LOCUS       NZ_CP109990             7329 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP424 plasmid pKPC-2-KP424, complete
            sequence.
ACCESSION   NZ_CP109990
VERSION     NZ_CP109990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31413558
            Assembly: GCF_025946965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   He,F.
  TITLE     Global emergence of carbapenem-resistant Klebsiella pneumoniae
            co-carrying multiple carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2022) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,643
            CDSs (with protein)               :: 5,643
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 72 of 183
            Pseudo Genes (incomplete)         :: 116 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 34 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109990.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP424"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPC-2-KP424"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-10-28"
                     /collected_by="Fang He"
     gene            complement(join(6933..7329,1..308))
                     /locus_tag="OJ961_RS29665"
                     /old_locus_tag="OJ961_29670"
     CDS             complement(join(6933..7329,1..308))
                     /locus_tag="OJ961_RS29665"
                     /old_locus_tag="OJ961_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <374..952
                     /locus_tag="OJ961_RS29670"
                     /old_locus_tag="OJ961_29675"
                     /pseudo=""
     CDS             <374..952
                     /locus_tag="OJ961_RS29670"
                     /old_locus_tag="OJ961_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            1486..1764
                     /locus_tag="OJ961_RS29675"
                     /old_locus_tag="OJ961_29680"
     CDS             1486..1764
                     /locus_tag="OJ961_RS29675"
                     /old_locus_tag="OJ961_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213990.1"
                     /translation="MIHTANRTFHQLYREWIRERREHMHNVLTWERDRYGARLVGLFYR
                     YCKVANPFPRCTLNTRINYRAHAVNLPDWPARSLELNKMWLSWREKK"
                     /name="hypothetical protein"
     gene            1875..2300
                     /locus_tag="OJ961_RS29680"
                     /old_locus_tag="OJ961_29685"
     CDS             1875..2300
                     /locus_tag="OJ961_RS29680"
                     /old_locus_tag="OJ961_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_013213989.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKGL
                     CREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYSHL
                     SFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
                     /name="antirestriction protein"
     gene            2428..2583
                     /locus_tag="OJ961_RS29685"
                     /old_locus_tag="OJ961_29690"
     CDS             2428..2583
                     /locus_tag="OJ961_RS29685"
                     /old_locus_tag="OJ961_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013213988.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYREA
                     AATVTH"
                     /name="hypothetical protein"
     gene            2629..2925
                     /locus_tag="OJ961_RS29690"
                     /old_locus_tag="OJ961_29695"
     CDS             2629..2925
                     /locus_tag="OJ961_RS29690"
                     /old_locus_tag="OJ961_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_013213987.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDVA
                     DLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
                     /name="transcriptional regulator"
     gene            <3029..3910
                     /locus_tag="OJ961_RS29695"
                     /old_locus_tag="OJ961_29700"
                     /pseudo=""
     CDS             <3029..3910
                     /locus_tag="OJ961_RS29695"
                     /old_locus_tag="OJ961_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(4160..5041)
                     /locus_tag="OJ961_RS29700"
                     /old_locus_tag="OJ961_29705"
     CDS             complement(4160..5041)
                     /locus_tag="OJ961_RS29700"
                     /old_locus_tag="OJ961_29705"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(5317..6297)
                     /locus_tag="OJ961_RS29705"
                     /old_locus_tag="OJ961_29710"
     CDS             complement(5317..6297)
                     /locus_tag="OJ961_RS29705"
                     /old_locus_tag="OJ961_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="WP_013213985.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQN
                     RESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGLGR
                     CLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAID
                     RATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKKKEPS
                     GTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNY
                     VKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
                     /name="IS481-like element ISKpn27 family transposase"
     gene            complement(6420..>6881)
                     /locus_tag="OJ961_RS29710"
                     /old_locus_tag="OJ961_29715"
                     /pseudo=""
     CDS             complement(6420..>6881)
                     /locus_tag="OJ961_RS29710"
                     /old_locus_tag="OJ961_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     ARG             complement(4164..5041)
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          393..952
                     /name="IncU"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgacagtgc ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc
       61 ttcacgtagg tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag
      121 cgcagccgtt tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga
      181 tcgacattca ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat
      241 ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat
      301 gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
      361 gcaacagtgc cgaatcaacg aaggcgagcg aatgaggcat cttttctggc gtcttaactt
      421 tctccttctc tccggggcaa caaacagcgc ccagatgtac catgaggccg ccgctctttc
      481 ctccgagctg gatactcgtt ggtcttaccg ctccaaagag cttatgacgc tctacagcaa
      541 ggcgaaatcc tatgaggctg gggaacgtgt cgagttcggc ggaaagtcgt ttgcaccgct
      601 atacacgccc aaaaacgaca cgctgatcaa cctattccag atctcacagc aagaacaaga
      661 gcaactgaag acgattatca gcactgacga ggctcagaag cgccgcagag agcgcgatag
      721 actgcgtgat gaagaacgtc gccgcgccgc tggccagctt gagcgtgaag cctatgaagc
      781 caactcattg agcaggcaaa agccctggga agctatgggc atgagccggg ctaaatggta
      841 tcgcctgggc aagccttcac ctcaacaaaa cagtgagaca agtccctccc ctattactaa
      901 tggcgaagcc tcagcggttg cccttcaggc ggccaggtgt aaagccgttt agatggctat
      961 ttcaaagtga gtgagatttc gtaacggatg gcagccctag aggctgcttt cttttgaggg
     1021 gttagcaggc gtatagccat gctggatctc agtctatcac tacctgctta atattccacc
     1081 tttagatccg gcagcagttc gaccaggtgg ccaacttacc aggcaccggc agcagctcga
     1141 ccgggtggcc aagttcagat ctggacgcca gaaggaaatc aaccaggtgg ccaacttacc
     1201 aggcaccggc agcagctcga ccaggtggcc aagttcagat ctggacgcca gaaggaaatc
     1261 aaccaggtgg ccaactcacc aggcatcggc agcagctcga ccaggtggcc catttcgtac
     1321 gtacgaaatc aggcgacgct atgcgaaatc ttggcggcaa tcccccgcga ctacgtgatt
     1381 tttttaacgt agagcgcgct ttttcttgcc atagtaggcc attttggcct ataatcttaa
     1441 ttaagaagac cgggcacccg ccacggtcaa taccggagaa ctccgatgat ccacacagct
     1501 aaccgcacct ttcaccaact ctatcgagaa tggatacgcg aacgccgcga gcatatgcac
     1561 aacgttttga cctgggagcg tgatcgttac ggtgctcgcc tcgtcggtct gttttatcgt
     1621 tactgcaagg tggccaaccc ctttccccgt tgcactctga atactcggat caactaccgc
     1681 gcccatgcgg tgaatctgcc agattggcca gcccgcagcc tggagctgaa taaaatgtgg
     1741 ctcagttgga gagaaaaaaa gtaggccgat ttggcctatt ttttattgta cccgcttgac
     1801 acaataggcc aaaatggcct attatatatc tcagaaggcc gggcacccgc cacggtcaat
     1861 accggagaac tccgatgatg caaacagaac ttaatcccct gatctgctcc ctggtcgcca
     1921 cccctcgccg tatggctgca atgccccgct atgtaggccg tttctatgtg gttttcgagt
     1981 cgatgcttta ccagcaaatg aaagggctct gccgcgagta tcgcggggct tattggctta
     2041 tgtgggagct gtcgaacggc ggcttttata tggctcctgg gcgtcgtgat gaaatgctga
     2101 acatcgaggc catgaactac tttagtggcc aaatgagcgc cgatgctgcc gggattaccg
     2161 cttgcctcta cctctatagc catctgtcat ttcacacaga aggcgctgac caggagcggt
     2221 tttcaaggct gtatcacagc ttgcgggatt gggcttgtga gcatgacgaa aaagaggcca
     2281 tcttggccgc aatagactag caacagaaag ccccggtgat ggccggggct cttaaaggag
     2341 gtgcataggt agaaaaagaa atggtgcccc tggtatggct gcaaccaatc acaggggctt
     2401 tcaccggtca atttgaggat aaacgcgatg aatacggata ttgtatcact tgcaaaacac
     2461 attggcgacg ctctgacggc ccgccgcccc gttcatatgc cgcgcatgaa gatgcgggag
     2521 cttggcttgc tcttgattga actgaaggcg tatcgggagg ccgccgcgac cgttacccat
     2581 tgataaaaaa gccccggcat tgaccggggc tatttgagga tttatcgcat gattagacct
     2641 gaaacgctta ggccgttcgc tgaagattgg caggctccaa ccgccgatga aatcaaagag
     2701 gtgttggagc tgattaggca acgaaaaggg cttagtaagc ctctcagtgg cgttgatgtc
     2761 gctgatctcg ttggtttgcc tggtgagaga ggcagcggaa agggaacccg aaccttcagg
     2821 cgttgggtga gcaaaaccaa ccctagccct attgcttatg gtgcctggtc tatcttggcc
     2881 cacctggcag gtttcggggc tatatgggac gctgaccggg attgaaacca tgaccgaaca
     2941 taaggccgaa cgtgctccct ggggagactt cccggcagta gtgaggaacg gcgatctaaa
     3001 ggatctctct aaagaacctg aatacgaagc cgccaaacat ggcgaccaca aggcgatgag
     3061 ctataagcgc atgaagccgg ccgaggacga attgcattgc gagatcaagg cgctgcttga
     3121 tcgcgccaag gctaccgacg accaggagcg taacgagccg gagctggaca ttcctgccga
     3181 gatttctcgc cgcgagaagc gcctggaggc gatccaggcg gcaaaggcgc gcctggaagc
     3241 gcgccagcgt gaagcggacc aggcccgggg gcgcagcgaa gacgatggcc gccggcctcg
     3301 ccatccggat ggctcggaca agggcggtgg ctcgtacaaa cgcgagtttg gtgtgccgga
     3361 tgaccgtgat caggaaagct tcaccgatcc ggacagccgg atcatgaaac acgccggtgg
     3421 tggctccgag cagagctaca acgggtacac agcggtcgat gccgagcacc agatcatcgt
     3481 ggcggcggag ttgaccaact gcgccgcgga cagtcaggcg ctgctgggca tgctggcagc
     3541 agttcaggcc aacaccggag aaatgccggc ccagacgctg gcggatgcgg gattccgtag
     3601 tgaggctgtt ctggcaaagg tcgccgatca ccacggcgat gtcatcgttg ccctcggccg
     3661 cgagggacgt gaagatgcca aggtcaatgc caagacccat ccgcatacgg cggcgattgc
     3721 ggcgaaattg aaaacggagc aaggcgatgc agcttaccgc cggcgcaagt cgatcgtgga
     3781 ggctccgaat ggttggatca aggcggtgat gggattgcgt cagttcagca tgaggggcct
     3841 ggacaaggtg caagccgagt ggaagctcgt ctgcatggcg ctaaatctga ggcgaatggc
     3901 gtatctgtga gggcgaaggt taaatggggc ggctcaaatg caccccagtc gtcataacac
     3961 gccgcgcgcc gcaagattgg tatccttggc gccgacgcct tgccaattgc agagagcgcc
     4021 gccgccatcg tcagtgctct acagaaaacc ggtcacacgg ctctgccgcg cagactccta
     4081 gcctaaatgt gacagtggtt ggtaatccat gccgcccgca agggcggcgg tggtgggcca
     4141 atagatgatt ttcagagcct tactgcccgt tgacgcccaa tccctcgagc gcgagtctag
     4201 ccgcagcggc gatgacggcc tcgctgtgct tgtcatcctt gttaggcgcc cgggtgtaga
     4261 cggccaacac aataggtgcg cgcccagtgg gccagacgac ggcatagtca tttgccgtgc
     4321 catacactcc gcaggttccg gttttgtctc cgactgccca gtctgccggc accgccgcgc
     4381 ggatgcggtg gttgccggtc gtgtttccct ttagccaatc aacaaactgc tgccgctgcg
     4441 gcgcagccag tgcagagccc agtgtcagtt tttgtaagct ttccgtcacg gcgcgcggcg
     4501 atgaggtatc gcgcgcatcg cctgggatgg cggagttcag ctccagctcc cagcggtcca
     4561 gacggaacgt ggtatcgccg atagagcgca tgaaggccgt cagcccggcc gggccgccca
     4621 actccttcag caacaaattg gcggcggcgt tatcactgta ttgcacggcg gccgcggaca
     4681 gctccgccac cgtcatgcct gttgtcagat atttttccga gatgggtgac cacggaacca
     4741 gcgcattttt gccgtaacgg atgggtgtgt ccagcaagcc ggcctgctgc tggctgcgag
     4801 ccagcacagc ggcagcaaga aagcccttga atgagctgca cagtgggaag cgctcctcag
     4861 cgcggtaact tacagttgcg cctgagccgg tatccatcgc gtacacaccg atggagccgc
     4921 caaagtcctg ttcgagttta gcgaatggtt ccgcgacgag gttggtcagc gcggtggcag
     4981 aaaagccagc cagcggccat gagagacaag acagcagaac tagacggcga tacagtgaca
     5041 tcaacgatat tccttgtttg aaggtggagc taggtgtagc ggccggatgt aatcaggccg
     5101 ctacagctgg gattaggggt ctgacgctca gtggaacgaa aactcacgtt aagaagtcat
     5161 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5221 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5281 tgacgtctaa gaaaccatta ttatcatgac attaacctat atgtcaagac ccggctggtt
     5341 atacacgcgt ttcctgaaca attcagggcg tttttcatgc cattccttga gcgcctgaac
     5401 gggtgttttg tgttggagcg cgcgttgcgg aatgctgtgg ttgtagatct tgacgtaatt
     5461 gcgcagcgtc gattccagtt cggcagctga accaaaacgg gtctggttga cgatgtcgct
     5521 gatacgaccg ttgaagcgct ccaccatgcc gttggtctgc ggatgacgag gcgggatgag
     5581 ccggtgctcg atgccgagct gcttgcacag gcggtcgaac acgtgtgtgc cgctgggttc
     5641 cttcttcttg ccgccagccg tgaagcggtc ggtgaactgg ctgccgttgt cggtcagaag
     5701 cttgacgatc ttgacgggac aggcttgctg gactttgttg aggaagtcgc cactgctgcc
     5761 atcggtctgg tcggcataga gctcgatgaa gacccagcgc gtagcacggt cgatggcaac
     5821 gaagagatag cgccttgccg tctcgtcggg catctgcggc aggtacttga tgtcgatgtg
     5881 cacaaagccc ggctcgtagt ccttgaaggt ctttttcgtg gcgggcgcag tgccttcctg
     5941 ctcgaccagg ttacgtagat ccgagacacc gtggcggcgc aggcaacgtc ccaggccggc
     6001 acgcgagacg gctggattga tgaactcgcg cgtgaccgcc agcaggtcgt ccgtgggcag
     6061 cagcaacgtc ttgcgcagct ccaccacgat gagctcctgc tcgggcgtga gcgtcgtgta
     6121 catcttgttc ggcgcatgcg acttgtcttc aggagactcg cggttttgcc actttcggat
     6181 ggtttggcgg gtgacgttgt acatcctggc cagttcggcc tgcgggagcg tcgagttcct
     6241 gatttcctca cggatcaggt gggtagtacg ggcttggctg tgcagtgcct gggtcatcga
     6301 gttgccagtg aagagtcgga ttggaacagt gtacgcaaaa cgtgtctggc cttgaagagc
     6361 ggccagctac gcatgggtac atgatcccac gagtccagac acctataaaa ataggcgtat
     6421 cacgaggccc tttcgtcttc aagaatttta taaaccgtgg agcgggcaat actgagctga
     6481 tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg tcagcacgac gttcctgtcc
     6541 acggtacgcc tgcggccaaa tttgattcct ttcagctttg cttcctgtcg gccctcattc
     6601 gtgcgctcta ggatcctccg gcgttcagct tgtgccacag ccgacaggat ggtgaccacc
     6661 atttgcccca tatcaccgtc ggtactgatc ccgtcgtcaa taaaccgaac cgcgacaccc
     6721 tgagcatcaa actcttttat cagttggatc atgtcggcgg tgtcgcggcc aagacggtcg
     6781 agcttcttca ccagaatgac atcaccttcc tccaccttca tcctcagcaa atccagccct
     6841 tcccgatctg ttgaactgcc ggatgccttg tcggtaaaga ggcactgttg caaagttagc
     6901 gatgaggcag ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg
     6961 cgcatttcgc ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca
     7021 cgcatcacct caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc
     7081 gtggcgccga ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac
     7141 ttaatctgtc ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc
     7201 gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg ccacttcttc
     7261 acgttgttga ggattttacc cagaaaccgg tatgcagctt tgctgttacg acgggaggag
     7321 agataaaaa
//
