtccaaccc ggaaagacac
     5461 gacaaaacgc cactggcagc agccattagt aacaggagtg cacgagaaga tagattaaga
     5521 ggtcttgaag tagaggcctg actgcggcta cactgaaaga acagttttgg taatggcggt
     5581 ctcgtgaacc ctgttaccgg gttaagcagt tccccaactg acttaacctt cgaacaaacc
     5641 gcctccccag gcggtttttt cgtttacaga gcaagagatt acgattcatt gaaaaggatc
     5701 tcaagaagat cctttgtaga ttactatcaa ttaatcttcc gaacatgaca ccagtcactg
     5761 ttgattacgg ttaaaccgct ccgtaacagg tttccctcgt attggtgact tgcagcacca
     5821 aagaagcaaa aaagataaat atatctactt attgagagat gatatattta tttccaaaat
     5881 gctacagata catcatcatg aaacggatca ccgcaaacag gttactcgcc tggggattcc
     5941 ctttcgaccc gagcatccgt atgagactca tgctcgatta ttattattat agaagccccc
     6001 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
     6061 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
     6121 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
     6181 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
     6241 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
     6301 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
     6361 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
     6421 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
     6481 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
     6541 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
     6601 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
     6661 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
     6721 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
     6781 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
     6841 tattcgcgct tcatcagaaa actgaaggaa cctccattga atcgaactaa tatttttttt
     6901 ggtgaatcgc attctgactg gttgcctgtc agaggcggag aatctggtga ttttgttttt
     6961 cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc
     7021 gctggagagc gtgaccgcct catttggctc aaaggtcgag gtgtggcttg ccccgaggtc
     7081 atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat tccgggagta
     7141 ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa
     7201 cttggcgctg ttcacagcct atcggttgat caatgtcagt ttgagcgcag gctgtcgcga
     7261 atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg
     7321 gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg agagctaccg
     7381 gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc ctgcatgccg
     7441 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
     7501 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
     7561 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
     7621 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
     7681 tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg
     7741 acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct gcaatcgtca
     7801 agggattgaa acctatagaa gacattgctg atgaactgcg cggggccgac tatctggtat
     7861 ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg atgttgctcg
     7921 aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac taccaggcga
     7981 ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa cccctgcctt
     8041 ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc
     8101 aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat caaatgatga
     8161 gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac atcatgttct
     8221 ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg ggctttggcg
     8281 ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat cctagacgca
     8341 ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg
     8401 a
//
