LOCUS       NZ_CP109828             2883 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Serratia marcescens strain SMBC31 plasmid pSMBC31-2K, complete
            sequence.
ACCESSION   NZ_CP109828
VERSION     NZ_CP109828.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31283467
            Assembly: GCF_025913695.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Tang,B., Zhao,H., Li,J., Liu,N., Huang,Y., Wang,J. and Yue,M.
  TITLE     Detection of clinical Serratia marcescens isolates carrying blaKPC-2
            in a hospital in China
  JOURNAL   Heliyon 10 (8), e29702 (2024)
   PUBMED   38660286
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Jul-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025913695.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 15:55:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,194
            CDSs (total)                      :: 5,065
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 13 of 47
            Pseudo Genes (incomplete)         :: 31 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 6 of 47
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109828.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SMBC31"
                     /isolation_source="blood"
                     /db_xref="taxon:615"
                     /plasmid="pSMBC31-2K"
                     /geo_loc_name="China:Zhengzhou"
                     /collection_date="2021-10"
     gene            936..1679
                     /locus_tag="OHY99_RS25980"
                     /old_locus_tag="OHY99_25985"
     CDS             936..1679
                     /locus_tag="OHY99_RS25980"
                     /old_locus_tag="OHY99_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094543600.1"
                     /translation="MTIQEVKKTRVRTKKENDKLTEYKMNPFVFEKELKIETKTRNLTV
                     RKGTELVSKDNSDESESYLTNIIQRKEVDKEEFIKLFTSQIKVYFDLTKTAYKIFLIVL
                     SLYQKEIGKDYVLLTCKKAQNIAKTLDFELSSPIFYRGIKELIEKKIIAKSVDKIVFYI
                     NPAIFFNGDRARFVTEVIKKKEEIEKNQLLQELEDTSKNKDFDVNETDDSVENLDLMIA
                     RLQKKKEELERKEKFGNWQDLEDAS"
                     /name="hypothetical protein"
     gene            complement(1696..2802)
                     /gene="mobV"
                     /locus_tag="OHY99_RS25985"
                     /old_locus_tag="OHY99_25990"
     CDS             complement(1696..2802)
                     /gene="mobV"
                     /locus_tag="OHY99_RS25985"
                     /old_locus_tag="OHY99_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791318.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_094543598.1"
                     /translation="MYSIIRKQSHKKTAIQAVHNHNMRIVIEENVNSKKSNLNEILIGT
                     ENTRSDIMSYIKNNDIVIRNKDTVIFNEFVLTASPEFFFNNKDGSKKTKSEYNDNLKEW
                     VKTQIEFLEKENYGKCVNAVLHLDESSPHIHALILPIVDNKLNNKSFWRGKNSYGRLQD
                     IYNASNSKFGLKRGEEKSKTLVDHTTLKDYRELIKQDLEEEKEFYNELSKDILDIPPRK
                     NLIGLEKKYSAEEVKEIASATFKKLNRQRRRAKFRAKKSDEKADAATSEFYKKKKETSK
                     IEFFYNENQKQLEKTIVENSTLKTQNLELKQSQSDLELVKKFLPDELQSLVVRARELAS
                     GGDSGGTKHTAEPTPTADLTTYKRPKYK"
                     /name="mobV"
ORIGIN
        1 gtggcgttaa atgccacctg tgcagctgtg taaggttttg acgttgaacc ggtgcaaacc
       61 ggttcggggc ttgggtgggc tatgcccccc aacgaacgac tgagcgaagc tcggtctagt
      121 gagtgataga ccgagaaagt aaactcaacg tatatattta aagttatcac tttaatatat
      181 tctgtcaaat agctaccgcc tcgctttgca gctcgttgct aaatcttttt taaagcgaaa
      241 gtaaactttc actttaaaaa agattgaata taaaaatgca aagtttccat atgtagactg
      301 aagtctacga tcagaaactt tttctactgc atgtaggaat gggtaaatgc aggctggtag
      361 cagctagcat tggggtgggg ggaccctgtg atgaacaaga gtccttgttc gaacaggtgg
      421 ggggaacggg tttaggggtt ccccctatgt agctgtagaa cgctctgtaa cgctctgtga
      481 cgggtttttg tagttactta aaccgtgaga gtccacgtac agcttgctgt atgtggattc
      541 gaggggttgg ccagcccggc gagggcggtt ttagattttg gttttactct tagatcttaa
      601 tatttattaa ttcttgcaga aaaactcaaa cactaaaact cctctctaag ccttggtacg
      661 tctgtctttg ctcccttttt tcaaaaaaac aaaaagtaca aagttttata ctctacagtt
      721 acttttttaa ggatagagta ataagtttta tactctagag taataaactt tatcgtttta
      781 cccccattaa aacgataaac acactaaaat ttctagaatt ttatttatcg ttttaactat
      841 caaaaacgac ttttttttat cgttttctat tgacatgtcc aaaggatcgg ttataacact
      901 aatatcaagt atctaaattt aaagaggaat aagttatgac aatacaagaa gtaaaaaaaa
      961 caagagttag aacaaaaaaa gaaaatgata aattaacaga atataagatg aatccttttg
     1021 tttttgaaaa agaactaaaa attgaaacaa aaacaagaaa tttaacagta agaaaaggta
     1081 ctgaattagt aagcaaagac aattcagatg aatcagaatc atatttaact aatattattc
     1141 aaagaaaaga agttgataaa gaagaattta taaaattatt tacatcacaa ataaaagtat
     1201 atttcgatct aacaaaaaca gcatacaaaa tttttcttat agttttgtct ttatatcaaa
     1261 aagaaatagg taaagattat gtgttattga catgcaagaa agctcagaat attgctaaaa
     1321 cattagattt tgagctttcg tcaccaattt tttatagagg tataaaagaa ctaattgaga
     1381 aaaaaattat tgctaaatct gtagataaaa ttgttttcta cataaaccca gcaatttttt
     1441 tcaatggtga tagagcaaga tttgtaactg aagttataaa gaaaaaagaa gagatagaaa
     1501 aaaatcaact tcttcaagag ttagaagata catcaaaaaa taaagatttt gatgttaatg
     1561 aaacagatga tagcgttgaa aatttagatt taatgattgc aagattacaa aagaaaaaag
     1621 aagaattaga gcgaaaagaa aaatttggaa attggcagga tctggaagat gcatcataaa
     1681 agcccgtgag ggcttttatt tatatttagg tcttttgtag gtagttagat ctgcagttgg
     1741 tgtaggttct gcagtatgct ttgtaccgcc agaatcgccg ccagaggcca gttctctggc
     1801 tctgacgaca aggctttgca gctcatcagg taaaaacttt ttcacaagtt ctagatcgct
     1861 ctgtgattgc ttaagctcta agttttgggt tttaagtgtg gaattttcca caatagtttt
     1921 ttccaattgc ttctgatttt cattataaaa aaactcaatt ttcgatgtct ctttcttttt
     1981 tttataaaat tcacttgtag ctgcatcagc tttttcatca gattttttag ctctaaattt
     2041 tgctcttctt ctttgtctat ttagtttttt aaaagtagct gaagcaattt cttttacttc
     2101 ttcagcagag tatttttttt ctaaaccaat taaatttttt cttggtggaa tgtctaatat
     2161 atctttcgat aactcattat aaaattcttt ttcttcttct aaatcttgtt tgattaattc
     2221 tctgtaatct tttagtgttg tatgatcaac taaagtttta cttttttctt caccacgttt
     2281 taaaccaaat ttactatttg atgcattata tatatcttgt aatcttccat atgaattttt
     2341 accacgccaa aagcttttgt tgtttaattt attatctaca ataggtaaaa ttaatgcatg
     2401 tatatggggg cttgattcat caagatgcaa aactgcatta acacatttcc cgtaattttc
     2461 tttttctaaa aactcaattt gggttttaac ccattctttt aaattgtcat tatattcaga
     2521 tttagttttt tttgaaccat ctttattgtt aaagaaaaat tctggtgatg cagttaaaac
     2581 gaattcgtta aaaattaccg tgtctttatt tctaatgaca atatcattat ttttaatata
     2641 actcattatg tcagatctgg tattttcagt accaattaat atttcattca aatttgattt
     2701 tttagaattt acattctctt ctataactat tctcatatta tgattatgta cagcttgtat
     2761 agctgttttt ttgtgtgatt gttttctgat tattgaatac atttttattt ctccatatta
     2821 tttatacaac ccccattttt ttggataaat ccaatattat gagaatgtcg aatacattct
     2881 cat
//
