LOCUS       NZ_CP107726             3362 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain 2021CK-01361 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP107726
VERSION     NZ_CP107726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21924052
            Assembly: GCF_025853875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     2021CK-01361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,345
            CDSs (total)                      :: 5,230
            Genes (coding)                    :: 4,924
            CDSs (with protein)               :: 4,924
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 68 of 306
            Pseudo Genes (incomplete)         :: 231 of 306
            Pseudo Genes (internal stop)      :: 47 of 306
            Pseudo Genes (multiple problems)  :: 39 of 306
            Pseudo Genes (short protein)      :: 3 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-01361"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2021-08-16"
     gene            complement(415..738)
                     /locus_tag="N4T39_RS26575"
                     /old_locus_tag="N4T39_26575"
     CDS             complement(415..738)
                     /locus_tag="N4T39_RS26575"
                     /old_locus_tag="N4T39_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_172843362.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1379..1537
                     /locus_tag="N4T39_RS26730"
     CDS             1379..1537
                     /locus_tag="N4T39_RS26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_069678388.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKTLLAAAISNWRYTRGKL
                     RGLEVWA"
                     /name="replication initiation protein"
     gene            1823..2254
                     /locus_tag="N4T39_RS26580"
                     /old_locus_tag="N4T39_26580"
     CDS             1823..2254
                     /locus_tag="N4T39_RS26580"
                     /old_locus_tag="N4T39_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_001025244.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEHLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(2395..2610)
                     /locus_tag="N4T39_RS26585"
                     /old_locus_tag="N4T39_26585"
     CDS             complement(2395..2610)
                     /locus_tag="N4T39_RS26585"
                     /old_locus_tag="N4T39_26585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_045350912.1"
                     /translation="MTELEKQLLGALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTVLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2614..3099)
                     /locus_tag="N4T39_RS26590"
                     /old_locus_tag="N4T39_26590"
     CDS             complement(2614..3099)
                     /locus_tag="N4T39_RS26590"
                     /old_locus_tag="N4T39_26590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045350913.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAAILWWQSQQIL
                     DNYMTIREQKSTQAMLSERNSGVQLSTCGDQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(840..1026)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1006..1491
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.816"
                     /coverage="93.0"
                     /evalue="0.0"
     Typing          complement(4..446)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.27"
                     /coverage="94.0"
                     /evalue="1.58e-90"
     Typing          complement(2292..3361)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.756"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgccgttca ctatggtctg ccaggcatcg atgcgcggac gatcggcccg gtcgtaatac
       61 ggctggagac gcctgccggt cagcagctcc gtgttaggaa tcaggaagtt cagctccagc
      121 cgccccttgt cccggtgctc aacccacagc acgctgtact ggtctttatc gagtccgggc
      181 atcagaaccc gctcaaagct cgccatcagc ttttcccgct ggccgggcgg taagtccttt
      241 tcggcaaagg acaggacgcc ggatgtgtac tttttggcgt agggcgaggc gtcgataagc
      301 tcccggactt cctccggctt cccctgcaga acgctggcac cgtcgcgctg gcggtctttg
      361 cccagcaaat aatcccccgg accggcgccc ccgccgcgcc cccgggggtg aaacttaacg
      421 atcatcgtct gccccctttt ccagcacggc gtgccgcagc cgctcgagtc cggcatcgat
      481 ggccatcagc gcggcgacga cctgtacacg gtcgtgcccg ctgccgccac ctccgttaac
      541 ctgccgggca atctggttaa ggttgttccc catgccggca agctgacgaa gcagcgccgg
      601 cgagagcgaa ggaagtttac cggcgcgggc tggcttctcg tccaggcacg tctgccgcat
      661 ccaggctgcg agctgtttac cgtcgcagcg ctccagcaga cgccggtgct cgtcttccgt
      721 tacccacatc gtgagcattt tgctgcgttt gtctgccaat ccagcctcct gataacgccg
      781 ggacgggcgg gaaatctttt cccggtcggc gcggtgttgg gcaagccgca ggcgcgacag
      841 tggttttggc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta gcggagtgta
      901 tactggctta atatgttaaa tcgaagtgga aattcaggaa agtgcttcat gtggcagggg
      961 aaaaatgcgg ctgtgggacg taagtgcgaa atactggaga cgatgaaatg acgcctcctc
     1021 gctcactcgg tcgctacgct cctgccgtga gactgcggcg ggcgttacct gctcacgaat
     1081 agcgagataa agaggctgag cggaaatcgg gaagggctgg taaaggatgc ggcgttgccg
     1141 tttttccata ggctccgccc ccctgacaag catcacaaaa tctgacgctc aaatcagtgg
     1201 cggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag ctccctcgtg
     1261 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     1321 tctcattcca cgcctgacac tcagttccgg gaaggcagtt cgctccaagc tggactgtat
     1381 gcacgaaccc cccgttcagt ccgactgctg cgccttgtcc ggtaactatc gtcttgagtc
     1441 caacccggaa agacacgaaa aaacgctact ggcagcagcc attagtaact ggagatatac
     1501 gagaggcaaa ttgagaggtc ttgaagtatg ggcctgagag aggctacact gaaagaacag
     1561 ttttggtttc tgcggtctcg tatacccagt tacctggtta agcagttccc caactgacct
     1621 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt ttcagagcaa gagattacgc
     1681 tcagccataa atgatctcaa gaagatccac tccaacaact ccaaatctgc aagacacaaa
     1741 ttgttacact tactaattag taaattaact tgttttgttg ttttatgaat aaataaatca
     1801 atttcacaca gcagagaaca atatgaacat ccctactcat ttgaaacaca aaccgattat
     1861 tgctgtagaa aactatgatc gaattgacgg cccctacgct gacgacactg atgccatggg
     1921 gctttctgtt ggaattgctc aatggaacac tcccggatat acagagctat cagcaaaagt
     1981 ctggcgcaac actggagaaa agtggtcacg gcaatctgaa gagctgcctc ttcaccgtgt
     2041 catcgatttg gcaacgctta tatgcatagc aatggattac agtaagcatg gtcgcttatc
     2101 ttccagcgac gacttcccag tcagtcgttc aggaggtaat cctgagttat ctcgccacat
     2161 tgatttgatg aagaacgaac taaaagagaa tcacgagcat ttagatgaaa gccttaaacg
     2221 tttagcagca gaactaaaac gtttaggtta ttaagtagca agccgtccat ctgggcggct
     2281 tttcttatta aagcgttggg ccgttataac ttcgctgccg agttgaacgc tcaagttcta
     2341 gcgctttttg gtgccgagcc gcgcgctcct gctcccggtg cgcctcgata ccattcagcc
     2401 ttgtgacaac cggcgcagct gatcgctcaa actctgcacc tgcctgctca agaccgtgac
     2461 gcgctcgctc agcgccgcgt tctcccgttg cataagacca gacatcgtcc gccattccgc
     2521 gaaggcgttc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct gctctaatgc
     2581 gcccagcaac tgtttttcca gctccgtcat gtgctatttc cccgccagta tcatccagct
     2641 cgaatcctct ccgaaccgtc ccgcttccgg gttcaccctc acgcagcggc gtctctgatc
     2701 gccgcaggtc gagagctgca cgccgctgtt ccgctcggac agcatggcct gcgtgctctt
     2761 ctgctcccgg atggtcatgt aattgtcgag tatctgctga ctctgccacc acagaatggc
     2821 cgcgctcgaa gcgatcagca gcgcggagac caggacgatg gtcagccacg tctggctgac
     2881 catacgcagc atccctttcg tgcgctggct catggctgag gacagcttcc ggtcgtggtc
     2941 gaggatggcg gcgctgattc tcttctcgct ttcgctcagt tccgctctga cagactgctc
     3001 gtgctcgctg aatgcggctt tcagcatctc gccggtactc tgctgctgcg ctttcgattt
     3061 ctgctctaag tccttcgcca gcgttaaaag actgttcata gatggctccc ttcagtcgga
     3121 tgtttcgccc cccgtccggg tcggcaatgc tgatactgct tttcgtggtg cgcaccacct
     3181 caaaacccgc gctttccagc gcctcagtga cgtcctgacg ggttttcagc tccccggatg
     3241 aggcaagggc aagcaggccg cgcgtaatag cctgggcggc ctcctgcttc gcttccggca
     3301 gcgcggacgg cgtgaccagc gcccgccggt tttccggcgc gttcgggtcg tgcagcccca
     3361 gc
//
