LOCUS       NZ_CP109777             8401 bp    DNA     circular CON 16-APR-2024
DEFINITION  Shigella sonnei strain CIP_106347 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP109777
VERSION     NZ_CP109777.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31285702
            Assembly: GCF_025908455.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   De Silva,P.M., Bennett,R.J., Kuhn,L., Ngondo,P., Debande,L.,
            Njamkepo,E., Ho,B., Weill,F.-X., Marteyn,B.S., Jenkins,C. and
            Baker,K.S.
  TITLE     Escherichia coli killing by epidemiologically successful sublineages
            of Shigella sonnei is mediated by colicins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   De Silva,P.M., Bennett,R.J., Kuhn,L., Ngondo,P., Debande,L.,
            Njamkepo,E., Ho,B., Weill,F.-X., Marteyn,B.S., Jenkins,C. and
            Baker,K.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) Department of Clinical Infection,
            Microbiology and Immunology, University of Liverpool, Crown Street,
            Liverpool L69 7ZB, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: UniCycler v. 0.4.8
            Assembly Name                     :: CIP_106347
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025908455.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:58:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,023
            Genes (coding)                    :: 4,319
            CDSs (with protein)               :: 4,319
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 704
            CDSs (without protein)            :: 704
            Pseudo Genes (ambiguous residues) :: 0 of 704
            Pseudo Genes (frameshifted)       :: 193 of 704
            Pseudo Genes (incomplete)         :: 510 of 704
            Pseudo Genes (internal stop)      :: 123 of 704
            Pseudo Genes (multiple problems)  :: 114 of 704
            Pseudo Genes (short protein)      :: 3 of 704
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109777.1.
            Bacteria and source DNA available from CNRSS 99-9901, Institut
            Pasteur.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="CIP_106347"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="unnamed1"
                     /geo_loc_name="France"
                     /lat_lon="48.8566 N 2.3522 E"
                     /collection_date="1999"
                     /collected_by="Institut Pasteur"
                     /genotype="3.7"
     ARG             4..344
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="41.7"
     ARG             7931..8400
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="57.93"
     gene            405..1208
                     /gene="aph(3'')-Ib"
                     /locus_tag="OHN14_RS25580"
                     /old_locus_tag="OHN14_25580"
     ARG             406..1205
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1208..2044
                     /locus_tag="OHN14_RS25585"
                     /old_locus_tag="OHN14_25585"
     CDS             1208..2044
                     /locus_tag="OHN14_RS25585"
                     /old_locus_tag="OHN14_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            2350..2592
                     /locus_tag="OHN14_RS25590"
                     /old_locus_tag="OHN14_25590"
     CDS             2350..2592
                     /locus_tag="OHN14_RS25590"
                     /old_locus_tag="OHN14_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(2624..3274)
                     /gene="tetR(A)"
                     /locus_tag="OHN14_RS25595"
                     /old_locus_tag="OHN14_25595"
     CDS             complement(2624..3274)
                     /gene="tetR(A)"
                     /locus_tag="OHN14_RS25595"
                     /old_locus_tag="OHN14_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            3380..4579
                     /gene="tet(A)"
                     /locus_tag="OHN14_RS25600"
                     /old_locus_tag="OHN14_25600"
     ARG             3381..4576
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<4546..4734)
                     /locus_tag="OHN14_RS25605"
                     /old_locus_tag="OHN14_25605"
                     /pseudo=""
     CDS             complement(<4546..4734)
                     /locus_tag="OHN14_RS25605"
                     /old_locus_tag="OHN14_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4778..5200)
                     /locus_tag="OHN14_RS25610"
                     /old_locus_tag="OHN14_25610"
     CDS             complement(4778..5200)
                     /locus_tag="OHN14_RS25610"
                     /old_locus_tag="OHN14_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            5385..5825
                     /locus_tag="OHN14_RS25615"
                     /old_locus_tag="OHN14_25615"
     CDS             5385..5825
                     /locus_tag="OHN14_RS25615"
                     /old_locus_tag="OHN14_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(6592..6741)
                     /locus_tag="OHN14_RS25620"
                     /old_locus_tag="OHN14_25620"
     CDS             complement(6592..6741)
                     /locus_tag="OHN14_RS25620"
                     /old_locus_tag="OHN14_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            7312..7470
                     /locus_tag="OHN14_RS25845"
     CDS             7312..7470
                     /locus_tag="OHN14_RS25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             1209..2041
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6777..6950)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          6874..7479
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg
       61 ggttttttct gggggctgct cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat
      121 tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg
      181 cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac actcgctgca gagcttgccg
      241 ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg cgcgacgggc
      301 tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgcac attcgggata
      361 tttctctata ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat cgaactaata
      421 ttttttttgg tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt
      481 ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg
      541 gtgagctcgc tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc
      601 ccgaggtcat caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc
      661 cgggagtacc ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg
      721 ggcagcaact tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc
      781 tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact
      841 tcttaccgga cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag
      901 agctaccggt gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct
      961 gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg
     1021 ggcggctcgg aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag
     1081 agaactgggc agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg
     1141 ggatcgaagc ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt
     1201 ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct
     1261 cattgcggac accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc
     1321 aatcgtcaag ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta
     1381 tctggtatgg cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat
     1441 gttgctcgaa tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta
     1501 ccaggcgacc gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc
     1561 cctgccttct gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg
     1621 cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca
     1681 aatgatgagc aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat
     1741 catgttctcc agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg
     1801 ctttggcgcc gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc
     1861 tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg
     1921 cctgctcgac caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga
     1981 agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata
     2041 ctagatatca agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc
     2101 gctggccaaa gccttagcgt aggattccgc cccttcccgc aaacgacccc tggaagcccc
     2161 gtagcgacgc ggagaggggc gagacaagcc aagggcgcag gctcgatgcg cagcacgaca
     2221 tagccggttc tcgcaaggac gagaatttcc ctgcggtgcc cctcaagtgt caatgaaagt
     2281 ttccaacgcg agccattcgc gagagccttg agtccacgct agatctatct catctgcgca
     2341 aggcagaacg tgaagacggc cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc
     2401 ggcgcgcgga cccgcgccac ggcccacgag cggacgccgc agcaggagcg ccagaaggcc
     2461 gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc
     2521 gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg
     2581 gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc
     2641 tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg
     2701 cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc
     2761 gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt
     2821 cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat
     2881 tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg
     2941 tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg
     3001 cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt
     3061 cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga
     3121 gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt
     3181 ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg
     3241 cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca
     3301 taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag
     3361 gctggtccgg aggccagacg tgaaacccaa cagacccctg atcgtaattc tgagcactgt
     3421 cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga
     3481 tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt
     3541 gatgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc
     3601 ggtcttgctc gtctcgctgg ccggcgctgc tgtcgactac gccatcatgg cgacggcgcc
     3661 tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc
     3721 ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg
     3781 cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat
     3841 gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt
     3901 cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg
     3961 ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc
     4021 cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt
     4081 cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc
     4141 atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct
     4201 cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct
     4261 tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg
     4321 catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg
     4381 gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct
     4441 cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg
     4501 cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga gaaattcttc
     4561 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4621 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4681 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4741 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4801 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4861 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4921 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4981 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     5041 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     5101 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     5161 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     5221 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     5281 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     5341 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     5401 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5461 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5521 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5581 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5641 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5701 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5761 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5821 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5881 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5941 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     6001 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     6061 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     6121 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     6181 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgtata
     6241 agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt caggaagaat
     6301 aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc acgatctccc
     6361 cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa atggtcatac
     6421 acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa aaaggaatat
     6481 ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat gaaaataaag
     6541 aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa gctataacgc
     6601 ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt cagtaacctg
     6661 cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg aatccagttg
     6721 ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt tagcgggttt
     6781 cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg cttactatgt
     6841 tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa ggcagcaccg
     6901 gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac tgactcgcta
     6961 cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg gaaatttcct
     7021 ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa gccgtttttc
     7081 cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca gtggtggcga
     7141 aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct
     7201 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt
     7261 ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa
     7321 ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg
     7381 gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat
     7441 agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt
     7501 aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc
     7561 gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta cgattcattg
     7621 aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac
     7681 cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt
     7741 gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat
     7801 ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct
     7861 ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat tattattata
     7921 gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt
     7981 ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg
     8041 atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg
     8101 cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca
     8161 gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg
     8221 cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa
     8281 ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat
     8341 cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc
     8401 a
//
