LOCUS       NZ_CP109989            10060 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP424 plasmid pKP424-7, complete
            sequence.
ACCESSION   NZ_CP109989
VERSION     NZ_CP109989.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31413558
            Assembly: GCF_025946965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   He,F.
  TITLE     Global emergence of carbapenem-resistant Klebsiella pneumoniae
            co-carrying multiple carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2022) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,643
            CDSs (with protein)               :: 5,643
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 72 of 183
            Pseudo Genes (incomplete)         :: 116 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 34 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109989.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP424"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP424-7"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-10-28"
                     /collected_by="Fang He"
     gene            join(9959..10060,1..264)
                     /locus_tag="OJ961_RS29600"
                     /old_locus_tag="OJ961_29605"
     CDS             join(9959..10060,1..264)
                     /locus_tag="OJ961_RS29600"
                     /old_locus_tag="OJ961_29605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            439..717
                     /locus_tag="OJ961_RS29605"
                     /old_locus_tag="OJ961_29610"
     CDS             439..717
                     /locus_tag="OJ961_RS29605"
                     /old_locus_tag="OJ961_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            705..1019
                     /locus_tag="OJ961_RS29610"
                     /old_locus_tag="OJ961_29615"
     CDS             705..1019
                     /locus_tag="OJ961_RS29610"
                     /old_locus_tag="OJ961_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1183..1611
                     /locus_tag="OJ961_RS29615"
                     /old_locus_tag="OJ961_29620"
     CDS             1183..1611
                     /locus_tag="OJ961_RS29615"
                     /old_locus_tag="OJ961_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1637..1816
                     /locus_tag="OJ961_RS29620"
                     /old_locus_tag="OJ961_29625"
     CDS             1637..1816
                     /locus_tag="OJ961_RS29620"
                     /old_locus_tag="OJ961_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1843..2373)
                     /locus_tag="OJ961_RS29625"
                     /old_locus_tag="OJ961_29630"
     CDS             complement(1843..2373)
                     /locus_tag="OJ961_RS29625"
                     /old_locus_tag="OJ961_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2380..3111)
                     /gene="mobC"
                     /locus_tag="OJ961_RS29630"
                     /old_locus_tag="OJ961_29635"
     CDS             complement(2380..3111)
                     /gene="mobC"
                     /locus_tag="OJ961_RS29630"
                     /old_locus_tag="OJ961_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3111..5075)
                     /locus_tag="OJ961_RS29635"
                     /old_locus_tag="OJ961_29640"
     CDS             complement(3111..5075)
                     /locus_tag="OJ961_RS29635"
                     /old_locus_tag="OJ961_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6559..6708)
                     /locus_tag="OJ961_RS29640"
                     /old_locus_tag="OJ961_29645"
     CDS             complement(6559..6708)
                     /locus_tag="OJ961_RS29640"
                     /old_locus_tag="OJ961_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6793..7050)
                     /locus_tag="OJ961_RS29645"
                     /old_locus_tag="OJ961_29650"
     CDS             complement(6793..7050)
                     /locus_tag="OJ961_RS29645"
                     /old_locus_tag="OJ961_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7060..8745)
                     /locus_tag="OJ961_RS29650"
                     /old_locus_tag="OJ961_29655"
     CDS             complement(7060..8745)
                     /locus_tag="OJ961_RS29650"
                     /old_locus_tag="OJ961_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9072..9317
                     /locus_tag="OJ961_RS29655"
                     /old_locus_tag="OJ961_29660"
     CDS             9072..9317
                     /locus_tag="OJ961_RS29655"
                     /old_locus_tag="OJ961_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9591..9962
                     /gene="tolA"
                     /locus_tag="OJ961_RS29660"
                     /old_locus_tag="OJ961_29665"
     CDS             9591..9962
                     /gene="tolA"
                     /locus_tag="OJ961_RS29660"
                     /old_locus_tag="OJ961_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             2060..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5878..6456
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2384..3111)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat
       61 ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg
      121 gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt
      181 gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc
      241 aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga
      301 tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa
      361 aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat
      421 gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag
      481 gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag
      541 gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag
      601 gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc
      661 tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga
      721 tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc
      781 ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat
      841 tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg
      901 ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg
      961 tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat
     1021 aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg
     1081 tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag
     1141 aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc
     1201 aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta
     1261 aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca
     1321 gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca
     1381 caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa
     1441 ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa
     1501 gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca
     1561 acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc
     1621 gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat
     1681 taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc
     1741 cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa
     1801 gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg
     1861 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
     1921 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     1981 ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     2041 aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca
     2101 cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta
     2161 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     2221 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     2281 caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
     2341 cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     2401 aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg
     2461 tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
     2521 gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc
     2581 ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac
     2641 cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg
     2701 gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac
     2761 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
     2821 cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac
     2881 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
     2941 actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag
     3001 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc
     3061 gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc
     3121 cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc
     3181 agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc
     3241 cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg
     3301 tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     3361 ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg
     3421 tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg
     3481 cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc
     3541 agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag
     3601 tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc
     3661 tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt
     3721 tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc gtccatcgag
     3781 ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacatcgat accgctgtcc
     3841 gtctgcagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc cttcacgtcc
     3901 tttttcaggg cctccggcaa cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
     3961 tgcgggaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
     4021 gccacgtcgc cggtctcact caggttaaac ccggcaacca gcagattctt cagggcgtac
     4081 tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg
     4141 gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc tttggggtcg
     4201 aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc
     4261 gcgctcttgc cgctcccggg caggccgagg atctgaatat tggtcttgcg ccaggtatca
     4321 cgagggacca gcaccggctg gccctgctca tccttaccca gaaacaccgc ctgctgtttc
     4381 cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc cccgaaatgc
     4441 aggtggcgga cgtctgtcat ttctccggtc cgctggctgc tgcgccggct ctcctgattc
     4501 agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac catcaagcag caggaacgcc
     4561 agcagccgcg ccagaaggac gccggcgaca agccctccgg cacaccaggc tatcccgaac
     4621 aggttagccg gctgcagcag gtaataatct gcgttcgcat agaggtccgg gcgcaggacg
     4681 gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg
     4741 gcagcgccgg tcagccagta cgcgtagttc cagacatgtc gccgtgccct gaccatccgc
     4801 ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag tccggttatt atcgcgctta
     4861 tagcgatcgg catactcccg ccgcgcctta tggctcagcc ccacaaaacg cattcggccg
     4921 gcccagagtt cagccagacc gaaaaacagg ccgataacca gaaaaaaacc ggccagactg
     4981 taatactccc ggtacagcca gctgacccac gggtaaatct gccgggccag ctccgggata
     5041 tgccactgcc aggcaagcga atccgtatta aacatgcaat ctctctcctt cagacgacgg
     5101 gtttaccggc gtgatgcgca gcccccgctg cacaagccgc ggacatgccc caaggcatgg
     5161 cggcggcggt cacgtccgtg gtgattgcgt tcagctttca ccgcggaggc cagcctcttt
     5221 ccgccccatg cggaaaaggc gcagccccgg aggaacaacg agcacgcccc agcgtgtgag
     5281 aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc cggcccccgt cagagttcgc
     5341 catagcgagc ggctgaccac ccgcaaaatc aggagcagct ttgctgcttc gtcaggtgcg
     5401 tccagcaccg tggatttttg tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt
     5461 tctggcgtgg ccggcgtcca gccggacaga gccagaaaga ggggaaaaga ccgtgcgtcg
     5521 gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa gtcagccacg ccccagcggg
     5581 gatgacttgc atttctccgc aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt
     5641 tcagcgaaaa accgacatgg ttcacctatc ctgataattg atcgtcaggg aatagaaaga
     5701 cttaatcagg ggacaatatc ccacatcagc gcgtccggtg accgtcttgc aggtgccggg
     5761 gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg
     5821 ctgcggacac atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga
     5881 ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag
     5941 ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat
     6001 ctgacagtac gggcgaaacc cgacaggact taaagatccc caccgtttcc ggtaggtcgc
     6061 tccctcttgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgccgttctc
     6121 ccttgcggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag
     6181 gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc
     6241 ttatccggta actgttcact tgagtccaac ccgggaaagc acgcaaaacg ccactggcag
     6301 cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt
     6361 gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga
     6421 aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc
     6481 cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag
     6541 aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg
     6601 tgccaccgtt ccgccctgaa catcccggat ataatttgcc tgacatgcgg ccagaagaaa
     6661 gccagcaaca atcaatataa aaagaaaaat agtctttacc tttttcatat actttccctg
     6721 tatttaaaga ctccggcatt catccctgaa caccggaagt cagtatttca aatcatcctt
     6781 gataattttt tgttaccagt taccgtcccg ataatcaaaa gctatataac aattatccac
     6841 atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa ggaacccatt ctttcttcaa
     6901 atcaaactca ccattattaa tattatcctt taaaggcatt cccaagcttt caatgacaga
     6961 accatcgtca ccaaagtctt tagaatactc ttccccttta aactcttcag ttttcttatc
     7021 aaaccatgtc aaacgtaatt taagccccat atttacctct caaagatatt ttttaatgtt
     7081 ccgttttgga tctggccctt taacctgctt acccgttttg ggatcaaatg cgcctaggtg
     7141 ttcgccatca ctggcacgat acccttcaag ctcaccgtgc tgggaatccc attcataaat
     7201 tttacgtttt ttatcgccat accatcgggc ccgcttacca ccaccacctt gtttaggtgt
     7261 tttaggatca cctcgcttta actctccaag tccctttatg tcttctgttt taggtactgg
     7321 atgataatca tggccgtagt ctttagtccc cttgcgaggc ttgttctttt cctcatttaa
     7381 cttattttct gcatccttcg cttttttctc cttttgtttc cggctctcca tcgccccggt
     7441 aagtgcagcc ttatctctgg ataaggattc attcattgcg tttaaagatg cctgagccgc
     7501 ttttaatttc tcattctgat tctgaatatc cttatcaaga tagcgaacct gatcctgcca
     7561 cccggagcca tattcatgag gcgggttata agtcacaaaa tcatctttat ttgcctctgc
     7621 ctcttttacc tttttatcgg cttcctgaac agcagccctt gcaccgggga gcgccttctc
     7681 tgtcgatgca atgcggctgt ttaaggtcgc aatattttta tcttcggcat ccagctcagc
     7741 tttcgcttta tcatagtctc ttttcagccc ctcttccgga tgagcggcgt cccatgcctg
     7801 ctggcgacgc ttttcttcct cctgacgctg ttttacctgt gccggggtaa gaacatctgt
     7861 caccgacaca taaaccggct tctgtccggt ctctttcggg aaacgaataa cggcctcacg
     7921 ggagttacca ccggctgtaa aaccagccgg acgtgaatca cctttttcag caacaatgcc
     7981 ttttggcggg gctttcgctg tcggaacacc tttcggtacg ctcacctgca gagacgggag
     8041 acccggaatc gacacactga atacccccgg acgttttgtc ggtttcgcat ccaccacagg
     8101 gacactcatt ggccgacctg cgacaaccgc aatatgctgc cgctcatcct tcaccacatc
     8161 cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt ggtaaagtac ttgccggagt
     8221 ctccgtcacc gcatcggccg gtaatgacgt cataattttt gacatcattt tcggatcatc
     8281 ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc ccccacagac caaacttaaa
     8341 cggccccttc agggcagcca gcacatcagc aacggcggcc gacaacgcat cgccggaaac
     8401 tgataaagcc agtccttcag cccccggagt agccagggca ggcagaccga aggccatcgc
     8461 agtggccgaa gactgtccgc caccagagct accgctatga ttgccgctgt taccaccatt
     8521 accaccgggt ttactcccgc cgttaccaaa ctcccccgga ttatagttat gaatatctcc
     8581 attcggcgta tgcgtcgtac cccagcccgg cccgctgttt ggatccgtac caccacctga
     8641 tgaagggcca cctttaccag acgtaccgtt cacattccca ctggcctgac caccattatg
     8701 tcccagacct gaattacccg gacctcgacc gtctccacca ctcataccgt tttcctcttt
     8761 gacatttaaa acaataaatt aaagccaaca tactgtacat atacacagta taattattac
     8821 cttactcctc ccgttttcag gtgtcaatat atgagattag ttctcatctt caggcacaaa
     8881 aaagcgcccg gagccacctg taagcagatc gcgtcttttc ccgaaaattt ctcatggcaa
     8941 caaattttat tatttaagac attcatcaca cttacatttc agatggtata gacacatctg
     9001 aaagctaact ggcatgagga gaggacctgc ggttaaactg ttcgggaaac ctgagacgcc
     9061 cggaggccac tatgacgcac ctgaaactga ctcccgctga agaaaagcgc ctggcggacg
     9121 cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa caaaacgctg ccaaaggcgg
     9181 taaaacaaca ggtgcgggaa agggtgcttc gtgccattca ggacgaacgt gaagccaggc
     9241 gggcgcagga aatgcgtcgg gagaaatacg aggctacgga aaccaccttc acctggcagc
     9301 cacagcgccg ccggtaaggg gtatttacca taacgtcgca ttgcccgcac cgctcagacg
     9361 gtctgagggc gtttccggcg gctacgggat aaaacaggca acaaaccacc cggaaaaccg
     9421 ccggaaacgc gcccggtaaa ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg
     9481 ggccgtttgg tgtaaaaacg atacgataag ccgtgtttat gtaaatgaac gtggccgtaa
     9541 agagtgacaa atatacatgt aacatttttt actgacaggt catcaacccg atgcgtttaa
     9601 taccctttat cctgctggca ttgtgcagcc ccttactcct gacggcctgt aagcattcca
     9661 ccacccagcc gccgaaaatt tatactctgg ataagtcatc taaaatcagt agctacgctg
     9721 cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa
     9781 tgtgcaccgt gcggatatcc atacaacgtg atggcactct gaacagcgcc attgctgaag
     9841 gaggagatcc gaaactctgt aaagcagcaa tatcagccgt tacccacgcc aaaattccgc
     9901 cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc ccctctggat ttcagaccat
     9961 gaaagctttt actctgacag tgctggcggc catgttcctc actccggtac atgcgatacc
    10021 actaaaaccc gtaaccacac tgcagccaac ctacccggta
//
