LOCUS       NZ_CP109928             1240 bp    DNA     circular CON 05-JUN-2024
DEFINITION  Escherichia coli O125ac:K+:H10 strain C 236-04A plasmid pP10A_4,
            complete sequence.
ACCESSION   NZ_CP109928
VERSION     NZ_CP109928.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30931041
            Assembly: GCF_025946545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O125ac:K+:H10
  ORGANISM  Escherichia coli O125ac:K+:H10
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Meski,S., Struve,C., Mirsepasi-Lauridsen,H.C., Petersen,A.M.,
            Jelsbak,L., Skovgaard,O. and Krogfelt,K.A.
  TITLE     Complete Genome Sequences of Extraintestinal Pathogenic Escherichia
            coli Clinical Isolates from Danish Ulcerative Colitis Patients
  JOURNAL   Microbiol Resour Announc 12 (2), e0118622 (2023)
   PUBMED   36715532
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Skovgaard,O., Krogfelt,K.A., Jelsbak,L. and Meski,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Department of Science and Environment,
            Roskilde University, Universitetvej 1, Roskilde 4000, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method :: Flye v. v2.9; Minimap2 v. v2.24-r1122; Miniasm v.
            v0.3-r179; Racon v. v1.4.3; SPAdes v. v3.15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology :: Oxford Nanopore MinION; BGISEQ-500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025946545.1-RS_2024_06_05
            Annotation Date                   :: 06/05/2024 03:53:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,899
            CDSs (total)                      :: 4,782
            Genes (coding)                    :: 4,578
            CDSs (with protein)               :: 4,578
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 58 of 204
            Pseudo Genes (incomplete)         :: 141 of 204
            Pseudo Genes (internal stop)      :: 38 of 204
            Pseudo Genes (multiple problems)  :: 30 of 204
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109928.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli O125ac:K+:H10"
                     /mol_type="genomic DNA"
                     /strain="C 236-04A"
                     /serotype="O125ac:K+:H10"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /culture_collection="SSI<DNK>:C236-04A"
                     /db_xref="taxon:2990470"
                     /plasmid="pP10A_4"
                     /geo_loc_name="Denmark:Copenhagen"
                     /lat_lon="55.68 N 12.59 E"
                     /collection_date="2007"
                     /collected_by="Statens Serum Institut"
     gene            720..1229
                     /locus_tag="N8A72_RS24385"
                     /old_locus_tag="N8A72_24385"
     CDS             720..1229
                     /locus_tag="N8A72_RS24385"
                     /old_locus_tag="N8A72_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023206179.1"
                     /translation="MIVASEHQAKLRQKEIQEIDKKASKEHEEAKKNSNFTQTYPKGWQ
                     RIRELVRDKQGAAAIGLYSFFAEHIDPTCGAVVCDQQFLANQMGVSTRTIRRWLDYLEE
                     KGALVRIPVAGKVCAYALDPHEVWKGYNTSKDYSAFVTKTLVNKDGEIKRRIMAMFSGE
                     ESGQEQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ttgacggctc ggaacgaccc ccaccctcag aaagggggat ggttcgccac agcgagccat
       61 ccccataata agccgccctt taagcagtag atcccataaa taaataaaac agcacatcac
      121 ttttgtaatg tgcggcattc ctttgggact aaaagaagtt agccaggaat gtaatacaga
      181 aactttaccg acaaggaggt catcatgatc acgtcaacaa tcatcacaat catcggctta
      241 attgggagtc tcataacgat ttaccgagag atatacccga aggataagca ctaattagcc
      301 agtatacact ccgctagcgg agtgactggt tcagggcttc gccccgaaac ccctgaaaac
      361 cgtcaatcat ggtgatggct cactacgcga gccatcacca gatacaaggt taaacgggca
      421 ttttgatcgt caaggtcaag ccgctaacgc ggtcgcctat ggcgaaccct gaccctcaaa
      481 atgcccgacc ggagggaggg agctaccttg aactttggtg ttgtctgttg gttttcatag
      541 aagagaatag agataaataa taagttattg atttttatag ttttggtttt ttctgatagg
      601 acacagctgg tgtccgcacg tgaggacaca gctggtgtcc gcatgtggct ggacatagga
      661 caccacaagt cctatgatgg gacaaaaatg tcctatcaaa aagacaccga gggataaaga
      721 tgatcgtagc ttcagaacac caagcaaagc taaggcagaa agaaatacag gaaatcgaca
      781 agaaggcatc taaagagcac gaggaggcaa agaagaacag taacttcact cagacatacc
      841 caaagggctg gcaacgcatt agagagcttg tgagggacaa acagggtgct gccgctatag
      901 gtctttactc atttttcgct gaacacatag atcctacatg cggtgcagtg gtctgcgatc
      961 agcagttttt agctaatcag atgggtgttt ctactcgaac aattcgtcgt tggttagatt
     1021 atttggagga gaaaggtgct ttggtaagaa taccagttgc tggaaaggtt tgtgcttacg
     1081 ctttagatcc tcacgaagtc tggaagggat ataacacatc aaaagactac tcagcctttg
     1141 ttaccaagac attagtgaac aaggatggag aaataaaacg ccggataatg gctatgtttt
     1201 ccggagagga gagcggtcaa gagcagtagc gaagcgactc
//
