LOCUS       NZ_CP109827             5699 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Serratia marcescens strain SMBC31 plasmid pSMBC31-5K, complete
            sequence.
ACCESSION   NZ_CP109827
VERSION     NZ_CP109827.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31283467
            Assembly: GCF_025913695.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Tang,B., Zhao,H., Li,J., Liu,N., Huang,Y., Wang,J. and Yue,M.
  TITLE     Detection of clinical Serratia marcescens isolates carrying blaKPC-2
            in a hospital in China
  JOURNAL   Heliyon 10 (8), e29702 (2024)
   PUBMED   38660286
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Tang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Jul-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025913695.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 15:55:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,194
            CDSs (total)                      :: 5,065
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 13 of 47
            Pseudo Genes (incomplete)         :: 31 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 6 of 47
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109827.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SMBC31"
                     /isolation_source="blood"
                     /db_xref="taxon:615"
                     /plasmid="pSMBC31-5K"
                     /geo_loc_name="China:Zhengzhou"
                     /collection_date="2021-10"
     gene            join(5219..5699,1..203)
                     /locus_tag="OHY99_RS25945"
                     /old_locus_tag="OHY99_25950"
     CDS             join(5219..5699,1..203)
                     /locus_tag="OHY99_RS25945"
                     /old_locus_tag="OHY99_25950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264384022.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSVSLMHEDLKRLPGAVS
                     TETVQTLEPLARLRQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAQAFEQKAGKLDA
                     TISDLSRSLSGLKTSLSSMEQTAQQVASLPGKLASAQQGMTKAADQLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWAKTIWSKATPKERELLKQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            239..1174
                     /locus_tag="OHY99_RS25950"
                     /old_locus_tag="OHY99_25955"
     CDS             239..1174
                     /locus_tag="OHY99_RS25950"
                     /old_locus_tag="OHY99_25955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264384023.1"
                     /translation="MKREGRHLSVRAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAIYRLSGAPTVRQQSHPSEPKRQPPQLPAQGLTAREHGRDYLKGRGIT
                     SDTIEHAEKAGMVRYADGGVLFVGYDRAGTAQNATRRAIAPADPVQKRDLRGSDKSYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKQA
                     ERVTVAGENEKNPDAQAKADAGHQKQAQRVAKITGREVRQWTPKPEQGKDLADMNARQV
                     VEIERKRQAEIEAESAAQAPEGYDGPSFGG"
                     /name="hypothetical protein"
     gene            1245..2171
                     /locus_tag="OHY99_RS25955"
                     /old_locus_tag="OHY99_25960"
     CDS             1245..2171
                     /locus_tag="OHY99_RS25955"
                     /old_locus_tag="OHY99_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_119589521.1"
                     /translation="MAGSIFDDLQRQGEPDLSGFQWLDLRHAVEQAPEPLDFVLPGFKS
                     GTVGALVSPGATGKTMLALQAAVTVSGGPDMLDFASMDTAWRRTAGRVVFLTGEDPADV
                     LNGRFHAIGQRLNPAEREAMFENLSVAPLVGRGADVMTFEWRRWIAGVTCDARLVVIDT
                     LRRFHQLDENDGGHMAGLLAYMEQLCRENSTTVLFLHHTSKAGALNGGDAQQASRGSSV
                     LTDNARFQANLVGMTPEQAKQFDVDENCRRNFVRLSFPKINYSAPIPDQWFRRRDGGLL
                     EPAVLERGTFKIAKTGSFQKGGDDERW"
                     /name="helicase RepA family protein"
     gene            2158..2910
                     /locus_tag="OHY99_RS25960"
                     /old_locus_tag="OHY99_25965"
     CDS             2158..2910
                     /locus_tag="OHY99_RS25960"
                     /old_locus_tag="OHY99_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="WP_264384024.1"
                     /translation="MSGGNAVTPTSTSAQARVMLYQPSQRPRLRSGDWTETSFGRCRIE
                     GRLGQRHADVVESILYCAEKRRDVSDGGIELLVDPARVRKTLSDSRYSFTQIEKLLAEL
                     RAATITIETPQFDFPIIGGLIDHVIPSPMTRPDPLTGGERNLWRVRLGVALVMLLEHDL
                     SLYYDPAPIARLQYGISQAVARHILSHKTEPLGGWYLDTAIMAVAGKSGSQAMRDGRRR
                     LKEDADELKALGLILDGDRIRKKRADDA"
                     /name="ABC transporter ATPase"
     gene            complement(3152..3619)
                     /locus_tag="OHY99_RS25965"
                     /old_locus_tag="OHY99_25970"
     CDS             complement(3152..3619)
                     /locus_tag="OHY99_RS25965"
                     /old_locus_tag="OHY99_25970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257411135.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSFEPSDGLI
                     ENLTDEEKTELQSYIETERQAAEKRTRQYFANSAASRIKEVADTIQGGDFEPSEAWAAD
                     TWAAIEALTKVMRKAGYPKPRKATQKAADAPMADQAGLPLGDAPEAPESAS"
                     /name="hypothetical protein"
     gene            4077..4463
                     /locus_tag="OHY99_RS25970"
                     /old_locus_tag="OHY99_25975"
     CDS             4077..4463
                     /locus_tag="OHY99_RS25970"
                     /old_locus_tag="OHY99_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_264384020.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPEVNREAWAALARTAANLNQIAHRLNAGDALPLAEVRAT
                     LDAFRRDLIGVKHDAEDAEDESES"
                     /name="ATP-binding protein"
     gene            complement(4654..5010)
                     /locus_tag="OHY99_RS25975"
                     /old_locus_tag="OHY99_25980"
     CDS             complement(4654..5010)
                     /locus_tag="OHY99_RS25975"
                     /old_locus_tag="OHY99_25980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264384021.1"
                     /translation="MQTLARRFVARSHGHVDLLGGQLPALGPRIAQPRRQGQAVGMLKL
                     LGRFDGAAGFPLAQPHFPVQPYPAGDDVYVILVRVLVAHGHPRRIVLVKPHMLHEVSRH
                     GFPLLGAQALARRQ"
                     /name="hypothetical protein"
     Typing          66..1168
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.957"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          4448..5691
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.843"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gcctctggcg gcaggcgctg gggctgattc tggccggggc cgtgggcgcg atgctggtag
       61 cgactgggca agtcgcttta aacaggctag tgccgccaag cgacgtgcag cagacggcgg
      121 actgggccaa aaccatctgg agcaaggcca cgcccaagga acgcgagttg ctgaagcaga
      181 tcgtcaatcg gcccgcgaac tagacccgac cgcctacctt gaggccagcg gctacaccgt
      241 gaagcgcgaa gggcggcacc tgtccgtcag ggcgggcggt gacgaggcgt accgcgtgac
      301 ccgccagcag gacgggcgct ggctctggtg cgaccgctac ggcaacgacg gcggggacaa
      361 tatcgacctg gtgcgggaga tcgaacccgg caccggctac gccgaggcca tctatcggct
      421 ctctggtgcg ccgacagtcc ggcagcaatc gcacccgagc gaaccgaagc gccagccgcc
      481 gcagctaccg gcgcaagggc tgacagcccg cgagcatggc cgcgactacc tcaagggccg
      541 tggcatcaca tcggacacca tcgagcacgc cgaaaaggcg ggcatggtgc gctatgcaga
      601 cggcggggtg ctgttcgtcg gctacgaccg tgcaggcacc gcgcagaacg cgacacgccg
      661 cgccattgcc cccgctgacc cggtgcaaaa gcgcgaccta cgcggcagcg acaagagcta
      721 tccgccgatc ctgccgggcg acccggcaaa ggtctggatc gtggaaggtg gcccggatgc
      781 gctggccctg cacgacatcg ccaaacgcag cggccagcag ccgcccacgg tcatagtgtc
      841 gggtggggcg aacgtgcgca gcttcttgga gcgtgacgac gtgcaagcga tcctgaaaca
      901 ggccgagcgc gtcaccgtgg ccggggagaa cgagaagaac ccagacgctc aggccaaggc
      961 cgacgccggg caccagaagc aggcgcagcg ggtggccaaa atcaccgggc gcgaggtgcg
     1021 ccaatggacg ccgaagcccg agcaaggcaa ggacttggcc gacatgaacg cccgtcaggt
     1081 ggtcgagatc gagcgcaagc gacaggccga gatcgaggcc gagagcgcag cgcaagcgcc
     1141 ggaggggtat gatggcccca gcttcggcgg ataagagcgc ctgtcagtcg gtgtgatgaa
     1201 cagaccgaca ggctaaccat catcaacctg tacaggaggc cgatatggct ggaagtattt
     1261 tcgacgatct acagcggcaa ggcgagccgg atttgtccgg atttcaatgg ctcgacctac
     1321 ggcacgcagt agagcaggca cccgaaccgc tggactttgt tttgccgggc ttcaaatccg
     1381 gcacagtggg cgcgctggtc agccccggtg ccaccggcaa aaccatgctg gcattacaag
     1441 ctgccgtcac tgtatccggc ggcccggaca tgctggactt tgccagcatg gacaccgcat
     1501 ggcggcgcac tgcgggtcgg gtggtgttcc tcactggtga agacccagcc gacgtgctga
     1561 atgggcggtt ccacgccatc ggccagcggc tgaaccctgc cgagcgcgaa gccatgtttg
     1621 aaaacttgtc tgtcgctccc ttagtaggac gcggcgcgga cgtgatgaca tttgagtggc
     1681 ggcgctggat tgctggcgtg acctgtgacg cccgattggt ggtcatcgac accttgcgac
     1741 gcttccacca gctcgacgag aacgacggcg gccacatggc cgggctgctg gcctacatgg
     1801 aacagctttg ccgcgagaac tcgacgacag tgctgttcct gcaccacacg agcaaggccg
     1861 gggcgctcaa cggcggcgac gcacagcaag cgagccgtgg aagctcagtg cttaccgaca
     1921 acgcccgctt tcaggccaac ttggtcggca tgacgcccga gcaggccaag caatttgacg
     1981 tggacgagaa ctgccgacgc aacttcgtgc ggctgtcatt cccgaaaatc aactattccg
     2041 cgccgatccc cgatcaatgg ttccgccgtc gtgacggcgg tttgcttgaa cccgcagttt
     2101 tggagcgcgg cacgttcaag atcgcaaaga ctggatcatt ccaaaaaggg ggcgacgatg
     2161 agcggtggta atgcagtgac ccccacgagc accagcgcac aggcgcgggt aatgctctac
     2221 caacccagcc agcgcccgcg cctgcggtct ggcgactgga cagaaacatc ctttgggcgg
     2281 tgccgcattg aaggtcggct tgggcaacgt cacgccgatg ttgtggaatc gatcctgtat
     2341 tgcgccgaga agcgccggga tgtttcggac ggtggaattg aacttctggt tgaccctgcc
     2401 cgcgttcgga aaacgctgtc agacagccgg tatagcttca ctcaaatcga gaagctgtta
     2461 gcagagcttc gggcggccac catcaccatt gaaacgcccc agttcgactt tcccatcatt
     2521 ggcggcctga tcgaccacgt tataccttcg ccaatgaccc gccctgaccc gctgacaggc
     2581 ggggagcgga atctgtggcg tgtccggctt ggagtggcgc ttgtcatgct tctggagcat
     2641 gaccttagcc tgtactacga ccccgcgccg atcgcccgac tacagtacgg tatcagccaa
     2701 gcggttgccc ggcacatcct aagccacaaa accgagccgc tgggaggctg gtatctcgac
     2761 acggccatca tggccgtggc cgggaagtcc ggcagccaag ccatgcgcga cggccggcgg
     2821 cgactgaaag aggacgccga cgaactgaag gcgttggggc tgatcctcga cggcgaccgg
     2881 atcaggaaaa aacgggccga cgacgcctaa agcgtggcac acccgcccga tagcgtggca
     2941 cacccgcccg atagcgtggc acagcggccc gaaacgtggc acacccgccc ggagtaagca
     3001 ggatattcag gatattcaga tcctgcgcgg cgcagcccgc gcttggtgcg cggtctggcc
     3061 ttgccatcgc cccccgcctt cggcgacccc ctccgggggt aaagggccag cggggagagg
     3121 gggtggaggc cggtttcccg gcctaaaccc atcaggaggc gctttctggc gcttctggcg
     3181 catcccccaa gggcaacccc gcctgatccg ccatcggcgc gtcagcggcc ttctgtgtcg
     3241 ctttgcgggg cttcggatag cccgctttgc gcatgacttt ggtcagcgcc tcgattgccg
     3301 cccaggtatc cgccgcccac gcctcggacg gctcgaaatc gcccccttgg atcgtatcgg
     3361 caacctcttt aatccgactg gcggctgagt tagcgaaata ctgtcgggtt cgtttttctg
     3421 ctgcctgtcg ttccgtttca atatatgact gtagttctgt tttttcttcg tctgtcagat
     3481 tttcaattaa gccgtctgac ggctcaaatg aatatacgtc aatagacccc atcatcttaa
     3541 cgatggcgcg gcgtttttcc ttgtcatacc cccggtaggc gagcacctga acccggcgac
     3601 cttggcgctt aaactgcatg gcggtttctc cgtttgactg tgtgattgta ttctagtctg
     3661 acagtcgtac gctgtcaagt gtttgcagtc ggtttctttt ttaatacgac tggcattctt
     3721 aaatgatcct tcactgtcgt attaaatagc gattgacact ctttcccgat tccgatacta
     3781 tagtggtatg gtatcacttc gccccgtcgc tttgcccacc ccctctcccc gctggcccat
     3841 taccgccctt gggcggtcgg gacgagagcg cagcgagcgg gccgggtggg tggctgtttg
     3901 gtgcttgcat ggagatatgc tattatggta tttgcgcggt ggtttggcag ggagcaaggt
     3961 gtaacctgcg cttcgcttgc ttctcaccct ccggccttcg gcctacgccc ccgctccctt
     4021 cggtcggggg gttgctgcgc gtttcgcttc gcttcacttg ctggagggct tcgcccatga
     4081 acactgacga atccaagcgc cgccgccgtg ggcctgcgcc gctcgacgcc acggacaagc
     4141 gcgggcatac cgtttctgtg cgcctcaacg atgccgagct tgcgcggctt gattcgcagc
     4201 gtgatgcagt gcagatgcaa cgcggtgagt acctacgcgc cgccgccctt catcgcctgc
     4261 cgcccaccat cccggaagtg aaccgggaag cgtgggccgc gttggcccgc acggcggcca
     4321 acctgaacca gatcgcgcac cggctgaacg ctggcgacgc gctcccgctg gccgaggtgc
     4381 gggctacgct cgacgccttc aggcgtgacc tgataggcgt caagcacgac gcagaggacg
     4441 cagaagatga aagcgaaagt taacaggggt ggcggttttc gcggtgcgct gaactacgtt
     4501 ttcgacgtgg gcaaggaagc cacgcacacg aaaaacgcgg agcgagtcgg cggcaacatg
     4561 gccgggaatg acccccgcga actgtcgcgg gagttctcag cagtgcgcca gttgcgcccg
     4621 gacatcggca agcccgtctg gcattgctcg ctgtcactgc ctccttgcga gcgcctgagc
     4681 gccgagaagt gggaagccgt ggcggctgac ttcatgcagc atatggggtt tgaccagaac
     4741 aatacgccgt gggtggccgt gcgccaccaa gacacggaca aggatcacat acacatcgtc
     4801 gccagccggg tagggctgga cgggaaagtg tggctgggcc agtgggaagc ccgccgcgcc
     4861 atcgaagcga cccaagagct tgagcatacc cacggcctga ccttgacgcc ggggctgggc
     4921 gatgcgcggg ccgagcgccg gaagctgacc gccgaggaga tcaacatggc cgtgagaacg
     4981 ggcgacgaac cgccgcgcca gcgtctgcaa cggctgctgg atgaggcggt gaaggacaag
     5041 ccgaccgcgc cagaactggc cgagcgacta caggccgcag gcgtaggcgt ccgggcaaac
     5101 ctcgccagca cagggcgcat gaacggcttt tcctttgaag tggccggggt ggcgttcaag
     5161 ggcagcgact tgggcaaggg ctacacatgg gcggggctac agaaagcagg ggtgacttat
     5221 gacgaagcta gagaccgtgc gggccttgaa cgattccggc ccacagttgc agatcgtgga
     5281 gagcgtcagg acgttgcagc agtccgtgag cctgatgcac gaggacttga agcgcctacc
     5341 gggcgcagtc tcgaccgaga cggtgcagac cttggagccg ctggcccgac tccggcagga
     5401 tgtgacgcag gtactggaag cctacgacaa ggtgacagcc atccagcgca agacgctgga
     5461 cgagctgacg cagcagatga gcgcgagcgc ggcgcaggcg ttcgagcaga aggccgggaa
     5521 gctggacgcg accatctccg acctgtcgcg cagcctgtca gggctgaaaa cgagcctcag
     5581 cagcatggag cagaccgcgc agcaagtggc gagcctgccg ggcaagctgg cgagcgcaca
     5641 gcagggcatg accaaggccg ccgaccaact gaccgaggca gcgaacgaga cgcgcccgc
//
