LOCUS       NZ_CP110061            19782 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Enterococcus faecalis strain BE16 plasmid pBE16_2, complete
            sequence.
ACCESSION   NZ_CP110061
VERSION     NZ_CP110061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31342461
            Assembly: GCF_025958805.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19782)
  AUTHORS   Gaeta,C., Pinzauti,D., Marruganti,C., Bollini,R., Fabbro,A.,
            Santoro,F., Grandini,S. and Pozzi,G.
  TITLE     The presence of Enterococcus faecalis in saliva as a risk factor for
            endodontic infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19782)
  AUTHORS   Pinzauti,D. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2022) Dipartimento di Biotecnologie Mediche,
            University of Siena, viale Bracci 16, Siena 53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65x
            Sequencing Technology :: Oxford Nanopore GridION, Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:44:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,933
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,818
            CDSs (with protein)               :: 2,818
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 19 of 37
            Pseudo Genes (incomplete)         :: 17 of 37
            Pseudo Genes (internal stop)      :: 5 of 37
            Pseudo Genes (multiple problems)  :: 3 of 37
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19782
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BE16"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pBE16_2"
                     /geo_loc_name="Italy: Tuscany, Siena"
                     /collection_date="2019-04-18"
     gene            complement(join(19773..19782,1..824))
                     /locus_tag="OLL92_RS14510"
                     /old_locus_tag="OLL92_14510"
     CDS             complement(join(19773..19782,1..824))
                     /locus_tag="OLL92_RS14510"
                     /old_locus_tag="OLL92_14510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_264547825.1"
                     /translation="MIEKMVAFMRDENNEDFFCEGFYHTILFVIVYVLYLTYKESAIGN
                     SNYIFLFFFVLTSYALIKYFLSCKQKFAPITKKQYPLSIKILAGYLINTGIWLAVTSMM
                     MFFYGKIEILNQQLLDKQITDHPKMLVPQLFDTVILAPLMEEILYRGLLLNAILFIGLK
                     LGVSKKYLFVVFLVLSALIFGSIHASSNILTFIGFSLIGLNLGALYLISGNLIVPITVH
                     FLNNLSASLNVSNDVKRISIFICLLLIICLIEYVCKEDRNIIKKWAFSLIKKDKP"
                     /name="type II CAAX endopeptidase family protein"
     gene            complement(891..1193)
                     /locus_tag="OLL92_RS14515"
                     /old_locus_tag="OLL92_14515"
     CDS             complement(891..1193)
                     /locus_tag="OLL92_RS14515"
                     /old_locus_tag="OLL92_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058221280.1"
                     /translation="MAKPEFKKAVEVSKKIDGLTQPKSFLQESSKNKKWEEYEKEIEKQ
                     QAEYEQVIYSVAMEPALKEAIKIAGKKKGKLQGGAKAVIREALVEHFKKNKDYFS"
                     /name="hypothetical protein"
     gene            complement(1198..1965)
                     /locus_tag="OLL92_RS14520"
                     /old_locus_tag="OLL92_14520"
     CDS             complement(1198..1965)
                     /locus_tag="OLL92_RS14520"
                     /old_locus_tag="OLL92_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002415477.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_010783638.1"
                     /translation="MAKIIAFYNNKGGVAKTTTATNVAGVLSLQKKKVLLIDGDPQGHT
                     SLTFGVDSDDLQTTLGAYLSSNWTASEASQYFIKINDFLDVIPSNQSLSDFIILTSSED
                     PKIRNKHLKNFINPIRKEYDYIIFDMAPAVDIILENIVEIVDDLVVVATPETYAVKNAE
                     TTLKITDDKQVTVRHIVPTKTQMNTKTHKFMLENLQEVADSHHINMTKTYIPNLIAFSE
                     AVSIYELPLALVNDKRYLKAQEYYQNLVEELGY"
                     /name="ParA family protein"
     gene            complement(2075..3232)
                     /locus_tag="OLL92_RS14525"
                     /old_locus_tag="OLL92_14525"
     CDS             complement(2075..3232)
                     /locus_tag="OLL92_RS14525"
                     /old_locus_tag="OLL92_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877419.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_214592765.1"
                     /translation="MPIISEKQENQKQVLTLNDLEKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCIDTEKQLEDNTVYLSKRDLFAFFKVSDNDKHSRFKEAVEKMQKQAFFKIKEK
                     KEHGFEFENIVPIPYVKWTDYNDEVLIRFSPEIIPYLVNLKKNFTQHALSDIAELNSKY
                     SIILYRWLSMNYNQYEHYSVKGGRREEQVESYRNPSITVKDLRIMTDTIKEYSRFQSFE
                     NRVLKNAVVEITQHTSFNVSYEKVKKGRSVDSIVFHITKKPVARNDFYKLEEQDPVYLQ
                     DKENKEQAEELLAGKALKSKYTKLLLDNMLLSPYEMTDTSLMAGLQAHVYPLYDELKEL
                     RGLNGVKDHLSYVRAKQEAYSKRNIAKYLKKAIEQYLPTVKLQNL"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(3922..5433)
                     /locus_tag="OLL92_RS14530"
                     /old_locus_tag="OLL92_14530"
     CDS             complement(3922..5433)
                     /locus_tag="OLL92_RS14530"
                     /old_locus_tag="OLL92_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_264547824.1"
                     /translation="MTVINMPNKSKGTLHSKQGLSSALKYITDPQKTNNGELVSGQNIN
                     IPSNAYDEMLLTREMAILAGNQPKENERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAY
                     LGESAEFVIATHTDKEHLHNHIILNATNPKTLKKFQQSKSQLEELKEISDVISKEYGCK
                     IIDRSLKNSHKKYQVYLAKNSYRKEIKSKIKFLLSHASNWNDFKNKATTLGLIVDDKGK
                     YTTYQLSGTSQERKVRDRSLKNQAFMKESISERLKENEVVYTESEVKELWAENQTIQDK
                     EQETEIEMLIEDWQVEKETADYLYVNINIGLEREQRKSAIKIPARCVDKLENGNYQVFL
                     KKQDRFYFVDSKNPENNKIMLGKTVAKNLQNQSGSVPLYSNNAKIKLKQLFNEFDFLIS
                     KGLDFDTSFETIGKELEQTYYQTESILENLDKKILEYVEENKAYARPNTRILKTIENLQ
                     KERTDLHKTLKQVEKEMLYYDKSVDRYKDYQQEHTKNKSQHPPRL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5438..5806)
                     /locus_tag="OLL92_RS14535"
                     /old_locus_tag="OLL92_14535"
     CDS             complement(5438..5806)
                     /locus_tag="OLL92_RS14535"
                     /old_locus_tag="OLL92_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_264547823.1"
                     /translation="MDKKANRQRNIQKKFFVSELENKIIEKRMQEANLTNFGEFARKSC
                     LDKKIYTVDFSSLKEIMSTIAQANLEVNRIGNNLNQIAKYLNESEQNQTRELLNEYQKE
                     IQSLDQKLRNMLKKITEG"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5999..6280)
                     /locus_tag="OLL92_RS14540"
                     /old_locus_tag="OLL92_14540"
     CDS             complement(5999..6280)
                     /locus_tag="OLL92_RS14540"
                     /old_locus_tag="OLL92_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251846361.1"
                     /translation="MNEQEIMAEVEDYGRQIFEAISYANEFPVVKEKLLIMFDKLIDEL
                     SELIDEDELNDYKKAKEVVEKIPENEVEELCFTVESLYGDVENYPSYF"
                     /name="hypothetical protein"
     gene            complement(6286..7320)
                     /locus_tag="OLL92_RS14545"
                     /old_locus_tag="OLL92_14545"
     CDS             complement(6286..7320)
                     /locus_tag="OLL92_RS14545"
                     /old_locus_tag="OLL92_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396409.1"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="WP_264547822.1"
                     /translation="MGDNKEQTDIQSLLAQKDTSKLSSFLKEQIVEYKNSEMFKNYLDF
                     VAKCPRYSNRNIRFLIKQKPDIGRVGTFTKWKEQGLHIKKGSKGYKIFMPIIKDKYENG
                     KPVLDEKGKKVREVKGFKLGTVFEDKQLVEYENLPKQVEYLNQDPNNTKDLFMALSEVS
                     EVKIGLKPLEVDGFYSPQDKCIFINANLHGADLIHTLIHEVTHSKLHTKSEAIFGDKQY
                     TLQELEAESTAYIVANNYDIDTSKYTMGYLNSWGLDKISNEELQGVMENIQKTARGIIE
                     KIDLELEKRKNIEPTKSKLQTEIQEAKKEQKELLKKHEEKQKKKPKKESKLDSFKNKFK
                     GKGE"
                     /name="ArdC-like ssDNA-binding domain-containing protein"
     gene            complement(7356..8159)
                     /locus_tag="OLL92_RS14550"
                     /old_locus_tag="OLL92_14550"
     CDS             complement(7356..8159)
                     /locus_tag="OLL92_RS14550"
                     /old_locus_tag="OLL92_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_264547821.1"
                     /translation="MKKKAIFTLFSTALLLGSTGISSVSVFADELPKTAEPTQPAPADP
                     TEPTPTDPVNPTPEPQPEPQPAPQPAPQPEPQPEPQPAPQPEPQPEPTEPTEPTEPAEP
                     TEPTEPTEPTEPAEPTEPKEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTE
                     PSKPVDTNPIENPVNTDTGEVIVAVEDSKPIIQLADGTTKKVEAKEIGANVQKDGTVTV
                     KGSDGKMKVLPKTGETENIALSIIGSLSTLASGFFLFNRNKKGQA"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            complement(8183..8989)
                     /locus_tag="OLL92_RS14555"
                     /old_locus_tag="OLL92_14555"
     CDS             complement(8183..8989)
                     /locus_tag="OLL92_RS14555"
                     /old_locus_tag="OLL92_14555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251846354.1"
                     /translation="MMNKQQSKYVFSETDIKKFVDESVVSIPFEDFVTLIIDTNAQLLK
                     TSSLRSKSVLISKYTKDDILIYSYKLELPQSKDVSFDNILEPFSKNKPEKKEIRTKKIS
                     LNVNNVLIVLSLILAITSLAMSVNSKTKEVTNKKLENKVTTLEKSLNNQKVVLDNQNTL
                     LENKGKIDVYVRYFLPYLFSENKISLDDFVSDEISKDIKHQQGSLISVMQEEIKLDKKG
                     FKAIYVISVKEENINKTKRVELDIEKNKSKKFEFEIKEIPKIQEIK"
                     /name="hypothetical protein"
     gene            complement(8967..9206)
                     /locus_tag="OLL92_RS14560"
                     /old_locus_tag="OLL92_14560"
     CDS             complement(8967..9206)
                     /locus_tag="OLL92_RS14560"
                     /old_locus_tag="OLL92_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251846355.1"
                     /translation="MNNQTLQERQQGLLKQQKDLLKKRDKINQELKELKKKIEDSEKDY
                     LIQKLKEADISVTEAIELLGIQNRGNTHDEQTAE"
                     /name="hypothetical protein"
     gene            complement(9203..9556)
                     /locus_tag="OLL92_RS14565"
                     /old_locus_tag="OLL92_14565"
     CDS             complement(9203..9556)
                     /locus_tag="OLL92_RS14565"
                     /old_locus_tag="OLL92_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_251846356.1"
                     /translation="MNKKNIGSTLFFLILLLFAGFIVYDRHQTESSTAVVSTIKEDKIN
                     FFYRDDCKDCKKVFNQVYMHNLINHDVQFINLNQEKNRKYIDEYNIHTVPTFIHENEAY
                     AGTQKEKIKEILK"
                     /name="thioredoxin domain-containing protein"
     gene            complement(9549..10130)
                     /locus_tag="OLL92_RS14570"
                     /old_locus_tag="OLL92_14570"
     CDS             complement(9549..10130)
                     /locus_tag="OLL92_RS14570"
                     /old_locus_tag="OLL92_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062895.1"
                     /translation="MEKKIFIMKIISGACIFCFVGMLVFYITLVNSTNKIIGEKDQKIS
                     TLTKQNKELDNKYNELYEQKNGNANEQLVLATKGVFSSIFNYDTKKDTITDRREKARKF
                     TTEEALETIFPKEATSYNASVQTVSKLKGEPQIFYAPTNDKKQKVLIVVDNAITIAGSD
                     PQEATFMYRAEFDPVKNQFVSLKNAGTLNE"
                     /name="hypothetical protein"
     gene            complement(10134..11336)
                     /locus_tag="OLL92_RS14575"
                     /old_locus_tag="OLL92_14575"
     CDS             complement(10134..11336)
                     /locus_tag="OLL92_RS14575"
                     /old_locus_tag="OLL92_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="WP_251846364.1"
                     /translation="MIISLFIGVKNDQCSKPSYSDNTITENTSKPPKSVDDFIRSHKEA
                     YILSWKAGGFLPSASIAQTMVENGFNFTNPQGTSLWKAHNMGGVKTSKKEDFPVTLASF
                     GADSVDLTGTKAGTNVGDGTGGGYAWFKDYNAGIVGKAEFMAHQSLYRGAINNTDGKAT
                     LMAIANGGWATDPNYLISLLGAYNSIGQKYQWIDREAIEKHGEKPYQSNQLLDKKAIPN
                     MPTKEEASQGLSEEGCVVNQTPSKEATGTNSAPTLKPPKEYEGKLKLPAPDNKNYAGNN
                     YPFGQCTWGVFNRMAQLGTPIEWFSGNGGNGGFWWQSAKEKGYNVVKGNPKVGWAVSFT
                     GGLAGSDPQYGHIAVVEFVNDDGSFLVSETNVVSPSSGTRSWRVINKATAQQAYFIQGR
                     K"
                     /name="CHAP domain-containing protein"
     gene            complement(12018..14399)
                     /locus_tag="OLL92_RS14580"
                     /old_locus_tag="OLL92_14580"
     CDS             complement(12018..14399)
                     /locus_tag="OLL92_RS14580"
                     /old_locus_tag="OLL92_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="WP_264558086.1"
                     /translation="MNVKSLFNQKKSKKQTPLSKTEKLRLNRLKKNAQKAKSSSSQNTL
                     RYTSLFENGLMNIVDDNYSMTYELGSTNYATAPNEEKTNIIQKYNTMINTLSDNEHFQL
                     TLLVSKVHEKEYQEKNEFPLQGDSYDYLREELNELINLNYQKGLNNYQIDRYITISTNA
                     EDKKKALGKLENTRNLMSSTLASIEVPIKPLDGLERLSVMNKMLNYDKPLYGNFNDIRL
                     SMLTTKDLIAPSSLKFSKTGVEIDDRFNQILFLKDFPMELSDNLFKDLAECGQELVITI
                     QAQPYSIIETNKRLRAQATSVESEIIKQQKIAVKEGYSPDFIGRTSKEAQGDLDEVINF
                     VRETGDKQFSSIFLVYLTGKTIEQRDENTRIIKAIGEKYGAIFSSLGYLQEEALNSILP
                     IGMNFTDCEKTFQRDLITPNLSINSPFTTVDINHKKGKFYGINLFSNNIIAINRRDPSM
                     DNSNGIITGLSGSGKSMTAKYEIITTLLKNPQDEVIILSPDNEYDELANLLDGQLIKIA
                     PKTNHHINILDLPDENLLDSDDEPVGLKSSFLVSLFSSLFENITEIQESVIDEVTIATY
                     KEYSNPTLHNWFDVLQTIDNDEARDLSYKLKLYITGSMDIFAHETNVDFNSRLIVYNIS
                     QLKNKFKPFGFMAIEEKIWQRVVENKAKGVTTWVYFDEIQVLLSGASSESSREKFKDVW
                     ARIRKYGGNPTGITQSIETVLATEEGRAMFFNSEFLVLLKQKKFAIEIIKQHYNITEQQ
                     EQYLRTPAKGSGLIIAGNTIVPFENTIPNTTELYKIMQTD"
                     /name="VirB4-like conjugal transfer ATPase, CD1110 family"
     gene            complement(14335..14703)
                     /locus_tag="OLL92_RS14585"
                     /old_locus_tag="OLL92_14585"
     CDS             complement(14335..14703)
                     /locus_tag="OLL92_RS14585"
                     /old_locus_tag="OLL92_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024078.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="WP_014570276.1"
                     /translation="MYIKVYKDGSKIKSKVAGLTFRQFIFLLSIAGVTGLIMINNFFLH
                     ISSLFFEFPLMLILFISFFVSLVKINGLTGDRWFKLKWNYLKKPTKRTYQTERIVQYER
                     KEFIQSKKVKETDTFIED"
                     /name="PrgI family protein"
     gene            complement(14705..15445)
                     /locus_tag="OLL92_RS14590"
                     /old_locus_tag="OLL92_14590"
     CDS             complement(14705..15445)
                     /locus_tag="OLL92_RS14590"
                     /old_locus_tag="OLL92_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010783628.1"
                     /translation="MDLFKVLGGHADKLNENGWSYVTQVMQNSTTKVGIYLLIFFLIFE
                     IAKIFETANKNNEGTVVPITMFQASVNALLAGALVAGSVFILPFINEIVVGGINLIKDT
                     GSYNIFAPASLQGVSWTDGIGKVISWLITYLVGAGASIIIVVVVSLRTFQLYVYTILAP
                     IAFSSFASSEYRSIGVGFAKQYCAKALQGFVILIILGLSNSFSAPDPKGAVAGGFVSLI
                     QPIIVAVLILQSEKYAKSVVGLGG"
                     /name="hypothetical protein"
     gene            complement(15491..15766)
                     /locus_tag="OLL92_RS14595"
                     /old_locus_tag="OLL92_14595"
     CDS             complement(15491..15766)
                     /locus_tag="OLL92_RS14595"
                     /old_locus_tag="OLL92_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010783627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058221275.1"
                     /translation="MKTTLSNLAFTALTHPVLAQANGLDTIKRLLTWFFGAGSIGLIIV
                     GVIQIAESMAESNGGQRTRGIMLILGGLLLGGAAGLVNLLTNPPGV"
                     /name="hypothetical protein"
     gene            complement(15770..17596)
                     /locus_tag="OLL92_RS14600"
                     /old_locus_tag="OLL92_14600"
     CDS             complement(15770..17596)
                     /locus_tag="OLL92_RS14600"
                     /old_locus_tag="OLL92_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014581.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="WP_058221276.1"
                     /translation="MKDLSKVVKAKIIIFSVLGLILGCFLNRFAYFLLNEKAEGIQKIF
                     NSLANTMDSYSNQWLFFSFKGYPLLAFLLGLVLALLFFMLSTSNGKYRQGKEYGSARWA
                     TSKELKKFADSKNEDNNLIFSEDVKMSLTTKNMDFDYQRNKNVLVCAGSGAGKTEMYVK
                     PNISQLHSSYVLTDPKGKVFHETGKMMSENGYQVKIFDVNTFKNCNKFNPFKYIHDEIT
                     LKRVIQCIIDATNGENSRKGEPFWDKSEELLLGSIFSFLYYKYKGDPKKGITGTGDLPK
                     LYEVSDLIRLLNRENEEVPSVLERMFEKFEDRFGSENYAVTQFNSFKNYRGDTRSSVIA
                     IATARFSMFDLQDIRDILDDDELEIDTWVTKKTIVYLPIPDMDTTFNFLTTMIFVLAFR
                     TLEHQIDNEFHGNPPNHIRFLLDEFANLGKIPNIKEALAVFRSREMSINVIVQNINQLT
                     SMYKEDWKSFVGNCDTIMYLSGGTEPETEKFFSERAGKETINMKKNSENRGSNGSYSIS
                     HEVLGRDLITKDEVNRLPRSECLVSISSMPMYRGKKFPFKGHKRSTEWSSSPSDDNWYE
                     FQPEFKVDELEKAFDYWNSKKPVETLTNTMEE"
                     /name="VirD4-like conjugal transfer protein, CD1115 family"
     gene            complement(17596..18081)
                     /locus_tag="OLL92_RS14605"
                     /old_locus_tag="OLL92_14605"
     CDS             complement(17596..18081)
                     /locus_tag="OLL92_RS14605"
                     /old_locus_tag="OLL92_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058221277.1"
                     /translation="MAEEDETVRLILGVGKSSIRHAYSLLKKHYDNKGKVPNFTDSELL
                     NESLSNSNVAIKALHREYKKNGVDFLSRELPDGKTELLFHAKDRNMILATTEKVMRDMQ
                     ANPQKYLKKEKAIQPDKGKKEPSLKDEIKIAKQEQQEMLKNVADKAVNKTLGKGKSI"
                     /name="hypothetical protein"
     gene            complement(18085..18462)
                     /locus_tag="OLL92_RS14610"
                     /old_locus_tag="OLL92_14610"
     CDS             complement(18085..18462)
                     /locus_tag="OLL92_RS14610"
                     /old_locus_tag="OLL92_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058221278.1"
                     /translation="MNKFLSILHLIFIRPIEILVGISGLIIRFVLISPLFLIQNLITGA
                     IIIWIYGQVVALVTAHQFLPASYFDISKYPSYVQEVLQTKMSFAGMWNWYIMPLAIVLL
                     IEVVISFLVWFIPKELRSGGE"
                     /name="hypothetical protein"
     gene            complement(18561..18851)
                     /locus_tag="OLL92_RS14615"
                     /old_locus_tag="OLL92_14615"
     CDS             complement(18561..18851)
                     /locus_tag="OLL92_RS14615"
                     /old_locus_tag="OLL92_14615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058221279.1"
                     /translation="MHDEKWYSLSEASIKQGRSRAYFSSIKANHPEYFEHVELKKVGDN
                     LVINQRGIDEVLKHVKKAGDRLRILPLKAVYKTKHILCFYPTNHYIKIAIN"
                     /name="hypothetical protein"
     gene            complement(19071..19661)
                     /locus_tag="OLL92_RS14620"
                     /old_locus_tag="OLL92_14620"
     CDS             complement(19071..19661)
                     /locus_tag="OLL92_RS14620"
                     /old_locus_tag="OLL92_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395662.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002395662.1"
                     /translation="MSKIGYARVSSKEQNLDRQLEALQSVSKVFSDKASGQSTERPQLQ
                     AMLDYLREGDIVIVTELDRLGRNNKDLTEIMNAIQQKGATLEVLNLPSMNGIEDENLRR
                     LINNLVVELYKYQAESERKRIKERQAQGIELAKKKGRFTGRKSTFQKDDPLLQRAFNLY
                     QSKEYTLKEIEHHTKIPVSTLKRYLTKYGIKRK"
                     /name="recombinase family protein"
     Typing          complement(2091..3232)
                     /name="rep_cluster_554"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.189"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3926..5433)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.036"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(15774..17596)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttttttga ttaaactaaa agcccatttt tttatgatat tcctatcttc tttacaaaca
       61 tactcaatta aacaaattat taataaaaga cagataaata ttgatattct ttttacatca
      121 ttactaacat ttagacttgc agacaaatta tttaaaaagt gaacagttat aggtacaatt
      181 agattgcccg atattagata gagcgcccca agatttaaac ctattaaaga aaaaccaatg
      241 aatgttagaa tattactgct agcatggata gaaccaaaaa ttaaagctga taaaacaaga
      301 aataccacaa acagatattt tttagataca ccaagtttca aaccaataaa taaaattgca
      361 tttagcaaca aacctctgta tagaatttcc tccatgagag gtgctaaaat aactgtatca
      421 aaaagttgtg gaactaacat tttagggtgg tctgttattt gtttatctag caactgttga
      481 ttaagtatct ctattttccc ataaaaaaac atcatcatac tagttacagc taaccaaatc
      541 cctgtattta ttaaatagcc agctaatatc ttaatactta acggatactg ttttttagtt
      601 ataggtgcaa atttttgctt gcaacttaga aagtatttga ttaaagcata gctagttaag
      661 acaaagaaaa ataagaaaat gtaattactg ttaccgattg ctgattcctt ataagttaga
      721 tacagaacat aaactatcac aaatagtatg gtatggtaaa agccttcaca aaaaaaatct
      781 tcattatttt catcacgcat aaatgctacc attttttcta tcatttattt tttataaact
      841 cctttcacct gctctttcct ataattatag ttacaattaa agttataact ttaactaaag
      901 tagtctttat tcttcttgaa atgctctact aaggcttctc ttataacagc ctttgctccg
      961 ccttgaagtt tgcctttctt ttttcctgct attttgattg cttctttgag tgctggttcc
     1021 attgctactg aataaatcac ttgttcatat tcagcttgtt gtttctcaat ttctttttcg
     1081 tactcttccc attttttgtt ttttgaactt tcttgtaaaa aactttttgg ttgagttagt
     1141 ccatctattt tttttgatac ttctactgct tttttaaatt ctggttttgc catattctta
     1201 ataccccaat tcttctacta gattttgata atattcttgt gcttttaaat atcttttatc
     1261 attgactaac gccaaaggta attcatagat agatactgct tctgaaaagg caattaagtt
     1321 aggtatatat gtttttgtca tatttatatg gtgactatct gctacttctt gtaagttttc
     1381 tagcatgaat ttatgcgttt tggtattcat ctgtgttttt gttggtacaa tatggcgaac
     1441 agttacttgt ttatcatctg taattttcaa cgtggtttca gcatttttaa cagcataagt
     1501 ttctggtgtt gcgacaacaa ctaaatcatc aactatttct acgatatttt ccaaaataat
     1561 atctactgct ggcgccatat caaaaatgat ataatcatac tcttttctga tagggttaat
     1621 gaagtttttt aagtgcttgt ttcgtatttt agggtcttct gatgaagtta gaataatgaa
     1681 gtctgataat gattgattgc taggtattac atctaaaaaa tcattgattt taataaaata
     1741 ttgagaggct tcacttgctg tccagttgct acttagataa gcacctagtg ttgtttgtaa
     1801 atcgtctgaa tccacgccaa aagttaaact ggtatgacct tgagggtctc cgtcaatcag
     1861 taagactttt ttcttttgta acgataaaac ccctgctaca tttgtggctg ttgtcgtttt
     1921 ggcaacccca cctttattat tgtaaaacgc tataatcttt gccataggtt gacctccttc
     1981 gtttatctaa taacaactat accatgttat aactttaatt acaattataa ctacaactaa
     2041 agatttgcaa caaaaaagac taaatatcta gtctttataa attttgtagt tttaccgttg
     2101 gtaggtattg ttcaattgct tttttgagat acttagctat attgcgtttt gaataggctt
     2161 cttgtttagc tctcacataa gagaggtggt ctttgacccc attcaatcct cttaattcct
     2221 ttaactcgtc atagagaggg tacacgtgcg cctgcaatcc tgccataagt gacgtatcgg
     2281 tcatttcata aggacttaat agcatattat ccaacaaaag tttcgtgtat ttacttttta
     2341 atgctttccc tgctaaaagt tcttctgctt gttctttatt ttctttatcc tgcaaataaa
     2401 cagggtcttg ttcttccagc ttgtagaagt catttcgtgc gactggtttc ttcgtgatat
     2461 gaaagacaat agaatcaacg cttcgccctt ttttgacttt ctcgtaagac acgttaaaag
     2521 aggtgtgttg ggtaatttct acaactgcat tttttaatac acggttttca aaactttgaa
     2581 aacgtgagta ttccttaatt gtatcagtca tgatacgcaa atccttaact gtgatagacg
     2641 gattgcggta gctttccact tgttcttctc tccgtccacc cttcacgctg taatgctcgt
     2701 attggttgta attcatagac aaccaacgat ataagataat gctgtacttg ctattaagtt
     2761 cggctatgtc tgacaaagcg tgttgggtaa aattcttttt gagattgact aagtagggga
     2821 taatttcagg actgaaacga attaacactt catcgttata atctgtccac ttgacgtaag
     2881 gaatcggcac aatattttca aattcaaaac catgttcttt tttttctttg attttgaaaa
     2941 aggcttgttt ctgcattttc tcaaccgctt ctttaaaacg actgtgtttg tcattatcag
     3001 acaccttaaa gaaagcgaaa aggtctcttt tagaaagata aaccgtatta tcttccagtt
     3061 gtttttccgt atcaatgcaa gataccgcta actcaaacat tttcaagggc gttttatcca
     3121 ttttggcaat agaggttatc aagctgttat gctccactac tttgcgtttt tcaaggtcat
     3181 tcaaggtcag cacctgcttt tgattttctt gtttttctga tataataggc atgtaaatac
     3241 gctcctttgc gtgttataaa gtagacatag agaaagattc tttgcgggat agttctctat
     3301 gttttttatt gtaccacttt atagcaacac agacaagttt tttgttgcta tatacacagt
     3361 tttttgttgc tatatacaca gttttttgtt gctatataca cagttttttg ttgctataaa
     3421 aatttggata agcctataaa cactggattt tttagcacgc aaaagaaata aaagattaaa
     3481 atatataaga taaatgtaaa taggcttcgc cttttttcac tccgtttaaa aatcttaaaa
     3541 tcaaggtcaa aagaaaaggt caaaatcaaa actgctcaac atggcaacat agaacaatcc
     3601 tacaagctcc tattctgctt tctaagcaac tttacgtgtc attgagtaca aaacatcaaa
     3661 taaacaccta tctttctaaa taccctaact gaaaggcttg tcaacggttt gtgttggcaa
     3721 gccttttggt tatgcttcat tggtctgctc gatgacagga gcagttcgct tgtcaaagcg
     3781 aaagttcttt tctaaaggac taaatgattc cacaatagcc ctagaagccc atattttaca
     3841 ttttaagcgc ctttaaaatg tttctggtaa atttatccat taatagttta acattgctta
     3901 taaggcttta tattggctta tttataaacg tgggggatgt tgacttttat ttttggtgtg
     3961 ttcttgctgg taatctttgt aacggtcaac ggacttgtca tagtaaagca tttctttttc
     4021 gacttgtttt aaggtcttgt gtaagtctgt tcgttccttt tgtaggtttt caattgtttt
     4081 taatattctt gtgtttggtc gtgcataggc tttattttct tcgacatact caagaatttt
     4141 cttatctagg ttttctaaaa tgctttctgt ttggtaatag gtttgttcta attcttttcc
     4201 gatagtttca aaagaagtat caaaatctaa tcctttagaa ataagaaaat caaactcatt
     4261 aaacagttgt tttaacttga ttttggcatt gttagaataa agtggtacgc ttccgctttg
     4321 attttgtagg tttttcgcaa cggtttttcc tagcatgatt ttgttgttct ctggattctt
     4381 actatcaaca aaataaaagc ggtcttgttt tttgagaaaa acttggtaat tgccattttc
     4441 tagtttgtcc acacaacgtg ctggaatttt gattgcggat tttcgttgct ctctttctaa
     4501 accaatgttg atattcacat ataaataatc ggctgtttct ttttctactt gccaatcttc
     4561 tataagcatt tcaatttctg tttcttgttc cttgtcttga atggtttgat tttctgccca
     4621 tagttctttg acttctgatt ctgtataaac cacttcattt tctttcaatc gttccgaaat
     4681 ggattctttc atgaatgctt gattttttag gcttctatct ctgacttttc tttcttgact
     4741 tgtgccactc agttggtaag tggtgtactt tcctttatca tcaacaatca aacctaaagt
     4801 agttgcttta tttttaaagt cgttccaatt gctggcatga gaaagtaaaa atttgatttt
     4861 gctttttatt tcttttcgat aagaattttt agcaaggtag acttgatact ttttgtgaga
     4921 attttttaaa gaacggtcaa taattttgca accatactct ttagaaataa catcagatat
     4981 ttctttcaat tcttctagct ggcttttaga ttgttgaaat tttttgagtg tctttggatt
     5041 tgtagcattc aaaataatat ggttgtgtaa gtgttctttg tcggtatgtg tggcgataac
     5101 aaattctgcg gattcgccta gataggcttt catggttttt aaaccaatct catgcacttg
     5161 ttctggtgtt aaattatctt ctggcgaaaa agattgtaca aaatgaaaac caaatcgttc
     5221 attttctttt ggttgattac cagctaaaat tgccatttct cttgtcagta acatttcatc
     5281 ataggcattg cttggaatgt ttatgttttg tcctgatact aattcaccgt tatttgtttt
     5341 ttgtgggtca gtaatatatt ttaaagcgct agaaagtcct tgtttactgt gtaatgttcc
     5401 tttcgattta ttaggcatgt taatcacagt catggtatta tccctccgtt atttttttca
     5461 acatgtttct taacttttgg tctaaacttt gaatttcttt ttgatactca ttgagtaatt
     5521 ctctggtttg attctgttca gattcattta aatatttggc aatttgattg agattatttc
     5581 caatccgatt gacttctaaa tttgcctgcg caatagtgga cataatttct ttaagagaag
     5641 aaaaatccac cgtataaatt tttttatcta aacaggactt acgagcaaat tctccaaaat
     5701 ttgttaggtt ggcttcctgc attctttttt caataatttt attttctagt tcagaaacaa
     5761 aaaacttttt ttgaatgttt cgttgtctgt ttgctttttt atccatatca aaacctccgt
     5821 aaatctaaaa atagtaattc tatcataata tattatagcg agcttactat gtgtgtacac
     5881 acatagggta aaaagtaatc atcttccact cgtcaaaaac taaaatttac attttagttt
     5941 gttcctcgct tcagacgatt ctttttattt ttggggcgtt gccccaatcc ccattttatt
     6001 agaaatagct aggataattt tcaacatctc cgtacaaact ttcaactgta aaacacaatt
     6061 cttcgacttc attttctgga attttttcaa cgacttcttt tgcttttttg tagtcgttta
     6121 attcgtcttc gtcaattagt tctgacaatt catcaatcaa tttatcaaac ataataagca
     6181 acttttcttt aacaactgga aattcatttg cataagaaat ggcttcaaat atttgtcgtc
     6241 catagtcttc tacttctgcc attatttctt gttcattcat cttttttatt ctccttttcc
     6301 tttgaattta tttttaaagc tgtctaattt actttctttt ttaggtttct ttttttgttt
     6361 ttcttcgtgc ttttttagca attctttttg ttcttttttt gcttcttgaa tttcagtttg
     6421 tagttttgat tttgtaggtt caatattttt tcttttctct aattcaaggt caattttttc
     6481 aattattcct cttgcagttt tttggatatt ttccataact ccttgtaatt cttcgttaga
     6541 aattttatcc aatccccatg aattgagata acccattgta tattttgagg tatcaatatc
     6601 ataattattt gcgacaatat aagcggtaga ttctgcttct aattcttgta aagtatattg
     6661 tttatctccg aaaattgctt ctgattttgt gtgtaatttt gagtgagtga cttcgtgaat
     6721 aagggtatga attaagtctg ctccatgtag gtttgcattg ataaaaatac atttatcttg
     6781 tggggaataa aaaccgtcaa cttctaaggg ttttaatccg atttttactt cgctcacttc
     6841 tgatagagcc ataaataaat cttttgtatt gttagggtct tgattgagat attccacttg
     6901 ttttggtaga ttttcatact cgactagttg cttatcttca aaaactgttc ctagtttaaa
     6961 tcccttgact tctcgaacct tttttccttt ttcgtctagt acaggtttac cattttcgta
     7021 tttatcttta ataattggca taaatatttt atagcctttt gagccttttt ttatatgcaa
     7081 gccttgttct ttccatttcg taaaagtacc aacacgtcct atatccggtt tctgtttaat
     7141 tagaaatcgg atattacggt tagagtatct cggacacttt gcaacgaaat ccaagtaatt
     7201 tttaaacatt tcagaatttt tatattcaac aatttgttct ttcaaaaagc tagaaagttt
     7261 acttgtatct ttttgagcta atagactttg aatatctgtt tgttctttat tatcccccat
     7321 ttttatagtt tcctttctca ataaattatt tggctttagg cttgcccttt tttgtttcgg
     7381 ttgaacagga aaaatccact tgctaaagtt gacaaacttc caataattga caatgcaata
     7441 ttttctgttt ctcctgtttt tggtaaaact ttcatttttc cgtctgaacc tttaaccgtt
     7501 actgttccgt ctttctgaac gtttgcgcca atttctttag cttcaacttt ctttgttgtt
     7561 ccgtctgcta attgaatgat tggtttacta tcttcaactg caacaatcac ttcgcctgta
     7621 tctgtattaa ctgggttttc gattgggttt gtgtctactg gtttacttgg ttctgttggt
     7681 tctgttggtt ccgttggctc cgttggttcc gttggctccg ttggttccgt tggttccgtt
     7741 ggttcagttg gctccgttgg ttccgttggc tccgttggct ccgttggttc ctttggctcc
     7801 gttggttctg ctggctccgt tggctccgtt ggttccgttg gctccgttgg ttctgctggc
     7861 tccgttggtt ctgttggttc cgttggctct ggttgtggtt ctggctgtgg tgctggttgt
     7921 ggctctggtt gtggctctgg ctgtggtgct ggttgtggtg ctggttgtgg ttctggttgt
     7981 ggctctggcg ttgggtttac tgggtcagtt ggtgttggct ctgttgggtc tgctggcgct
     8041 ggttgtgttg gctctgctgt ttttggcaat tcatctgcaa atactgaaac gcttgaaatt
     8101 cctgtgcttc ctaacaacaa cgctgtactg aataatgtaa agatcgcttt ctttttcatg
     8161 ataaattcct cctaagattt tattatttta tttcttgtat tttaggtatt tctttaatct
     8221 caaactcaaa cttttttgat ttgtttttct caatatctaa ttcaacacgc tttgtcttat
     8281 tgatattttc ttctttgact gaaattacat aaattgcctt aaatcctttt ttatctaatt
     8341 taatttcttc ttgcataaca gaaattaaac ttccttgctg gtgttttata tctttggaaa
     8401 tttcatctga tacaaaatca tctaaactaa tcttattttc agaaaaaaga taaggtaaaa
     8461 agtatctaac ataaacgtct atctttcctt tattttcaag taatgtattt tgattatcta
     8521 atactacttt ttgattattt aaactttttt ctaaagttgt aactttattt tccagttttt
     8581 tattagtcac ttcttttgtt tttgagttga cactcattgc aagactagtg attgctaata
     8641 ttaaagaaag gacaattaaa acgttattga cattcaaaga tatttttttg gttcttattt
     8701 cttttttttc tggtttgttt tttgaaaaag gctctaaaat attatcaaaa ctaacgtctt
     8761 tactctgtgg taactctaat ttataagagt agattagaat atcatctttt gtgtactttg
     8821 aaatcaaaac acttttagag cgtaaagaag aagtttttaa tagttgtgca ttagtatcaa
     8881 ttatcaatgt tacaaaatct tcaaaaggaa tactaacaac ggattcatca acaaattttt
     8941 ttatatctgt ttcagaaaaa acatatttac tctgctgttt gttcatcatg agtatttcct
     9001 cgattctgaa tgccaagtaa ctcaatagct tctgtcacgc ttatatcagc ttctttgagc
     9061 ttctgaatta gataatcttt ctcgctatct tcaatcttct tttttagttc tttaagttct
     9121 tgattgattt tatctctttt tttgagtaag tctttttgtt gtttgagtag cccttgttgt
     9181 ctttcttgta aggtttgatt attcatttta aaatctcctt tatcttctct ttttgagtac
     9241 ctgcataggc ttcgttttca tgaatgaaag ttggtacggt atggatattg tattcatcaa
     9301 tatatttgcg atttttttct tgattaagat tgataaactg tacatcatga ttaattaagt
     9361 tgtgcatata gacttgatta aacactttct tacagtcctt acagtcgtca cgataaaaga
     9421 aattgatttt atcctctttt atcgtactga caacggctgt gctactttct gtttggtggc
     9481 ggtcatagac gataaaacct gcaaagagta atagaattaa gaaaaatagt gttgaaccta
     9541 tattcttttt attcattgag cgttcctgca ttctttaaag atacaaattg atttttgact
     9601 gggtcaaact cggctcgata cataaacgtt gcttcttgtg ggtcactgcc tgcaatcgtg
     9661 atagcattat cgacaacgat taagactttc tgctttttat cattcgttgg tgcataaaat
     9721 atttgtggct cgccttttaa tttagaaacg gtttgaacag aagcattgta gcttgttgct
     9781 tcttttggga aaattgtttc taaggcttct tctgtggtaa atttgcgggc tttttctcgt
     9841 ctatctgtta tcgtgtcttt tttcgtatca tagttaaaaa tgcttgagaa tacacctttt
     9901 gtggcaagaa caagctgttc attagcgtta ccgttctttt gttcgtacaa ctcgttatac
     9961 ttgttatcca gttctttatt ttgctttgtg agagtgctta ttttttggtc tttttctcca
    10021 attattttat ttgtagaatt tactaatgtt atataaaaca ccaacatacc tacgaaacag
    10081 aatatacaag caccagatat tattttcata ataaatattt tcttttccat aatttacttt
    10141 ctcccttgaa taaagtaggc ttgttgtgcc gttgctttgt tgatgactct ccaacttctc
    10201 gtcccactgc ttggactaac aacatttgtt tcactgacaa gaaaactgcc gtcatcattg
    10261 acaaattcca cgactgcaat gtgtccgtat tgagggtcac ttcctgcaag cccaccagta
    10321 aagctaaccg cccaaccaac tttaggattg cctttaacta cgttgtaccc tttttctttt
    10381 gcagattgcc accaaaaacc accgtttcca ccattcccac taaaccattc aattggcgtg
    10441 cctaactgtg ccatgcggtt aaaaacgccc catgtacact gtccgaacgg atagttattt
    10501 cctgcatagt ttttattatc gggtgcgggt agtttgagtt tcccctcata ttctttgggt
    10561 ggtttgagtg ttggtgcgga gtttgtacct gtcgcttctt tgcttggtgt ttgattgaca
    10621 acgcaccctt cttctgacaa gccttgacta gcttcttctt tagtaggcat atttggaata
    10681 gctttcttgt ccaacaattg attggattga taaggctttt ctccgtgttt ctcaatcgct
    10741 tctctatcta tccattgata tttttgtccg attgagttgt atgctcctaa taaactaatt
    10801 aagtagttag ggtcagttgc ccaaccacca tttgcgattg ccattaaagt tgcttttccg
    10861 tctgtgttat taatcgctcc tcggtaaagg ctttgatgtg ccataaattc ggctttacca
    10921 actattcctg cgttatagtc tttaaaccac gcatagccac cgcctgtacc gtctccaacg
    10981 tttgtccctg cttttgtgcc tgtcaggtct acggaatcag ccccaaaact tgcaagcgta
    11041 actgggaaat cttctttttt agaagtctta acgcccccca tattgtgggc tttccataat
    11101 gacgtacctt gtggattggt aaagttaaaa ccattttcaa ccattgtttg agctatgctt
    11161 gcgcttggta gaaaaccgcc tgctttccat gagagaatgt aggcttcttt atgacttctg
    11221 ataaaatcgt ctactgattt tggcggtttt gaggtatttt ctgttatggt attgtcgcta
    11281 taactaggtt ttgaacattg gtcatttttt actccgataa ataaagaaat aatcatgata
    11341 accactacta ttgccccaat aatagcaata agaggaatag acaaggtact aacaaaggcg
    11401 attactgctg ttacaataga ttttgtaatg gcaatactat tagcaatggc tttttctaat
    11461 agtatctttg cttggctagc tgtctttgca ttaatactag tagcttgttt gcctgcaaac
    11521 gtggcattct ttttttgtgc tggtctaaag tatttcttaa atcctttttc tgtcttaaaa
    11581 ccatgttttt gcgctagtct tttttctagt ccttgttttg taagaaaacc tactttttta
    11641 gctggttttt ttaatgtttt aactaagtag ctagtggatt gttcttcctc actactaatg
    11701 ttatctggtt caatatcatt aaaagaatta atgataggtt ttgttttttt aactactttt
    11761 ctagttttat aaaaaggttt gcgtggttta agctgtttgc tttttctgtc aattgcctta
    11821 actgacttaa tctttttaag gttcttggta cttttaaacc gtttaaaatc ggctggaata
    11881 ctaggtttct tttttaatac gtatttcttt ttaaagaatt tattattgtc tgtatcatgg
    11941 agggcttttt ttaggttatc attattttta aacctaaatt tttctttaga ttgcttgtca
    12001 ttcatactaa gacctcgcta atctgtctgc atgattttat acagttccgt tgtattaggg
    12061 atagtgtttt caaaaggaac aatggtattg cctgctatta ttagtccact tccttttgct
    12121 ggtgttctta aatattgttc ttgttgttcc gtgatattgt agtgttgttt gatgatttca
    12181 atggcaaatt ttttctgttt gagtagcact aaaaattcag agttgaaaaa cattgcacgt
    12241 ccttcttccg tagctaggac ggtttcaata gattgcgtaa tacctgttgg attccctccg
    12301 tatttccgta ttctcgccca aacatcttta aatttttctc gtgaggattc actagaagca
    12361 ccagagagca agacttgaat ttcatcaaaa taaacccatg tggtaactcc tttagcttta
    12421 ttttctacta cacgttgcca tatcttttct tctatcgcca taaatccaaa cggtttaaat
    12481 ttatttttaa gttgactgat attataaaca attaaacgtg aattaaagtc aacgttcgtt
    12541 tcgtgagcaa atatatccat agagcctgtg atatagagtt ttagcttgta ggataaatct
    12601 cttgcttcgt cattatcaat agtttgtagg acatcaaacc aattgtgaag tgtaggatta
    12661 ctgtactctt tataggttgc aatcgttact tcatcaataa cggactcttg aatttctgtg
    12721 atgttttcaa aaagagaaga aaacagactg acaaggaaac tcgattttaa gccaactggt
    12781 tcatcatcag aatccagtaa attttcatct ggtaaatcta agatattgat atggtggttt
    12841 gtttttggtg ctattttaat caattgaccg tctaaaaggt tagctagctc atcatattca
    12901 ttgtcaggag atagaataat tacttcgtct tgcggatttt ttaagagcgt tgtaatgatt
    12961 tcatattttg cggtcattga tttaccagag ccagaaagcc ctgtaataat accgtttgag
    13021 ttatccatgc tagggtctct acggtttatc gcaatgatat tattagaaaa taagtttatt
    13081 ccatagaatt tacctttttt atggttaata tcaaccgttg taaaagggga attaatagat
    13141 aagttaggcg taattaaatc acgttgaaaa gtcttttcac aatctgtaaa attcattcca
    13201 ataggcaaga tagagtttaa ggcttcttct tgtagatagc ctaaagaaga gaagatagcc
    13261 ccatattttt ccccaatagc tttaataatc cttgtatttt cgtctctttg ttcgattgtt
    13321 tttcctgtaa gatagactaa aaagattgaa gaaaattgct tatcgcctgt ttcacggaca
    13381 aagttgatta cttcatctaa gtcgccctgt gcttcttttg aagtccgtcc aataaaatct
    13441 ggagaataac cctctttaac agcgattttt tgttgtttga ttatttcact ttcaacgctt
    13501 gttgcctgcg ctcgcaagcg tttatttgtt tcaataatag agtagggttg tgcttgaatc
    13561 gtaattacta actcttgtcc gcattctgct aagtctttaa atagattatc ggataattcc
    13621 attggaaaat cctttaagaa aagaatttga ttgaagcggt cgtctatttc tacccctgtt
    13681 ttactaaatt taagggaact aggagcgatt aaatccttag ttgtaagcat agagagtctt
    13741 atatcgttaa aatttccata taaaggctta tcataattaa gcattttatt catcacagaa
    13801 agacgttcaa gcccatctaa tggcttaata ggaacttcga tagaagctag cgtgcttgac
    13861 atcaagtttc ttgtgttttc tagcttgcct aaagcctttt tcttgtcctc tgcattggta
    13921 gaaatagtaa tgtatctatc aatctgatag ttgtttagcc ctttttgata gttaaggtta
    13981 atcaactcat ttaattcttc tcttaaatag tcgtatgaat cgccctgtaa aggaaattca
    14041 tttttttctt gatactcttt ttcgtgtact ttagaaacta gtaatgttaa ttggaaatgt
    14101 tcgttatcag ataacgtatt tatcattgtg ttgtattttt gaataatatt tgttttttct
    14161 tcgtttggcg cggtcgcata atttgtagaa cctaattcat aagtcattga ataattgtca
    14221 tcaacaatat tcatcagtcc attttcaaaa agagaggtat atcttagtgt gttttgactg
    14281 gaactacttt ttgctttttg cgcattcttt ttcaatcggt ttaagcgtag cttttcagtc
    14341 ttcgataaag gtgtctgttt ctttgacttt ttttgattga ataaactctt tacgttcata
    14401 ttgcactatc ctctctgttt gatatgttct ttttgtaggc ttctttaaat aattccattt
    14461 cagtttgaac cagcgatcgc cagttagtcc gtttattttt actaaagaaa cgaaaaaact
    14521 aataaataga atcaacatta gtggaaactc aaagaaaaga ctactaatgt gtaggaaaaa
    14581 attatttatc atgattaagc cagtaacacc agctatactt aataagaaaa tgaattgacg
    14641 gaacgttaaa ccagctacct tgcttttaat ttttgaaccg tctttatata ccttgatata
    14701 catactaacc tcctaatcca acgacagatt ttgcatattt ttcagactgc aaaattaaga
    14761 cagcaacgat aataggttga attaaagaaa cgaatccccc agcaactgct ccttttgggt
    14821 ctggtgctga aaatgaatta cttaatccta aaattatgag aatgacaaat ccttgtagcg
    14881 ccttagcaca gtattgcttg gcgaagccta ctccaattga gcgatattca ctagaagcaa
    14941 atgaagaaaa tgcaataggt gcgagtatgg tatacacgta tagttggaac gttcttagtg
    15001 aaactactac tacaatgatt atagaagccc ctgcccctac taagtaagtt attaaccaag
    15061 atataacttt tccaataccg tccgtccaag aaacaccttg aagtgaagct ggtgcaaaaa
    15121 tattatacga accagtatct ttaattaaat taatcccacc aactacaatt tcattaataa
    15181 aaggtagaat aaacactgaa ccagctacaa gcgcccctgc aagtagagca ttgacagaag
    15241 cttgaaacat cgttattgga acaacagtac cctcattatt tttatttgcg gtttcaaaga
    15301 ttttagctat ctcaaaaatc aaaaagaaga taagaagata aattccaact ttagttgttg
    15361 agttctgcat gacttgtgtt acatagctcc agccattctc atttaatttg tctgcatgtc
    15421 ctccaagcac tttgaataaa tccatagaat aaacctcctt atttctattt gcttggttaa
    15481 tatccttaaa ttaaacgccc ggaggatttg taagtaagtt gaccaatcca gcagccccac
    15541 ctaaaagcaa accaccaagt ataagcataa ttcctcttgt ccgttgtcct ccgttgcttt
    15601 ctgccatact ttcggcaatt tgaatcactc ctacgataat taagccaatc gaacctgcac
    15661 caaaaaacca cgttaaaagt cttttaatag tgtctaatcc atttgcctgt gctaaaacag
    15721 ggtgtgttaa tgcagtaaat gctaagtttg ataatgttgt tttcataatt tattcctcca
    15781 ttgtattcgt taatgtttct actggtttct tgctattcca atagtcaaat gccttttcta
    15841 attcatcaac cttaaattct ggttgaaatt cataccagtt atcgtctgac ggactactgc
    15901 tccattcagt tgaacgttta tgccctttaa aaggaaattt tttccctcga tacatcggca
    15961 ttgaagagat agagactaaa cattcacttc taggcaatct atttacttcg tctttggtta
    16021 ttaaatctct tcctagaacc tcatgactaa ttgaatatga accattagaa cctctatttt
    16081 cagagttctt tttcatgttg attgtttctt taccagcacg ttcagagaag aatttttctg
    16141 tttctggttc tgttccccct gaaaggtaca tgattgtatc gcagtttccg acaaaacttt
    16201 tccaatcttc tttatacata gaagttaatt gattaatatt ttgtacgata acgttaatac
    16261 tcatttcacg tgaacggaaa acagctaatg cttctttgat gtttggtatc tttcctaagt
    16321 ttgcaaattc atctaataag aaacgaatat gattaggtgg atttccgtga aattcattat
    16381 ctatttgatg ttcaagtgtt ctaaacgcta agacaaatat catggtggtt aagaaattaa
    16441 aggtggtatc catatctgga atcggtagat agacgatagt ttttttagta acccaagtat
    16501 caatttctag ttcatcatca tctaatatgt ctcttatatc ttgtaagtcg aacattgaaa
    16561 atctagcagt agcaatcgct ataacactac ttcttgtatc tccacgataa tttttaaaac
    16621 tattgaattg agtaacagca taattttcag aaccgaaacg gtcttcaaat ttttcaaaca
    16681 ttctctctaa aacagacggt acttcttcgt tttccctatt caataatcta attaaatccg
    16741 atacttcata cagcttaggt aaatctcctg tacctgtaat tcctttttta gggtcgcctt
    16801 tatatttgta gtaaagaaat gaaaagattg aaccaagtag caattcttct gacttatccc
    16861 aaaaaggctc accttttcga ctgttttcgc catttgtagc gtctataata cattgaataa
    16921 ctcgctttag agtgatttca tcatgaatat atttaaaggg attaaactta ttgcaatttt
    16981 tgaatgtatt aacatcaaat atctttactt gatagccatt ttcagacatc atcttcccag
    17041 tttcatggaa cacttttcct ttagggtcag ttagtacata agaggaatgt agttgtgata
    17101 tattgggttt cacatacatc tctgttttac cagcaccact acctgcacag acaagaacgt
    17161 tcttatttct ttgataatca aaatccatat tctttgttgt caaactcatt tttacatctt
    17221 ctgaaaaaat aagattgtta tcttcgtttt tgctgtccgc aaatttcttc aattctttac
    17281 tcgttgccca acgagccgaa ccatactctt taccttgtct gtatttccca ttacttgtac
    17341 ttaacataaa gaatagtaaa gctaagacca gacctaaaag gaatgccaat aaagggtagc
    17401 ctttaaaact aaagaatagc cattgattag aataggaatc cattgtgttt gctaaactat
    17461 taaatatttt ttgtatgccc tctgctttct catttaaaag aaaataagca aagcgattaa
    17521 ggaaacaacc aagtatcaag cctaagactg aaaagattat gattttagct ttaactacct
    17581 ttgataaatc tttcactaga tactcttccc tttccctaat gttttgttga ctgccttatc
    17641 cgcaacattt tttagcattt cctgttgttc ttgttttgca attttaattt catcttttaa
    17701 cgaaggttct tttttgcctt tgtctggttg gatagctttt tcttttttga gatatttttg
    17761 aggattggct tgcatatcac gcattacttt ttcagttgta gctaaaatca tatttctgtc
    17821 tttagcatga aatagtaact ctgtcttgcc gtctggcaat tcacgtgata gaaaatcaac
    17881 gccattcttt ttgtattctc tgtgtaaggc tttgatagca acattactgt tggataaact
    17941 ttcatttaat aactcgctat ccgtgaagtt aggaacttta cctttgttat cgtaatgctt
    18001 tttcaataag ctgtaagcat gtctgatact gctttttcca acccctaaaa ttaaccttac
    18061 tgtttcatct tcttctgcca taaattattc tcctcctgaa cgtagctctt ttggtataaa
    18121 ccatactaaa aagctgataa ctacctcgat aagaagtaca attgctagtg gcataatgta
    18181 ccaattccac attcctgcga aagacatttt tgtctgcaat acttcttgta cataacttgg
    18241 atattttgaa atatcaaaat aacttgcagg taaaaattga tgtgctgtta caagcgctac
    18301 aacttgtccg taaatccata taattattgc ccctgtaatt aaattttgaa taagaaataa
    18361 tggtgatatt agaacaaacc taattatcaa tccacttatt cctactaaaa tctcaatagg
    18421 tcgaataaaa attaaatgga gtatcgaaag aaatttattc atatttcttc atccccccct
    18481 atccaaaatg caaaaatagt cctaacattt ctatgacttt atagaaatga ctttctaagg
    18541 tggcatgcgt gttaagactt ctaattaatt gctattttga tataatgatt tgttgggtaa
    18601 aaacaaagaa tatgctttgt tttgtagacc gctttaagag gtaggattct taggcggtcg
    18661 cccgcttttt ttacgtgctt caatacttca tcaatcccac gttgattgat aactaaatta
    18721 tcgcccactt tttttaattc tacatgttca aaatattcag ggtgattagc tttgattgaa
    18781 gaaaaataag cacgacttct tccttgcttt atactggctt ctgataaact ataccatttt
    18841 tcatcatgca ctcttgttcc tcctttctta attataattg tactctttta ttttacagct
    18901 gtcaaatatt ttaaacgtta taataaaggt ctaaatagat ttatttgtaa ttatctgact
    18961 atttcgcttt gacaagcgaa ctgctcctgt catcgagcag accaatgaag cctaaccaaa
    19021 aggcttgcca acacaaaccg ttgacaagcc ttttggttag ggatattttt ttattttctt
    19081 ttaataccat actttgttaa atatcgtttt agtgtactga ctggaatttt agtatgatgt
    19141 tcaatttctt ttaaagtata ttcttttgat tgatatagat taaacgctct ttgtagtaaa
    19201 gggtcgtctt tttgaaaggt actttttctt ccagtaaatc gtcctttttt ctttgctaac
    19261 tcaatccctt gtgcctgcct ttccttaatt cttttccgtt cactttccgc ttgatatttg
    19321 tataattcaa cgactaaatt attaattaat cgtctcaaat tttcgtcttc aatcccattc
    19381 attgagggca agtttaagac ttccagcgta gctccttttt gttgaatggc attcataatc
    19441 tctgtaaggt ctttattgtt ccgccctaaa cggtctaact ccgttacaat gacaatatcc
    19501 ccctcacgta gatagtccag catggcttgt aattgaggac gttcggtgga ttgtccgctt
    19561 gctttatctg aaaagacctt agaaacgctc tgtaacgctt ctaattgtct atctaggttt
    19621 tgttctttgc tacttactcg tgcataccct attttactca taaaccacac tcctaatttt
    19681 ggcaaactct aatgagcctt tacaaatctt attatatcag aaaaacaaaa aagcccacta
    19741 gagtgtactc taatgagctt aaaataaata tttcatggct ta
//
