LOCUS       NZ_CP110399             8401 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Shigella sonnei strain S17BD06357 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP110399
VERSION     NZ_CP110399.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28885482
            Assembly: GCF_026016165.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Berbers,B., Vanneste,K., Roosens,N.H.C.J., Marchal,K.,
            Ceyssens,P.-J. and De Keersmaecker,S.C.J.
  TITLE     Using a combination of short and long read sequencing to investigate
            the diversity in plasmid- and chromosomally encoded
            extended-spectrum-beta-lactamases (ESBL) in clinical Shigella and
            Salmonella isolates in Belgium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Berbers,B., Vanneste,K., Roosens,N.H.C.J., Marchal,K.,
            Ceyssens,P.-J. and De Keersmaecker,S.C.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2022) Transversal Activities in Applied Genomics,
            Sciensano, Juliette Wytsmanstraat 14, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Partial
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 17:11:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,823
            Genes (coding)                    :: 4,205
            CDSs (with protein)               :: 4,205
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 618
            CDSs (without protein)            :: 618
            Pseudo Genes (ambiguous residues) :: 0 of 618
            Pseudo Genes (frameshifted)       :: 161 of 618
            Pseudo Genes (incomplete)         :: 433 of 618
            Pseudo Genes (internal stop)      :: 108 of 618
            Pseudo Genes (multiple problems)  :: 82 of 618
            Pseudo Genes (short protein)      :: 3 of 618
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110399.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="S17BD06357"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2017-09-14"
     gene            323..481
                     /locus_tag="OKF40_RS24795"
     CDS             323..481
                     /locus_tag="OKF40_RS24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            941..1756
                     /gene="sul2"
                     /locus_tag="OKF40_RS24555"
     ARG             942..1753
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1817..2620
                     /gene="aph(3'')-Ib"
                     /locus_tag="OKF40_RS24560"
     ARG             1818..2617
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            2620..3456
                     /locus_tag="OKF40_RS24565"
     CDS             2620..3456
                     /locus_tag="OKF40_RS24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3762..4004
                     /locus_tag="OKF40_RS24570"
     CDS             3762..4004
                     /locus_tag="OKF40_RS24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4036..4686)
                     /gene="tetR(A)"
                     /locus_tag="OKF40_RS24575"
     CDS             complement(4036..4686)
                     /gene="tetR(A)"
                     /locus_tag="OKF40_RS24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4792..5991
                     /gene="tet(A)"
                     /locus_tag="OKF40_RS24580"
     ARG             4793..5988
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<5958..6146)
                     /locus_tag="OKF40_RS24585"
                     /pseudo=""
     CDS             complement(<5958..6146)
                     /locus_tag="OKF40_RS24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(6190..6579)
                     /locus_tag="OKF40_RS24590"
     CDS             complement(6190..6579)
                     /locus_tag="OKF40_RS24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6797..7237
                     /locus_tag="OKF40_RS24595"
     CDS             6797..7237
                     /locus_tag="OKF40_RS24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(8004..8153)
                     /locus_tag="OKF40_RS24600"
     CDS             complement(8004..8153)
                     /locus_tag="OKF40_RS24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2621..3453
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(8189..8362)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          6..490
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.854"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 accggcttac ggacggggac ggaaatttcc tggaagatac caggaagaca cttagcaaag
       61 aagagataag gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatcg
      121 caaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc
      181 gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
      241 cattccgctg ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgtgaaggc
      301 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
      361 gtccggtaac tatcaacttg agtccaaccc ggaaagacac gacaaaacgc cactggcagc
      421 agccattagt aacaggagtg cacgagaaga tagattaaga ggtcttgaag tagaggcctg
      481 actgcggcta cactgaaaga acagttttgg taatggcggt ctcgtgaacc ctgttaccgg
      541 gttaagcagt tccccaactg acttaacctt cgaacaaacc gcctccccag gcggtttttt
      601 cgtttacaga gcaagagatt acgattcatt gaaaaggatc tcaagaagat cctttgtaga
      661 ttactatcaa ttaatcttcc gaacatgaca ccagtcactg ttgattacgg ttaaaccgct
      721 ccgtaacagg tttccctcgt attggtgact tgcagcacca aagaagcaaa aaagataaat
      781 atatctactt attgagagat gatatattta tttccaaaat gctacagata catcatcatg
      841 aaacggatca ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt
      901 atgagactca tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat
      961 tttcggcatc gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc
     1021 agacgcagcc attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct
     1081 cggtccggca tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg
     1141 tatcgcgccg gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta
     1201 tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg
     1261 cggttttcca gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt
     1321 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
     1381 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
     1441 acgcaaccgc cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac
     1501 ctcgctctcg gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct
     1561 tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt
     1621 cggggccgcg acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg
     1681 cacacacgag ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac
     1741 cgcaagaatt cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa
     1801 actgaaggaa cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg
     1861 gttgcctgtc agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc
     1921 cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct
     1981 catttggctc aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca
     2041 ggagggtgca tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg
     2101 agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct
     2161 atcggttgat caatgtcagt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga
     2221 tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc
     2281 gcagctcgat cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg
     2341 caccgatatg gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa
     2401 aactcttcaa tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc
     2461 cgatttggca ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga
     2521 gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct
     2581 tgccttctat ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt
     2641 cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt
     2701 tggaaagttt cattgccaga cgggactcct gcaatcgtca agggattgaa acctatagaa
     2761 gacattgctg atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca
     2821 gtccggttgc tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg
     2881 ctctctcaca tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta
     2941 atggcgaagc tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg
     3001 gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact
     3061 gactacgtcc acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt
     3121 gggctacatg gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg
     3181 atagatcccg tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat
     3241 ccggctgaca gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca
     3301 ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg
     3361 tgcctttccg cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg
     3421 gccgcgatca agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc
     3481 ctgggaacac atcaatctca ccggagaata tcgctggcca aagccttagc gtaggattcc
     3541 gccccttccc gcaaacgacc cctggaagcc ccgtagcgac gcggagaggg gcgagacaag
     3601 ccaagggcgc aggctcgatg cgcagcacga catagccggt tctcgcaagg acgagaattt
     3661 ccctgcggtg cccctcaagt gtcaatgaaa gtttccaacg cgagccattc gcgagagcct
     3721 tgagtccacg ctagatctat ctcatctgcg caaggcagaa cgtgaagacg gccgccctgg
     3781 acctcgcccg cgagcgccag gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg
     3841 agcggacgcc gcagcaggag cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg
     3901 cttggacgct agggcagggc atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc
     3961 ggtggaaagg gggaggggat gttgtctaca tggctctgct gtagtgagtg ggttgcgctc
     4021 cggcagcggt cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag
     4081 cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg
     4141 accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc
     4201 aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc
     4261 aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc
     4321 ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt
     4381 gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc
     4441 attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt
     4501 ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca
     4561 gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc
     4621 tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt
     4681 tgtcatgctt gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata
     4741 aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc
     4801 aacagacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg
     4861 attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc
     4921 cactatggca ttctgctggc gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg
     4981 ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct
     5041 gctgtcgact acgccatcat ggcgacggcg cctttccttt gggttctcta tatcgggcgg
     5101 atcgtggccg gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc
     5161 actgatggcg atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg
     5221 atggtcgcgg gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc
     5281 ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag
     5341 tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc
     5401 cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa
     5461 cttgtcggac aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg
     5521 gacgcgacca cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag
     5581 gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga
     5641 atgattgccg acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg
     5701 ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg
     5761 ttgtccaggc aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc
     5821 accagcctga cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata
     5881 acaacgtgga acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg
     5941 gcgctgcgtc gcgggctttg gagaaattct tcaaattccc gttgcacata gcccggcaat
     6001 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     6061 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     6121 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     6181 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     6241 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     6301 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     6361 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     6421 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     6481 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     6541 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     6601 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     6661 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     6721 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     6781 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     6841 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     6901 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     6961 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     7021 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     7081 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     7141 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     7201 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     7261 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     7321 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     7381 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     7441 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     7501 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     7561 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     7621 gtgatgtata agtgatgtaa aagtgatgta taagtgatgt aaaagtgatg tatgaactga
     7681 aggagcgatg taattctgta gtcaggaaga ataacccgtt catgcaggca tgaacgatac
     7741 agtgaggaac gctttttttg tcacgatctc cccttaacct gcgtaaatca cccgccatgg
     7801 ttgattattt ttcaacctca aaatggtcat acacctaacg cagccgcgat tttcatcgca
     7861 actgcatgaa ttaaaagaaa aaaaaggaat atttcgtttt cacgagtaat acccgggtat
     7921 taaaaatcca ctgtaattac atgaaaataa agaaataaaa ggtaataccg gagtattacc
     7981 acgtacgcgg gcaagcgaaa aagctataac gcttcccggc cgacaggcga cccaaaatcc
     8041 acaggggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg
     8101 ccgggaagct gcgtatcagt cgaatccagt tgttcatcat attcagatgg catggggtga
     8161 tatccagtag ctgagaggtt gttagcgggt ttcggggcgc agccctgaac cagtcatgta
     8221 gcgctagcgg agtgtatact ggcttactat gttgccacga ttgcgaaaat cagtgaagtg
     8281 cttcatgtgg caggagaaaa aaggcagcac cggcgcgtca gcagaatatg cgatacagga
     8341 gatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg
     8401 t
//
