LOCUS       NZ_CP109991             1283 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP424 plasmid pKP424-9, complete
            sequence.
ACCESSION   NZ_CP109991
VERSION     NZ_CP109991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31413558
            Assembly: GCF_025946965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   He,F.
  TITLE     Global emergence of carbapenem-resistant Klebsiella pneumoniae
            co-carrying multiple carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2022) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,643
            CDSs (with protein)               :: 5,643
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 72 of 183
            Pseudo Genes (incomplete)         :: 116 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 34 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP424"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP424-9"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-10-28"
                     /collected_by="Fang He"
     gene            join(1192..1283,1..778)
                     /locus_tag="OJ961_RS29715"
                     /old_locus_tag="OJ961_29720"
     CDS             join(1192..1283,1..778)
                     /locus_tag="OJ961_RS29715"
                     /old_locus_tag="OJ961_29720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasyl DNA replicon protein arp"
                     /protein_id="WP_180398993.1"
                     /translation="MLLNKDRFGKLTSKSLDSIAALGLREANAELPCLFRNNSTEISLK
                     TPPEPLSPLAFELSTNLKKSASALAWNVQYFVDTYGLSNVGFLTLTFRDHVTDPKEAQR
                     RFNSLKTHILAKRYRAYIRVMEPMKSGRIHYHLLVALHSDIRTGFDFPAVYRQDYSSAN
                     KAIRSEWSFWRKTAPKYGFGRTELMPVRSNSEGIGRYVGKYISKGIESRTEQFKGVRLV
                     EYSRKAKIASTRFQFVSDGSYEWRRKLSIFVHYIADNMGCEPSFDGLRRVLGSRWSYHW
                     RDFIMNIE"
                     /name="phasyl DNA replicon protein arp"
ORIGIN
        1 tccttgtcta tttagaaaca actcaaccga gatttcttta aaaacacctc cagaaccctt
       61 atcacctctg gcttttgagc tttctacaaa cctcaaaaaa tctgcctcgg ctctggcatg
      121 gaatgtacag tattttgtcg atacctacgg cctttcaaac gttggttttt taaccctgac
      181 ttttcgtgac catgtgaccg accccaaaga ggcgcagcgc cgttttaact ccctcaaaac
      241 acatatcctt gccaaaagat accgcgctta catccgtgtt atggagccta tgaagtcagg
      301 gcgcatccac tatcacttac tcgttgcact gcactccgac attcgtaccg gattcgattt
      361 ccccgctgta tatcgccagg actactcctc cgcaaacaag gctatccgct ccgagtggtc
      421 attctggcgc aagactgcgc cgaaatatgg cttcggtcgc actgaattga tgcccgttcg
      481 gtcaaattct gaggggatag ggcgctatgt aggcaaatac atttctaagg gcatagaatc
      541 gcgtacagag caatttaagg gcgttaggct ggtagagtat tcccgcaagg ctaaaatcgc
      601 ttctacgcgc ttccagttcg tttctgacgg gtcttatgag tggcgtcgca aactttctat
      661 cttcgttcac tacatcgcgg ataacatggg ttgtgaacca tcattcgacg gcttacgccg
      721 tgtccttggc tctcgttggt cttatcactg gcgtgatttc atcatgaaca tcgaatagtc
      781 cacgctgttt aatagtggac tcttgttcca atatggaaca ctttccgcct cacgcagcca
      841 gattgtaaat ctgtcagcgt tcaccgttgc tgaactcgtc aaatgctaaa tcgggggtcc
      901 ctttagggtt ccgatttagt gtttgtacga caccccgatt aaagacactt cttgctggtg
      961 tgtgttacgt gcgcagcagg tgacacacac ctgtaagacg ggttttaagg ggttccgacc
     1021 actgactaat ccgtaacacc atgagagacc cccgtttgac cacgttgccg aatcgctgta
     1081 acgttgcccc gatggctaaa aagtaaaacc cccgatgcgc aaacatcagg ggtctttagt
     1141 cagtaagcat ttctagggct cattctgtac tttacttaac tggacaactg gatgttactc
     1201 aacaaagacc gctttggcaa actcacgtca aaatccctcg attcgattgc tgctctcgga
     1261 ctccgcgagg cgaatgccga gct
//
