LOCUS       NZ_CP110046             3265 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Enterococcus faecalis strain BE43 plasmid pBE43_5, complete
            sequence.
ACCESSION   NZ_CP110046
VERSION     NZ_CP110046.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31342466
            Assembly: GCF_025958125.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Gaeta,C., Pinzauti,D., Marruganti,C., Bollini,R., Fabbro,A.,
            Santoro,F., Grandini,S. and Pozzi,G.
  TITLE     The presence of Enterococcus faecalis in saliva as a risk factor for
            endodontic infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Pinzauti,D. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2022) Dipartimento di Biotecnologie Mediche,
            University of Siena, viale Bracci 16, Siena 53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology :: Oxford Nanopore GridION, Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:49:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,911
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 35 of 99
            Pseudo Genes (incomplete)         :: 57 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 18 of 99
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP110046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BE43"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pBE43_5"
                     /geo_loc_name="Italy: Tuscany, Siena"
                     /collection_date="2019-09-16"
     gene            complement(join(2874..3265,1..856))
                     /gene="mobV"
                     /locus_tag="OLM01_RS14880"
                     /old_locus_tag="OLM01_14880"
     CDS             complement(join(2874..3265,1..856))
                     /gene="mobV"
                     /locus_tag="OLM01_RS14880"
                     /old_locus_tag="OLM01_14880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_264526526.1"
                     /translation="MAHLKKNTRASLSGLSIHFERKTDNHSNKEIDISRSHLNQDLMQD
                     NSNMVERFNERLEDVYCMNRKDVKALGTWVVTLPDELKDLSHDKQQEFFNETKNFLDKR
                     YGKENAVAAIVHYDETTPHLHYGFVPVVYDEKKDRLKVSAKEVLNRKDLQTFHDDLNKH
                     LKEKLPFYEKGILNDQTLPFQNVEEIKKYNETYQEMKQTLEQKKAVIRMKNDALEPFEK
                     SLKRINDTEKMFDTIDKNAKKGFGQVVIKSSDFEKLKTQAVENQVLANANEKEMYILKD
                     KNKKLEKDNRMLSTKLDHKTQKNEALTKKNNELTKVKNDGHKFRELVKRDLEKQYNIVD
                     LTEKEVNALSVVKAIDEDIKPKNKEEGKSWLDDLNNGKGTRIEESMLEKALEKALELYR
                     EIVQKVERMMGMNISL"
                     /name="mobV"
     gene            complement(1167..1385)
                     /locus_tag="OLM01_RS14885"
                     /old_locus_tag="OLM01_14885"
     CDS             complement(1167..1385)
                     /locus_tag="OLM01_RS14885"
                     /old_locus_tag="OLM01_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025480940.1"
                     /translation="MKDVQDLFKEYYDSHNLEKNSQYADFSKEQLVIEAEYLHDSLTRI
                     LKYINDGGTDINKIYAEVMDGIYESRI"
                     /name="hypothetical protein"
     gene            complement(1399..2370)
                     /locus_tag="OLM01_RS14890"
                     /old_locus_tag="OLM01_14890"
     CDS             complement(1399..2370)
                     /locus_tag="OLM01_RS14890"
                     /old_locus_tag="OLM01_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_195640501.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNVQSLNTPLKIQWSIDRITIVGT
                     LNENIYYHTQNDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKFHE
                     GKGRIDFNPNKISQFLASSMKRFIHDLFLDPHFSRADIACDIVDVPDDFITQYRVVDPV
                     SFKPIYGRSGQLETAYWGSRSSERQIRMYNKKLEQERKRKIVPKEIKTWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHFLPMDTKATDRIMLAGLLTDQNFWSLLSRNSKYKYRELL
                     KQESQNDELTNHLRETFFESADILKEELDTWLQGLDVTEEKE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(1404..2288)
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.157"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 attttttatt tttatctttt aaaatataca tctctttttc attcgcattc gcaagaacct
       61 gattttctac tgcctgcgtt tttaattttt caaaatcact tgattttata acgacttgac
      121 caaatccctt tttagcattt ttatcaatcg tatcaaacat tttttctgta tcattgattc
      181 tttttaatga tttttcaaaa ggttctaacg catcattttt cattctgatg actgcttttt
      241 tttgctctaa tgtttgtttc atttcttgat atgtttcgtt gtattttttt atttcttcaa
      301 cattttgaaa aggcaatgtt tggtcattca aaattccctt ttcataaaaa ggcaacttct
      361 ctttcaaatg cttgtttaaa tcatcatgaa atgtctgtaa atcttttctg tttaagactt
      421 cttttgcaga tacttttaat ctatctttct tttcatcata aaccaccgga acaaagccat
      481 aatgtaaatg aggagtagtt tcgtcatagt gtacaatcgc agcgacagca ttttcttttc
      541 cgtatcgttt atctaaaaaa tttttcgttt cgttaaaaaa ttcttgttgc ttgtcatgag
      601 ataaatcttt taattcatca ggcaacgtta caacccaagt tcctaatgcc ttaacatctt
      661 ttcgattcat acaataaaca tcttctagtc tttcattaaa acgttcgacc atatttgagt
      721 tgtcctgcat taaatcttga ttcaaatgtg agcgactaat atctatttct ttgtttgaat
      781 gattatcagt tttgcgctcg aaatgaattg ataaaccact aagagaagca cgagtatttt
      841 tctttaaatg agccatacaa tatccctcca attcacaagg tctttagaac cagtataacc
      901 tagtttaaaa tctggtatag cctgttatac caaatttcat ttggacaggc tctgccgaga
      961 gctattcgcc tgcaagcagt cgagctaaaa cgttcagaat aaatcgcctt taggcaattt
     1021 atgatcttct cttttttagc cttacagcaa tcaaaatttt ttcaaatttt cattcgctgc
     1081 ttttatattt tcggagttgg cgagattgcc attttccgaa aatataaaaa aggagtaatc
     1141 gccttttgac gattactcct aaaaagttat atccgacttt catatattcc gtccataact
     1201 tcagcatata ttttatttat atcagttcca ccatcattta tatatttcaa aatacgagtt
     1261 aaagaatcat gaagatattc tgcttcaatc actaattgtt ctttgctaaa atctgcatac
     1321 tgactatttt tttctaaatt atggctatca taatattcct taaataaatc ttgtacatct
     1381 ttcataaaaa tatccccttt actctttttc ttctgtaaca tctaaacctt gcaaccacgt
     1441 atctaattcc tctttcaaaa tatctgccga ttcgaaaaaa gtttctcgta aatgattcgt
     1501 caactcgtca ttctgacttt cctgttttag taattcacga tatttatatt ttgaatttct
     1561 agacaaaagc gaccaaaaat tttggtcagt taacaaacca gcaagcatta ttctatctgt
     1621 cgctttcgta tccattggca aaaaatgcgg actagcaaaa ctatccaagc tttcttgaac
     1681 cattgcatgc caatcagtag cttttcctct tcgcaactgt aattctagtc gccaccaggt
     1741 cttgatttct tttggaacaa ttttccgttt tctttcttgt tctagctttt tgttatacat
     1801 gcgaatttga cgttccgaag accgagaacc ccaatatgcc gtctctaatt gaccactacg
     1861 accataaata ggtttaaaag aaactgggtc aaccacacga tattgggtaa taaaatcatc
     1921 tggcacgtcc acaatatcac aggcaatatc tgctcgggaa aagtgcggat ctaaaaatag
     1981 gtcatgaata aaccgtttca tagaactagc taaaaattgg ctaatcttat tcggattaaa
     2041 gtcaatccgc cccttaccct catgaaattt aagtatctca atataggcta tattttcctc
     2101 gtactggtct aacagttgcc aaccattatt cccaacagcc tttagatatc cattgccttc
     2161 attcaagcgc ataagctgtt caaaatctaa aatcaacaca tcattttgag tatgataata
     2221 aatattttca ttcaaagttc caacgattgt aattcggtca attgaccact gtatttttag
     2281 gggggtgttc aaactttgaa catttaaccg cctgttagat atgcggttaa gtttttccca
     2341 ctcatttatg atcgtgccac gtttactcaa attttgttcc tccttgaaga aaaaagagat
     2401 tggtggaaag aacttttcag agttcacgta actctaaaaa attcataacc aacaatctct
     2461 agtcttgaag aaacctaaaa atattcgtat aatgaatttg agttacggga ctagtagaga
     2521 tagtttccgt aagagtcgag aacgttggta gcgttgctcg actatttttt tgctcttttt
     2581 cctgtttttt ttagtatatc acttaaattt gaaaagataa aagaagtact aaaaataaat
     2641 ataaatcatg agggaatttc attccctcaa actcccttga gcctctccta ccgaaataaa
     2701 ggacgctatc gcttatgatt tcgttcagtt atcgactaat gcaatctaac acgaccgttc
     2761 cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta acgattgcgc
     2821 cgataactga acgaaataaa cgctaaaaca ccttagaacg cattttatga gctttataaa
     2881 gatatattca tacccatcat acgttccact ttttgaacaa tctctctata taattcaagt
     2941 gctttttcaa gtgctttttc aagcattgat tcttctattc tcgtgccttt cccattgttc
     3001 aaatcatcta accagctttt accctcttct ttattttttg gcttaatatc ttcatcaatc
     3061 gcttttacaa cagacaaagc attcacttct ttttctgtta aatcaactat attgtattgt
     3121 ttttctaaat ctcttttcac caattctcta aatttatgac catcattttt aacttttgtt
     3181 aattcattat tttttttagt taatgcttca tttttttgtg ttttatgatc tagtttagta
     3241 gacaacatac gattatcttt ttcta
//
