LOCUS       NZ_CP107724            17056 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain 2021CK-01361 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP107724
VERSION     NZ_CP107724.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21924052
            Assembly: GCF_025853875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17056)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     2021CK-01361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17056)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:25:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,345
            CDSs (total)                      :: 5,230
            Genes (coding)                    :: 4,924
            CDSs (with protein)               :: 4,924
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 306
            CDSs (without protein)            :: 306
            Pseudo Genes (ambiguous residues) :: 0 of 306
            Pseudo Genes (frameshifted)       :: 68 of 306
            Pseudo Genes (incomplete)         :: 231 of 306
            Pseudo Genes (internal stop)      :: 47 of 306
            Pseudo Genes (multiple problems)  :: 39 of 306
            Pseudo Genes (short protein)      :: 3 of 306
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107724.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-01361"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2021-08-16"
     gene            46..1725
                     /locus_tag="N4T39_RS26455"
                     /old_locus_tag="N4T39_26455"
     CDS             46..1725
                     /locus_tag="N4T39_RS26455"
                     /old_locus_tag="N4T39_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="WP_000179844.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEAA
                     DMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQK
                     RFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDL
                     QGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVVT
                     LEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEALRRGC
                     EQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSENKAALT
                     LRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLVDFLPILR
                     RTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPEGQHYLEIP
                     YRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQKATRKARRDA
                     DRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            1728..2627
                     /locus_tag="N4T39_RS26460"
                     /old_locus_tag="N4T39_26460"
     CDS             1728..2627
                     /locus_tag="N4T39_RS26460"
                     /old_locus_tag="N4T39_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011899345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="WP_011899345.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVEA
                     LNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQMP
                     SEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAGNSV
                     NRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDDCCSL
                     LASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEAINHRT
                     LSISTTSRATKTSRSDCK"
                     /name="TniB family NTP-binding protein"
     gene            complement(2684..3184)
                     /locus_tag="N4T39_RS26465"
                     /old_locus_tag="N4T39_26465"
     CDS             complement(2684..3184)
                     /locus_tag="N4T39_RS26465"
                     /old_locus_tag="N4T39_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000376623.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARID
                     RTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHAGR
                     GVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAERLE
                     LEI"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(3312..4151)
                     /gene="sul1"
                     /locus_tag="N4T39_RS26470"
                     /old_locus_tag="N4T39_26470"
     ARG             complement(3316..4151)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4145..4492)
                     /locus_tag="N4T39_RS26475"
                     /old_locus_tag="N4T39_26475"
     CDS             complement(4145..4492)
                     /locus_tag="N4T39_RS26475"
                     /old_locus_tag="N4T39_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WP_000679427.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            complement(4661..5293)
                     /locus_tag="N4T39_RS26480"
                     /old_locus_tag="N4T39_26480"
     CDS             complement(4661..5293)
                     /locus_tag="N4T39_RS26480"
                     /old_locus_tag="N4T39_26480"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B-2 chloramphenicol O-acetyltransferase
                     CatB2"
                     /protein_id="WP_012477387.1"
                     /translation="MTNYFESPFKGKLLTEQVKNPNIKVGRYSYYSGYYHGHSFDDCAR
                     YLLPDRDDVDQLIIGSFCSIGSGAAFIMAGNQGHRYDWVSSFPFFYMNEEPAFAKSVDA
                     FQRAGDTVIGSDVWIGSEAMIMPGIKIGHGAVIGSRALVAKDVEPYTIVGGNPAKSIRK
                     RFSEEEISMLLDMAWWDWPLEQIKEAMPFLCSSGIASLYRRWQGTSA"
                     /name="type B-2 chloramphenicol O-acetyltransferase CatB2"
     gene            complement(5346..6137)
                     /gene="aadA1"
                     /locus_tag="N4T39_RS26485"
                     /old_locus_tag="N4T39_26485"
     ARG             complement(5350..6137)
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6254..7048)
                     /gene="aph(3')-XV"
                     /locus_tag="N4T39_RS26490"
                     /old_locus_tag="N4T39_26490"
     ARG             complement(6258..7048)
                     /name="aph(3')-XV"
                     /gene="aph(3')-XV"
                     /gene_name="aminoglycoside O-phosphotransferase APH(3')-XV"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7119..7673)
                     /locus_tag="N4T39_RS26495"
                     /old_locus_tag="N4T39_26495"
     CDS             complement(7119..7673)
                     /locus_tag="N4T39_RS26495"
                     /old_locus_tag="N4T39_26495"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ib4"
                     /protein_id="WP_003159191.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQSLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     DGPAVYMVQTRQAFERTRSDA"
                     /name="aminoglycoside N-acetyltransferase AAC(6')-Ib4"
     gene            complement(7781..8581)
                     /locus_tag="N4T39_RS26500"
                     /old_locus_tag="N4T39_26500"
     CDS             complement(7781..8581)
                     /locus_tag="N4T39_RS26500"
                     /old_locus_tag="N4T39_26500"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase VIM-1"
                     /protein_id="WP_013263789.1"
                     /translation="MLKVISSLLVYMTASVMAVASPLAHSGEPSGEYPTVNEIPVGEVR
                     LYQIADGVWSHIATQSFDGAVYPSNGLIVRDGDELLLIDTAWGAKNTAALLAEIEKQIG
                     LPVTRAVSTHFHDDRVGGVDVLRAAGVATYASPSTRRLAEAEGNEIPTHSLEGLSSSGD
                     AVRFGPVELFYPGAAHSTDNLVVYVPSANVLYGGCAVHELSSTSAGNVADADLAEWPTS
                     VERIQKHYPEAEVVIPGHGLPGGLDLLQHTANVVKAHKNRSVAE"
                     /name="subclass B1 metallo-beta-lactamase VIM-1"
     gene            8748..9761
                     /gene="intI1"
                     /locus_tag="N4T39_RS26505"
                     /old_locus_tag="N4T39_26505"
     CDS             8748..9761
                     /gene="intI1"
                     /locus_tag="N4T39_RS26505"
                     /old_locus_tag="N4T39_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_000845054.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            complement(10175..10711)
                     /locus_tag="N4T39_RS26510"
                     /old_locus_tag="N4T39_26510"
     CDS             complement(10175..10711)
                     /locus_tag="N4T39_RS26510"
                     /old_locus_tag="N4T39_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003159187.1"
                     /translation="MNEFRRLAAKIDQHMQQLAAQGVSEAHAIINRMMGYGPDLHRIWV
                     GTSDQQLMALSREFPGFYRYARIMEEASEAERRKASRPYDGMAEFSEQHKQMGAQLLTT
                     AATLERGYQAFRASGSLQDFRPQLDELGRLHRQWLSDLEAFKDSLRTQGAEPKVLEYVN
                     EAFGRLAERIKQLAG"
                     /name="hypothetical protein"
     gene            complement(10723..11118)
                     /locus_tag="N4T39_RS26515"
                     /old_locus_tag="N4T39_26515"
     CDS             complement(10723..11118)
                     /locus_tag="N4T39_RS26515"
                     /old_locus_tag="N4T39_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017172645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_003159186.1"
                     /translation="MKVAVDTNVLVRAVVRDDPAQADVAAAVLTDAELIAVALPCLCEF
                     VWVLLRVYGFQQADAASAIRALLAAANVEVNRPAVEAGLLVLDAGGDFADGVIAYEGNW
                     LGGETFVSFDKKAVALLTAQGQSTRLL"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(11115..11366)
                     /locus_tag="N4T39_RS26520"
                     /old_locus_tag="N4T39_26520"
     CDS             complement(11115..11366)
                     /locus_tag="N4T39_RS26520"
                     /old_locus_tag="N4T39_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_003108276.1"
                     /translation="MTTLTVTARGQVTFRKDVLQHLGIRPGDKIELDLLPDGRGVLKAA
                     RPAGTIASFVGLLAGKTQKVATIEEINEAAAQGWAGKQ"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            11548..12087
                     /locus_tag="N4T39_RS26525"
                     /old_locus_tag="N4T39_26525"
     CDS             11548..12087
                     /locus_tag="N4T39_RS26525"
                     /old_locus_tag="N4T39_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052932.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_058689425.1"
                     /translation="MALIGYARVSTAEQDTALQTDALRKAGCERVFEDTASGAKADRPG
                     LADALAYLRDGDVLAVWRLDRLGRSMPHLIETIGALEARGVGFRSLTEAIDTTTPGGRL
                     IFHVFGALGQFERDLIRERTKAGLTAAAARGRKGGRKPVVTADKLQRAREHIANGLNVR
                     EAATRLKVSKTALLQS"
                     /name="recombinase family protein"
     gene            complement(12121..12814)
                     /locus_tag="N4T39_RS26530"
                     /old_locus_tag="N4T39_26530"
                     /pseudo=""
     CDS             complement(12121..12814)
                     /locus_tag="N4T39_RS26530"
                     /old_locus_tag="N4T39_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            12951..13811
                     /locus_tag="N4T39_RS26535"
                     /old_locus_tag="N4T39_26535"
     CDS             12951..13811
                     /locus_tag="N4T39_RS26535"
                     /old_locus_tag="N4T39_26535"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase SHV-12"
                     /protein_id="WP_002904004.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIEM
                     DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV
                     SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWET
                     ELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLP
                     AGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALI
                     EHWQR"
                     /name="extended-spectrum class A beta-lactamase SHV-12"
     gene            complement(13832..14593)
                     /locus_tag="N4T39_RS26540"
                     /old_locus_tag="N4T39_26540"
     CDS             complement(13832..14593)
                     /locus_tag="N4T39_RS26540"
                     /old_locus_tag="N4T39_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226821.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WP_002210513.1"
                     /translation="MIPIERHQRILALVEQRGAVSINELTEILGVSHMTIRRDVSKLEE
                     QGLLVSVSGGVRAVSRLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTT
                     LALARAILDRNDLQVVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRH
                     LAIDTAFISASGWDSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPL
                     TRFTTIITDRHLSDAAASHIARHACEVLRAG"
                     /name="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            complement(14921..16309)
                     /locus_tag="N4T39_RS26545"
                     /old_locus_tag="N4T39_26545"
     CDS             complement(14921..16309)
                     /locus_tag="N4T39_RS26545"
                     /old_locus_tag="N4T39_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_227543520.1"
                     /translation="MGSCAAPSAKGDDKFITTDYLQQCQNGDHYWVSAYVTPIAKNGSI
                     VEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSAMAAG
                     MLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGRTDEF
                     GQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQIATA
                     VNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQVIHE
                     LEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAARTQQ
                     STTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEITDMNA
                     QIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELAKQFW
                     EKRG"
                     /name="methyl-accepting chemotaxis protein"
     gene            16300..17004
                     /locus_tag="N4T39_RS26550"
                     /old_locus_tag="N4T39_26550"
     CDS             16300..17004
                     /locus_tag="N4T39_RS26550"
                     /old_locus_tag="N4T39_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(4149..4492)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7123..7673)
                     /name="aac(6')-Ib4"
                     /gene="aac(6')-Ib4"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(6')-Ib4"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             12952..13808
                     /name="blaSHV-12"
                     /gene="blaSHV-12"
                     /gene_name="extended-spectrum class A beta-lactamase
                     SHV-12"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4665..5293)
                     /name="catB2"
                     /gene="catB2"
                     /gene_name="type B-2 chloramphenicol O-acetyltransferase
                     CatB2"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7785..8581)
                     /name="blaVIM-1"
                     /gene="blaVIM-1"
                     /gene_name="subclass B1 metallo-beta-lactamase VIM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6255..6556)
                     /name="aphA15"
                     /gene="aphA15"
                     /gene_name="APH(3')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="37.88"
     ARG             complement(7120..7745)
                     /name="AAC(6')-Ib8"
                     /gene="AAC(6')-Ib8"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="94.69"
                     /coverage="92.44"
     ARG             complement(5347..6125)
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.23"
                     /coverage="98.48"
ORIGIN
        1 atctcgacta tgctcaatac tcgtgtgcac caaagcgagg tgagcatggc gacggacacc
       61 ccacggattc cagaacaagg cgtggccact ctgcctgatg aggcttggga gcgtgcgcgc
      121 cgtcgtgcgg agatcatcag tccgttggcg cagtcggaga cggtcgggca cgaagcggcc
      181 gatatggcgg ctcaggcgct gggcttgtct cggcgccagg tatacgttct gatccggcgt
      241 gcccggcaag gcagcggcct cgtgacggat ctggtgcccg gccagtccgg tggaggtaaa
      301 ggtaaggggc gcttgccgga accggtcgag cgcgtcatcc acgagctact gcaaaagcgg
      361 ttcctgacca agcagaagcg cagcctagcg gcctttcacc gcgaagtcac tcaggtgtgc
      421 aaggctcaaa aactgcgagt gccggcgcgc aataccgtgg ccttacggat cgctagcctt
      481 gacccgcgca aggtcatccg ccggcgggaa ggccaggatg ccgctcgtga cctacaaggt
      541 gtgggcggcg agcctcctgc cgtgaccgcg ccgctggagc aggtgcagat agaccatacg
      601 gtcatcgacc tgatcgtggt cgatgaccgc gaccgacaac ctattggccg cccgtacctg
      661 accctcgcca tcgacgtgtt cacccgctgc gtgctcggca tggtcgtcac gctggaagcg
      721 ccgtctgccg tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg
      781 ctggaaggac tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac
      841 ctagacaacg cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc
      901 atccggctgg actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc
      961 atcggcacgg cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct
     1021 gaccagcgcg gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag
     1081 cgctggctca cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa
     1141 ccgccggccg cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca
     1201 cgcgctactt cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc
     1261 ggctttgtca tcgaccacat ccactactac gccgatgcgc tcaagccgtg gattgcgcgg
     1321 cgtgaacgct ggccgtcctt tctgatccgg cgcgatccgc gcgacatcag ccgtatctgg
     1381 gtcctggaac cggagggaca gcattacctg gaaattccct accgtacctt gtcgcatccg
     1441 gctgtcaccc tctgggaaca acggcaggcg ctggcgaaac tgcggcagca agggcgcgaa
     1501 caggtggatg agtcggcgct gttccgcatg atcggccaga tgcgtgagat tgtgaccagc
     1561 gcgcagaagg ccacacgcaa ggcgcggcgt gacgcggatc gccgccagca cctcaagaca
     1621 tcagctcggc cggacaagcc cgttccgccg gatacggata ttgccgaccc gcaggcagac
     1681 aacttgccac ccgccaaacc gttcgaccag attgaggagt ggtagccgtg gacgaatatc
     1741 ccatcatcga cctgtcccac ctgctgccgg cggcccaggg cttggcccgt cttccggcgg
     1801 acgagcgcat ccagcgcctt cgcgccgacc gctggatcgg ctatccgcgc gcagtcgagg
     1861 cgctgaaccg gctggaagcc ctttatgcgt ggccaaacaa gcaacgcatg cccaacctgc
     1921 tgctggttgg cccgaccaac aatggcaagt cgatgatcgt cgagaagttc cgccgcaccc
     1981 acccggccag ctccgacgcc gaccaggagc acatcccggt gttggtcgtg cagatgccgt
     2041 ccgagccgtc cgtgatccgc ttctacgtcg cgctgctcgc cgcgatgggc gcgccgctgc
     2101 gcccacgccc acggttgccg gaaatggagc aactggctct ggcactgctg cgcaaggtcg
     2161 gcgtgcgcat gctggtgatc gacgagctgc acaacgtgct ggccggcaac agcgtcaacc
     2221 gccgggaatt cctcaacctg ctgcgcttcc tcggcaacga actgcgcatc ccgttggttg
     2281 gggtaggcac gcgcgacgcc tacctagcca tccgctccga tgaccagttg gaaaatcgct
     2341 tcgagccgat gatgctgccg gtatgggagg ccaacgacga ttgctgctca ctgctggcca
     2401 gcttcgccgc ttcgctcccg ctgcgccggc cttccccaat tgccacgctg gacatggctc
     2461 gctacctgct cacacgcagc gagggcacca taggggaact ggcgcacttg ctgatggcgg
     2521 cggccatcgt cgccgtggag agcggcgagg aagcgatcaa ccatcgcaca ctcagcatat
     2581 cgacaacctc tcgcgcaacc aagacatcgc ggtcggactg caagtgatct tgaagccacg
     2641 ggcccgtccc accccgacat ggacctcgat gcccgaacgg acgttagatt tcgagttcta
     2701 ggcgttctgc gatgaaggtt ggatcccagc cgggattgaa agtgtcgacg tgggtgaatc
     2761 cgagccgctc gtataggcca cgcaggttcg ggtggcagtc gagccgcagc ttggcgcacc
     2821 cctgcgttcg cgcggcatgg cggcaagcct cgatcagcgc ggagctgaca ccccggcccg
     2881 catgtgtccg tcgcaccgcg agcttgtgca gatatgcggc ctcccccttg agggcgtcgg
     2941 gccagaactc gggatcctcg gccgacaagg tgcaacagcc gacgatgccg tcgctgcaac
     3001 tcgcgactag gagctcggat ctcaggacga aggtctccgc gaatgtccgg tcgatccgcg
     3061 cgacgtccca ggcgggcgtt cccttggcgg acatccacgc cgcagcgtcg tgcatcagcc
     3121 gcacaacctc gtcgatatca cccgagcagg cgacccgaac gttcggaggc tcctcgctgt
     3181 ccattcgctc ccctggcgcg gtatgaaccg ccgcctcata gtgcagtttg atcctgacga
     3241 gcccagcatg tctgcgccca ccttcgcgga acctgaccag ggtccgctag cgggcggccg
     3301 gaaggtgaat gctaggcatg atctaaccct cggtctctgg cgtcgcgact gcgaaatttc
     3361 gcgagggttt ccgagaaggt gattgcgctt cgcagatctc caggcgcgtg ggtgcggacg
     3421 tagtcagcgc cattgccgat cgcgtgaagt tccgccgcaa ggctcgctgg acccagatcc
     3481 tttacaggaa ggccaacggt ggcgcccaag aaggatttcc gcgacaccga gaccaatagc
     3541 ggaagcccca acgccgactt cagcttttga aggttcgaca gcacgtgcag cgatgtttcc
     3601 ggtgcggggc tcaagaaaaa tcccatcccc ggatcgagga tgagccggtc ggcagcgacc
     3661 ccgctccgtc gcaaggcgga aacccgcgcc tcgaagaacc gcacaatctc gtcgagcgcg
     3721 tcttcgggtc gaaggtgacc ggtgcgggtg gcgatgccat cccgctgcgc tgagtgcata
     3781 accaccagcc tgcagtccgc ctcagcaata tcgggataga gcgcagggtc aggaaatcct
     3841 tggatatcgt tcaggtagcc cacgccgcgc ttgagcgcat agcgctgggt ttccggttgg
     3901 aagctgtcga ttgaaacacg gtgcatctga tcggacaggg cgtctaagag cggcgcaata
     3961 cgtctgatct catcggccgg cgatacaggc ctcgcgtccg gatggctggc ggccggtccg
     4021 acatccacga cgtctgatcc gactcgcagc atttcgatcg ccgcggtgac agcgccggcg
     4081 gggtctagcc gccggctctc atcgaagaag gagtcctcgg tgagattcag aatgccgaac
     4141 accgtcacca tggcgtcggc ctccgcagcg acttccacga tggggatcgg gcgagcaaaa
     4201 aggcagcaat tatgagcccc atacctacaa agccccacgc atcaagcttt tgcccatgaa
     4261 gcaaccaggc aatggctgta attatgacga cgccgagtcc cgaccagact gcataagcaa
     4321 caccgacagg gatggatttc agaaccagag aaagaaaata aaatgcgatg ccataaccga
     4381 ttatgacaac ggcggaaggg gcaagcttag taaagccctc gctagatttt aatgcggatg
     4441 ttgcgattac ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc
     4501 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     4561 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgccacgctc accggcaaat
     4621 taggagcgca gcgagtaatt tgtccgtgtg tagcgtattg ttaggcgctt gtgccttgcc
     4681 agcgacgata caggctggca atgccagacg aacaaagaaa aggcattgct tccttgattt
     4741 gttccagcgg ccaatcccac caagccatat ctaaaagcat agaaatttct tcttcagaaa
     4801 agcgcttcct aatcgacttt gcagggtttc cccccactat ggtgtagggt tccacgtctt
     4861 tggcaaccaa agcgcggcta cctatcaccg ctccatgccc gatcttgatc ccgggcatga
     4921 tcatggcctc cgaaccgatc cacacatcac ttcctataac tgtgtcgcca gcccgctgga
     4981 atgcatcgac tgattttgca aacgcgggct cctcgttcat gtagaagaaa gggaaagaag
     5041 agacccaatc atatcggtgg ccttgattcc cagccataat aaaagctgcg cctgatccga
     5101 tggagcagaa gctgccgata atcagctgat caacgtcatc acggtctggt agaaggtagc
     5161 gagcacaatc atcaaacgag tgcccatggt aatagccgga atagtagcta taccgcccta
     5221 ccttgatgtt cggattcttc acctgctcag tcagaagctt ccctttgaag ggactctcaa
     5281 aataattcgt cataaaattt gtacccgaaa attctagagc gactccacgc gtcgcctaac
     5341 atgtattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac aaattcttcc
     5401 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     5461 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     5521 tccttcggcg cgattctgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     5581 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     5641 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     5701 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     5761 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     5821 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     5881 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     5941 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     6001 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     6061 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     6121 atcaccgctt ccctcatgat gtttaacgcc tgagttaagc cggagcgctt tgcggccgcg
     6181 gcgttgtgac aatttacaga acaactccgc ggccgcgaag cgatctcggc ttgaacgaat
     6241 tgttagaccg cgcttagaag aactcatcca agatccggta gtactcaatc ttagctatgt
     6301 tcggatttgg ctctccatac tcgatgaaga actggttagt gagttctggt ccaaataccg
     6361 cgtcaatgtt tctcgatgca agcgccaggt cttgatactt atcggcaagc ccgacccgac
     6421 cgcagtctat gaagccgacg aaggcattac cttggaagat gaagttctcg ggacaggcgt
     6481 cgccatgtgt gactaccagc tgctccgctc caggcatttg tataaaaagt tgctcgtaaa
     6541 catcccgcgc cagcatgcct tgccttgcgt gatcaaagtc ctcctcatca actaacccgg
     6601 cttccaaacg gcccgaagcc agcttcagtc gcaggtggag cctttcgtcg aatgggcaat
     6661 cgtcaggcga gatcgaatgg aggtcgcgta acgctcgtgc catctctgca acaacaataa
     6721 ctggatctgc atctgcggcg tcagcggcat tgtggccaat caatgctttt gtcaacagga
     6781 tggtgacagt tgaagtcgag acatatgaga tcacttccgg tacgagagct cgtgttctga
     6841 gccatctagt gcgctgatgc tcttgaagaa tttcttgagc agaagatccg gaggcgtact
     6901 ttagatacgc ggtattgcga tcaggaaata gaactcgaat cactcgagca tctgactgac
     6961 catactcaac ttcttcaaaa gtgcattcat ccaacaaggg tgaaagcaaa ttctttagtt
     7021 cagatacttc gtcgagggcg actgtcatag cggtctaacg tttgacatga ggggcggcca
     7081 agggcgccag cccttggacg tccccctcga tggaagggtt aggcatcact gcgtgttcgc
     7141 tcgaatgcct ggcgtgtttg aaccatgtac acggctggac catctggggt ggttacggta
     7201 ccttgcctct caaaccccgc tttctcgtag catcggatcg ctcgcaagtt gctcggcgac
     7261 gggtccgttt ggatcttggt gacctcggga tcattgaaca gcaactcaac cagtgctcga
     7321 accagcttgg ttcccaagcc tttgcccagt tgtgatgcat tcgccagtga ctggtctatt
     7381 ccgcgtactc ctggatcggt ttcttcttcc caccatccgt ccccgcttcc aagagcaacg
     7441 tacgactggg catacccaat cggctctcca ttcagcattg caatgtatgg agtgacggac
     7501 tcttgcgcta aaacgcttgg caagtactgt tcctgtacgt cagcaagtgt cgggcgtgct
     7561 tcttctccgc cccaccactc gacgatatga gatcgattta gccactcata gagcatcgca
     7621 aggtcatgct cagtcatgag gcgcagtgtg acggaatcgt tgctgttggt cacgatgctg
     7681 tactttgtga tgcctaacgc ctgagttgag gggcttttgc ggagtaggcg cagcctgcgt
     7741 agcaaaagtc ccgctccaac gatttgttat gccgcatctg ctactcggcg actgagcgat
     7801 ttttgtgtgc tttgacaacg ttcgctgtgt gctggagcaa gtctagaccg cccggtagac
     7861 cgtgcccggg aatgacgacc tctgcttccg ggtagtgttt ttgaatccgc tcaacggagg
     7921 tgggccattc agccagatcg gcatcggcca cgttccccgc agacgtgctt gacaactcat
     7981 gaacggcaca accaccgtat agcacgttcg ctgacgggac gtatacaacc agattgtcgg
     8041 tcgaatgcgc agcaccagga tagaagagct ctactggacc gaagcgcact gcgtccccgc
     8101 tcgatgagag tccttctaga gaatgcgtgg gaatctcgtt cccctctgcc tcggctagcc
     8161 ggcgtgtcga cggtgatgcg tacgttgcca ccccagccgc ccgaaggaca tcaacgccgc
     8221 cgacgcggtc gtcatgaaag tgcgtggaga ctgcacgcgt tacgggaagt ccaatttgct
     8281 tttcaatctc cgcgagaagt gccgctgtgt ttttcgcacc ccacgctgta tcaatcaaaa
     8341 gcaactcatc accatcacgg acaatgagac cattggacgg gtagaccgcg ccatcaaacg
     8401 actgcgttgc gatatgcgac caaacaccat cggcaatctg gtaaagtcgg acctctccga
     8461 ccggaatttc gttgactgtc ggatactcac cactcggctc cccggaatgg gctaacggac
     8521 ttgcgacagc catgacagac gcggtcatgt agaccaataa actactaata acttttaaca
     8581 tcaagactcc ataggggtgg gtgcggcata actttgtttt agggcgactg ccctgctgcg
     8641 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     8701 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
     8761 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     8821 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
     8881 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
     8941 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
     9001 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
     9061 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
     9121 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca gcttctgtat ggaacgggca
     9181 tgcggatcag tgagggtttg caactgcggg tcaaggatct ggatttcgat cacggcacga
     9241 tcatcgtgcg ggagggcaag ggctccaagg atcgggcctt gatgttaccc gagagcttgg
     9301 cacccagcct gcgcgagcag ctgtcgcgtg cacgggcatg gtggctgaag gaccaggccg
     9361 agggccgcag cggcgttgcg cttcccgacg cccttgagcg gaagtatccg cgcgccgggc
     9421 attcctggcc gtggttctgg gtttttgcgc agcacacgca ttcgaccgat ccacggagcg
     9481 gtgtcgtgcg tcgccatcac atgtatgacc agacctttca gcgcgccttc aaacgtgccg
     9541 tagaacaagc aggcatcacg aagcccgcca caccgcacac cctccgccac tcgttcgcga
     9601 cggccttgct ccgcagcggt tacgacattc gaaccgtgca ggatctgctc ggccattccg
     9661 acgtctctac gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc ggagtgcgct
     9721 caccgcttga tgcgctgccg cccctcacta gtgagaggta gggcagcgca agtcaatcct
     9781 ggcggattca ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc cggttgcgga
     9841 aagtcctccc tgcgtccgct gatggccggc agcagcccgt cgttgcctga tggatccaac
     9901 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     9961 acatgtcgca caagtcctaa gttacgcgac aggctgccgc tgtcgttttc agaaggcgac
    10021 tgcaccagtt cactgggctg gccgccgtgt gtgcatagaa cttctgaccg ggaacggtca
    10081 gaagttctat gcacgaagcg ccgcgagaga tcggaattgc tcagaactct tgtgcacggc
    10141 gcgacaaagc cggaaccgcc gaaaattccg gcgatcaacc ggcaagctgc ttgatgcgct
    10201 cggccaggcg gccgaaagcc tcgttcacgt attccagcac cttgggttct gcgccctgcg
    10261 tgcgcagcga gtctttgaag gcttccaggt cggacagcca ttgccgatgc aggcggccca
    10321 gctcgtcgag ctgaggccgg aagtcttgga gactgccgct cgcacgaaac gcctggtagc
    10381 cacgctcaag cgtggccgcc gtggtcagca gttgcgcgcc catttgcttg tgctgttcgg
    10441 agaactcggc catgccgtca tagggccgcg aagccttgcg gcgctcggct tcggatgcct
    10501 cttccatgat gcgggcgtag cggtagaacc ctgggaactc gcgggacagc gccatgagct
    10561 gctgatcgga tgtgccgacc caaatcctgt gcaggtcagg cccgtacccc atcatgcgat
    10621 tgatgatggc atgggcctcg ctgacaccct gggcggcaag ctgctgcatg tgttggtcga
    10681 tcttcgcggc cagtcggcgg aattcgttca tgctcatgtc cgtcacaaaa ggcgcgttga
    10741 ttgcccttgc gccgtgagaa gtgccaccgc cttcttatcg aaggaaacga aggtttcccc
    10801 gccaagccag ttgccttcgt aggcaatgac gccatcggca aagtctccgc ccgcgtcgag
    10861 caccagcaag ccagcctcca cggcaggccg gttcacttcc acattcgcgg cggccagtag
    10921 tgcccggatc gcgctggccg cgtcggcttg ctggaagccg tagacacgca gcagcaccca
    10981 aacaaattcg cataggcacg gcagcgcgac cgcgatcaac tcggcgtcgg tcaagactgc
    11041 ggcggcaacg tccgcttgtg cgggatcgtc acgcacaacc gcacgcacaa ggacgttggt
    11101 atcgactgcg accttcattg cttacctgcc cagccttgcg ccgccgcctc gttgatttct
    11161 tcgatggtgg caaccttctg cgtcttgccc gcgagcaggc cgacaaagct ggctatcgtc
    11221 cctgcgggcc gtgccgcctt gagcacgccc cgaccgtctg gcaacaagtc cagctcgatc
    11281 ttgtcgcctg gcctgatgcc gaggtgttgc agtacgtcct tccgaaacgt cacttgtccc
    11341 cgtgcggtaa cggtcaatgt ggtcatggtg gtttgccctc gcaatcaagg ttgatgcttc
    11401 acatagtaat gcaaaaatgc cttaccgtca atgtccgtca aacgatcatt agtcggccat
    11461 gccggacact gtccagcaaa gttattgcac aacgggttgt cggacattaa gataggcgga
    11521 cggaatgacg gacaagagag gcggcaaatg gcactgatcg gctatgcgcg ggtatcgacg
    11581 gcggaacagg acaccgcctt gcagacggat gcgctacgca aggcaggctg cgagcgcgtt
    11641 ttcgaggaca cggcttccgg ggccaaggcc gaccgccccg gcttggctga tgcgctggcc
    11701 tacctgcgcg acggcgacgt gctggccgtc tggcggctgg atcggctcgg gcgctctatg
    11761 ccgcacctaa tcgaaacgat aggcgcgctg gaagcgcgag gcgtcggctt ccgttctctg
    11821 acggaagcca tcgacaccac cacgccaggc gggcggctca tcttccacgt gttcggcgcg
    11881 ctgggccagt tcgagcgcga cttgatccgc gagcgcacca aggccgggtt gactgccgcc
    11941 gccgctcgtg ggaggaaggg cgggcgaaag ccggttgtca ccgccgacaa gttgcagcga
    12001 gcgcgggagc acatcgccaa cgggcttaat gtccgagagg ccgctacacg gctcaaggtg
    12061 agcaagacgg cactgttgca aagttagcga tgaggcagcc ttttgtctta ttcaaaggcc
    12121 ttacatttca aaaactctgc ttaccaggcg catttcgccc aggggatcac cataataaaa
    12181 tgctgaggcc tggcctttgc gtagtgcacg catcacctca atacctttga tggtggcgta
    12241 agccgtcttc atggatttaa atcccagcgt ggcgccgatt atccgtttca gtttgccatg
    12301 atcgcattca atcacgttgt tccggtactt aatctgtcgg tgttcaacgt cagacgggca
    12361 ccggccttcg cgtttgagca gagcaagcgc gcgaccatag gcgggcgctt tatccgtgtt
    12421 gatgaatcgc gggatctgcc acttcttcac gttgttgagg attttaccca gaaaccggta
    12481 tgcagctttg ctgttacgac gggaggagag ataaaaatcg acagtgcggc cccggctgtc
    12541 gacggcccgg tacagatacg cccagcggcc attgaccttc acgtaggttt catccatgtg
    12601 ccacgggcaa agatcggaag ggttacgcca gtaccagcgc agccgttttt ccatttcagg
    12661 cgcataagcg gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc agcatctcct
    12721 gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt
    12781 cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat
    12841 gataagttta tcaccaccga ctatttgcaa cagtgccaac gccgggttat tcttatttgt
    12901 cgcttcttta ctcgccttta tcggccttca ctcaaggatg tattgtggtt atgcgttata
    12961 ttcgcctgtg tattatctcc ctgttagcca ccctgccgct ggcggtacac gccagcccgc
    13021 agccgcttga gcaaattaaa caaagcgaaa gccagctgtc gggccgcgta ggcatgatag
    13081 aaatggatct ggccagcggc cgcacgctga ccgcctggcg cgccgatgaa cgctttccca
    13141 tgatgagcac ctttaaagta gtgctctgcg gcgcagtgct ggcgcgggtg gatgccggtg
    13201 acgaacagct ggagcgaaag atccactatc gccagcagga tctggtggac tactcgccgg
    13261 tcagcgaaaa acaccttgcc gacggcatga cggtcggcga actctgcgcc gccgccatta
    13321 ccatgagcga taacagcgcc gccaatctgc tgctggccac cgtcggcggc cccgcaggat
    13381 tgactgcctt tttgcgccag atcggcgaca acgtcacccg ccttgaccgc tgggaaacgg
    13441 aactgaatga ggcgcttccc ggcgacgccc gcgacaccac taccccggcc agcatggccg
    13501 cgaccctgcg caagctgctg accagccagc gtctgagcgc ccgttcgcaa cggcagctgc
    13561 tgcagtggat ggtggacgat cgggtcgccg gaccgttgat ccgctccgtg ctgccggcgg
    13621 gctggtttat cgccgataag accggagcta gcaagcgggg tgcgcgcggg attgtcgccc
    13681 tgcttggccc gaataacaaa gcagagcgca ttgtggtgat ttatctgcgg gatacgccgg
    13741 cgagcatggc cgagcgaaat cagcaaatcg ccgggatcgg cgcggcgctg atcgagcact
    13801 ggcaacgcta acccggcggt ggccgcgcgc gttatccggc ccgcagcacc tcgcaggcgt
    13861 gccgggcgat atgactggcg gcggcatcgg aaagatgccg gtcggtaatg atggtggtga
    13921 accgggtcaa aggtaacgcc ataaacgtgg ccacctgatt gtatttcgaa ctgtcgcaca
    13981 gcaggatgct tctcgcgctg acctggctga cggtctcctt gacggtaacc ttgttctcat
    14041 caggggtgaa tatcccgcga ctgtcccagc cgctggcgga gataaaggcc gtatcgatag
    14101 ccaggtggcg taacgtacgc gccgccgatt cgcccacgca ggagcggttc tcccggcaca
    14161 gagtgccgcc ggtgtggatc acgccgcact ggctggcatc gatcagcagc tgggttatct
    14221 caaaatcatt ggtgaccacc tggagatcgt tccggtcgag gatcgcccgc gccagcgcca
    14281 gggtggtagt cccggcatcc agatagatgc aactgttttt agcgatatga ctcgccgcca
    14341 gcgcgccgat cgcctgtttc tcctcactct gcagcgtgct tttcaccaga tgactgggtt
    14401 ccgcggccag ccggctgacg gcgcgtacgc cgcccgagac gctgaccagc agcccctgct
    14461 cctccagttt actgacgtcc cgacggatgg tcatatggga cacgccgagg atctccgtca
    14521 gctcgttaat gcttaccgcc ccacgctgct ccaccagggc taaaatacgc tgatgtcgtt
    14581 ctattggaat caccgctctc cccttaccat tttttcacac caggcgtcac cacccaggct
    14641 acggccctgg cgacacccgg tgttgttatc gcgctttgcc gctgtttttt acgctatttt
    14701 agggcaagaa tcgccgctgt gcagcctctt tgggggaacc gcagaattcg gaaaaaatcg
    14761 tacgctaagc taacggtgtt ctcgtgacag ctctttgact aggctttcta aggccattct
    14821 gatagccctg acttcctgaa aagccatggc taaaatttgt gcggctaaaa gggataaccg
    14881 atggtaaagt aagttatccc tgtcgagata ctgaaaagcg ttatcctcgt ttttcccaaa
    14941 actgttttgc cagttcgctc agagcgctag ttaactgagc gacagatttc gcactttgca
    15001 aattattctg cccggtctgt acattggtat cagcagcatc gcgtatattg ataatactgc
    15061 ggtttatgtc ttcacttact gctccctgct gctcgaccgc agtcgctatt tgcgcgttca
    15121 tgtcggtaat ttcgttaacg cgttggccaa ttccatcaag agctgtagct gcttcctctg
    15181 cgtgagctac actcgtgtgc gcttgccgac tactttgctc catgactgta acagcggatt
    15241 gcgctcgctc ttgtagagcg ctgatcatgc tttgaatatc cgttgtcgat tgctgtgtgc
    15301 gagcagcaag actgcgaacc tcatcggcga caacagcaaa accacgcccc tgctcaccag
    15361 cacgcgcggc ctcaattgct gcgttgagtg ccaacaaatt cgtttgctcg gcgatccctc
    15421 gtataacgtc aagaactttt gatatctcgt tactttgacc ttcaagctca tgaataacct
    15481 gagtggcttg cctaatttca ccttcaaggg cagtgattga ctggcttgtg tgggctacca
    15541 gacgctggcc agatgccgtc tcagtgtctg ctcttccggc cgcatctgca gcatgctgtg
    15601 cattgctcgc aacctcttga atgcttgcca ccatttggtt tactgccgtt gctatttgat
    15661 ctgtctctgc ctgctgctca actgtaagta cattgcttga ctcaatatcc tttagtaggc
    15721 ctcgggtgtg ttcgctaagc cgatttgatg catcacctat gcgacctact atggcgcctg
    15781 tttcagcttg catcattcgt aaagcaaact ctatttggcc aaactcatcg gtgcgcccag
    15841 tgtagaggga ttgacttaat gggttattgg aaatattcct ggctctttca accagtcttc
    15901 caagaggaga gagaatagcc aaaacactaa cagagcttaa gcttcctgac attaaagtgg
    15961 ctaacaataa gctgcttatt gatgtatcag taagcatgcc ggcagccatt gcgcttgata
    16021 taatactacc ccatatgagc aagagtattt tcacggaaaa gctagcagcc aatttcggcc
    16081 tcgcggcctt cccgcttctc aattgagcat ataatttttc cgcagccaaa acctgctcag
    16141 gttcaggctt ggtccttaca gactggtatt caacaatcga accattctta gctattggcg
    16201 ttacataagc acttacccaa tagtggtcgc cattttggca ctgttgcaaa tagtcggtgg
    16261 tgataaactt atcatcccct tttgctgatg gagctgcaca tgaacccatt caaaggccgg
    16321 cattttcagc gtgacatcat tctgtgggcc gtacgctggt actgcaaata cggcatcagt
    16381 taccgtgagc tgcaggagat gctggctgaa cgcggagtga atgtcgatca ctccacgatt
    16441 taccgctggg ttcagcgtta tgcgcctgaa atggaaaaac ggctgcgctg gtactggcgt
    16501 aacccttccg atctttgccc gtggcacatg gatgaaacct acgtgaaggt caatggccgc
    16561 tgggcgtatc tgtaccgggc cgtcgacagc cggggccgca ctgtcgattt ttatctctcc
    16621 tcccgtcgta acagcaaagc tgcataccgg tttctgggta aaatcctcaa caacgtgaag
    16681 aagtggcaga tcccgcgatt catcaacacg gataaagcgc ccgcctatgg tcgcgcgctt
    16741 gctctgctca aacgcgaagg ccggtgcccg tctgacgttg aacaccgaca gattaagtac
    16801 cggaacaacg tgattgaatg cgatcatggc aaactgaaac ggataatcgg cgccacgctg
    16861 ggatttaaat ccatgaagac ggcttacgcc accatcaaag gtattgaggt gatgcgtgca
    16921 ctacgcaaag gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg
    16981 gtaagcagag tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta
    17041 actttgcaac agtgcc
//
