LOCUS       NZ_CP110293             5207 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Enterococcus faecalis strain AKSZ-208 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP110293
VERSION     NZ_CP110293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN27545017
            Assembly: GCF_023182775.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Xuan,H.
  TITLE     Various mobile genetic elements carrying optrA in Enterococcus
            faecium and Enterococcus faecalis isolates from swine within the
            same farm
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Xuan,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) Shanghai Veterinary Research Institute,
            Chinese Academy of Agricultural Science, No. 518, Ziyue Road,
            Shanghai 200241, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v. 2.4.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 17:13:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,959
            Genes (coding)                    :: 2,852
            CDSs (with protein)               :: 2,852
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 35 of 107
            Pseudo Genes (incomplete)         :: 61 of 107
            Pseudo Genes (internal stop)      :: 28 of 107
            Pseudo Genes (multiple problems)  :: 16 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="AKSZ-208"
                     /host="environment"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Xinjiang"
                     /collection_date="2018-10"
     gene            join(4531..5207,1..1003)
                     /gene="mobV"
                     /locus_tag="MZO28_RS15115"
                     /old_locus_tag="MZO28_15115"
     CDS             join(4531..5207,1..1003)
                     /gene="mobV"
                     /locus_tag="MZO28_RS15115"
                     /old_locus_tag="MZO28_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_104659816.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKEIQVSKQELANQEQILVEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANIKEKKFSFNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDRTTQKVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            1092..1292
                     /locus_tag="MZO28_RS15120"
                     /old_locus_tag="MZO28_15120"
     CDS             1092..1292
                     /locus_tag="MZO28_RS15120"
                     /old_locus_tag="MZO28_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            2597..3328
                     /locus_tag="MZO28_RS15125"
                     /old_locus_tag="MZO28_15125"
     CDS             2597..3328
                     /locus_tag="MZO28_RS15125"
                     /old_locus_tag="MZO28_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_025188181.1"
                     /translation="MNEKSELAVKYQNELNLVALKQFNAKEMNLFFALCARMKDKGLDS
                     IIFSFEELKELSDYKMTAIKAFTNDLDSLYSKMLQLTYRNEYDDDGSFRRFVLFTGFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSRFELSAFTAIRSTYAKTLFRLLMQYRSTGYYT
                     VSIDEFKEIMDIPKSYTQMGQIDQRVIKPAMKELNNYFENLELTKIKAKKGNKIAKLEF
                     TFTGLKTNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     gene            3456..3752
                     /locus_tag="MZO28_RS15130"
                     /old_locus_tag="MZO28_15130"
     CDS             3456..3752
                     /locus_tag="MZO28_RS15130"
                     /old_locus_tag="MZO28_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002404941.1"
                     /translation="MGLMTGYNMTKEEYEITQAVLTRLDKFESELESLLLDLEPLDLSR
                     LDTVVREETLRMFNYLSRSYILLLDTVAYKDTLEEDISSGIVKIDGQPLDEEE"
                     /name="hypothetical protein"
     Typing          3..1000
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.598"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4457..5205
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="99.0"
                     /evalue="2.91e-159"
ORIGIN
        1 tattgaccgt cttcttgaag agaagaacga agaagtaaag ggcaaagtcc aagccatgaa
       61 ccaactggaa acgaagcgaa acgaaatcat taactctcta caaacaaccg ttttacctga
      121 tttagcaaat atcaaagaaa agaaatttag ttttaacggt ttaaaatacg ttctaagcgc
      181 ttctgacctt tcggcaataa aaagtattac gaaagatttt gaaacgctta gaacgcaaaa
      241 taagcacctt aaaatcgaaa acaatacctt ggcgcagaaa aataaagaat tgacacaagt
      301 aaatcatgaa caagaattac aaataacgga cttagcctat gaattaaccc tgaaaaaaga
      361 acaattaaca gcgtttgaag aaaaacaact aaactatgac cagagcgaac aaatcaaaca
      421 agaacttgat agaacaacac aaaaagtttc tgtgttagaa acagagaata agcggttaaa
      481 agataaaatt actgacttaa cctacgactt ttctgaacta aacgaaaaat atcaagagat
      541 aaaactaaag tttgacgggt taaccactta ccttcatgag agactaggac aggccaaaga
      601 aaccatttta tttaccattc agcaaggagt gaagtacctt cagaacgcgc ctaaattaaa
      661 aaaatccctg gagcgatatt ccaaggacag aacctacctc attgacgaaa aagggacatt
      721 gtttttacca ccagatacgg ggtcaaaagg ggtaacaatg tccttaaacc cacatttcta
      781 ccccctaaaa gccccgtttt acaacagaga aacagggtgt tatgagtttg tcggcgagta
      841 tatggcaaat ggccgttcac gaactaatac tttacagctg tctgaagaac aagtgctaga
      901 gaagaaagct tttacgtttg aacagtcgac agataaaaag gcttggcaac agagtaaaaa
      961 atattcaaga gatatgtcaa aaggacgagg tttatctcgt taaaaataat ttaattgata
     1021 aattattgac acataaaaac agttaaatta aaatatatta ataaaaatta aaaaaaagtg
     1081 atgtaatcat tatgaaaaag ttgctagttc taattttagg tgtgtctttt atctctgttg
     1141 ctttaagttc aactacagaa gcaagtgcta gagagtggga ttctatagag tgtcagccag
     1201 gattttattg gaaaaaaggc gggtggactg caggaggcta tcataaagcc ggtggatttt
     1261 gtatcccaat gcatacaaga tggacaaaat agttgctaaa tttgaggtta ttttatgaaa
     1321 aaatatctaa taaaaaatat attttatatt actattcctt tagttttaag ctatataact
     1381 tcttttttag ttaatattga tttgcccata ttgataatta ttttttacgg aattttatta
     1441 ttttttttaa ttccttcaga agtatatttg ggaagtacaa tggattacaa cgcgaaagta
     1501 gtgaatccca cttacagacc agaaaataaa agctttgaag atagtccaaa aagtaaaata
     1561 ttgtctatcc tcatagtatt actatgttta atactaacta tttcgatatg gtatttctct
     1621 aattgacgtt ttgcgtaatc taatctgacg tttgaaaata cagaaaaaaa atgttattgt
     1681 gtaagtgcga agcgaaatta agcaaaatca tgtttacacc aaaaagccct cctattctca
     1741 gtaggagggc tttttttaca ctgttgcgat ttcagcgttg gagctagaaa agcaactgca
     1801 tagattatct gctaatcgtc tagccaacga tcaactagca aaagtactaa acccacaaaa
     1861 agtggggcga taatcaacga aattaagctt tcaatcatgt ttacaccccc tttcggggca
     1921 gttgccaaaa caagtatatc acaagaagaa tttcttgtgg gggttgttag ggcttgccct
     1981 gaccgcctgc aaggcgcaat cttaacaaga ttacctgaaa ccactaaaca cgtcttaaag
     2041 agcttcagac aagcaaatag agcttaacca aatgagttac cttgtcaacg gtattgattt
     2101 tgattttagc gagtgaacga agtgaacgct gcaagcataa tgtgagcgaa gaatttcgct
     2161 cactcctttt tttgtttttc aattttttag tgacagtttc gtaaatatcg cgaacctgtt
     2221 tgtagaaaaa agagtgaatt tttaggcggg aaatttcctc gcacgcgcac gcgtttattt
     2281 ttatatattt tttatatatt ttttaaaacc tttttaggcg gtctgtgtgc cacgtggctc
     2341 taaggaagag cagagggtac caacatcatt ttatctgtgt accaacatca ttttatctgt
     2401 gtaccaacat cattttatct gtgtaccaac atcattttat ctgtgtacca acatcatttt
     2461 atctgtgtac caacatcatt ttatctgact actaataaca aattatcttt gtaaaatcac
     2521 gctcacatta tataaacagc atacttgaca ttaatgtatg ctgtttatat aatgtgagcg
     2581 tgagggggcg aaaggaatga atgaaaaatc ggaattagct gttaaatatc aaaacgaatt
     2641 aaatctagtt gctttaaaac agttcaatgc taaagaaatg aacttatttt ttgccttatg
     2701 tgcaagaatg aaagacaaag gtttagacag tatcatattt tcttttgaag agttaaaaga
     2761 attaagcgac tataaaatga ctgcaattaa ggcgtttaca aatgatttag atagcctata
     2821 cagtaaaatg ctgcaactaa cttatcggaa tgagtatgat gatgacggaa gttttcgacg
     2881 atttgtcttg tttactggct ttgacgttaa tgtaaaaaaa caaacagttg aagtaagtat
     2941 caatcctaaa ttggaaggta ttcttaatgg cttaacaact gaatttagta ggttcgagtt
     3001 atctgcattt actgctatcc gatcaaccta tgctaaaacg ttgtttcgct tattaatgca
     3061 atatcggtca actggttact ataccgttag tattgatgaa tttaaagaaa taatggatat
     3121 tccaaaatcg tatactcaaa tgggacaaat agaccaaaga gtgataaaac ctgcaatgaa
     3181 agaactcaat aattactttg agaatttaga attaacaaaa atcaaagcca aaaaaggcaa
     3241 taagattgcc aaactagaat ttacgtttac tggtctgaaa accaacaaac caagcataac
     3301 tatgcatgac tgggtaaacg gagaataaaa aaagaacttg ccccctcaca ttaagcaagt
     3361 tagcaagttc ctcacgcaat ccactacgac tgctagatta ttttagcata tttgcgtgag
     3421 gacttctaaa ataaattatt ttggaggtca tatacatggg attaatgaca ggatacaaca
     3481 tgacaaaaga ggagtacgaa atcacacagg ctgtactgac tcgactagat aagtttgaaa
     3541 gtgaactaga atcattatta cttgatttag agccactcga cctatcacga ttagatactg
     3601 tcgtccgaga agaaacgcta agaatgttta attatctaag tcggtcgtac attctacttc
     3661 ttgatacggt cgcctacaaa gatacgctag aagaagatat ttccagtggg atcgtgaaaa
     3721 ttgacggtca gcctctagac gaagaagaat aaagactagg tctgattgtt ataaaaaagt
     3781 attagatatt cggggtacac tcgaaaaata tcaagattag tctacttcca ttttggggta
     3841 ggctttcttt ttgcaagcag agcaacatgg taccatgttg cgatattttg aatgacacct
     3901 accagtattt ttggtaggtg tcattcaaat ttttggggtt cccccaatcc ccactgttgt
     3961 tattttttgt cttttgttct tgaaatttac ttaattgttt catgtgtttc ggaagagcat
     4021 aagggaaact gggtcccaca caaggtggga cgccttgcag tgcaaggctt tcccgtgggg
     4081 tacactttcg ttcttcgcta gcactttact agcaggtact ttcaggtaaa ctagcacatt
     4141 tttagcactc actagcaggt ttctagcacc caaactagca gattcctagc agtgactagc
     4201 aggtttttag caccaaatta gcacattact agcaccggct agcagaaagc tagcacgttc
     4261 actaatttca ggcagtcgtt atttaaacgg ttttttgacg gaggggaatt tggagtttct
     4321 gccttagcag aaatggaaag tttgaccgta ggcaatcggc taagtgcgag attgggaagc
     4381 ctaaaggctg accttccgag tactagccga cagccctcgg cagagcgcac ctttacgaag
     4441 taaagtatag tgcgttatac tttacttgga agtgctgccg aaatccaaag acaaagaacg
     4501 ccacaagaaa aaaaaggggg gggagcgctc atgtcaaaaa ttgtcgcacg tatggagaaa
     4561 atgaaagttg gaaacttaag cggtatccaa cggcataatc aacgagaaac cgataatcat
     4621 tccaatcctg atattgatat tgagaaatct cacttgaatt atgacttagt caatcctggt
     4681 tcaatcaatt atcgggagaa aatcaaacaa atcattgaga gccaacgaat cagtaaacga
     4741 gcggttagaa aagacgcagt ccttgtgaac gaatggataa tcactagtga taccgccttt
     4801 ttccaagaga atacagacac acaagcattt tttaccgatg ttgtcgcgta tttctctgat
     4861 cgctgcggtc gacaaaatgt cgcctatgcc acggtacatt tagacgaaac cacgccccat
     4921 atgcacttag gaattgtgcc tatgtacgaa gggcgattga gcagtaaaca ggtgtttagt
     4981 cggcaaagtt tgttagagat tcaggaagaa ctgccaaact atttgaaaga acggggctac
     5041 gctattgagc gtggactccg tgggagtccg caaaagcatt tatctgtcaa agaatacaaa
     5101 gacgttcaaa aagaaataca ggtttctaaa caagaactcg cgaatcagga acaaattctg
     5161 gtagaaaaag aaagagaact ccattcctat gaacaggcaa tcttccg
//
