LOCUS       NZ_CP107456            11947 bp    DNA     linear   CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_14 plasmid unnamed4.
ACCESSION   NZ_CP107456
VERSION     NZ_CP107456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207043
            Assembly: GCF_028223615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:33:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,826
            CDSs (total)                      :: 5,706
            Genes (coding)                    :: 5,551
            CDSs (with protein)               :: 5,551
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 53 of 155
            Pseudo Genes (incomplete)         :: 102 of 155
            Pseudo Genes (internal stop)      :: 17 of 155
            Pseudo Genes (multiple problems)  :: 17 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11947
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_14"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            347..1834
                     /gene="ltrA"
                     /locus_tag="OH499_RS28935"
     CDS             347..1834
                     /gene="ltrA"
                     /locus_tag="OH499_RS28935"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            1939..3624
                     /locus_tag="OH499_RS28940"
     CDS             1939..3624
                     /locus_tag="OH499_RS28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3634..3891
                     /locus_tag="OH499_RS28945"
     CDS             3634..3891
                     /locus_tag="OH499_RS28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3976..4125
                     /locus_tag="OH499_RS28950"
     CDS             3976..4125
                     /locus_tag="OH499_RS28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5609..7573
                     /locus_tag="OH499_RS28955"
     CDS             5609..7573
                     /locus_tag="OH499_RS28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7573..8304
                     /gene="mobC"
                     /locus_tag="OH499_RS28960"
     CDS             7573..8304
                     /gene="mobC"
                     /locus_tag="OH499_RS28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8311..8841
                     /locus_tag="OH499_RS28965"
     CDS             8311..8841
                     /locus_tag="OH499_RS28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8868..9047)
                     /locus_tag="OH499_RS28970"
     CDS             complement(8868..9047)
                     /locus_tag="OH499_RS28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9073..9501)
                     /locus_tag="OH499_RS28975"
     CDS             complement(9073..9501)
                     /locus_tag="OH499_RS28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9665..9979)
                     /locus_tag="OH499_RS28980"
     CDS             complement(9665..9979)
                     /locus_tag="OH499_RS28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9967..10245)
                     /locus_tag="OH499_RS28985"
     CDS             complement(9967..10245)
                     /locus_tag="OH499_RS28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(10420..10785)
                     /locus_tag="OH499_RS28990"
     CDS             complement(10420..10785)
                     /locus_tag="OH499_RS28990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(10782..11153)
                     /gene="tolA"
                     /locus_tag="OH499_RS28995"
     CDS             complement(10782..11153)
                     /gene="tolA"
                     /locus_tag="OH499_RS28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(11427..11672)
                     /locus_tag="OH499_RS29000"
     CDS             complement(11427..11672)
                     /locus_tag="OH499_RS29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..8624
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4229..4807)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7574..8301
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tagagatcaa ggggtgaaag tccccgacca ttgaaggacc agcaatccac aaggtccccg
       61 agtcatgcgt tgcataccgc gaggtatggg gcgaagcgtt gacaggggtg ttgacaggcc
      121 agccattgag ccacgaaatg tatattaaat taccgggtgc cgacgttgca ctgttaacgg
      181 aaggcaacat catagggtgc gatactgcga gtgccacatg gacccggcgg ggtctgagac
      241 cctggcatgt caatacgatc tctacgcggg aaccgggaga tctcccctct gaccatctgc
      301 cagtgtcgga gatggcccgc accgggaaga cgaggagtca tagccggtga tgtacggaga
      361 ggagaagtcg gactcgctca tagtagcggc gaagcaggcg aacaacccga aaggagcgga
      421 gtcagtggag cgaaggagcg gggccaaggg gaacgcggaa cagccacaca tgcgccggac
      481 acagagccgg gaaagcatgt cacagaggct gtcacgcgtg cgggaagctg cgaagcagcg
      541 gaagaaagaa cggtttacag cattgttcca cctgctgaca gtcgaagcac tggaagccgc
      601 attcctctcc ctgagcagga aagcggccgc cggagtggat ggcatcaggt ggatggacta
      661 cgccggaaac atgaagaaca acataacaga tctgcaccgg aggctacatc agggcagcta
      721 cagggcgcag cccggcaggc gtcactacat cccaaaagcg gatggaaaac aacgcccgct
      781 cggcatcgcc tcgctggagg acaagatcgt ccagtatgcg ctggtgaaaa tcctgaacgc
      841 agtctatgaa aacgacttta tggggttctc atacgggttc agacccgggc gaagccagca
      901 cgatgcactg gacgcactgg ccacagggct ggtacgcact aacgtaaact gggtactgga
      961 tgccgacatc agtcagttct tcgacagggt gagccacgaa tggctgatca ggttcacaga
     1021 gcatcggatc ggcgaccgga gggtaatcag gctcatacgt aagtggctca cagccgggac
     1081 gtcggaggag ggtcaatggc gagcaacgga ggaaggcacc ccacagggtg cggtcatctc
     1141 accgctgctg gcaaacatat acctccacta cgtcttcgat ctgtgggcgc atcagtggcg
     1201 acgtcgctat gccacaggca atgtggtaat ggtcagatac gccgatgaca tcgtcatcgg
     1261 gttcgacaaa cgatacgatg cccggcgctt ccgtatagcc atgcagcgca gactgaggga
     1321 gttcggactc acggttcacc cggagaaaac ccgtctgatg gagttcggcc gcttcgctgc
     1381 cgaaaaccgt gccatcaggg gaaaaggcaa accagaaacg ttcaacttcc tcgggttcac
     1441 gcacatcagc gggaaagatc gcaacggcag gttcatgctg atacgaaaga cccgccggga
     1501 tcggatgacg gcaactctga aagccatcaa agacggtctg cgaaggcgct ggcattactc
     1561 aatccccgaa cagggaaaat ggctcaggag agtggttcag ggatacctga actatcactc
     1621 ggtaccgggc aacttcccca ccatgcagaa gttcaggaca cacgtaacaa acctctggcg
     1681 ccgggcgctc aggcgcagga gccagaagga tgatacgacc tggacgaaag caaacaaact
     1741 ggcagccgca tggctaccaa gggttcgggt tcttcatcca tggcctgtgg agcggttcac
     1801 cgccagacac ccgaggcagg agcccggtgc gtaaatcgcg cacgccggga tctgtgcggg
     1861 gggtatccgg taacgggtat ccctaccgcg acatgttggc tttaatttat tgttttaaat
     1921 gtcaaagagg aaaacggtat gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg
     1981 ggacataatg gtggtcaggc cagtgggaat gtgaacggta cgtctggtaa aggtggccct
     2041 tcatcaggtg gtggtacgga tccaaacagc gggccgggct ggggtacgac gcatacgccg
     2101 aatggagata ttcataacta taatccgggg gagtttggta acggcgggag taaacccggt
     2161 ggtaatggtg gtaacagcgg caatcatagc ggtagctctg gtggcggaca gtcttcggcc
     2221 actgcgatgg ccttcggtct gcctgccctg gctactccgg gggctgaagg actggcttta
     2281 tcagtttccg gcgatgcgtt gtcggccgcc gttgctgatg tgctggctgc cctgaagggg
     2341 ccgtttaagt ttggtctgtg ggggattgcg atctacggtg tgctgccttc tgagattgca
     2401 aaagatgatc cgaaaatgat gtcaaaaatt atgacgtcat taccggccga tgcggtgacg
     2461 gagactccgg caagtacttt accactggac caggcgacgg ttcgtgtcag acaacgggtt
     2521 gtggatgtgg tgaaggatga gcggcagcat attgcggttg tcgcaggtcg gccaatgagt
     2581 gtccctgtgg tggatgcgaa accgacaaaa cgtccggggg tattcagtgt gtcgattccg
     2641 ggtctcccgt ctctgcaggt gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca
     2701 aaaggcattg ttgctgaaaa aggtgattca cgtccggctg gttttacagc cggtggtaac
     2761 tcccgtgagg ccgttattcg tttcccgaaa gagaccggac agaagccggt ttatgtgtcg
     2821 gtgacagatg ttcttacccc ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc
     2881 cagcaggcat gggacgccgc tcatccggaa gaggggctga aaagagacta tgataaagcg
     2941 aaagctgagc tggatgccga agataaaaat attgcgacct taaacagccg cattgcatcg
     3001 acagagaagg cgctccccgg tgcaagggct gctgttcagg aagccgataa aaaggtaaaa
     3061 gaggcagagg caaataaaga tgattttgtg acttataacc cgcctcatga atatggctcc
     3121 gggtggcagg atcaggttcg ctatcttgat aaggatattc agaatcagaa tgagaaatta
     3181 aaagcggctc aggcatcttt aaacgcaatg aatgaatcct tatccagaga taaggctgca
     3241 cttaccgggg cgatggagag ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat
     3301 aagttaaatg aggaaaagaa caagcctcgc aaggggacta aagactacgg ccatgattat
     3361 catccagtac ctaaaacaga agacataaag ggacttggag agttaaagcg aggtgatcct
     3421 aaaacaccta aacaaggtgg tggtggtaag cgggcccgat ggtatggcga taaaaaacgt
     3481 aaaatttatg aatgggattc ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc
     3541 gaacacctag gcgcatttga tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa
     3601 cggaacatta aaaaatatct ttgagaggta aatatggggc ttaaattacg tttgacatgg
     3661 tttgataaga aaactgaaga gtttaaaggg gaagagtatt ctaaagactt tggtgacgat
     3721 ggttctgtca ttgaaagctt gggaatgcct ttaaaggata atattaataa tggtgagttt
     3781 gatttgaaga aagaatgggt tcctttacta cagccatttt ttaaaaatca aataaaacct
     3841 gatgtggata attgttatat agcttttgat tatcgggacg gtaactggta acaaaaaatt
     3901 atcaaggatg atttgaaata ctgacttccg gtgttcaggg atgaatgccg gagtctttaa
     3961 atacagggaa agtatatgaa aaaggtaaag actatttttc tttttatatt gattgttgct
     4021 ggctttcttc tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg
     4081 gcaccatcgt cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat
     4141 cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct
     4201 ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg
     4261 ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac
     4321 ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg
     4381 ctgctgccag tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga
     4441 taaggcgcag cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac
     4501 gacctacacc gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca
     4561 agggagaacg gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag
     4621 ggagcgacct accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt
     4681 cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt
     4741 gaactctggc aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt
     4801 gcctcacatg ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg
     4861 cagcgcccgc cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat
     4921 ttccccggca cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt
     4981 aagtctttct attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc
     5041 tgaacatttt acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc
     5101 atccccgctg gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc
     5161 caccgacgca cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc
     5221 agaaattcac ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct
     5281 ggacgcacct gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
     5341 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
     5401 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
     5461 cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg
     5521 ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg ggctgcgcat cacgccggta
     5581 aacccgtcgt ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg
     5641 gcatatcccg gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta
     5701 ttacagtctg gccggttttt ttctggttat cggcctgttt ttcggtctgg ctgaactctg
     5761 ggccggccga atgcgttttg tggggctgag ccataaggcg cggcgggagt atgccgatcg
     5821 ctataagcgc gataataacc ggactgtgcc attttcggtg ctggcctcgc tggattcacc
     5881 cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcgtactggc tgaccggcgc
     5941 tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     6001 aaccgtcctg cgcccggacc tctatgcgaa cgcagattat tacctgctgc agccggctaa
     6061 cctgttcggg atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc tggcgcggct
     6121 gctggcgttc ctgctgcttg atggtcattt tgcggccgct gacgaggcgg tgaacgcgaa
     6181 actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgacag acgtccgcca
     6241 cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc
     6301 ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc
     6361 tcgtgatacc tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag
     6421 cgcgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg ctgtggtgta
     6481 cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt
     6541 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     6601 ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt
     6661 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc
     6721 gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgttgccgg aggccctgaa
     6781 aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca
     6841 gacggacagc ggtatcgatg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat
     6901 cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc
     6961 gcaaatcatc attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga
     7021 tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg
     7081 ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt gcgggcagga
     7141 tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta
     7201 tcgcgcggcc agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg agatccgggt
     7261 ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcaca tcagcggcga
     7321 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc
     7381 tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct
     7441 gcgggttgag cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga aaacggaccc
     7501 gctggcggag tacctgcctc cggccgacgg cggcggacca aaagcggatg atgattttgc
     7561 ggggctttac tgatgacact ggagcagtac aacgtcagcc acgcgaagcg gcaggcgcgg
     7621 aacgcggaga aaacacggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg
     7681 gagctggttg cgcgacggct ggggattgcg gcggtacagc cggtttaccg cttcctggac
     7741 agtctggtgg cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc
     7801 agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga cgagccgctg
     7861 acggacgtta ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg
     7921 gccgtgcagt ccctgcgcct ctccatggag ggccgggggg ccaccggctg gcggtacctg
     7981 caccgtatgg ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct ggacggcaga
     8041 acggtggcat ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca ggaagtggtg
     8101 tccgggtatc tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta catctgccct
     8161 gaccggccga cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaat
     8221 ccacagaccg gcgaggcccg caaaacggtg gagctggacc gggagaggct gttcgcgtgc
     8281 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaggac
     8341 gtggaagaca aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg
     8401 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
     8461 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     8521 acgtactttt tcaccggtga aaatccgcaa tgggaacggg acaaacccac aggaatgaag
     8581 ctgtgtgatt acctctatcg aaaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     8641 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgaggt ggtcagcgag
     8701 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     8761 agccgctatt ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg
     8821 tggccgttcg gcaaaaaata acgccagcac cggcatggcc ggtgctgtta ttcttccccg
     8881 agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg ttcgcacatg
     8941 tcggccgggg tatccatgtc cagctcgttc agcttttcca gcaacagcag cgactgatcc
     9001 ttaatgaatt tagccatatt gagggcagtt ttttcctgct tcgtcatgtt ctccattccc
     9061 ttcggtaagt tctcagctaa accagccgga ccagaagcgt tttttcttct gtgtcagttc
     9121 tgttgtcaat ctgtccactt cggcctggag tttccggcgc tcatcatcgt gctggcggcg
     9181 ttcttcttcc cgggatgttt tatcttccag catcagggtg acagcctgcc ggagatcggc
     9241 cagttccttt tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg tattttcacg
     9301 gtgtgaaatg ccgggaattt gtttctcagg gatattcaca tttccgtata cacggatcag
     9361 ttctgacaca tccacgacag ggttgttttt cccgtcacgt gacacggtaa cctttccctg
     9421 ttttacatga ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg cctgtgaaag
     9481 gttgagcaat gttttcgcca ctgtataccc ctgattttat cgggtgtgaa aggtgtgtaa
     9541 tttctcacgg taaatgtatc gctgaatttt acccgttttt ccgttcagga tgtggttttc
     9601 tgacgcgctg cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc cccgtcatcg
     9661 gttattagag gctgtctgcc cccggatacc cggattcttt ctgatgcctg acggtgacga
     9721 tcacaatctg gtccatttcc cggtcgtggc ggtagagcat cacatagcca ctgtcgccaa
     9781 acccgatcac cagttcctta tattccagcg gcagaaacgg caccgggcgg ccaatgtctg
     9841 gcaatgtttt cagttgctgg atagcccgga cgatcacctc acctcctttt ctggctgcca
     9901 gccgattttt ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt gccagcgcgg
     9961 aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc aggtacttat
    10021 ccaggcttcc gcttcttccg cagtaaggtg cagacccgtt tcctgatagt gctgccatgc
    10081 ggcctgtccg tcccgcaggt actggctgcg cttctcttcc cggtcgatgt attcggtgat
    10141 tgcctccagc attagcgcgt gcgctgagcg gtgccggtca tcggccagcg ttttcagccg
    10201 gtccttaagt tcttcatcga gcctgatgga tgttgctgat gacataaaag cccctttgta
    10261 gtcatatgta ttacacatga ctactttaat ccgtggcggc gctatttgcc agaaatacgg
    10321 cttttgctgc ccatttttca gcttcgcttc cactaatcag ggctgctaca ggctcactca
    10381 tcatccagga ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc
    10441 attgagcttt tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa
    10501 ttcaaaattt ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat
    10561 tgccgtcagc gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg
    10621 tccatcgtcc agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc
    10681 ctgtaccggg taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg
    10741 agtgaggaac atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga
    10801 ggggcgtttt tgaatatttg ccaggtattt tcatccggtg ccggcggaat tttggcgtgg
    10861 gtaacggctg atattgctgc tttacagagt ttcggatctc ctccttcagc aatggcgctg
    10921 ttcagagtgc catcacgttg tatggatatc cgcacggtgc acatttttcc cgcataggcc
    10981 tctggttcaa acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta gctactgatt
    11041 ttagatgact tatccagagt ataaattttc ggcggctggg tggtggaatg cttacaggcc
    11101 gtcaggagta aggggctgca caatgccagc aggataaagg gtattaaacg catcgggttg
    11161 atgacctgtc agtaaaaaat gttacatgta tatttgtcac tctttacggc cacgttcatt
    11221 tacataaaca cggcttatcg tatcgttttt acaccaaacg gcccgccatg cagttgcgtg
    11281 cataggcatg cagcgggtta caatttaccg ggcgcgtttc cggcggtttt ccgggtggtt
    11341 tgttgcctgt tttatcccgt agccgccgga aacgccctca gaccgtctga gcggtgcggg
    11401 caatgcgacg ttatggtaaa taccccttac cggcggcgct gtggctgcca ggtgaaggtg
    11461 gtttccgtag cctcgtattt ctcccgacgc atttcctgcg cccgcctggc ttcacgttcg
    11521 tcctgaatgg cacgaagcac cctttcccgc acctgttgtt ttaccgcctt tggcagcgtt
    11581 ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc caggcgcttt
    11641 tcttcagcgg gagtcagttt caggtgcgtc atagtggcct ccgggcgtct caggtttccc
    11701 gaacagttta accgcaggtc ctctcctcat gccagttagc tttcagatgt gtctatacca
    11761 tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat ttgttgccat gagaaatttt
    11821 cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc ctgaagatga
    11881 gaactaatct catatattga cacctgaaaa cgggaggagt aaggtaataa ttatactgtg
    11941 tatatgt
//
