LOCUS       NZ_CP109978             5596 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Klebsiella pneumoniae strain 857 plasmid pE, complete sequence.
ACCESSION   NZ_CP109978
VERSION     NZ_CP109978.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31396910
            Assembly: GCF_025946985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Lv,F.
  TITLE     Klebsiella pneumoniae Strain 857, complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Lv,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2022) Microbiology Laboratory, Dongguan Tungwah
            Hospital, No. 1, Kefa 7th Road, Dong Guan, Guang Dong 523413, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:36:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,923
            CDSs (total)                      :: 5,802
            Genes (coding)                    :: 5,610
            CDSs (with protein)               :: 5,610
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 65 of 192
            Pseudo Genes (incomplete)         :: 136 of 192
            Pseudo Genes (internal stop)      :: 23 of 192
            Pseudo Genes (multiple problems)  :: 32 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109978.1.
            Bacteria and source DNA available from Homo sapiens.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="857"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pE"
                     /geo_loc_name="China:Dongguan"
                     /collection_date="2022-08-01"
     gene            35..385
                     /locus_tag="OI501_RS29480"
                     /old_locus_tag="OI501_29485"
     CDS             35..385
                     /locus_tag="OI501_RS29480"
                     /old_locus_tag="OI501_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(455..721)
                     /locus_tag="OI501_RS29485"
                     /old_locus_tag="OI501_29490"
     CDS             complement(455..721)
                     /locus_tag="OI501_RS29485"
                     /old_locus_tag="OI501_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(705..929)
                     /locus_tag="OI501_RS29490"
                     /old_locus_tag="OI501_29495"
     CDS             complement(705..929)
                     /locus_tag="OI501_RS29490"
                     /old_locus_tag="OI501_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1596..1808)
                     /locus_tag="OI501_RS29495"
                     /old_locus_tag="OI501_29500"
     CDS             complement(1596..1808)
                     /locus_tag="OI501_RS29495"
                     /old_locus_tag="OI501_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1815..2324)
                     /locus_tag="OI501_RS29500"
                     /old_locus_tag="OI501_29505"
     CDS             complement(1815..2324)
                     /locus_tag="OI501_RS29500"
                     /old_locus_tag="OI501_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2359..3063)
                     /locus_tag="OI501_RS29640"
                     /pseudo=""
     CDS             complement(<2359..3063)
                     /locus_tag="OI501_RS29640"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3002..3346)
                     /locus_tag="OI501_RS29510"
                     /old_locus_tag="OI501_29515"
     CDS             complement(3002..3346)
                     /locus_tag="OI501_RS29510"
                     /old_locus_tag="OI501_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3336..3512)
                     /locus_tag="OI501_RS29515"
                     /old_locus_tag="OI501_29520"
     CDS             complement(3336..3512)
                     /locus_tag="OI501_RS29515"
                     /old_locus_tag="OI501_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4152..4310
                     /locus_tag="OI501_RS29645"
     CDS             4152..4310
                     /locus_tag="OI501_RS29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4668..4994
                     /locus_tag="OI501_RS29520"
                     /old_locus_tag="OI501_29525"
     CDS             4668..4994
                     /locus_tag="OI501_RS29520"
                     /old_locus_tag="OI501_29525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            5020..5565
                     /locus_tag="OI501_RS29525"
                     /old_locus_tag="OI501_29530"
     CDS             5020..5565
                     /locus_tag="OI501_RS29525"
                     /old_locus_tag="OI501_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3599..3675)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3864..4261
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1481..3012)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg cgttcctagc
       61 atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc gatgtaatgg
      121 tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg taggtctttc
      181 taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg aagtagtaat
      241 gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta tgcctgacgt
      301 taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga caacctgctg
      361 gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac cataatacct
      421 gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg cggcgatgtc
      481 caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga acgcgataat
      541 ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc cctgactggc
      601 gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt cgtgcgttct
      661 gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc gtccagacca
      721 agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg aacgcgtgca
      781 gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag tattgcttcc
      841 cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag acgggactca
      901 atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt tgctatacat
      961 gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt tagcccctct
     1021 tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta actattgaaa
     1081 ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca acaggggaac
     1141 ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa ttgaaaccca
     1201 gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt ttgtatctgc
     1261 cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg gttcgccgcg
     1321 aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc cccgttcgcg
     1381 acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat ccggtttcag
     1441 cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg gaccgtcata
     1501 gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt gctcgtgcgc
     1561 ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg ctgcagttgc
     1621 ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag cgccgcgttc
     1681 tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc ccactcgtcc
     1741 agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg tttttccagc
     1801 tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga ccgcccgttc
     1861 ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac cagaaaacgt
     1921 ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt cgacagcgct
     1981 gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg ataccacaac
     2041 accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag ccatgtccgc
     2101 ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat accctgctcg
     2161 tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc tctgacagac
     2221 ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg ctgcgctttc
     2281 gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg ctcccttaag
     2341 tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg tggtgcgcac
     2401 gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt taagctcccc
     2461 tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct gcttcgtttt
     2521 gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg ggtcgtgcag
     2581 ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt ctgcccggtc
     2641 gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca gaaaattcag
     2701 ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt ctttatcgag
     2761 tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc cgggcggtaa
     2821 atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg gcgaggcgtc
     2881 gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt cgcgctggcg
     2941 gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc gcggatgaaa
     3001 cttaacgatc atcgtctgcc cccttttcca gcacggcatg ccgcagccgc tcgagttcgg
     3061 catcaatggc catcagcgcg gccaccacct gaacccggtc agcgcctgac cactggccgc
     3121 cgttaatttt ccgggcgatc tggttgaggt tgttccccat cccggcaagc tggcgcagca
     3181 aaaccggatc gatggacggc agccgcaacg accgcgccgg gcgcgtatcg agacaggttt
     3241 cccgcatcca caccgccagt tgccttccat tgcagcgctc cagcagctgc tggtactccc
     3301 catcggtcac ccggatacct aaaaaacggg tacgcttaac cgccatcctc tcctccgata
     3361 tccgcaaaat gtgaccgggc tgaacgctca agctctgcca tctgctcccg gcggtaatct
     3421 tcctcctgtg cctgacgcat cagatttgcc atcactgccg gatgccagga tctttttttt
     3481 acgacgagcg gcttcttctt cggcttctgc attatccctc cttcgcaaac gccggacggc
     3541 acggaaaagt tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc agtggtgtta
     3601 gcgggtttcg gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta tactggctta
     3661 ctatgttggc atgagagcac gattcatgaa agtgcctcat gtggcaggtg tgatatgtgg
     3721 ctcaggagcg tccgcaatac catgcacgga cagatgaaat gacgcctcct cgctcactcg
     3781 gtagctgcgc tcctgccgtt cggctgcggc gggcgtgacc gactcacgga taacgggata
     3841 aagcaaaggc gctcaaatca gaaagggccg gagaaaagga tgcggcgttg ccgtttttcc
     3901 ataggctccg cctcccctga cgagcatcac agaaatggac gctcaagtca gaggaggacg
     3961 aaacccgaca ggacttaaag atgccggtga tttctctccg gcggctccct cgtgcgctct
     4021 cctgttccga ccctgccgtt taccggatac ctgttccgcc tttttccctt tcgggaagcg
     4081 tggcgctttc tcatagctca cgctgctggt atctcggctc ggtttaggtc gttcgctcca
     4141 agctgggctg tatgcacgaa ccccccgttc agcccgactg ctgcgccctt tccggtaact
     4201 atcatcttga gtccaacccg gacggacacg caaaaacgcc actggcagca gccattggta
     4261 actggatata tacgagagag atattgagag gtcttgaagt gggtgcctaa ctgcggctac
     4321 actgaaagaa cagttttggt atctgcggtc tcgtataccc agttacccgg ttaagcagtt
     4381 ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacagtg
     4441 caagagatta cgcgcagaaa aaaaggatct caagaagatc ctttaaatga tcgtcagttc
     4501 tgttatcccc ggagctccat ccatcccacg gaaaaacacc cgccactgat gccctcagcg
     4561 cactgcacat catgtacaaa ccactccctc cctccttttt tattgcgttt cttaatgcgc
     4621 aatggtagct ttgtgcgcaa caaaaaacgc aatagcaagg gggggtaatg agagaaagaa
     4681 caaatatcat gctagatata ggattaaagc aatccttaca acggctttcg aataaaacca
     4741 aaatgagtct aagtgatctt attaaccaga gattatcagg ttcactttca gttgaagaac
     4801 gcattggcga aaagcctgaa tggatgagaa accttgatcc ctatctcgcc aatcactttt
     4861 caaatattga gttaccgctc ccttcaccat taaaagaata ccaagaaaaa atggatgaga
     4921 tagattcaat tattaatgac atgatggttc taaaggaata tttcagagct aagttttacg
     4981 acgaaataaa ataggcacgc tatacggagg cggagaatta tgttttttaa acggtattta
     5041 acggagaagg aaatatacaa ggcagcactc ccaattgtta ggcttcaagg gagagttaac
     5101 acttcattac gttttttatc caaatactca gatgtaaaca aaaatggatt aattgcattg
     5161 catcttaatt tattacattt tatctcatac aacatacacg gtgtagagtc tctgaaaatc
     5221 actcaagcag gcataaacaa agctgtacaa caaagccctc acgcagatga aataaaaaat
     5281 attgcgcagg caataggacg caaaggacct gagtacatcg aaggattaat tagaaaccca
     5341 tgggggaaga aaaatgataa tccactaata tttataatga gcattgaaga aagtataaaa
     5401 gaaaccctta actcaggtta tatgttatat agagtagctc ccataactga gggtgaagca
     5461 gctgctttca tggatgttct taaacaatta ttattatttg aggataattt tcgccttttt
     5521 ggtagagact tggtttataa aatagaaaaa ttaaaaatca gataacaaag tatggtgaac
     5581 gaaaaatgaa agagag
//
