LOCUS       NZ_CP107439            10060 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_17 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP107439
VERSION     NZ_CP107439.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207046
            Assembly: GCF_028222915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:41:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,868
            CDSs (total)                      :: 5,746
            Genes (coding)                    :: 5,587
            CDSs (with protein)               :: 5,587
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 59 of 159
            Pseudo Genes (incomplete)         :: 104 of 159
            Pseudo Genes (internal stop)      :: 19 of 159
            Pseudo Genes (multiple problems)  :: 23 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107439.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_17"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..150
                     /locus_tag="OH508_RS29140"
     CDS             1..150
                     /locus_tag="OH508_RS29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1634..3598
                     /locus_tag="OH508_RS29145"
     CDS             1634..3598
                     /locus_tag="OH508_RS29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3598..4329
                     /gene="mobC"
                     /locus_tag="OH508_RS29150"
     CDS             3598..4329
                     /gene="mobC"
                     /locus_tag="OH508_RS29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4336..4866
                     /locus_tag="OH508_RS29155"
     CDS             4336..4866
                     /locus_tag="OH508_RS29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4893..5072)
                     /locus_tag="OH508_RS29160"
     CDS             complement(4893..5072)
                     /locus_tag="OH508_RS29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5098..5526)
                     /locus_tag="OH508_RS29165"
     CDS             complement(5098..5526)
                     /locus_tag="OH508_RS29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5690..6004)
                     /locus_tag="OH508_RS29170"
     CDS             complement(5690..6004)
                     /locus_tag="OH508_RS29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5992..6270)
                     /locus_tag="OH508_RS29175"
     CDS             complement(5992..6270)
                     /locus_tag="OH508_RS29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6445..6810)
                     /locus_tag="OH508_RS29180"
     CDS             complement(6445..6810)
                     /locus_tag="OH508_RS29180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6807..7178)
                     /gene="tolA"
                     /locus_tag="OH508_RS29185"
     CDS             complement(6807..7178)
                     /gene="tolA"
                     /locus_tag="OH508_RS29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7452..7697)
                     /locus_tag="OH508_RS29190"
     CDS             complement(7452..7697)
                     /locus_tag="OH508_RS29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8024..9709
                     /locus_tag="OH508_RS29195"
     CDS             8024..9709
                     /locus_tag="OH508_RS29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9719..9976
                     /locus_tag="OH508_RS29200"
     CDS             9719..9976
                     /locus_tag="OH508_RS29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             3024..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(254..832)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3599..4326
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgaaaaagg taaagactat ttttcttttt atattgattg ttgctggctt tcttctggcc
       61 gcatgtcagg caaattatat ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc
      121 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
      181 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
      241 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
      301 gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc
      361 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
      421 ttttgcgtgc tttcccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc
      481 gggctgaacg gggagttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc
      541 gagataccag tgtgtgagct atgagaaagc gccacacttc ccgcaaggga gaacggcgga
      601 caggtatccg gtaaacggca gggtcggaac aggagagcgc aagagggagc gacctaccgg
      661 aaacggtggg gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga
      721 gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact ctggcaggag
      781 ggcggagcct atggaaaaac gccaccggcg cggccctgcc gttttgcctc acatgttagt
      841 cccctgctta tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag
      901 tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctgc
      961 aagacggtca ccggacgcgc tgatgtggga tattgtcccc tgattaagtc tttctattcc
     1021 ctgacgatca attatcagga taggtgaacc atgtcggttt ttcgctgaac attttacgtt
     1081 aacctatctc tgttgctttt ttctccttgc ggagaaatgc aagtcatccc cgctggggcg
     1141 tggctgactt caggggaaaa gaccttgctt acgctggctg gacgccaccg acgcacggtc
     1201 ttttcccctc tttctggctc tgtccggctg gacgccggcc acgccagaaa ttcacccggc
     1261 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctgacga
     1321 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
     1381 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
     1441 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
     1501 ctccgcggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     1561 gcatgtccgc ggcttgtgca gcgggggctg cgcatcacgc cggtaaaccc gtcgtctgaa
     1621 ggagagagat tgcatgttta atacggattc gcttgcctgg cagtggcata tcccggagct
     1681 ggcccggcag atttacccgt gggtcagctg gctgtaccgg gagtattaca gtctggccgg
     1741 tttttttctg gttatcggcc tgtttttcgg tctggctgaa ctctgggccg gccgaatgcg
     1801 ttttgtgggg ctgagccata aggcgcggcg ggagtatgcc gatcgctata agcgcgataa
     1861 taaccggact gtgccatttt cggtgctggc ctcgctggat tcacccgggc ggatggtcag
     1921 ggcacggcga catgtctgga actacgcgta ctggctgacc ggcgctgccg gtctggccct
     1981 tctggggggg ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgccc
     2041 ggacctctat gcgaacgcag attattacct gctgcagccg gctaacctgt tcgggatagc
     2101 ctggtgtgcc ggagggcttg tcgccggcgt ccttctggcg cggctgctgg cgttcctgct
     2161 gcttgatggt cattttgcgg ccgctgacga ggcggtgaac gcgaaactga atcaggagag
     2221 ccggcgcagc agccagcgga ccggagaaat gacagacgtc cgccacctgc atttcgggga
     2281 gccggtaccg gttaacgcgc tggccgattt ttccacggag caggcccgga aacagcaggc
     2341 ggtgtttctg ggtaaggatg agcagggcca gccggtgctg gtccctcgtg atacctggcg
     2401 caagaccaat attcagatcc tcggcctgcc cgggagcggc aagagcgcga tggccaccaa
     2461 cgcgctcatt cgctgcgtac gcgatttcgg ggacgctgtg gtgtacttcg accccaaagg
     2521 ggacgcgtgg gcgccgcacg tcttccgggc gcactgcccg gacttcaccc tgctggatct
     2581 gcggccgggg aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgccctgaa
     2641 gaatctgctg gttgccgggt ttaacctgag tgagaccggc gacgtggcgg accactaccg
     2701 catcagcgag cagaaagcgg cgaagctgat tgcggagcag ttcccgcagg gggcaaacat
     2761 tcagcaggtg ctggcggcgg cgtacgcgtt gccggaggcc ctgaaaaagg acgtgaaggg
     2821 gctgatcacc aagctggaga acgtggccga cctgagcgtc ctgcagacgg acagcggtat
     2881 cgatgtggcg gggataatca acggcggcgg ctgcctgtac gtcatcggct cgatggacga
     2941 tgaggcggtg atccgggtgc agaagatgct gtttgcccgc tgcgcgcaaa tcatcattgc
     3001 gcgggatgag ttccggaggt ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct
     3061 gctgtcgaag tacgtgctga acgcgctggg gacgctgcgc agccgggact gcaacctgct
     3121 gctcgcgcac cagtcgctgg gcgacttcgg tcagtgcggg caggatctgc ccgctgattt
     3181 tgtgaaaacc acggtactgg ataacacgcc gatccgctgg ttctatcgcg cggccagcca
     3241 ggagtccgcg cagtgggcgg cgggccagac cggggagatc cgggtggacg tggagcgccg
     3301 ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc ggcgacagct tcatccagaa
     3361 ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac ctgcctgacg ggttcgcggt
     3421 gatgaccggg ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg ttgagcgccg
     3481 ggaaatttcg ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg cggagtacct
     3541 gcctccggcc gacggcggcg gaccaaaagc ggatgatgat tttgcggggc tttactgatg
     3601 acactggagc agtacaacgt cagccacgcg aagcggcagg cgcggaacgc ggagaaaaca
     3661 cggctgaccc tgcgctggct gcgggaagag ctgtgttcca cggcggagct ggttgcgcga
     3721 cggctgggga ttgcggcggt acagccggtt taccgcttcc tggacagtct ggtggcgaaa
     3781 gggctgctgg tccgggcgaa atacccggtt gacggccgtc aggtcagcgt gtgggggctg
     3841 acgccgcacg gggtggcgtt ctcgttcgac gaggacgagc cgctgacgga cgttattccg
     3901 tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg
     3961 cgcctctcca tggagggccg gggggccacc ggctggcggt acctgcaccg tatggccctg
     4021 aaagggatga aagtgccgga cgcgctggcg gagctggacg gcagaacggt ggcatttgag
     4081 gtagagcgca ccgtcaagag ccgcaggcgg tatcaggaag tggtgtccgg gtatctgttc
     4141 aaccgccggg cgaacggcat cgatgaaatc tggtacatct gccctgaccg gccgacgctt
     4201 gtccgggtac agcgggcgat tctggcggtg gatgaaatcg tgaatccaca gaccggcgag
     4261 gcccgcaaaa cggtggagct ggaccgggag aggctgttcg cgtgctttaa atttatgaca
     4321 acggagtgag ggattatggc gctgtacatg gtatttattg aggacgtgga agacaaagac
     4381 ggcgagcgct gcattatgcc aaccagcgag catctggaag acatggtggt gctcgcctgg
     4441 cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa
     4501 tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc
     4561 ggtgaaaatc cgcaatggga acgggacaaa cccacaggaa tgaagctgtg tgattacctc
     4621 tatcgaaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag
     4681 tgctcaccgg agcagattaa cgcgatgatt gaggtggtca gcgagcggca cggggaaagc
     4741 gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac
     4801 gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa
     4861 aaataacgcc agcaccggca tggccggtgc tgttattctt ccccgagctt atcccgcatc
     4921 acctgccaga gtgtttccgc atcctcgtga agccgttcgc acatgtcggc cggggtatcc
     4981 atgtccagct cgttcagctt ttccagcaac agcagcgact gatccttaat gaatttagcc
     5041 atattgaggg cagttttttc ctgcttcgtc atgttctcca ttcccttcgg taagttctca
     5101 gctaaaccag ccggaccaga agcgtttttt cttctgtgtc agttctgttg tcaatctgtc
     5161 cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg cggcgttctt cttcccggga
     5221 tgttttatct tccagcatca gggtgacagc ctgccggaga tcggccagtt ccttttgcat
     5281 tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt tcacggtgtg aaatgccggg
     5341 aatttgtttc tcagggatat tcacatttcc gtatacacgg atcagttctg acacatccac
     5401 gacagggttg tttttcccgt cacgtgacac ggtaaccttt ccctgtttta catgattgta
     5461 tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt gaaaggttga gcaatgtttt
     5521 cgccactgta tacccctgat tttatcgggt gtgaaaggtg tgtaatttct cacggtaaat
     5581 gtatcgctga attttacccg tttttccgtt caggatgtgg ttttctgacg cgctgcgctt
     5641 gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt catcggttat tagaggctgt
     5701 ctgcccccgg atacccggat tctttctgat gcctgacggt gacgatcaca atctggtcca
     5761 tttcccggtc gtggcggtag agcatcacat agccactgtc gccaaacccg atcaccagtt
     5821 ccttatattc cagcggcaga aacggcaccg ggcggccaat gtctggcaat gttttcagtt
     5881 gctggatagc ccggacgatc acctcacctc cttttctggc tgccagccga tttttggttt
     5941 tgagaaagtc ctgaaggcgt tgtaaatcgc gctgtgccag cgcggaaatc gttacctgtg
     6001 gcatggcgga gcatcctgct cgttctcagt tccccaggta cttatccagg cttccgcttc
     6061 ttccgcagta aggtgcagac ccgtttcctg atagtgctgc catgcggcct gtccgtcccg
     6121 caggtactgg ctgcgcttct cttcccggtc gatgtattcg gtgattgcct ccagcattag
     6181 cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc agccggtcct taagttcttc
     6241 atcgagcctg atggatgttg ctgatgacat aaaagcccct ttgtagtcat atgtattaca
     6301 catgactact ttaatccgtg gcggcgctat ttgccagaaa tacggctttt gctgcccatt
     6361 tttcagcttc gcttccacta atcagggctg ctacaggctc actcatcatc caggactatt
     6421 gcagcctgcg ggttaaagga cctgttactg gcggcaacca tcatcattga gcttttgggt
     6481 ggtgactggt cgcggatcct gtaagccgtc atttttcaga ctgaattcaa aatttataac
     6541 ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc ctcattgccg tcagcgtttc
     6601 ccgaaaaaaa aggtgacagc cggtaagcga cacgttgtcc actcgtccat cgtccagaat
     6661 atcaaacgtg gcggtcacct gaccttcgat gcgttttgct gcggcctgta ccgggtaggt
     6721 tggctgcagt gtggttacgg gttttagtgg tatcgcatgt accggagtga ggaacatggc
     6781 cgccagcact gtcagagtaa aagctttcat ggtctgaaat ccagaggggc gtttttgaat
     6841 atttgccagg tattttcatc cggtgccggc ggaattttgg cgtgggtaac ggctgatatt
     6901 gctgctttac agagtttcgg atctcctcct tcagcaatgg cgctgttcag agtgccatca
     6961 cgttgtatgg atatccgcac ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc
     7021 tgcatttcaa cagcggtctt gatggccgca gcgtagctac tgattttaga tgacttatcc
     7081 agagtataaa ttttcggcgg ctgggtggtg gaatgcttac aggccgtcag gagtaagggg
     7141 ctgcacaatg ccagcaggat aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa
     7201 aaaatgttac atgtatattt gtcactcttt acggccacgt tcatttacat aaacacggct
     7261 tatcgtatcg tttttacacc aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg
     7321 ggttacaatt taccgggcgc gtttccggcg gttttccggg tggtttgttg cctgttttat
     7381 cccgtagccg ccggaaacgc cctcagaccg tctgagcggt gcgggcaatg cgacgttatg
     7441 gtaaataccc cttaccggcg gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg
     7501 tatttctccc gacgcatttc ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga
     7561 agcacccttt cccgcacctg ttgttttacc gcctttggca gcgttttgtt ccctttcagg
     7621 cccatggcca gtgcctgagc gacaaaggcg tccgccaggc gcttttcttc agcgggagtc
     7681 agtttcaggt gcgtcatagt ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc
     7741 aggtcctctc ctcatgccag ttagctttca gatgtgtcta taccatctga aatgtaagtg
     7801 tgatgaatgt cttaaataat aaaatttgtt gccatgagaa attttcggga aaagacgcga
     7861 tctgcttaca ggtggctccg ggcgcttttt tgtgcctgaa gatgagaact aatctcatat
     7921 attgacacct gaaaacggga ggagtaaggt aataattata ctgtgtatat gtacagtatg
     7981 ttggctttaa tttattgttt taaatgtcaa agaggaaaac ggtatgagtg gtggagacgg
     8041 tcgaggtccg ggtaattcag gtctgggaca taatggtggt caggccagtg ggaatgtgaa
     8101 cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt acggatccaa acagcgggcc
     8161 gggctggggt acgacgcata cgccgaatgg agatattcat aactataatc cgggggagtt
     8221 tggtaacggc gggagtaaac ccggtggtaa tggtggtaac agcggcaatc atagcggtag
     8281 ctctggtggc ggacagtctt cggccactgc gatggccttc ggtctgcctg ccctggctac
     8341 tccgggggct gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc
     8401 tgatgtgctg gctgccctga aggggccgtt taagtttggt ctgtggggga ttgcgatcta
     8461 cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac
     8521 gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac tggaccaggc
     8581 gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc agcatattgc
     8641 ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc
     8701 gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg taccgaaagg
     8761 tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc
     8821 ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc cgaaagagac
     8881 cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac aggtaaaaca
     8941 gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc cggaagaggg
     9001 gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata aaaatattgc
     9061 gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa gggctgctgt
     9121 tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt ttgtgactta
     9181 taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc ttgataagga
     9241 tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg caatgaatga
     9301 atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga aacaaaagga
     9361 gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg
     9421 gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca taaagggact
     9481 tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc
     9541 ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc acggtgagct
     9601 tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca aaacgggtaa
     9661 gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag aggtaaatat
     9721 ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta aaggggaaga
     9781 gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa tgcctttaaa
     9841 ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt tactacagcc
     9901 attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt ttgattatcg
     9961 ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac ttccggtgtt
    10021 cagggatgaa tgccggagtc tttaaataca gggaaagtat
//
