LOCUS       NZ_CP109954             7136 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Escherichia coli strain NC22 plasmid p1, complete sequence.
ACCESSION   NZ_CP109954
VERSION     NZ_CP109954.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31396625
            Assembly: GCF_029635575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Tan,M.
  TITLE     Identification, biological characterization, and genome analysis of
            Escherichia coli O24 isolated from duck
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Tan,M. and Tan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2022) Institute of Animal Husbandry and Veterinary
            Science, Jiangxi Academy of Agricultural Sciences, 1738 beida road,
            liantang, nanchang, Nanchang, Jiangxi 330200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.1-b1676
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 190.0x
            Sequencing Technology             :: nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029635575.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 06:03:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,292
            CDSs (total)                      :: 5,169
            Genes (coding)                    :: 4,890
            CDSs (with protein)               :: 4,890
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 1 of 279
            Pseudo Genes (frameshifted)       :: 104 of 279
            Pseudo Genes (incomplete)         :: 176 of 279
            Pseudo Genes (internal stop)      :: 57 of 279
            Pseudo Genes (multiple problems)  :: 52 of 279
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109954.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NC22"
                     /isolation_source="brain"
                     /host="duck"
                     /db_xref="taxon:562"
                     /plasmid="p1"
                     /geo_loc_name="China: Nanchang"
                     /collection_date="2022-08"
     gene            complement(join(5862..7136,1..225))
                     /gene="csa"
                     /locus_tag="OI124_RS23795"
                     /old_locus_tag="OI124_23715"
     CDS             complement(join(5862..7136,1..225))
                     /gene="csa"
                     /locus_tag="OI124_RS23795"
                     /old_locus_tag="OI124_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_024216192.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDDYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            464..754
                     /locus_tag="OI124_RS23800"
                     /old_locus_tag="OI124_23720"
     CDS             464..754
                     /locus_tag="OI124_RS23800"
                     /old_locus_tag="OI124_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(746..1024)
                     /locus_tag="OI124_RS23805"
                     /old_locus_tag="OI124_23725"
     CDS             complement(746..1024)
                     /locus_tag="OI124_RS23805"
                     /old_locus_tag="OI124_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1146..1568
                     /locus_tag="OI124_RS23810"
                     /old_locus_tag="OI124_23730"
     CDS             1146..1568
                     /locus_tag="OI124_RS23810"
                     /old_locus_tag="OI124_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2052..2285)
                     /locus_tag="OI124_RS23815"
                     /old_locus_tag="OI124_23735"
     CDS             complement(2052..2285)
                     /locus_tag="OI124_RS23815"
                     /old_locus_tag="OI124_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2292..2852)
                     /locus_tag="OI124_RS23820"
                     /old_locus_tag="OI124_23740"
     CDS             complement(2292..2852)
                     /locus_tag="OI124_RS23820"
                     /old_locus_tag="OI124_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2887..3567)
                     /locus_tag="OI124_RS26500"
                     /pseudo=""
     CDS             complement(<2887..3567)
                     /locus_tag="OI124_RS26500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3530..3853)
                     /locus_tag="OI124_RS23830"
                     /old_locus_tag="OI124_23750"
     CDS             complement(3530..3853)
                     /locus_tag="OI124_RS23830"
                     /old_locus_tag="OI124_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3921..4112
                     /locus_tag="OI124_RS23835"
                     /old_locus_tag="OI124_23755"
     CDS             3921..4112
                     /locus_tag="OI124_RS23835"
                     /old_locus_tag="OI124_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(<4267..4452)
                     /locus_tag="OI124_RS26505"
                     /pseudo=""
     CDS             complement(<4267..4452)
                     /locus_tag="OI124_RS26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            4781..4894
                     /locus_tag="OI124_RS26510"
     CDS             4781..4894
                     /locus_tag="OI124_RS26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_213849828.1"
                     /translation="MHEPPVQPDRCALSGNYRLESTPVRHDKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            complement(5110..5265)
                     /gene="csl"
                     /locus_tag="OI124_RS23840"
                     /old_locus_tag="OI124_23760"
     CDS             complement(5110..5265)
                     /gene="csl"
                     /locus_tag="OI124_RS23840"
                     /old_locus_tag="OI124_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            5295..5834
                     /gene="csi"
                     /locus_tag="OI124_RS23845"
                     /old_locus_tag="OI124_23765"
     CDS             5295..5834
                     /gene="csi"
                     /locus_tag="OI124_RS23845"
                     /old_locus_tag="OI124_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104681840.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(4199..4285)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          4309..4853
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.978"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1946..3540)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3913..4115)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.39e-10"
ORIGIN
        1 cccattatca tcaacataag gcggcacatc cggattattg gttttactcc atccccaccc
       61 cgtctgactt ccatgttcac gacctccatt accagagcct gaaccaccac cccagtgaac
      121 cccactatta ctattattat ttccaccttg ctgattcaga ttcgcccctg ttccccccat
      181 agactcacca gcagttgggc catatccact tagttcttta gccataaatt cctctttgat
      241 aattaaaaca ataaattaaa aacagtatac tgtacatata accactggtt ttatgtacag
      301 caaaacctac tactcaccat tgtccatgtc aagagcatgg attttcattt ttgctataag
      361 gatcacgcta tggggaggca ggcattgaga acgtcgaaac agaacaccgg agcaaatcag
      421 gatgagatat aaaactgttg gatcatgaaa aacggagaac gatgtgagca aatcaccccg
      481 ccataaactg aacaaaacag acaaacgact tctcgacacc cttgttgctg ccggatatga
      541 gcatgacaaa gcccgtgacc tcatccagaa acaggtttac acgctgacac tggctgatca
      601 gcgtcatgtg gtcagtgaaa tcagtaatgg tgtgaatccc acccaggctt actcggcggt
      661 ataccaggca agacgcattc gcctcgcccg taaatatctg aacggtaaaa aggttatgga
      721 agaaaccggg gaaaatacgc ccccatcaac gtaaggattt cttttgccgc tccagagact
      781 ccagtttttt acgcaaatcc tctctttttt gggcatctct ggtgccagcc agctctgctc
      841 tcaactcatc gatctgaagt tgtatcttca gtctgttact gaacattttc tgtctggcat
      901 taacatccgc aacaatgccg ttttttgtct tctcggcctt ttgttgaaaa acattgctgt
      961 ccgcatgact ggcaaccgaa gcagaaagaa cactaaaaag caggactggc acacattttt
     1021 tcacgggatt attcctgact cattgaccat caaatcacat tgggagtaaa ccgacgtatg
     1081 ataagagata ctcttcggag atataactcc ctgagtatca agattaaaaa cgcaaggaga
     1141 tgtttatgag atctgccgct accaggctgc ttctgatacc tctgataaca gcaacaatag
     1201 ctcttacagg atgcacccca aagaccagcc tggaacgaca tacccgacat tatgtttatg
     1261 cttcagatga tggatttgat cctaacttct atacccagaa agcagacacc atacgtatga
     1321 tgctcccgtt ctttcagcag ttccgggata tggggatgaa agacaaagca gccggagtat
     1381 cagcagaaac ggcacagcaa cgtgcaaaag aattccactc agaaaaattt tttcactcac
     1441 tccagagcac aacaaccttt gctggcagaa aatacacaaa cagcgatatg ccttcgctga
     1501 aaaaaatgaa actaatggca gacaccattt ctgcggttta tctcgatgga tacgagggca
     1561 gacagtaagg gatttaccat aatcccttaa ttgtacgcac cgctgaaatg cgttcagcgc
     1621 gatcacggct gctgacaggt aaaaatggca acaaaccacc cgaaaagctg ccgcgatcgc
     1681 acctgataaa ttttaaccgt atgcatagct attcagccat gtgaataacg ctggttttgc
     1741 ctgcgtaaac ctcatgacac tgtttttttt ccatcttttc agttgatgac atacgcagac
     1801 atcgcgggat gaggctgagg aatgagcgcg atctggcaaa gaggcaaaac acagcaacaa
     1861 aaacgacacg ccagaatcgc gcccggatgc gtttttaacg cgttccggta ccatctggca
     1921 acctcccgga acaactcacc gtcacatccc cattgacggg ccacgccata cctgtgcttc
     1981 ccgttcctgc tcttcatgcc aggaccgcgc acgctcccgt tccaggcgtg cctgcctttc
     2041 ctgttcatcc cttatctgct gttcgtgata aataaccgac tcaagcggtc cacctgcccg
     2101 gctaatctct gcacctgctg actcaagtcg tcgcactgtt ccctcagttg cccgttctcc
     2161 tgtcgtgtca gctcgaacat atgctgcaaa tccgtgaagg cgctctccca gtctttcagc
     2221 cgctgcgtat agtcctgctg caattgctct aaggcgttca gtaagtgcat ttccagctct
     2281 gtcatactca cttactccct gaccagtctt actgcgttct tcttctccac cgtccagttg
     2341 ttttccccct tcaccccgga cggcagcacc agaaatttcc cgttcctgcc ctcgtgatac
     2401 gtcacacccc atgttttttc ccggagtttc gccagcgtct cttcctggtc cctgatagcc
     2461 aggatgttcg ccgcaatccg gctttcctgc cactgaatca gcccccatga cgccagaaaa
     2521 atcccgcccg tgacgcaaag cgccgtcagc gacgggtaca gtatccgccc tttgatcagc
     2581 ttccagagca gctcttcctg ccgccgggcc agttcgttct ctgtggcgct gaactgcgcg
     2641 ttcacggcac tgttcagcgt ctccagttgt tctttcaccg ctgctgtgtg tgcgctgata
     2701 gcgtctctga ttttctgccc gtttaagttc agttccctgt ctacagacgc ttcgagcttc
     2761 ctgaactcgc tgttcagcat gttctctgta gagacggcac gctctttcag tttcttctcg
     2821 aagtctgtcc ccatttgtaa aagattgctc atacagcgcc cctttcagcc tgagattacg
     2881 cccgccctcc gggtcggcga tactgatact gctcctggtt gtcctcacaa cctcaaaacc
     2941 tgccgctgta agcgcctcag tgacatcctg acgcgttttt agcgctccgg catggtaaag
     3001 agcctccagt cccctcgtaa tcgcttctgc ggcctcctgt ttcgctttcg gcagattatt
     3061 cggggtgaca agtgtccgcc tgttctccgg tgcgttcggg tcgtgcagcc cgtaatggtg
     3121 attcaccagt gtctgccagg cattgattcg cggacggtcc gcccggtcgt aatagggctg
     3181 gagccgtttt ccgctcgcca gctccatatt cgggatgaca aaattcagct caagacgccc
     3241 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcaagac cgggcatcag
     3301 tacccgctca aagctctcca tcaccctttc acgctctccc ggtggcaggg tctgctctgc
     3361 aaaagacaga acccccgagg tgtatttttt cgcaaacggc gtggcatcga tgagttcccg
     3421 tacctcttcg ggagcacccc gcagaactct caccccttcc cggttacgct cccggcccag
     3481 caggtaatca accggaccac tgccaccgcc ttttcccctg gcatgaaact taactatcat
     3541 cccgttctcc ctgtttacgg acctcatccc taagctcact cagttcacgt ccgatggcca
     3601 tcagtgcagc caccacatga acccggtcat gccccgacca ctgtccgctg tttatcttcc
     3661 gggctatctg attcaggtta ttgccgaccg aagcgaactg gcgcaacagc ggcggtgcca
     3721 gtgtcggaag acctgacgtt ttcgatggcg gtgcccccag gcagacctta cgcatccatg
     3781 acgcaagttg ttttccctca caacgtgcca gcagccgcgc atgttcctca tccgtgaccc
     3841 gtatcgtgag catcctttcg cgtttcaccg gtatcattaa aaacctccga cagactcccc
     3901 acacatggag aaacagaact gtgactaaac aggaaaaaac cgcccttaac atggcccgct
     3961 tcatcagaag ccagacgctg accctgctgg aaaaactgaa tgaactggac gtcgacgacc
     4021 aggctgacat ctgcgaagcg cttcacgacc acgctgacga gctttaccgc agctgcctcg
     4081 cacacttcgg ggatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4141 ctggcctgtg agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgtta
     4201 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     4261 taatcgtgta gcatcagtga ggattgtatg aaaagtgcac catgccgggt gtgaaatgcc
     4321 gcacagatgc gtaaggagaa aatgctcgtc caggcacttt tccgcttcct cgctcactga
     4381 ctcgctgcgc tcggtcgttc gactgcggcg agcggagtct gcttgctcaa aaacggcgat
     4441 acggttatcc acagaatcag gggataaggc agaaaagaac atgtgagcaa aaaggcaaag
     4501 ggcaaaaaaa gccgcgtcgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     4741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4801 gcccgaccgc tgcgccttat ccggtaacta tcgtctcgag tccaccccgg taagacacga
     4861 caaagcgcca ctggcagcag ccattggtaa ctgaaaagtg gatttagata cgcagaactc
     4921 ttgaagttga agccttatag cggctacact gaaaggacag catttggtat ctgtgctcca
     4981 cttaagccag ctacccggtt aagcagtccc caactgactt aaccttcgac taaaccgcct
     5041 ccccaggcgg ttttttcgtt tacagggcaa gagactacga cgatctgtaa aggatctcaa
     5101 gaagatcctt tacaggctac cactaccacc acttcccata ctcactccgg taacagtgga
     5161 agatggtgaa acagccccac ctccagtatc tctgacattg ttgacttggc atgctgcaag
     5221 cagcattatt cctagtgaaa tggcacaaat aataattttt ttcatatata cctttattat
     5281 aatcctatag gtcaatgatg attgatgaac attcaataga tatagacaat agaaaagcca
     5341 ataacttatt gtatttattt atggtaatcg gagttatacc tttactctgt atactagccg
     5401 tttactatac aaatccagac aatctgttct tgcacacaat agctaccagt actgaaaaca
     5461 tcccatcaat aacctccgcc tacaacccat taatgacaaa ggttatggat atttattgta
     5521 aaacagcgcc ttcccttgct ttgatattat ttatcctgac atttaaaacg agaaagctaa
     5581 taaaaaaaac aaaccgaaat gctgtactta gaagttgctt attaagtcca tttgtctgtg
     5641 ctgttttttt gtatttactc tgtttccgaa accttgagct aacgacagca ggaaggccag
     5701 ccaggctaat gaccaacaat gatgcaacac tattgctatt ttatattggc ctgtacttaa
     5761 tcattttttt cataagctat ttcacgctat tcacaccagt taccacgttt aaattattaa
     5821 aagaaaggca gtaaccctgc cttcctttta cccggtattt attaatgcgc gggtttaatg
     5881 atttctttat ttagtgcatc tgcaaacttg tcatcaatta atgcacctac aactgctgcg
     5941 agtagaatac ctacgatacc gacagcaata gcaggagccc caagagataa ggcatatgcc
     6001 cctaatgtgg ctgaaaaaac acccagggcc acagcagaag cgatgccact gagcacccag
     6061 gactccacct ccagcatcag aggtccccag ttcccggttt catagccctc gatgcttttc
     6121 tcccggacct tctcaacctt catcaccaca tcggcaaatt tgaaagcctt gctgatatta
     6181 cccagtttat ttgccatatc ctgagcatca atacttcgcc agacgttcgc cagtgcatca
     6241 ctgtcggccc tgttaaattt aaaccccgga ttagacaaaa ctttatttaa tgacgccatt
     6301 gcatcatcgt agctacggat agttttcccc tggaaatttt tgacatcatc agcaatttct
     6361 cttgctaacg acttataatc atctccaaga taatcaccga ccttattacc tatatcagag
     6421 ataacttcag cggctttatt aagtacatca ctctgaatcc ggtcacggtt catactgaaa
     6481 gaataaaaca catcataagc agctttttta gcttgatcct gtcgttgagg ctcttcatta
     6541 ttaaatccta atgcctcata tatctcttcc ggggtcttat ggtcgacttt atccataata
     6601 ccagccttag ctttttcact ccctacataa ggacgaacag ctgttctagt tacagataaa
     6661 acattaccat aatcatcaag caccactaaa tagtggaact gttcaacatc aacatcaaat
     6721 tttctcacat taggaggcaa acgatcccac gcatcttcag cctgttgttt tgctttttcc
     6781 tgccgctgag attcatcttt attaaaacct aacgcctcat atatctcctc aggagtttta
     6841 tgattgattt tttccataat acccgcctta gctttttcac tccctacgta aggacggaca
     6901 cctgttcgaa gaataccgat agctctacca ttatcattaa gcgttacacg atatttaaaa
     6961 ccatcgatac tcacatcaat ctctcgcggt aagttgtttt tatcccactt acgagcaact
     7021 tcatcattag gattttcagg gatatagccc ccctgaacat cactatttcc gccagcccca
     7081 ggaactccat atacaggcgt ttttaccaga ccatttgtga tggtaatgcg cacctg
//
