LOCUS       NZ_CP109610             3991 bp    DNA     circular CON 20-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-231 plasmid pKP18-231-3, complete
            sequence.
ACCESSION   NZ_CP109610
VERSION     NZ_CP109610.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31269456
            Assembly: GCF_026651915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Qin,S. and Zhang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2022) School of Pharmacy, Zhengzhou University,
            100 Science Avenue, Zhengzhou 450052, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC NGS Cell v. CLC Genomics Workbench 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/20/2024 05:21:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,656
            Genes (coding)                    :: 5,551
            CDSs (with protein)               :: 5,551
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 69 of 105
            Pseudo Genes (internal stop)      :: 15 of 105
            Pseudo Genes (multiple problems)  :: 12 of 105
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP109610.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-231"
                     /isolation_source="clinical"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-231-3"
                     /geo_loc_name="China:Henan Province"
                     /collection_date="2018-09-17"
     gene            join(3760..3991,1..743)
                     /locus_tag="OGW23_RS28830"
                     /old_locus_tag="OGW23_28835"
     CDS             join(3760..3991,1..743)
                     /locus_tag="OGW23_RS28830"
                     /old_locus_tag="OGW23_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(970..1082)
                     /locus_tag="OGW23_RS28835"
                     /old_locus_tag="OGW23_28840"
                     /pseudo=""
     CDS             complement(970..1082)
                     /locus_tag="OGW23_RS28835"
                     /old_locus_tag="OGW23_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1490..1681
                     /locus_tag="OGW23_RS28840"
                     /old_locus_tag="OGW23_28845"
     CDS             1490..1681
                     /locus_tag="OGW23_RS28840"
                     /old_locus_tag="OGW23_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1681..2103
                     /locus_tag="OGW23_RS28845"
                     /old_locus_tag="OGW23_28850"
     CDS             1681..2103
                     /locus_tag="OGW23_RS28845"
                     /old_locus_tag="OGW23_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2907..3179
                     /locus_tag="OGW23_RS28850"
                     /old_locus_tag="OGW23_28855"
     CDS             2907..3179
                     /locus_tag="OGW23_RS28850"
                     /old_locus_tag="OGW23_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          complement(971..1442)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 atggtggtgg aactaagttc atagaacaaa tattgcttct tatttctgtt tcggggatct
       61 ctgcgtatct tgggtacgca ttactggatg gtcttgccaa caaagtatta aaagagcaag
      121 tagacggtat agataaaaag caacaagatt tggaggctga acaagatgag tttaaagatg
      181 agcttgatag gagcaaggag ctgatagaac agcttgaaat ggataagaaa acaacaaaat
      241 tcgagctagg atactttaaa gccatttcag ctgtggataa agcagaatca atgatgtcca
      301 ttccggatga ggctctttct gttaagaaaa aactaactga ggcattagat gctgtaacgg
      361 aatcattgtc tcttgttaaa agagaggatg tagctaagga tgactacgac aagctcttag
      421 tgcttaaagc ttatattttg aaaagacttg atcgtattga tgatgctctg tcaattacgg
      481 atgagttact tatgagtaat gaggataacc ctatacttat ttacaataag gcttgctatc
      541 aatatatttt gcgtcgttgc caagctgata actccgatat taaggatatg attcgcaggg
      601 ctctgactat taaagttaca gatcctgaat tcattcgcag gcaagaaaaa ataagaacca
      661 aggtcatagg aaataaagat aatgatcttg aagggttgtt tactgacgct gagctggagg
      721 aattaaaggt ggctatcaaa taggcagtgg ctagctctat gaattccaaa aagatccttg
      781 acgatccttt gaggtgcagc gtactctacc cctcaaacga aaaaaccacc cggcaaggtg
      841 gtttttcgaa ggttcagtaa ccggcaagtt tctgaaccga ggtaactggc ttagaggagc
      901 gcagtcacca aaacagttcg tttagtttag ccttaactgg cgcataactt caagactaac
      961 tcctctaaat caattaccag tggctgctgc caatggcgat aagtcgtgtc tttccgggtt
     1021 ggactcaaga gatagttact ggaacgggcg cagcagtcgg actgaacggg gggttcgtgc
     1081 atacagtcca gcttggagcg aactgccttc ccggagccga gtatcaggca tggaatgaga
     1141 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     1201 acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc
     1261 actgatttga gcgtccgttt ccgcgatgct tgtcaggggg gcggagccta tggaaaaaca
     1321 gccgttcggc ggcctttgct tttctgcctc atatcttggt tgcctacgtt tttatgtaca
     1381 cgctcctgcc gctcgccgca gccgaacggc tggagcgtag cgactgagtg agcgaggaag
     1441 cggaaaagag actagtttga cattgagcac tgacgctctg aggcttctta tgacaaaaca
     1501 acagaaaaca gcgctgaaca tggctaaatt catccagaat cagtctctgc tcctgctgga
     1561 taagcttaac gagcttgatc tcgatgtcga ggccgacctt tgtgagaaac tccatgatga
     1621 tgcggagcat cttttccgta cactgtcctc gagactggat gaattacagg atggtaactg
     1681 atggccatag tgtctattag tgaagcggca cggcttgtta aggttagtcg ccccacaatt
     1741 tacaagatga ttaacagtgg gaagttaagt tacacctctg tggtaaagca cgggaaagct
     1801 attaaagtta ttgatacttc agagctaatt agagtgtttg gttctcttga tggtgtaatc
     1861 gatactgtaa aatatgatgt aaaaagtgac gcagaatcta caggtattaa cagcgttggt
     1921 ttacatgatt tacaacatag aatcgcttta cttgaagctg aaaacgatgg tcttaaaggg
     1981 gctgttaagg ctcgagatga gcatattgat tcccttcgtc aggctatgca gcttctggag
     2041 cacaagcacg aaccttcttc accaccccat tccccgtggt ggaaattctg gaaaaaatcc
     2101 tgatcccgcc atgcttccat tttgctctcc ggccattccg gggagttcct gaaccttccc
     2161 aaaaatcgtc agatccctga acgctcctca ccactaaaaa tacccacaca ctacgcaaaa
     2221 ttaaaatttt gcagatagac ctttattttc aatcagttat ctattttaaa ttacgcaatt
     2281 ttattttttg taaggtgaat ccgagtcgtg gacgactcta tggcgaatcg ccgatgactt
     2341 gcccacgatt catatgtgaa tcacgccata taatcagtac gttatgatga cttgactcta
     2401 gagtcgactt gccgggttgg tggctttttg accatggcga ctctagagtc aagtcacgag
     2461 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataat gtgattgctg
     2521 gtctcgacaa acccgattcc tgaacgtgct gaaaaccggt tttccggctg cgccggcaac
     2581 gcttcacggc cacgcaataa attgcgcacc cgttcaccgt tttcagtctg gtttggtgcg
     2641 ggttttgagt tgcctggcct gaattcaggc tgaaatcccc agacagtcag tcaagtgcat
     2701 aacgattcat gcccgtaaaa cgccctggag cgattttgag gcgttttcag ggggtaagac
     2761 gtaggattta atgggttacg ccccagaatc gttctgaggc cgttttagcg gtgcgtgtaa
     2821 tgggacgtta tggtaaatca acggcggatg cctgagtctg tgttagagtc agcggattgt
     2881 gataatcagt gactcaggga gatgttttgg ctactgacgc tcaggtaaaa gaaattaatg
     2941 cactcatcaa aaaatacccg gactcatgct ccatctgtca tgaagtctat gatgaggatg
     3001 atgtgaccta caccgttttt gggtatgacc ggaaaggtaa aatacaggtc accacgggat
     3061 gctgtgccgg aatgctgaca gagccggttc tgctgggtgt ctgtgggtgc tttgacccgg
     3121 aagaacgtga cgagatcatg cagaatcacc ccatggcaaa gcagtttttc acagagtgat
     3181 tccgtatctg ttgagtctga agcccgcctt gtgcgggctt tttcatatct gcgtgaaatg
     3241 aggcgttatg gtaaatgcac gcattcattc ttttgaaaaa tccagaatga cgaatacatg
     3301 gggcatgttt atatgactat tgagaggaag gatgttattg taaaaaaatc ctttaaattc
     3361 aatcatttaa ttatggtagc caaaggggcg gggcttcgct tattcctaag gaataagcga
     3421 agccccgccc ctttggctac cagtgcatta tacttttttt ttcacccaat gtcaatacat
     3481 gggttgattt tatcatttta agcatttttg ttgttgtgag tgctgggttt atgccctata
     3541 ttcagcgcta agcaagcatg ggcttgcaaa ggaacaatta atgagaggtg ctctatgaaa
     3601 aaaatcaaac ttcacgatga gcgtccagac ttgtaagtgt caagtcagca gaaaaaggca
     3661 gccaaggctg ccttttttgt tattggttag tatatgattg agcttatctt aacttacctg
     3721 aacaaagttt tattgtttgc tttgcgcaag gattctctca tggctttttt taacctattg
     3781 tttgttgcat ctctaatttg ttttggtggt gttatgggga gctattccag aggttgccga
     3841 gactcccaaa ataaattttc aaaagataag gatgagaata ataaaagagc tagtgtttat
     3901 agatttggta tagcatccgc atttatttgt gttcctttca tatcatcatt tcttcatgtt
     3961 gattactcaa gtataatatt tcctgtggct g
//
