LOCUS       NZ_CP110077            13461 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Enterococcus faecalis strain BE5 plasmid pBE5_3, complete sequence.
ACCESSION   NZ_CP110077
VERSION     NZ_CP110077.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31342455
            Assembly: GCF_025958925.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13461)
  AUTHORS   Gaeta,C., Pinzauti,D., Marruganti,C., Bollini,R., Fabbro,A.,
            Santoro,F., Grandini,S. and Pozzi,G.
  TITLE     The presence of Enterococcus faecalis in saliva as a risk factor for
            endodontic infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13461)
  AUTHORS   Pinzauti,D. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2022) Dipartimento di Biotecnologie Mediche,
            University of Siena, viale Bracci 16, Siena 53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85x
            Sequencing Technology :: Oxford Nanopore GridION, Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 05:18:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,010
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,837
            CDSs (with protein)               :: 2,837
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 47 of 96
            Pseudo Genes (incomplete)         :: 42 of 96
            Pseudo Genes (internal stop)      :: 28 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110077.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13461
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BE5"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pBE5_3"
                     /geo_loc_name="Italy: Tuscany, Siena"
                     /collection_date="2019-01-28"
     gene            complement(join(10656..13461,1..272))
                     /locus_tag="OLM06_RS14945"
                     /old_locus_tag="OLM06_14925"
     CDS             complement(join(10656..13461,1..272))
                     /locus_tag="OLM06_RS14945"
                     /old_locus_tag="OLM06_14925"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138440.1"
                     /GO_component="GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA]"
                     /GO_function="GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="WP_142666703.1"
                     /translation="MSHSEQMIENQFIQILSEKENQWTYRPDLKSEEALWQNFRGHLNR
                     INLAVLEEQLLTDKEFKQVKVEFSRLTATPFLASQWLRGENGVAQVLLEREDGKKVTLE
                     AFRNKDISGGTSSYEVVHQVVPDSSRVDRGDVSLLINGLPIIHIELKKESAKDGFMQAY
                     YQIQRYAEDGFFKGIYATTQIMVISNKVDTRYFARPSEDTAEAYARMKKFLFNWRTEDN
                     QTVSDLFDFTRTVLRIPDAHELISQYTILVDDQKNQKFLMALRPYQIHAIRKIRKKATQ
                     HEGGFIWHATGSGKTITSFVATKLLAQNAIGVDRTVMVVDRTDLDAQTQDEFTKFASEY
                     HTGQTTGNSVANTLIVGIKNQKQLARNLLSSKNNNTILVTTIQKLSAAMRSAQQESEEK
                     GSNQFEKLRQEHLVFIVDEAHRAVSDEEMKRIKKILPNSTWFGLTGTPIFEENKKQENG
                     TFARTTSQQYGPLLHSYTTKNAMDDGAVLGFQVEYHSLISEEDQEVIVTQLNKGKLPDD
                     ALQQEKLLPTELYETDEHIRTMLQKIFNRRSVVKKFKVKNGFPTMSAILTTHSIAQAKH
                     IYRILKEMKDNGTLLNGRQFDERHQLIDKDFPRVAITFSTNPDQLEKNEQDDELVEIMK
                     EYAKQFDASPYQDEKLYNQNINKRLARKEKQYQSDGQWLDFVIVVDRLLTGFDSPTIQT
                     LYIDREMNYQKLLQAFSRTNRIYTGKDSGLIVSFRKPFTMKENVQNTFRLFSNEKQNFD
                     QLIPREYEEVKKEFIECSTLYKQSEAVLSDNPNDLKTMIAQVSAYQKLEKSYKALRSYD
                     QYEEDFEAFSEVVEQLPQYQGKTENIKTKIKEMIEDEGHPEEDFEKLLQEIAFSSQLNA
                     THKDVVDSFYINQLLKAIQLNEAGAVEKFEKEIQQKDPQIQKMYHTLKDQLVNTAEEID
                     VAQLKETSIQNEIQRQLQKEAEEFGLSFDFLQSAMNEYQGDKKTIPYLTHLLDSMTLSK
                     EEFEAKTGEKYRRRTKVLEERLQQNFEHLQKWKEEL"
                     /name="HsdR family type I site-specific deoxyribonuclease"
     gene            complement(284..874)
                     /locus_tag="OLM06_RS14950"
                     /old_locus_tag="OLM06_14930"
     CDS             complement(284..874)
                     /locus_tag="OLM06_RS14950"
                     /old_locus_tag="OLM06_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB-like protein"
                     /protein_id="WP_073340479.1"
                     /translation="MSENLKTIKEIADEIGVSKQAVWQKIKKESSIDLRQFTSKKGNTV
                     YVDVDGQKVIKNAFFNKTSTKKRQQKVFVDDNVNNSVDGNPEGNEEILFLRNLVSELQS
                     EKKELHKLLDQQQRLALQDKKLLEEYKSEINELKALKMPQEDMKDGSSIRGEAQEEIER
                     LKAQLKSSEEERNKAKEKEPVKTESKKWWQLWK"
                     /name="RepB-like protein"
     gene            complement(867..2057)
                     /locus_tag="OLM06_RS14955"
                     /old_locus_tag="OLM06_14935"
     CDS             complement(867..2057)
                     /locus_tag="OLM06_RS14955"
                     /old_locus_tag="OLM06_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876136.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_085407407.1"
                     /translation="MSIISENQKQVYTLNEIEKRKVVEHNSLITSIAKMQKTALKMFEL
                     AVSCIDTENPPKDNIIYLSKKELFSFFDVSSASKHTRFKEAIEYMQKQAFFQIKEEKNK
                     GYEMTSIVPIPTVKWNSYNDDVMIQFNQFIMPYLIDLKAEFTQYKISELKELNSKYSLI
                     LYRWLAMNYSQYEHYSVKGGRRAEQVEAYRNPSITVKELREITDTINEYTRFQSFENRV
                     LKNAIEEINAHTSFNVSYEKVKKGRSIDSIVFHIEKKRRADDNSYKLEDTAFQVDKKQK
                     EQSEAVLVKQAMESKYTRLLLDNMLLSPYEMTDTSLMAGLQAHVYPLYDELKALRGLNG
                     VKDHLSYVRAKQEAYSKRNIAKYLKKAIEQYLPTVKLQDLEQPERASVRGKGEIHE"
                     /name="RepB family plasmid replication initiator protein"
     gene            2957..3211
                     /locus_tag="OLM06_RS14960"
                     /old_locus_tag="OLM06_14940"
     CDS             2957..3211
                     /locus_tag="OLM06_RS14960"
                     /old_locus_tag="OLM06_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epsilon-antitoxin"
                     /protein_id="WP_010824143.1"
                     /translation="MKLDYRKTFEIEIINEFQSAIHSKMLNFVLNNEFDKSDSKNLQTN
                     LLNQLSNMNQINLFKLSLEELEAYHEYLRAIKKYADSIT"
                     /name="epsilon-antitoxin"
     gene            3311..3739
                     /locus_tag="OLM06_RS14965"
                     /old_locus_tag="OLM06_14945"
     CDS             3311..3739
                     /locus_tag="OLM06_RS14965"
                     /old_locus_tag="OLM06_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005917942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_002396183.1"
                     /translation="MSNEIQKRNSDTYNMTPFNFFEDFSRNLFNDFKPNLIKTDIHETD
                     NEYFVEAELPGIPKEDIQVTYENGVLTISGQHQIDTATEDKKGRLIRSERSLTSVRRQY
                     LLENVKEDEIKASYSEGILKLTLPKDSNKEIKKSIPIE"
                     /name="Hsp20/alpha crystallin family protein"
     gene            complement(4165..4290)
                     /locus_tag="OLM06_RS14970"
                     /old_locus_tag="OLM06_14950"
     CDS             complement(4165..4290)
                     /locus_tag="OLM06_RS14970"
                     /old_locus_tag="OLM06_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_229236143.1"
                     /translation="MSRSHLNYDLVDRTYNYKTDIERYINENKSSPRAIRNKDEL"
                     /name="plasmid recombination protein"
     gene            4787..5149
                     /locus_tag="OLM06_RS14975"
                     /old_locus_tag="OLM06_14955"
     CDS             4787..5149
                     /locus_tag="OLM06_RS14975"
                     /old_locus_tag="OLM06_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_002396185.1"
                     /translation="MEKVCQVAIVHKEKVNVIKNKLDQKDFSNLLVLGKCFSDSSRIKI
                     FYALETYKEMCVCDLAEILNASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIHIFLKLSENLSMKS"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5166..5783
                     /locus_tag="OLM06_RS14980"
                     /old_locus_tag="OLM06_14960"
     CDS             5166..5783
                     /locus_tag="OLM06_RS14980"
                     /old_locus_tag="OLM06_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="WP_002319351.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGLRQIFWGQYL
                     GTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVALVGEEEEEEEEVVEKLESR
                     GTNRFFWTVALITIASGGDNLGIYIPYFASLSFSEIVTALIVFAISVAVLCYISYKLAK
                     ISFVSETLEKYERILVPIVFIGLGIYILIENGTIQTVLSLFN"
                     /name="CadD family cadmium resistance transporter"
     gene            6024..6575
                     /locus_tag="OLM06_RS14985"
                     /old_locus_tag="OLM06_14965"
     CDS             6024..6575
                     /locus_tag="OLM06_RS14985"
                     /old_locus_tag="OLM06_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_002396187.1"
                     /translation="MSYNVQPLRTQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRMS
                     DIVKLKKKDVISSKNPRIVEQKTGKTRILYLSSLQELIQDYTKDLEPEDYLFPSTKGGH
                     VEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKITKR
                     YIGINEDEISETLLNFRLGF"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(6745..6975)
                     /locus_tag="OLM06_RS14990"
                     /old_locus_tag="OLM06_14970"
     CDS             complement(6745..6975)
                     /locus_tag="OLM06_RS14990"
                     /old_locus_tag="OLM06_14970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264563395.1"
                     /translation="MRTYTPLQNWVVFVLKKMYQKSASRQQILETPENDPKRSFRPKQN
                     RNRENSTRNEPEQPPIKRDQSDHNRDHGPSL"
                     /name="hypothetical protein"
     gene            complement(<7471..7560)
                     /locus_tag="OLM06_RS14995"
                     /old_locus_tag="OLM06_14975"
                     /pseudo=""
     CDS             complement(<7471..7560)
                     /locus_tag="OLM06_RS14995"
                     /old_locus_tag="OLM06_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /name="DNA recombinase"
     gene            complement(7899..9068)
                     /locus_tag="OLM06_RS15000"
                     /old_locus_tag="OLM06_14980"
     CDS             complement(7899..9068)
                     /locus_tag="OLM06_RS15000"
                     /old_locus_tag="OLM06_14980"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256082.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_142666701.1"
                     /translation="MSKKSPQLRFKGFTDDWEERKLGEVGYFYYGKSAPKWSVTEDAET
                     PCVRYGELYTKYGAKISNIYSHTNINPKNLKFSTGNEVLVPRVGENPLDFSLCSWLSIP
                     NVAIGEMISVYNTNENPLFISYYFRSQMKYEFAKRVEGGNVSNLYYSYLEDIDLYIPKL
                     EEQIAISKFFEKLDDTIALHQRKLDLLKEQKKGYLQKMFPKNGAKVPELRFAGFADDWE
                     ERKLKDVVEKQIKGKAQFEKLAQGDVEYLDTSRLNGGQAILTNGLKDVTLDDILILWDG
                     SKAGTVYHGFEGALGSTLKAYRTSANSKFVYQYLKRHQDNIYNNYRTPNIPHVQKDFLN
                     VFTISVPGSDEQAKIGSFFKKLDDTIALHQRKLDLLKEQKKGFLQKMFV"
                     /name="restriction endonuclease subunit S"
     gene            complement(9061..10656)
                     /locus_tag="OLM06_RS15005"
                     /old_locus_tag="OLM06_14985"
     CDS             complement(9061..10656)
                     /locus_tag="OLM06_RS15005"
                     /old_locus_tag="OLM06_14985"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342416.1"
                     /GO_function="GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA]; GO:0009007 -
                     site-specific DNA-methyltransferase (adenine-specific)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA];
                     GO:0009307 - DNA restriction-modification system [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /protein_id="WP_142666702.1"
                     /translation="MATGLNQQLWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLRE
                     VYEQENGKTDTFPERSTQYAGFMEWYEEDKDDLIENIQPKQGYFIQPDQLFYHYRIKAD
                     NYEFNLTDLQAGFNELERQGEEFRGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEID
                     LFEHNGDVIGDAYEYLIGMFAAGAGKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYD
                     PAMGSGSLMLNIRRYLKNPNQVHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTL
                     DADWPSEEPYQFDSVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSKADFAFLLHGFY
                     HLKESGTMGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIIL
                     KKNRSRRDVLFIDASQDFEKQKNQNVLLDEHIDKIVSIYKKREDIERYAHVASFDEIQE
                     NDFNLNIPRYVDTFEEEEPIDLVAVNTNLLKINEELVQQDQTLLSLINDFSESEENQAL
                     IESMRLLLRGGHDE"
                     /name="type I restriction-modification system subunit M"
     Typing          complement(952..2027)
                     /name="rep_cluster_570"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.536"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttgagcca ccccgttttc tcctctaagc cattgagaag ctaaaaaagg tgttgcggtc
       61 aagcgtgaaa actcgacttt gacttgctta aattctttgt ccgttaatag ttgctcttcc
      121 aacactgcta aattaattcg gttcaaatga cctctaaagt tttgccaaag tgcttcttcc
      181 gacttcaagt ccggacgata agtccactga ttttctttct cacttaagat ttgtatgaac
      241 tggttttcaa tcatttgttc cgaatgactc atttgatccc ccctcatttc catagttgcc
      301 accatttttt agattctgtt tttaccggct ctttttcttt tgccttgttt cgttcttctt
      361 ctgatgactt caattgagct ttcagtcgtt caatttcttc ctgcgcctca ccccttatcg
      421 acgagccatc tttcatatcc tcctgaggca ttttaagagc tttcaattca ttgatttctg
      481 atttatactc ttctagtaat tttttatcct gtaaggctag acgttgttgc tggtctaata
      541 gcttatgaag ctcttttttt tctgattgga gttcggaaac tagattcctt aaaaataaaa
      601 tctcttcatt cccctctgga ttgccatcaa ctgaattatt cacgttgtcg tcaacaaaaa
      661 ctttttgttg acgtttttta gttgacgttt tgttaaaaaa agcattctta ataacttttt
      721 gaccatcaac gtcaacgtaa acggtatttc ctttttttga cgtaaattga cgtaaatcta
      781 ttgacgattc tttttttatt ttttgccata cagcttgctt tgaaacgcct atttcatccg
      841 caatttcttt tatggttttt aaattttcac tcatggattt ctcctttacc tcgaacactt
      901 gcacgttctg gttgttcaag gtcttgtaat tttacagttg gcaggtattg ttcaattgct
      961 tttttgagat acttagctat attgcgtttt gaataggctt cttgtttagc tctcacataa
     1021 gagaggtggt ctttgacccc attcaaccct cgtaaggctt tcagctcgtc atagagaggg
     1081 tacacgtgcg cctgcaatcc tgccataagt gacgtatcgg tcatttcata aggacttaat
     1141 agcatattat ccaacaataa ccgtgtgtac ttgctttcca ttgcctgctt aactagaacc
     1201 gcttcgcttt gttctttttg ctttttatct acttgaaaag ccgtatcttc caacttgtag
     1261 ctgttatcgt ccgctcggcg tttcttctca atatgaaaaa caatagaatc aatcgaacgt
     1321 ccttttttga ctttctcata agacacgtta aaggatgtat gggcgttgat ttcttcaatg
     1381 gcatttttta atacacggtt ttcaaaactt tgaaaacgtg tatattcatt aatcgtatca
     1441 gtgatttctc gcaactcttt cactgtgatt gaggggttgc ggtaggcttc cacttgctcg
     1501 gctctccgtc cacctttaac actataatgc tcgtactggc tataattcat agctaaccaa
     1561 cgatataaga taagactgta cttgctatta agttctttca attcagagat tttatactgt
     1621 gtaaattctg cctttaaatc aatcagataa ggcataataa attgattaaa ttgtatcatt
     1681 acatcatcat tgtagctgtt ccatttaaca gttggtattg ggacgatact tgtcatttca
     1741 taccctttat ttttttcttc tttaatttga aaaaaagctt gtttctgcat atactcaatc
     1801 gcttctttaa aacgagtatg tttacttgct gaagatacat caaaaaaaga aaataattct
     1861 ttttttgaaa gatagattat attatctttg ggcggatttt ctgtatcaat gcaagacacg
     1921 gctaattcaa acatttttaa agcggtcttt tgcattttag cgattgaagt gatcaagcta
     1981 ttatgctcga ccactttacg tttttcgatt tcattcaagg tatacacctg cttttgattt
     2041 tctgatataa tagacataga aatactcctt tgtgagtgtt ttgtgattaa gcatagagaa
     2101 gtgatttaga cgtcaatttt ctctatgctt tcattataca aatatatagc aacttaatca
     2161 agtttttgtt gctatataca tcgttttttg ttgctatata catcgttttt tgttgctata
     2221 tacatcgttt tttgttgcta taaaaatctg tccttccttg ctaccactga ctttttttcc
     2281 acgcaaaaga aacaaaagaa taaaatatat aagataaatg taatataggc ttcgcctttt
     2341 ttttattttt ttcaaaaatg taaaaataaa ggtcaaagtc atcaaaaaag gagtgagcga
     2401 aaaccacgct cacattttac ttgcagcgtt cacttcgttc actcgctaaa agaaaagatc
     2461 aaaaccactt ttaggaattt attggattcc ctctaattgc tcatataagc tgttttaact
     2521 gtttgactag ctaaatactc atatcacgca atcaagcgtc ttacagacgg tcagggcaat
     2581 ccctaacaac ccaaaagcag aattagaaca aaaaccccct taaaactaaa aatagagctt
     2641 tgtggagtat gttacttttt tatttacagg attccttttg aataaactag cgcacccaaa
     2701 aagaagtaag caccctaaaa ttaggttaaa atcaatttaa acactaatgt caagctgata
     2761 caggcttttt aaaagtaaag agcataaatt ttacacctaa agctcataaa acgccttaga
     2821 acgcatttta tgagctttca aaatgatttg ctttttattc gcacgtttat ttttgattaa
     2881 actagcgaag tctgtttaat taaaagtaaa tgtttagttg agcaaagcga aacgaaacca
     2941 gaagggagca aacaatatga aattagatta cagaaaaaca tttgaaattg agataatcaa
     3001 tgaatttcaa tcagcgattc actcaaaaat gttgaacttt gttttaaata atgaattcga
     3061 taaaagtgat tctaaaaacc tacaaacaaa cttactaaat caattatcaa acatgaatca
     3121 aattaattta tttaaattat ctcttgaaga actagaagcc tatcatgagt atttacgagc
     3181 gataaaaaaa tatgctgata gcatcacatg aaaaataatt tcacaatttt aaaatagctt
     3241 aatacttgaa aattgccctt ttttgactaa aataataatt gaaaagaaaa tatagaaagg
     3301 attgattatt atgtcaaacg agattcaaaa acgtaactca gacacatata acatgacacc
     3361 ttttaatttt ttcgaagact ttagtcgtaa tttattcaat gattttaagc caaatttaat
     3421 caaaacagat attcatgaaa ctgataatga atattttgta gaagctgaac ttcctggtat
     3481 tcctaaagaa gacattcaag ttacttacga aaacggagta ttaacaatta gtggccaaca
     3541 ccaaattgat acagcaaccg aagataaaaa aggaaggttg attcgtagcg aacgtagttt
     3601 aacaagtgtc cgacgtcaat atttattaga aaatgttaaa gaagacgaaa taaaggcttc
     3661 ttattcagag ggaattctta aactaacctt gccaaaagat agtaacaaag aaataaaaaa
     3721 atctattcca attgagtagt aattaaaaaa ttacgaaaaa aaggcgccta taaaatttta
     3781 tagacgcctt tttttcgtaa aaataaatta ttttttcttt ctccattcac tatctaaaat
     3841 cgaataaata atttgatctt aataaccatt atcaacatgg gtctccattc ccataattat
     3901 attaatatca tttggtgttg cagcacagaa tttcagtctt tcagtttcaa aaactatagc
     3961 ttgaaacata gtaccactcc ttttctatca tttttatata ccaaagggcg cacttatacc
     4021 caccaaattt ttcttacgaa aaattgatag gtttgtgcta aaaaagagta cagatcactt
     4081 acgtgaagct gtactctttt tccgtcaagc gtgtttgtta aaattcgcta cgctcttgtc
     4141 taaaattatt tatcaaaaaa atctttataa ctcatcttta ttccgaatag ctcgaggact
     4201 ggatttattt tcgttaatat atctttcaat atcggtttta taattataag ttctatcgac
     4261 taaatcataa ttcaaatgag agcgtgacac gtcaatctct ttattcgaat gattgtcagt
     4321 ttttgtcagt ttttcattgg ttatgatttc ctagtccgat taaattagga gctttcattt
     4381 tttgcattct cgctactacc atggacataa aaaaacctcc tattgttggt cttgattcgg
     4441 tacgacttcc aagtaaagta taacccacta tactttacgc cgtaaagtgt ggtctctgcg
     4501 aggctaaaaa ccgtctgtcc actctgcccc ttttttcgcc tttaggctga aaaggttcga
     4561 cgtgaacagg cacgaaaaac gtcggaatat ccgactattt tttccgtgca aaacacaaaa
     4621 atcccctttg cctttttggc aggggatttt tcttaaacta attaatcgac acttacaata
     4681 catgaaattg cttataatag tgtattggaa ttaaggtaga ttgacaaacg agtgatcaaa
     4741 tagatatact tcattcaaac aatcgtttga atgtgaggtg agatgaatgg aaaaagtttg
     4801 ccaagtggct atcgtacata aagaaaaagt aaatgtaata aaaaataaat tggatcaaaa
     4861 agatttttca aacttacttg tgttagggaa atgtttcagc gattcttcta gaattaaaat
     4921 tttttatgct ttagaaacct ataaggaaat gtgtgtctgt gatttagcag aaatacttaa
     4981 tgctagtgtc gctacaacgt cacaccattt acgtttttta aaaaaacatg gaatggcaaa
     5041 atcacgtcaa gatggaaaag tagtttatta ctctttagcg aatgaagaca tcctttccgc
     5101 tattcatatt tttttaaagt tatcagaaaa tctatctatg aaatcttagt ttggaggaag
     5161 ttattttgtt acaaagtatt ttttcagcta tcgctgttta tatttctacc agcattgatt
     5221 atttgtttat cttgctgatt attttttctc aaagtcatac aaaaaaaggg ctccgtcaga
     5281 ttttttgggg tcaatatcta ggaacaggta tcttagtagc cgtaagttta tttgcagcat
     5341 atgtgctgaa tttcattccg caagattgga tcattgggct tctcggtcta attccgattt
     5401 tcttaggaat cagagtggca ttggttggcg aagaggagga agaagaagag gaagtcgttg
     5461 aaaagcttga atcaagagga acaaaccgct tcttttggac cgttgcctta ataacaattg
     5521 cttctggtgg cgataactta gggatttata ttccttactt tgcctcgtta tctttttcag
     5581 agattgtcac cgctctaatc gtatttgcta tttctgttgc ggttctttgc tatatcagtt
     5641 acaaattagc caagatttct tttgtttccg aaaccttaga aaaatatgaa cgaatccttg
     5701 ttcctatagt gtttattggc ttaggaattt atatattgat cgaaaatggt acgattcaaa
     5761 ctgtattgag tttatttaat taataaatag tttgattgtt agtacaaaaa aaagacagcg
     5821 attaaaactc gttgtctttt ttttgtacta acagattggc gcattatcta agtaatataa
     5881 ttcgatacat gaaattgtat aaaatcatgt atcaagaatc ctatacctat agggttcttt
     5941 ttttataccg gtgtccaata catgatttcg tggtataatg aacgaaatca tgtatctgtt
     6001 ttgtgagagg agagaccatt tcaatgagtt ataatgtaca accattacga acgcagcaag
     6061 aaatcaacga ctttttattc tgtttgagac gcaacaaaaa tgccgaacgc gacgtttttt
     6121 tgtttttgat cggcattaac agcggtttgc gcatgtctga tatcgtgaaa ttaaagaaaa
     6181 aagacgtgat ttcctcgaaa aacccacgta ttgtggagca aaaaaccggg aaaacacgca
     6241 ttttatattt aagtagcctg caggagttga tccaggacta cacgaaagac ttggagccag
     6301 aggattattt gtttcccagt accaaagggg gccatgtaga agtcaataca gtctatcaga
     6361 tgtttcaaaa ggtcgctaag ctgttaggaa gagacgatat tgggacgcac acgctgcgga
     6421 aaacgtttgg gtatcattat tacaaaaaga ccaaagatgt ggccacgtta atggaaatat
     6481 ttggtcatag cagtgagaaa attactaaac gctatatcgg aattaatgaa gacgagatta
     6541 gcgaaacatt gttgaacttc cgtttaggtt tctaacttta agacaatatt ttcaagaatt
     6601 cattgttttt tgatatctag ataaattcac gaaaaaagta tctaagatag catagatatc
     6661 ttagatactt tgattatttc taaaatttcc accacttcta aggttaattt ttgattggtt
     6721 cgcttgattt cagcgtttaa ctgcttaaag acttggtcca tggtctcggt tatggtctga
     6781 ttgatctcgc ttaattggtg gttgttctgg ttcatttcgt gttgaatttt cgcgatttcg
     6841 gttttgtttt ggtctaaaag atcgtttagg atcgttttca ggtgtttcaa ggatttgttg
     6901 tcggctagcc gacttctgat acattttttt aagcacaaaa accacccaat tttggagtgg
     6961 tgtgtaagtg cgcattgttc gaaaaatcga actatgattt atttttgctg ttgtatttat
     7021 ttttcatctt ttgggttttg gttttgtttt ttattgctat tggagtttat ttgcttttta
     7081 agggctctat ttttcgttct acggcatttt tataatttgc caatataatt ttatcgattt
     7141 tttattaaaa cgtctcaaaa tcgtttctga gacgttttag cgtttatttc gtttagttgt
     7201 cggcataatc gtcaaaacag gcgttatcgt agcggaaaag cccttgagcg tagcgtggct
     7261 ttgcagtgaa gatgttgtct gttagcttat gaaagccgat aattgaatga aataataagc
     7321 gtagcgcccc ttatttcggt cggaggaggc ccaagggagt ttgagggaat gaaattccct
     7381 catggtttta aaattgcttg caattttgcc gagcggtagc gctggaaaat ttttgaaaaa
     7441 aatttggaat ttggaaaaat gggggggtat acgaccagca cgaaaactat caagtagtgt
     7501 atcctgcacc atttgtaaga tatttgaatc tttgataggt aaaacaatct gttgaaccat
     7561 atatcatata ttcctttcaa aaaaataagt aaataattaa attatttatt tattttcttg
     7621 tcggtgtctc atttctgcac atccgcttgt agcaagggtt tcatctgtta tttttttgaa
     7681 aacaactttt taattgctcc cagtttacga taagaaaaca aacagggata aaaatgaaca
     7741 tgattgctag taaaagcatg cctaccatcc caaataatcc ctccatcttt ggttctatta
     7801 tattaaaaaa acataaaaac aacccctatt acctgtccaa tagataacgg gggttgtttt
     7861 aatagtcttt ttgaggggtt attatctaat tatagaccct aaacaaacat cttttgtaaa
     7921 aagccttttt tctgttcttt caataaatct aacttacgtt gatgaagagc gatagtatca
     7981 tctagttttt tgaaaaatga accgatctta gcttgctcat cactccctgg cacgctaatc
     8041 gtaaatacat tcagaaagtc tttttggacg tgcggtatat taggcgtccg ataattgtta
     8101 taaatattat cctggtgacg tttcaaatat tggtaaacaa attttgagtt agcagaggtc
     8161 ctgtaagcct tgagtgtaga gcctaaggcc ccttcaaatc catgataaac agttcctgct
     8221 tttgatccat cccaaagaat taaaatatca tctaatgtta catctttaag accgttagtt
     8281 aatattgctt ggccaccatt caaccttgat gtatcaagat attcaacatc tccctgggct
     8341 aatttttcaa attgagcctt accttttatc tgcttttcaa cgacatcctt taacttacgc
     8401 tcttcccaat cgtcagcaaa ccctgcaaat cgcaattcag gaactttggc accattttta
     8461 gggaacattt tttgtaagta gccttttttc tgctctttca acaaatctaa cttacgctga
     8521 tgaagagcga tagtgtcatc taacttttca aaaaatttag aaatagcaat ttgttcttcc
     8581 agcttcggaa tgtataaatc tatatcttcg agataagaat aatataggtt tgatacattt
     8641 ccaccttcta ctctcttcgc aaactcatat ttcatttggc ttctaaaata ataagatata
     8701 aatagtggat tttcatttgt attataaact gaaatcatct caccaattgc aacatttgga
     8761 atactaagcc atgaacacaa tgagaaatca agtggatttt cgcctacccg aggcactaaa
     8821 acttcattac ctgtactaaa ttttagattt ttagggttta tattagtatg agaataaata
     8881 ttagatattt ttgctccgta ttttgtatat agttcaccat atctaacaca aggtgtttcc
     8941 gcatcctcag tcactgacca tttcggtgcg cttttcccat aataaaaata gcctacttcc
     9001 cctaacttac gctcttccca atcatccgtg aatcctttaa accttaattg tggacttttt
     9061 ttactcatcg tgaccgcctc tcaaaagaag acgcatcgat tcaatcaagg cctgattctc
     9121 ttcactttct gaaaaatcgt taatcaaaga taagagtgtt tgatcttgtt gaactaattc
     9181 ttcattgatc ttaaggagat tggtattcac tgcaaccaaa tcaatcggtt cttcttcctc
     9241 aaaggtatct acataacgag ggatatttaa gttaaagtca ttttcttgga tctcatcaaa
     9301 acttgcaaca tgagcatatc tttcaatatc ttctcgtttt ttgtagatag aaacaatttt
     9361 atcaatatgt tcatccaaca ggacattttg atttttttgt ttttcaaaat cttgagaagc
     9421 atcgataaac aagacatcac gccgagaacg gtttttcttc aaaataataa ccgttgtcgg
     9481 aatactggtc ccaaaaaaga tattggccgg caagccaatg accgcatcaa tggctcccat
     9541 ttctaaaagg gcttgacgaa tggttccttc tgcggctcct ctaaagagaa cgccatgcgg
     9601 aaggacaatt cccattgttc ccgattcttt caaatggtaa aaaccgtgaa ggagaaaggc
     9661 aaaatccgct ttagatttag gcgctaattt tccaaaacgc tcaaagcgag ggtcagagag
     9721 aaatttatct gccgctgacc attttgctga gtaaggaggg ttcatcacca cagaatcaaa
     9781 ctgataaggc tcttctgatg gccaatcggc atctaaagta tccccattat taagattcat
     9841 gcgttcttta tctaccccat gaagaattaa gttcatacgt gccaagttaa aggtcgtcgt
     9901 atttagctct tggccatgat aatggacttg attagggttt ttgagataac gacgaatatt
     9961 aagcatcaaa gaacccgaac ccatcgcagg atcgtaaata tgaaaaggcg ctcgagattc
    10021 ttgtccgatc gacgtaattt ctgacatgat gcgtgaaaca gcttgagggg tataaaactc
    10081 tcctgctttt ttacctgcac ctgccgcaaa catcccgatt aaatactcat aagcgtcccc
    10141 aatcacatcg ccattgtgtt caaataagtc aatctcatct aaggcacgta aaacctcagt
    10201 aatcgtcaca ttacgttgtt gtgcatttga gcctaatttt gtggagttta aatcaatatc
    10261 cgcaaacaat cccctaaatt cttctccttg acgttccaac tcattaaaac ctgcttgtaa
    10321 gtcggtcaaa ttaaattcat agttatcggc tttgatacgg tagtgataaa acaattgatc
    10381 gggttggata aagtaacctt gtttgggttg gatattttcg attaaatcgt ctttgtcttc
    10441 ttcgtaccac tccatgaagc ctgcatactg agtggaacgt tctgggaagg tatctgtttt
    10501 tccattttct tgttcataaa cttctctaag ctgcgcatca gacaagtact tatagaaaat
    10561 taatcccagt aagtagtttt tgtactcact tgcatccatc ttcccacgta agatatccgc
    10621 agaagcccat agttgttgat ttaaacctgt tgccattata attcttcctt ccatttttga
    10681 agatgttcaa aattttgttg tagtcgttct tctaaaactt ttgttcttct tctgtatttt
    10741 tcacccgttt tggcttcgaa ctcttcttta ctaagagtca tcgaatccag taaatgggtt
    10801 aaataaggga tcgttttctt atcgccttga tactcattca ttgcagactg taaaaagtcg
    10861 aaggataatc caaattcttc agcttctttt tgaagttgtc tttgaatttc attttgaatc
    10921 gatgtctctt ttaattgagc tacatcaatc tcttcagcag tattgacgag ttgatctttc
    10981 aaggtgtgat acatcttttg gatttgagga tccttttgtt ggatttcttt ttcaaatttt
    11041 tcaacggccc ctgcttcgtt taattggatc gctttcaaaa gttgattaat ataaaaacta
    11101 tccacgacgt ctttatgtgt tgcattgagc tgcgaagaaa aagcaatctc ttgtaaaagc
    11161 ttctcgaagt cttcctcagg atgcccctca tcctcgatca tttctttaat ctttgtttta
    11221 atattttctg tttttccttg atattgtggc aactgctcca ctacttctga aaatgcttca
    11281 aagtcctctt cgtattgatc atagcttcgg agcgctttat aactcttttc tagcttttga
    11341 taagcactta cttgcgcaat catcgtttta agatcattgg gattatccga taggacagct
    11401 tcgctttgtt tataaagtgt tgaacattcg ataaattctt ttttgacttc ttcatattct
    11461 cttggaatca gttgatcaaa gttttgcttt tcatttgaga atagacgaaa cgtgttttgc
    11521 acattttctt tcatggtgaa gggctttcta aaagagacaa tcaacccaga atctttgccc
    11581 gtataaatac gattcgttct tgagaacgct tgaaggagct tttgatagtt catttctcga
    11641 tcaatatata aggtttgaat cgttggagaa tcaaagcctg tcaataaacg atcgacaaca
    11701 ataacaaaat ctagccactg accatccgat tgatattgct tttccttacg ggccaaccgt
    11761 ttattaatat tttgattgta gagtttctca tcttgataag gagaagcatc aaactgtttc
    11821 gcatactctt tcatgatctc tactaattca tcgtcttgtt catttttttc taattgatcc
    11881 ggattggttg agaaggtaat agctactctt gggaaatctt tatcaatcag ttgatgacgt
    11941 tcatcaaatt gtcgcccatt caagagtgtc ccattatctt tcatttcctt taaaatccgg
    12001 taaatatgtt tggcttgggc aatcgagtga gtggtgagaa tggctgacat cgtaggaaaa
    12061 ccattcttta ccttgaactt tttgaccaca cttctccgat taaagatttt ttgtaacatg
    12121 gtacgaatat gttcatctgt ttcataaagt tccgtaggca ataatttttc ttgttggagg
    12181 gcatcgtctg gcaactttcc tttattgagt tgggtgacaa tcacctcttg atcctcttct
    12241 gaaatcagtg aatgatactc gacttgaaag cctaaaacag ccccatcatc catagcattt
    12301 ttggttgtat aggagtgaag gagtggcccg tattgctggc tcgtcgttct ggcaaaggtt
    12361 ccattttctt gctttttatt ttcttcaaaa ataggcgtcc ccgttaatcc aaaccaggtt
    12421 gaattgggta atattttctt aattcgcttc atctcctcat cactaaccgc acgatgagcc
    12481 tcatcaacaa taaaaacaag atgttcttgc cgtagcttct caaattgatt cgagcctttt
    12541 tcttcactct cttgttgggc gcttcgcata gccgcagaga gtttttgaat cgtggtcact
    12601 aaaatcgtat tattattttt tgatgatagg aggtttcgag ccaactgttt ttgatttttg
    12661 atcccaacaa tcaaagtatt ggctaccgaa tttccggtcg tttgtccggt atggtattcc
    12721 gaggcaaact tcgtaaactc atcctgcgtt tgagcatcta agtctgttct atcaacaacc
    12781 atgaccgtac gatcgacacc gatcgcattt tgtgctaata atttcgttgc gacaaaactg
    12841 gtaatggtct ttcctgaacc tgtcgcatgc caaatgaatc ctccttcatg ctgtgtcgct
    12901 tttttacgaa tcttacgaat agcatgaatt tggtaaggtc ttaaagccat gaggaatttt
    12961 tgattttttt gatcatcgac gagaatggta tattggctaa tcaattcatg ggcatcgggt
    13021 atccgcaaaa ctgtacgagt aaaatcaaac aaatcggaaa ccgtttgatt gtcttcagtc
    13081 cgccaattaa ataaaaactt cttcatccta gcataggctt cagcggtatc ttcactaggt
    13141 cttgcaaagt atcgggtatc gactttattg gaaatcacca tgatttgagt ggttgcgtaa
    13201 atccctttaa aaaatccatc ttctgcataa cgctgaattt gataataagc ttgcatgaaa
    13261 ccgtctttag cggactcttt tttgagctca atatgaatga ttgggagccc attaatcagc
    13321 aagctcacat ctccacgatc tactctagag gaatctggga ccacttggtg aactacctca
    13381 taagaagaag ttcctcctga gatatcctta tttctaaagg cttctaaagt cacttttttc
    13441 ccatcttctc gctctaataa a
//
