LOCUS       NZ_CP109988            11057 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP424 plasmid pKP424-6, complete
            sequence.
ACCESSION   NZ_CP109988
VERSION     NZ_CP109988.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31413558
            Assembly: GCF_025946965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   He,F.
  TITLE     Global emergence of carbapenem-resistant Klebsiella pneumoniae
            co-carrying multiple carbapenemases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2022) Department of Clinical Laboratory, Zhejiang
            Provincial People's Hospital, 158 Shangtang Road, Hangzhou, Zhejiang
            310014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,643
            CDSs (with protein)               :: 5,643
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 72 of 183
            Pseudo Genes (incomplete)         :: 116 of 183
            Pseudo Genes (internal stop)      :: 34 of 183
            Pseudo Genes (multiple problems)  :: 34 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109988.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP424"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP424-6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-10-28"
                     /collected_by="Fang He"
     gene            107..804
                     /locus_tag="OJ961_RS29545"
                     /old_locus_tag="OJ961_29550"
     CDS             join(107..356,356..804)
                     /locus_tag="OJ961_RS29545"
                     /old_locus_tag="OJ961_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225739689.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLQSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVNSKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEQHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            1269..1379
                     /locus_tag="OJ961_RS29550"
                     /old_locus_tag="OJ961_29555"
     CDS             1269..1379
                     /locus_tag="OJ961_RS29550"
                     /old_locus_tag="OJ961_29555"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142740574.1"
                     /translation="MDWILLAIAIALLVVAIYSGISYFREKKSISRVFRR"
                     /name="small membrane protein"
     gene            2428..2649
                     /locus_tag="OJ961_RS29555"
                     /old_locus_tag="OJ961_29560"
     CDS             2428..2649
                     /locus_tag="OJ961_RS29555"
                     /old_locus_tag="OJ961_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            2642..3529
                     /locus_tag="OJ961_RS29560"
                     /old_locus_tag="OJ961_29565"
     CDS             2642..3529
                     /locus_tag="OJ961_RS29560"
                     /old_locus_tag="OJ961_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_114450885.1"
                     /translation="MAKFASAARQATGVIKILQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            3526..4224
                     /locus_tag="OJ961_RS29565"
                     /old_locus_tag="OJ961_29570"
     CDS             3526..4224
                     /locus_tag="OJ961_RS29565"
                     /old_locus_tag="OJ961_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101975735.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATAIAATAFSLWLFILFFRSSESRRIQSETDRYKQALREAEEAHTARQNALI
                     RDHNMKTETLHSHYCDAANVLREEYAKREKRWRQALQNAREEARQELEEQTEALNQRAS
                     GLLVQEQTLKARMEEVRKYVAGERHNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(4231..4635)
                     /locus_tag="OJ961_RS29570"
                     /old_locus_tag="OJ961_29575"
     CDS             complement(4231..4635)
                     /locus_tag="OJ961_RS29570"
                     /old_locus_tag="OJ961_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_114450884.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVSVEQEEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4635..4826)
                     /locus_tag="OJ961_RS29575"
                     /old_locus_tag="OJ961_29580"
     CDS             complement(4635..4826)
                     /locus_tag="OJ961_RS29575"
                     /old_locus_tag="OJ961_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5246..5359
                     /locus_tag="OJ961_RS29815"
     CDS             5246..5359
                     /locus_tag="OJ961_RS29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073511950.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHANAPLAAATGN"
                     /name="replication initiation protein"
     gene            5810..>6489
                     /locus_tag="OJ961_RS29580"
                     /old_locus_tag="OJ961_29585"
                     /pseudo=""
     CDS             5810..>6489
                     /locus_tag="OJ961_RS29580"
                     /old_locus_tag="OJ961_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            <6598..7565
                     /locus_tag="OJ961_RS29585"
                     /old_locus_tag="OJ961_29590"
                     /pseudo=""
     CDS             <6598..7565
                     /locus_tag="OJ961_RS29585"
                     /old_locus_tag="OJ961_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(7974..8852)
                     /locus_tag="OJ961_RS29590"
                     /old_locus_tag="OJ961_29595"
     CDS             complement(7974..8852)
                     /locus_tag="OJ961_RS29590"
                     /old_locus_tag="OJ961_29595"
                     /inference="COORDINATES: protein motif:HMM:NF025663.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="WP_157280122.1"
                     /translation="MIKFFFGKAYRLLLENLSTTNRVKLRYFRVYKKLPNIKKPKTFNE
                     KVISRILGEQKDIYSTLADKYLVRRFVSERVGEEYLIPLHLATSNPSDLLKIDDWSGLV
                     IKPNHAAGMIYISDDNPSKEEKDNVVTTAKTWLKTDFSSFCDEMHYSKIKPMLLVERKI
                     TKRGEIPRDYKFHCFKQKNGDINFVLQLVDGRFGQESRSYFLNSFENMVWSHGAGNHLL
                     SNNEVAVLNEVILLNKRLILNDFNYVRIDWYILGDRIYFGEITFTPGAGIGNEFGPVLE
                     KQMAEWWEDEY"
                     /name="ATP-grasp fold amidoligase family protein"
     gene            complement(9133..10356)
                     /locus_tag="OJ961_RS29595"
                     /old_locus_tag="OJ961_29600"
     CDS             complement(9133..10356)
                     /locus_tag="OJ961_RS29595"
                     /old_locus_tag="OJ961_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_264526591.1"
                     /translation="MMAEKLVSIIGLVFINSYMAKYIGPTDFGKITFATTIFTFVQTMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDVFAVYNNTQLISRINALTNGAGLCIAFIVRFLISYLELPVYTFSIPII
                     LIPAIPYVIRYIYFHNTIEDTAVLKSKTKKYNKYLFSAGGSLLLSTLSIVIYTQVSNIF
                     LAKITSYSDLGIYNVALTLGSAYSFIPVALITSFFSKIYQEKDFEKMSRLLTLINRIVI
                     VITIFILFGFYFLGPCIISMLYGSAYKLAVELVPIIILATMFSSLGTICYRFIIKYSGY
                     KYLSYKMTLTSLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRNGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     Typing          4875..5359
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.984"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 ctgaatcagg ttgaaaacct gatcacagag cctatggaac acataattta aggtaatgct
       61 gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc
      121 catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc
      181 cggacatcag cgctatctct gctctcattg ccgtaaaaca tggcaactac agttcactta
      241 caccgcctct cagcccggta cgcaccagaa aatcattgat atggccatga atggcgtcgg
      301 atgtcgcgcc agtgcacgca ttatgggcgt tggcctcaac acggttttac gtcacttaaa
      361 aaactcaggc cgcagtcggt aacctcgcgc atacaaccgg gcagtgatgt gattgtctgc
      421 gctgaaatgg acgaacagtg gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt
      481 tacgcgtatg acaggatacg gaggacggtt gtggcgcacg tcttcggtga acgcactctg
      541 gccacactgg agcgtcttca gagcctgctg tcggcctttg aggtcgtggt atggatgacg
      601 gatggctggc cgctgtatga atcacgcctg aagggaaagc tgcacgttaa cagcaagcgt
      661 tacactcagc gcattgagcg acataacctg aatctgagac aacatctggc aaggctggga
      721 cggaagtcac tgtcgttctc aaaatcggtg gagcagcatg acaaggtcat cgggcattat
      781 ctgaacataa aacactatca gtaagttgga gtcattaccc acgtctcgat atcaccaccc
      841 acagccttta cgcctggata aagaagtacg gtccggatac cggatcccgc gggcacgtaa
      901 aggcgaggcc agtatcgtgt cgcccaacag gctccagcga cagttcaatc cggatgctcc
      961 ggatgagcgt tgggtaacgc cccaataggc agtgtaagtt aactgtcgct gccgcgtttt
     1021 acggcagtgg ttgcagacaa gcaggggggg gggaacatgc ccataactca tgcacggttt
     1081 ttttgttttt cctgtatgta caaaaaacat aactaggaat aatcttagca gccttaacaa
     1141 acacatacaa aaacagtggc tatgtttcat ctcaccacaa taagtaacga cagtatcatg
     1201 ctaaaaaaag atgatattat ctttatgaaa tcataacagg ctgtttttat taaattaatt
     1261 aggcatttat ggactggatt ttacttgcta tcgccattgc tctacttgtt gtagctatct
     1321 actcaggaat ctcatacttc agagaaaaaa aatcaatcag tcgtgtattt aggcgatagc
     1381 cccctattta ccataatccc ttcttacgcg caccgcgaaa acggcctcag ggcgtttctg
     1441 gcaggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcacctgat
     1501 ttattttacg catatgcatg gctatgcaca ccaccgcata gccggtgatt tcggtcgaga
     1561 aaagggttct ggacgttaaa agcataaaag aacgggccat ccaggcccgt caaatgagga
     1621 caacactgta aaaggaccgt cgcacctggt gcggtgccgg cgataagaaa aaggtagctc
     1681 atatcagccc ctcaccggag aaaaatattt cctggccacc tcaaccgcca cacgctgtgt
     1741 tcccgttaac tcgcgcagat aacgcaggaa catcctgttt tcctctgcat gcgcctcctc
     1801 actgccctgt gcgtggtatt ccactatcgc gttcaccacg gactcaccga cctgcatcgt
     1861 ctgacctcat ctgcactttc atcacaccgt ttcagatccc gcctcctcgc catgatgtga
     1921 aacggtgacc gggcaggcaa attttttgtc tgtccggtca ccgttggcgg ggtccggggc
     1981 ggtcagtccc ggtgtttttt attccgccaa atcagcacct gcgacacccg ctatgtacga
     2041 cgcataaatg cgcctgtaca tagcgggtgt cgctattgag cggagtcaga cgcaacattt
     2101 aaaaaacaac attcacacgc acgaaaaacc tggctagtat ttagtcaaac catgagcccg
     2161 cgcactctga cgcgcacctg cctaccggca gcaggtaaaa cagccgggtc cctccgggga
     2221 tactgtaccg ggcagcccaa tccgagaaca ctgagacaac agcccaaatc tgtcagaccc
     2281 acaaccgggt caagtcattc tctgactaaa gagacgttga gccgcgagca gtccgggctt
     2341 aaccacgtta cggtttaaac gagggcgatc ggcgcaggcc gcagtggatt tccactggcg
     2401 ttcttacaac tcaacaccgg agttcctgtg gaatttgata ccgccgccga actggccgcc
     2461 ctgcaggcgc agacccgccg tatccgccag gtccgctacc gtccctcccg ccttgaccgc
     2521 tataccggcg aactgctgag cctgtaccag gccggggcca gtgcagcgga gctgcaacgc
     2581 tggctccggg cccggagaat caaagtggtg ttgtccacgg ttacccgctg gctggaaaaa
     2641 aatggctaaa ttcgccagcg ctgcccgcca ggcgacgggc gtgataaaaa tcctccaggg
     2701 cgatattctt cgctctgtcg gtacggtccg gaattacgag cagggactga cccgcgtcgc
     2761 agagtggatg aaagataacc gcatcaacca cggtgattta cgcacactga caccggcgca
     2821 ggccatcacc tacctggagc agcgcgggga gcaggtcggc cagaaaacgc tcgatatgga
     2881 acgccaggcc atccaggcca tgatgatcca cgtgacgcag accctccctg ccggcgaaca
     2941 cctgccggtg gtgaaatcgg agcaggcaca gatcctgagc agccgggcct acagcccgga
     3001 ccaggtggcc ctgattgccg ctgcacagca gcccgccaac gctctggcca ccgcgattgc
     3061 tctggccgcc ggattacgcg cccatgaact gctgaccctc gcgccccgcc aggagcgagc
     3121 agcggatccc cgtccggcag cggacagtaa atttgccggt cgggagggtg tgatttacac
     3181 cgttcagggc aaaggcggcc tggtcaggga agttctgctg ccgtcacggc tggctgagcg
     3241 tctggagcat gtcaggctgg cgagcccggt ccgggtgacc gatcgcggcg tgttttacca
     3301 gagtcagtac gccattggcg gaggacagcg ctggtcgaac gccttcagtg ccgcctccat
     3361 gcgtaccctg ggctggtcac ggggggcaca cggcgtgcgt cacagctacg cccagcaacg
     3421 gatgcaggag ctgcaaaaac tcggtctggt tcgcgatatg gccctgcgca ccgtcagcca
     3481 ggaaatgggc cattttcgtc cggaaatcac ggagacatac ctgcgatgac aagaacgttt
     3541 attttattgt gtaccggtgt tttcgccatc gcgtacgccc tgtatgagct gaaacatatg
     3601 ggcgtttact atccggatgc cagcaatccg cagctgttca ctggctggaa ggcggtcacc
     3661 gaactcggtc ccgctatcct ggctaccgcg atcgcagcca cagcgttctc gctgtggctg
     3721 ttcattttgt ttttcagaag ctcagagtcc cggcggatac agagcgagac agacaggtat
     3781 aagcaggctc tgagggaggc agaagaggcg cataccgcca gacaaaatgc ccttatcagg
     3841 gatcacaaca tgaagacaga aaccctgcac agtcactatt gtgatgcggc gaatgtactg
     3901 cgggaagagt atgcgaaaag agaaaagcgc tggcgtcagg cgttgcagaa tgcccgggaa
     3961 gaagccaggc aggagctgga ggaacagacg gaggcactga atcagcgcgc gtccggcctg
     4021 ctggtacagg agcagacact gaaagccaga atggaggaag tcagaaagta tgttgccgga
     4081 gagcggcata acgccatgcg ggcccgtctg gaggcagagg aagcagagaa aaggcgacaa
     4141 aatgccagcg caacagcgga acggcgcaga cggaaactgg aaaaactcca ggccactgac
     4201 ggaacagcag ggcaaaaaca ctgaggcatt tcaggactgc cagaaacgcc accaggcacg
     4261 gggccgggtc gtgttctgcc gcttctcttc ccggttctcc agcaacaaca tcgcctgttt
     4321 cagactgtcg atatgtgaat ctctggaggc aaggagggtg ttcaggtgtt caatttcctg
     4381 tttcagaatg taaataacct gttcattacg gtcagaagtg actgtgtcac gctgtccact
     4441 gacctgttct tcctcctgtt caacggatac gggcgcaaag gcaccaaaca cccgcaaaag
     4501 ctctgaagta tcgagcattt tcgcattccg ttcaccccga caggtggaca gttcaccggc
     4561 tttaattaac ctgtgcaggg ttgtcctgct cttacccgtc aggcgtgcag cctcactgat
     4621 cgacactttc gccatcagtc accctcctgt agtgcatcca gtctgaggga cagtgtacgg
     4681 aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt cagcctccgc atcgagatca
     4741 agctcgttaa gcttctccag caggagcagg gactgattct ggatgaattt agccatgttc
     4801 agggcagttt tctgttgttt cgtcataagg ggcctcaggg cgtcagtgct cagtgtaaaa
     4861 cctgtctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg
     4921 cgagcggacg gggcacacag acagaaaagt catcgccacc acccgggaaa ggaaaagaac
     4981 agacgggaga aggccacggc gttgccgttt ttccataggc tccgcccccc tgacaagcat
     5041 cacagaaacg gacgctcaaa tcagcggtgg cgaaacccga caggactata aagatcccag
     5101 gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     5161 gtcattccgc tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggaa
     5221 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc
     5281 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaatg cgccactggc
     5341 agcagccact ggtaactgat ttagaggaga tagtcttgaa gttatgcgcc agttaaggct
     5401 aaactaaacg aaaagttttg gtgactgcgc tcctctaact agccagttac ctcggttcaa
     5461 agagcgccaa ctcttggaac cttcaaaaaa accgcccgcc aaggcggttt tttcgtttac
     5521 agagcaagag attacgatgc caaagaaagg atctcaagag gatcctgttc catatacctt
     5581 cccctgaagg gaaaccagcg acgccagtga actaacagaa aacaaaagca tttgatctta
     5641 ttaaaaaaaa tgaatataga gtaagatttt acacaatcag tggctttgtt gaataaatcg
     5701 aaattttggt aacctgaaga atcagatcac gcattatcct gactatgcag gcagttccgt
     5761 ggcaaagcag aaattcaaaa tcaccaactg acgcaactac aacaaggccc tgattaatcg
     5821 tgactccatc actttctggg ttgatgatga ggcgactctg gcctggtatg agtcagcaac
     5881 gcctgcatcg cggggacaac ctcagcgcta tacagcccct gccatcacaa ccttcctggt
     5941 gattaaacgt gtattccggc tgaccctgcg aaccgctcag ggctttattg attccatttt
     6001 tgccctgatg gatgtcccgc tgcgctgtcc ggattacacc agtgtcagca agcgggcaaa
     6061 gtcggttaac gtcagtttca aacgcccacc cggggtgaaa tttcgcatct ggtgattgat
     6121 tccacagggc tgaaagcctt cggtgaaggt gagtggaaag tcaaaaagca cggcaaagag
     6181 cgccgtcgta tctggcgaaa actgcatctg gctgttgacg cgagcaggca tgaagccatc
     6241 tgctcggacc tgtcactgaa caacgtcacg gacgctgaag ctttcccggg gcttatccgg
     6301 cagacccacc gaaaaatcag gtcagcggcg gcggacggcg cttacgatac gcagcgatgt
     6361 catgacgaac tgcgtcgcaa gaaaatcagc gcgctcattc ctccccgaaa gggagcgggc
     6421 tactggccgg gtgtgggaac agccctgcga aaccttgcag cagcgttcac gcattacaat
     6481 gaaaatcacc cgcccagcgc gctgggatat cactctccga gagaataccg gcagctgcgg
     6541 gcatcgttaa cttaagatac aaaagctgtc cggagatggc gggtcaagat cactcaggcc
     6601 gagatccgcc gtctccagaa agagctgaaa cgggttaccg atgaacggga catattaaaa
     6661 aaagccgcgg cgtacttcgc aaaactgtcc gactgaggta cgcctttatc cgtgacaact
     6721 cctgttgctg gcctgttcgc ctgctctgtc gggtgctgga tgttcatccc agtggttttt
     6781 acgcctggct tcagcagccg cattcacaac gccatcaggc agacctgaga ctgacaggac
     6841 agattaaaca gttctggctg gaatcgggat gcgtctatgg ttatcgcaaa atccatctgg
     6901 atctgcgtga cagcgggcaa cagtgcggaa taaacagagt ctggagactg atgaaacgtg
     6961 tcggaataaa ggctcaggtc ggataccgaa gcccgcgggc acgtaaaggc gaggccagta
     7021 tcgtgtcacc caacaggctc cagcgacagt tcaatccgga tgctcctgat gagcgttggg
     7081 taacggacat aacctacatc aggacccacg aaggctggct gtatcttgcc gtggttgttg
     7141 atctgttctc acgcaaaatt atcggctggt ccatgcaatc ccggatgaca aaggacattg
     7201 tcctgaacgc actgctgatg gctgtatggc ggcgtaatcc cgaaaaacag gtgctggttc
     7261 attcggatca gggcagtcag tacacaagcc atgagtggca gtcgttcctg aaatcacacg
     7321 gcctggaggg tagcatgagc cgtcgcggta actgccatga taatgcggtt gcagaaagtt
     7381 ttttccagtt gttgaaacgt gaacggataa agaaaaagat ctacggaacg cgagaagaag
     7441 cccgcagtga tatttttgat tacatcgaaa tgttttataa cagtaagcgt cggcatggtt
     7501 ctagcgaaca gatgtcaccg acagaatatg aaaaacagta ttatcaacgg ctcggaagtg
     7561 tctagattat ctgtggcgat tcagtccaac ccttacggac acgatacatc gccactggta
     7621 acgggtgtac aagatttact gactcagagt tcttgaagtt aaagccttac tgcggctaaa
     7681 ctgtaaggac aaattaggtt gcagcggtct tgtacaccct gttaccacgg ttcagaagtt
     7741 ccccaactta ctgacccatc gaaaaaacca ccaccccagg tggttttttc gtttctgcta
     7801 cttagattac gatgcagtga ctccaatctc aaggaaaaac gatcaggttt cgaaagtagg
     7861 taagaacatc atcaaaagat caccttgctt gtaaaaagta ccttcagcca aaggcgataa
     7921 tcaagaagaa atagcgcata caacttgcaa ataccacgag cgtagtgcat gaattaatat
     7981 tcatcctccc accactcagc catttgtttt tccaagacag gtccaaattc atttccaatt
     8041 ccagcccctg gagtaaatgt aatctcacca aaatatattc ggtcacctaa aatataccaa
     8101 tcaatacgaa cgtaattaaa atcattaagg ataagtcttt tatttaataa aatcacttca
     8161 tttaaaacag caacctcatt attacttaat aaatgattgc cagcgccatg actccaaacc
     8221 atattttcaa aagaatttaa aaaatagctt cgtgactcct gcccaaagcg accgtccact
     8281 aactgcaaca caaaattaat atcaccattt ttttgtttga aacaatgaaa cttgtaatca
     8341 cgagggattt caccacgttt tgttattttc ctctccacta aaagcattgg ctttatcttg
     8401 gagtaatgca tttcatcgca aaaagaggaa aagtcagttt tcaaccaggt tttcgcagtg
     8461 gtaacaacat tgtctttctc ctctttagag ggattgtcat cggatatata aatcatgcct
     8521 gccgcatgat ttggtttgat aaccaatcca gaccaatcat ctatttttaa taagtcacta
     8581 gggttgctag tagccaaatg gagaggaata agatactcct ctccaacccg ctcggatacg
     8641 aaacgccgca caagatattt atcagccaat gtggagtata tatccttttg ttctcctagt
     8701 attctactaa taaccttttc attaaaggtt ttaggttttt ttatatttgg aagcttttta
     8761 tacactctaa aatatcttag tttaactcta ttggttgtag acaaattctc caaaagcaac
     8821 ctgtaagcct taccaaaaaa aaacttaatc acttcaattc tctcttttta atctgttaaa
     8881 tcagaaagca tgtatactcg agacactcca agtttcatgc gccttggctt cttgatgcac
     8941 tgcagataag ggttccacgg aactcagtct acacactcaa tcttattaaa catacagata
     9001 aataataaaa aatcataagt aacaataaaa ttcaaaaact cacccccaac acaccagaac
     9061 taatcatcat attttaaatc tcataagaaa atcaataaaa cacaatccat aaataaacct
     9121 gcccccccac catcaacgcc tcatcaaccc aataacattt aaatgaatcc ttagaatcat
     9181 tccattccta aagaaataat ttaataatgt gcatgacaga acttcgacca caagaaaaca
     9241 atacgcagca ccgaaaacat cataacggtt aatcataaca tatgagaatg ggatgctgag
     9301 caagctagtt aacgtcattt tatatgaaag gtatttgtag ccagagtatt ttattataaa
     9361 tctataacaa atagttccca aggatgaaaa cattgtcgct agaataatga ttggcaccaa
     9421 ctcaacagcc agtttatagg ccgaaccata gagcatgctg ataatgcacg gcccaagaaa
     9481 ataaaaacca aataatataa aaattgttat tacaatcaca attctattta ttaaagttag
     9541 aagcctagac attttttcaa aatctttctc ttgatagatt ttagaaaaaa aactagtaat
     9601 taacgcaact ggaataaatg aatacgcact gccaagcgtc agcgccacat tataaatacc
     9661 caaatcagaa tatgaggtta ttttagccaa aaaaatattt gatacctgtg tgtaaatcac
     9721 aatggacagg gttgatagta acaacgaccc tcccgctgaa aacaaatact tattatattt
     9781 cttcgttttc gattttaata ccgcagtgtc ttcaatggta ttgtgaaagt atatatatct
     9841 aataacataa ggtattgcag ggattaatat aataggaatg ctaaatgtat atactggcaa
     9901 ctccaagtat gaaattaaaa agcggactat aaaagcaata cacaaccctg caccatttgt
     9961 taatgcatta attctagata ttaactgcgt attattatat acagcgaaaa catccatcac
    10021 tatataataa gtggcaatgc agttagccac cccaaaaaca aatgtcaggg tatctgtaaa
    10081 aaaaaacaaa tagaacaagg caatcaatga agagagtgca aaaataaccc tgcgctgctt
    10141 ttgagtatgt aatgcaagag caatacctga ttttcttttc tcactcatcc ttttaaaaag
    10201 tatgttttgc ccaccaaacc aagccatggt ctgtacaaac gtaaatattg tcgtagcaaa
    10261 agtaattttg ccaaaatcag taggtcctat atattttgcc atataggagt ttataaacac
    10321 aagaccgatg atgctgacta atttttcagc catcatccaa aaggcattag aatacattac
    10381 tcactcgcaa aaaattaatc ccatagaaat tttggtaatg ctgtcaactt gttgataact
    10441 attttaatct gtagtcattc aatccttgta acatcaggat aatcctagtg tcagtgaaaa
    10501 tacgaaatca acaagttggt atcattacca gcgtcggcat ggttccagcg atcagatgtc
    10561 accgacagaa tatgaaaacc agtattatca acggctcgga agtgtctaga ttatccgtgg
    10621 cgattcaaaa gctgattttt gtcgtggtct aactactgca aagaatccac ctaaaagctc
    10681 tcttttgaca tatcttctgc atcttataaa ttgaataaaa cctataaaaa acaaccattt
    10741 aggcacgcta ccgccctagg ctcttcagct acctatccac tgcactaacg ctaacgacta
    10801 tctttaacat ttgggttgag gtcaaatcat taagtaactt aacaataaac acgataactt
    10861 caggaaggac cgatttcacc cttgcttatg atggcgtaac gttttcttat catggaactt
    10921 tcacgagact tgccattttc tgcaaattcc gattccttta cacttatcag tatactgact
    10981 cagtggacta acatcgccgt tgatgcaatt acagaattaa caaaaaagtg gtggccaccg
    11041 ctgcgaatgt acatgac
//
