LOCUS       NZ_CP107607            11625 bp    DNA     circular CON 18-NOV-2023
DEFINITION  Acinetobacter baumannii strain 1326924-1 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP107607
VERSION     NZ_CP107607.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30898357
            Assembly: GCF_025854155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Hirschi,B., Sangster,A., Hergert,J., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     1326924-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Hirschi,B., Sangster,A., Hergert,J., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/18/2023 05:30:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,031
            CDSs (total)                      :: 3,936
            Genes (coding)                    :: 3,845
            CDSs (with protein)               :: 3,845
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 53 of 91
            Pseudo Genes (internal stop)      :: 15 of 91
            Pseudo Genes (multiple problems)  :: 7 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107607.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="1326924-1"
                     /isolation_source="ENVIRONMENT"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA"
                     /collection_date="2018-10-14"
     gene            1..951
                     /gene="repM"
                     /locus_tag="OIO60_RS20105"
                     /old_locus_tag="OIO60_20110"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="OIO60_RS20105"
                     /old_locus_tag="OIO60_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208776.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="OIO60_RS20110"
                     /old_locus_tag="OIO60_20115"
     CDS             944..1519
                     /locus_tag="OIO60_RS20110"
                     /old_locus_tag="OIO60_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096613.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAVPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1531..1674
                     /locus_tag="OIO60_RS20115"
                     /old_locus_tag="OIO60_20120"
     CDS             1531..1674
                     /locus_tag="OIO60_RS20115"
                     /old_locus_tag="OIO60_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1980..2255
                     /locus_tag="OIO60_RS20120"
                     /old_locus_tag="OIO60_20125"
     CDS             1980..2255
                     /locus_tag="OIO60_RS20120"
                     /old_locus_tag="OIO60_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_001133626.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            2277..3386
                     /locus_tag="OIO60_RS20125"
                     /old_locus_tag="OIO60_20130"
     CDS             2277..3386
                     /locus_tag="OIO60_RS20125"
                     /old_locus_tag="OIO60_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_000842148.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            3439..3837
                     /locus_tag="OIO60_RS20130"
                     /old_locus_tag="OIO60_20135"
     CDS             3439..3837
                     /locus_tag="OIO60_RS20130"
                     /old_locus_tag="OIO60_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            4311..5144
                     /gene="fghA"
                     /locus_tag="OIO60_RS20135"
                     /old_locus_tag="OIO60_20140"
     CDS             4311..5144
                     /gene="fghA"
                     /locus_tag="OIO60_RS20135"
                     /old_locus_tag="OIO60_20140"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            5655..5942
                     /locus_tag="OIO60_RS20140"
                     /old_locus_tag="OIO60_20145"
     CDS             5655..5942
                     /locus_tag="OIO60_RS20140"
                     /old_locus_tag="OIO60_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            5929..6243
                     /locus_tag="OIO60_RS20145"
                     /old_locus_tag="OIO60_20150"
     CDS             5929..6243
                     /locus_tag="OIO60_RS20145"
                     /old_locus_tag="OIO60_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6371..8782
                     /gene="znuD2"
                     /locus_tag="OIO60_RS20150"
                     /old_locus_tag="OIO60_20155"
     CDS             6371..8782
                     /gene="znuD2"
                     /locus_tag="OIO60_RS20150"
                     /old_locus_tag="OIO60_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            9087..9548
                     /locus_tag="OIO60_RS20155"
                     /old_locus_tag="OIO60_20160"
     CDS             9087..9548
                     /locus_tag="OIO60_RS20155"
                     /old_locus_tag="OIO60_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(9871..10044)
                     /locus_tag="OIO60_RS20160"
                     /old_locus_tag="OIO60_20165"
     CDS             complement(9871..10044)
                     /locus_tag="OIO60_RS20160"
                     /old_locus_tag="OIO60_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(10044..10415)
                     /locus_tag="OIO60_RS20165"
                     /old_locus_tag="OIO60_20170"
     CDS             complement(10044..10415)
                     /locus_tag="OIO60_RS20165"
                     /old_locus_tag="OIO60_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(10686..10940)
                     /locus_tag="OIO60_RS20195"
     CDS             complement(10686..10940)
                     /locus_tag="OIO60_RS20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.264"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagttgtaaa agacaatgcc ttaattaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgctaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactccta ttatagaggt aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atctgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctatcc gcaatttgct
      841 attcgtattg ctgagatgct gcaagaccct gaccgaataa aagaactata cccataccta
      901 aaaaaagtgg gatatatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat tccatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tccgatgctt
     1081 tggagaacct gagcaaacac cccaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca tcaccacaac aagatgctac ggcatgcgac actgacatgc aacagcatgc
     1321 gacaccacaa caagacaatg ataataaaaa gaatcatgaa ttaaatattg cagaaaatgt
     1381 ggcagtgcca cagcaagaaa atacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaaatgtg gcagtgccac aacacaaaaa acgtggctta tttggccgtg tgctgaatgc
     1501 tgtttttgat aatgactaag ggaggggatc atgccgaaac tgaaagatat tgccctggga
     1561 attattgtag ctccgctgct gatcccgatc atgctgattg catcgtacca ggataaaaag
     1621 gcgctgaaga aagagctgga tgaatgccaa aaagaaaaag accaggcatc ctgattattc
     1681 aattgagcta atcaaaagtg tggaacattg atgaaataat agcgtggaac gtgaaaagtc
     1741 ataaaaaaag cccgaacagg gatgctcggg ctataaacta gaaactacag cgttgaatta
     1801 taaggataat tatacagcat ttcgtataag gtgtattatg ttaattttat aaaaacttaa
     1861 aaatagatat agaatttttt aattataagc ttttatcaat tattaatttt ataaaggctt
     1921 cttgtcattc tatatactat agggtactat actataataa aatttaagga attagcttta
     1981 tgccaaatca agtcgaagat aaaaaaaaga ttctgacgcg tgtcagacga atcaagggtc
     2041 aggctgaagc gattgaaaaa gctttagaag acaatgtgga atgtggtgca attctgcaac
     2101 aaatatgctc tgtgcgtggt gctattaatg gtttaatgaa tgaaatgttg gaggttcatt
     2161 taaaggacac tttagtatca ggtgaaacca cagaacaaca acgaaaagaa gaattggctg
     2221 aaatcgccaa gattctaaaa tcatatttaa aatagatctg tataaggaat tattccatga
     2281 agtcacgtgc tgctgttgcg tttggtcctg gtcaacctct tcaaattgtt gaggttgatg
     2341 ttgcaccacc gaagaaaggt gaagtattaa tcaagatctc ccatactggt gtgtgtcata
     2401 cagacgcatt tactttatct ggcgatgatc ctgaaggcgt ttttccggct attctcgggc
     2461 atgaaggtgc tggtgttgta gttgaggttg gtgaaggcgt gactagcgtc aaaccaggtg
     2521 atcatgttat tccactttac actgctgaat gtggcgagtg cgtattctgt aaatctggaa
     2581 aatcgaactt gtgtatttcc gttcgcgaaa ctcaaggtag aggcgtaatg ccagatggta
     2641 ccactcgctt ttcctacaat ggtcaaccga tttaccacta tatgggctgc tcaacgttca
     2701 gtgaatacac tgtagtggca gaagtatcat tggctaaaat caatcctgaa gccaatcacg
     2761 aacatgtctg cttactgggt tgtggggtaa caaccggtat cggtgcagta cataacacgg
     2821 ctaaagtaca agaaggtgat agtgttgcgg tctttggttt aggtggtatt ggtttggcag
     2881 tcgtacaagc cgcacgtcaa gctaaagcag ggcgtattat tgtggttgac atgaatccag
     2941 ataaatttgc gctggcaaaa gagtttggtg caacggactt cttgaaccct aaagactacg
     3001 ataaaccaat ccagcaagtc attgtcgaaa tgactggctg gggtgtagat cattcatttg
     3061 aatgtattgg caacgtgaat gtaatgcgtg cagcccttga aagtgcacac cgtggctggg
     3121 gacaatccgt aattattggt gtagctggcg caggtcagga aatctctacc cgtccattcc
     3181 aactggttac tggtcgtaaa tggctaggtt ctgcctttgg tggcgtaaaa ggtcgtacgc
     3241 aacttccagg aatggttgaa gatgccatga aaggtaaaat tcaattagaa ccatttgtga
     3301 cacatacgat gggcttggat cagatcaatg aagcctttga tctaatgcat gaaggtaagt
     3361 caattcgcac tgtcattcac tattgatcca cggatcaata cctaattttt caaaaccaac
     3421 catcaaggaa acactttaat ggctactcca gtaatttccg gtaacgatat tttctcccat
     3481 gtcttcctcg gcgcagtcga ccttaagaag tcggttgtat tctacgatgc cgctctaggt
     3541 gccctcggca ttaaaaacct agggccattc ggtaacgatt gggttctgta cggtcgcgac
     3601 aaacctgctt tcatgatcac ccgcccaagc aatggcgcag aacccaccag taatggtgtg
     3661 acggtaggct tcgcagctgc cacccctgca gaggtaaatg cattccacgc agccggcctc
     3721 gccgctggtg gcaccgatga aggtcagcct ggcccacgtg gccacctgcc gggtgcttac
     3781 gcggcctacc tgcgtgatcc ggctggtaat aaggtctgcg cctacacctt tgtctaaaac
     3841 ctaggagaag tgaggtcatt gtgtaaggac tggttccagc caaaaacaat ctcatcttcc
     3901 actgcaaccg tgtggcatgc ccgctcatgc cacacggttt acggtactct gagcctctat
     3961 ctgctgatta gagctcagaa aggctgttac ctcgccttca tagccaaaga cggtcaatga
     4021 gcacgatcgg actgaaagtt cggtgcgtga tgcaagaaac acagaatcca aaatgagcat
     4081 cgcggtagtg gctccctgaa ttacacactt tattgtttgt acattgaagt cgacatccga
     4141 ccttcgtggt gtggtggcgt ctgggtttgt agcagcgatc ttcatgccag cgacaacgct
     4201 gaaaacctgc tgagtggtgc ggtgaagcat ttttaactaa tcctggatgg aacgaaaatt
     4261 cgtcttgatt ggcgcctgag caccacctcg ttgaaataag gagaaaatcc gtggaacgca
     4321 ttgaacatca cgccagcttc gagggttggc aggacgtttt tcagcatgag tcaacagtgc
     4381 ttggctgcat gatgaggttt ggaatctatc ttccgcctca ggctgagcat gaaaagctcc
     4441 cagttctgta ctggctctca ggtctgacct gtaccgaaca gaacttcatc accaaggctg
     4501 cggtgcagcg gtatgccgcg gagcacggaa tcattgtcgt agccccagat accagcccgc
     4561 gcggagaaag cgttgcagac gatgctgcct atgatctagg tcaaggtgcg ggtttctatg
     4621 tagacgccac agagctacct tggagcgatc actatcagat gtattcctat gtggtcgacg
     4681 aattaccagc gctgatcgaa gcgaacttcc ctgcgtcgtc aagacgcagc atcagtgggc
     4741 actccatggg cggtcatggc gctctggtta ttgcgcttcg caaccccggt cgctatgcaa
     4801 gtgtctcggc gttctcgccg atcgtggcgc ctacgcaagt tccatggggg cagaaggctc
     4861 tggcggctta cttgggcgaa aagaatctgg aagcctggaa gcaatatgac acggtcgagc
     4921 tgattcgcgt ggcgcaagaa cgcttaccgc tgcttgtcga tcaagggctg agcgatgagt
     4981 ttctcgagca ccagcttcga ccgcaactgt tgcgtaccgc gtgtgaacag gtagaccacc
     5041 cgctaacgct caatctgcgg cctggtcatg accatagcta ctactttatt tcgacctact
     5101 tgagcgaaca catggcccac catgcggctg cattgaatcg ctgaacattg ggagaccctg
     5161 acttggaaat gtctgtatag gacatttcta tttggtttta aggggctttg ttgcataaat
     5221 acataaccaa ctgattttgt tcatttaatt tttaggttga aattcataca aacctattta
     5281 aatcagaagc ttacaaaatc tttatgcaac aaagcccata tcagataaaa aagtagagga
     5341 gctaatacta agccgtgatt tcttggaaaa gctgaaagag ctagttaatc gaacagaaat
     5401 accatgtagt taactatcca tttaacataa tggatattat acgaagtaca tttgtatatt
     5461 aatttaaata tcaactactt aataaaaata taaaaatcca aaaatgccca aaaagccgac
     5521 tttgaaacaa gtcggctttt ttatgatcga ttttgatagt ttttgccaat aaatctgact
     5581 aaaaattacg cttctcaact tgagtgatac aatttcgtga tacattattg tatatacaaa
     5641 tttgaatgtt ttttatggaa cagtattttg aatgggatga ggctaagaat cgaaagaatc
     5701 agaaaaagca tgatatctct ttcgaaactg caagccttgt ttttgaagat ccattacgga
     5761 tctcaatcca agacagatat actaatggtg aagaacgttg gcaaaccatt ggacgagtta
     5821 aaggcgtact aatgttattg gtggctcaca ccatttttga tgaagatgac tgtgaaatca
     5881 tccgaatcat tagtgcaagg caagtcacta aagcggagcg aaacaaatat gagcatggtt
     5941 agatactcac acaaagagct gaatgagaaa tttggtgaga agcaagatgc tgaaattcaa
     6001 cgcttgcttg ctaaaggcac ggttcctgat gatcagctag atctatcaga tattcctgaa
     6061 attacagact ggagcaatgc tgtacgccaa aatcaatttt accgcccagt aaagcaacaa
     6121 acttcaattc ggttagatgc tgatgtactt gcttggttca aagcacaagg caaaggttat
     6181 caaacacgta tgaatgaaat tttacgagat gctatgctaa aagaattaaa aaatcatcaa
     6241 taagaatggg agcttaggct cccatttttg acttcatttt taggggggat taccattcaa
     6301 aaatttaaat agtagatttt ataaattttt aagtgttata ttataatata acattaaatt
     6361 tggttgtttt atgctaaata aaagtaaact atttttagct ttaattactt taggggcctc
     6421 gaaaatttta ttggcagctg aagggcctgt tactacctta aatacaatag tattaactgc
     6481 acaatctgat gagttagggt ctgagctgtt aggcaaaagt ctaaatgtta gtaatcagtt
     6541 tattgatact agtaaactta aacagcgttc gacaactcta ggggatgcct tgggtacgga
     6601 attaggaatt cattctaatc aatatggagg tggagcatct actcctatta ttcgtgggca
     6661 ggaaggaaaa cgtattaaag ttttacagaa taatgctgac gtgttggata tgtctaatat
     6721 gtctccagat cacgctgtca cagttgaacc aagtttagct aagtctattg aaattatacg
     6781 tggtgcaagt acccttttgt atagttcaaa tagtgcggca ggggtagtca atgtcattga
     6841 ctataaaatt ccaacccaaa tgcctcaaga cggattagag ggtaatacta cattacgttt
     6901 taatactggc agtaatgaga agttgaccac agcaggtgta acagttggct taagcccaca
     6961 tgtagcttta cgggcagaag ggttatatcg taatgctggt aactataaaa ctcctcatta
     7021 ccagtctagc tcttataatt ctttagaaga tcttgaaaac caaaacagta tttataaaaa
     7081 tttaaaatat ttaccagaaa gctgggcaga gtcgagagta ggtacgcttg gattatcctg
     7141 gatagacgat aatacttatt taggagtttc ttatacacat cgtcatgatg aatatggttt
     7201 accagcccat agccatttat atgaaggttg tggtgcctct gcaatcggta tcgattcacg
     7261 aattagtgga ttaaaaaatt atcttttata ttatcctcaa ttgatggatg agcaagatat
     7321 taattacata aatccacgtc ctgattgtca tcagcataat catattcatg aaactaattt
     7381 ctctcataat gctccttata ttgatttaaa tactcgacgt tatgatgtgc gtggagaatt
     7441 cactcaacca tttaccggta ttgacaaaat tcgtacctca ttgagttata tagattattt
     7501 tcacaatgaa ctcgagggag ataagattac taactttttt aaaaatacag gaaaagtagg
     7561 ccgcatagag ttatcacatc agccattagg ggaattaacc ggtatcttgg ggctacaata
     7621 tttggaacag gataatagtg ctttatcacc agtccattct caagaaggcc ataccaccta
     7681 tctagataat cagcagctat taaatcgaaa tgtcactaaa aactttagtg tatttggcct
     7741 agaaaaatat aactggaacg actttacttt cgaactaggt gctcgtatag aaaagcagaa
     7801 agttagtatg gattatgata tagaaaaaat taaagattca atgaaacctt ggcctaataa
     7861 atataacagt ccttatgtag aaaaaaataa taaaatcagg gctcaaaatt taaaatctat
     7921 tttagaagct gttcaaccta acaaagaaac agctttttct tatgcaggta cagtccactg
     7981 gagatttgct ccaaattata tcttatcatt aacaggtaca catcaagaaa gattacctaa
     8041 tgcacaagaa atgtatactc atggtatgca tctcgctact aattcttttg aaataggtaa
     8101 ccgctttcta agaaaagaga aatccaataa tttagaaatc agtctggcct ataaggatga
     8161 tttacttgat tatcaaatca gtacttacta ttatgatttt gataactata tatatttaca
     8221 gacactcaat gaagttttag gaactacaaa agttagagat cagcatacac tacgtattaa
     8281 tcattatagc cagtctgctg ctaattttta tggattagag ggcaacatag ggtatcaatt
     8341 taattctgtt tatcacggct ctctttttgg agattatgtg aaaggccgtt taacaaattt
     8401 acctgatgct gtaattgcat acgatatctg gaatagagaa ccgactttag ctccccaaaa
     8461 ggatcgttac actcctagat tacctcctgc tcgtttgggt acacgactaa aagctgattt
     8521 tgatgaatcg cttaaaggtg aaatagaata ttatcgagtc tttaaacaag ataatatttc
     8581 taaatttgag caggttacat caggttataa tatgcttaat atgactttag catataaaaa
     8641 taagctatct cacacagaat atgatttatt ctttaaagcg aataatttac ttgatcagaa
     8701 agtctatgcc catgaaacat ttttgccata tattccccaa attggaagaa attttagttt
     8761 aggcttaaat cttaatttct agataagata ctgtaatctt ctaagaaaat ttcttagaag
     8821 atttttctta taaaatgatt agattaataa ttaaggtaag cgtccgctga atcccatacc
     8881 tattttttaa ttcgatttag gtttccagca gtgtggcagt aactcttcaa tctgggtcac
     8941 tttatgtgtc ggcagccttt tcagcactga atagccaccg attttgtaga caccttttag
     9001 ctagataaaa agcaattttt ttgatcttta aagaatgagg atataagctg tttaaaactt
     9061 aattctaatt ggttaatagg tgaaaaatga aattagcaga agctctctta cttcgaagtg
     9121 atcaacaaaa aaaaattatt tcactaaaac aacgtatcaa tgcaaatgta ttggtacaag
     9181 atggtgatca accatctgaa gatccaaatg aactactaaa acaagtattt tctttaattc
     9241 aagaatttca aaaattaagt tatgcaattc atgaaactaa tgctttaaca aaattaaatg
     9301 atggccgttc tctacttgct ttattgactt tgcgtgatga atttgtagaa cagcacaaaa
     9361 ccctaactgc agcaataagt aatacatctc gtgaatctga tcgttatagt acgcgggaaa
     9421 taaagtggca taaagttatt ccagtttcat cattacaaaa acaagctgat gatattagtc
     9481 ttaaattacg tgatttaaac gtgttaattc agtcgactaa ttggaaaatt gatttgattg
     9541 aagcctaata gtcctttaga tataatcaag ttgaacatac tgggcgagta caagatccct
     9601 tacttccaca acataggatt gtaaataaag tgtagtggct tgcaaagcaa gtggataagc
     9661 ttaaaatttt aagtacaaat caatccatca cttggcataa ctcaaatctt tgtgcaaaca
     9721 aagttacacg ttacttataa ctaccatgta ctgacagtat gttcacctca attactaaaa
     9781 ataaagaaat gagcaagcat cagctcaact acatttctat ttttaattca gttcggcatt
     9841 taacataaaa tctcttatgc gaagcccagg ttaatcctgg gctttttgtt caatctcatc
     9901 attggccgag aacatacttg gattcgtaag cctgatattt tcctgacaac ttccacacag
     9961 catcaatcct gcaaaaagtt gtacattttg tctattttca caaagcgtac atggctttga
    10021 aagctgaata atattatctg acactacttt accctcttat agttatcatc tgcatatgaa
    10081 taacctttag cttgcatcat tcgaacctca tctttagaag ccccattttt cattaatgtc
    10141 cataacatca tgaaatagag tccagaaaaa tcttcatagt atttttcaga aaaattatta
    10201 tcttcctcaa aatcatctat tcgcttttct aatcgattca ccagatcttc aatacgatct
    10261 gcctgctgct taacagcaat agattgaaaa tcattagtca aaattttact gaacaattta
    10321 ctggttctga ttccttctga attagccaat tgatcgtaga attctgctac ttgtggatct
    10381 agtctgactg cataagtttt gactttttcg cccataaatt acatctctta taaaaattag
    10441 agtttgtaca agattaaatc aagataattc aataatttaa attaaaataa taagtaaatt
    10501 acgtacaaat ctatttgtac aaaattaatc tattctaaaa caaaaactta tttaaatata
    10561 atgctttttt tatgtacaac tatgagtaca tgacttttat taaatttatc aatactttat
    10621 ctaaaaaaag tatggttttc atgtacaagc atgtgcacag cataaaaaac atataattca
    10681 actacttatt ctaaaaaaag accatttttc tgtacacgtt tctgagaccc taaaaaatcg
    10741 ctgaaatcct tatcctgtaa ggctttgagg ggtttcatcg ttagatgcgt ggtgtgtgac
    10801 aaaaaaatcc cggcatgtgc cggatcttgg attggaaaat tggctaaagt gacgtagggc
    10861 cggtgcttgg ttttgcatgg aaaaaagtat ttatttcctt gtttagaaaa acgtaaaaaa
    10921 tcaatttttt gtccattcat ccaggttaaa aatttcaacc caaaacttta attatgaaaa
    10981 gcttcacaga aagcgttcaa atgcgattta agagccttta cttaaaaaac atagatctca
    11041 tagtgaaaaa cagaaaacag cccaaaaaac gcaaatgaga acgaaataaa gagagatttt
    11101 aactttagat agatgcatag agcgagtgtc tacgagcgaa ctatcaaaat ttgcgcctag
    11161 accctctgaa aaacattttt ttgtcctctt tagcctaaga aagcgtaagt ttcatacaga
    11221 aatttgctcc tggatcgagc gtagcgagta aaaaagtttt atgagcgaag cgaattccga
    11281 gttgcttttg ctttttctta aagtcacgca agtattaacc aaaaaattgc cccaacgaac
    11341 tgagcgaaag cgaagttcaa tagagtttga gcgaagcgaa aaccaagggc aatttttcat
    11401 tccctgggct tttaattatt tttaatcttt taaatgcttt tagacatgct gaaagcctta
    11461 atttacaaca ctttcaaagg ctatatgaca ccatttacct tgcaatatga caccgtttac
    11521 cttgcaatat gacaccgttt accttgcaat atgacaccgt ttaccttgct tatagcacta
    11581 aaattaatta ttgtgtcata gcataataaa taatactggt gcttc
//
