LOCUS       NZ_CP107431             5596 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_19 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP107431
VERSION     NZ_CP107431.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207048
            Assembly: GCF_028222115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:27:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,782
            Genes (coding)                    :: 5,634
            CDSs (with protein)               :: 5,634
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 56 of 148
            Pseudo Genes (incomplete)         :: 97 of 148
            Pseudo Genes (internal stop)      :: 18 of 148
            Pseudo Genes (multiple problems)  :: 23 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_19"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..213
                     /locus_tag="OH521_RS29405"
     CDS             1..213
                     /locus_tag="OH521_RS29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(589..717)
                     /locus_tag="OH521_RS29410"
     CDS             complement(589..717)
                     /locus_tag="OH521_RS29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            880..1104
                     /locus_tag="OH521_RS29415"
     CDS             880..1104
                     /locus_tag="OH521_RS29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1088..1354
                     /locus_tag="OH521_RS29420"
     CDS             1088..1354
                     /locus_tag="OH521_RS29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1424..1774)
                     /locus_tag="OH521_RS29425"
     CDS             complement(1424..1774)
                     /locus_tag="OH521_RS29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1840..2385)
                     /locus_tag="OH521_RS29430"
     CDS             complement(1840..2385)
                     /locus_tag="OH521_RS29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2411..2737)
                     /locus_tag="OH521_RS29435"
     CDS             complement(2411..2737)
                     /locus_tag="OH521_RS29435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3095..3253)
                     /locus_tag="OH521_RS29540"
     CDS             complement(3095..3253)
                     /locus_tag="OH521_RS29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3893..4069
                     /locus_tag="OH521_RS29440"
     CDS             3893..4069
                     /locus_tag="OH521_RS29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4059..4403
                     /locus_tag="OH521_RS29445"
     CDS             4059..4403
                     /locus_tag="OH521_RS29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4612..>5046
                     /locus_tag="OH521_RS29450"
                     /pseudo=""
     CDS             4612..>5046
                     /locus_tag="OH521_RS29450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5081..5590
                     /locus_tag="OH521_RS29455"
     CDS             5081..5590
                     /locus_tag="OH521_RS29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     Typing          3728..3807
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3145..3542)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4394..5538
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.738"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atgaccgagc tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg
       61 aaaaggctgg acgagtggga gagcgccttc ggggaatggc ggaaaatgtc agggcttatg
      121 caacgggaga acgcggcgct gagcgagcgc gtgacgcgct tgagtcagca ggtggaacgc
      181 ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg tcatgagatg gccgtggccg
      241 cagaacgggc gcacgagcaa cggcagcacg aaaaggaact gacggaagcg cgttcccggc
      301 agcgaagcta tgacggtccg tcgctgggct gaaatgcagg aaaacagggt tctcatcggg
      361 catttacgct gaaaccggat gtcattcacc tggctgcatg gctatgcagc caggtaaaat
      421 ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt gccggtttta cctgactgcc
      481 accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag
      541 cgtgaacggc agatacaaaa aagccccgga gggcttttaa ctactgactt atttttttga
      601 agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt tttttattgt cacgctggcg
      661 tttatcaggt tcccctgttg aatcggcaat tcgtttcgga cccggcttaa gacccataat
      721 cgttatcctt tcaatagtta agaggaaagc cctcaagcta gatatattga ccgcgttacg
      781 gattatcaag aggggctaac aagattattt tttgttcttt taggttgatt gtggtactgt
      841 tgatatacat gtatagcaaa tggtgttcgg gaggtttata tgcttgctat ccggttatct
      901 gatgagattg agtcccgtct ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt
      961 tatgcgcggg aagcaatact ggcgcatctg gaagacctgg aggattatta tctttcagca
     1021 gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc attcgtctga agacgtgagg
     1081 aagtcacttg gtctggacga ttaactattc cgatcgggcg ctcaaatcgt tacgcaagat
     1141 ggacaaacag aacgcacgac ggattgtgga ttttatgagt ttacgcattg cagttgctgc
     1201 cgatcctcgc cagtcaggga agccgctcaa aggtgagctg ggcgagttct ggcgctatcg
     1261 ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac gagcttgtta tccttgccgc
     1321 cacgattgga catcgccgcg aagtttacga ctgaagcccg ctatgcgggc ttttttgatg
     1381 ccgacaacag gtattatggt aaatactcga ctaactacgt gttctactgt ttgtcggttt
     1441 tagttaacca gcaggttgtc ttaacgataa tgtagccgtt atttttaact aataaggtca
     1501 tattgttaac gtcaggcata ataaaagaca ttatggcctc agagttatca tcagaagcag
     1561 ctctctgcat tactacttcg attccctttt ctttgaatct ataagtatcc agttgcctat
     1621 taaacttaga aagacctaca accatactta gtttgttatc tttaacagta agagatgctt
     1681 cctgagtacc attacatcgg tagtaagcgg tgtaaccgtc agcaaaagat cgaccgggga
     1741 atataaatgc taggaacgca attaaccaat acatagttga gaatcttggt attacacatt
     1801 tcactatcct ctctttcatt tttcgttcac catactttgt tatctgattt ttaatttttc
     1861 tattttataa accaagtctc taccaaaaag gcgaaaatta tcctcaaata ataataattg
     1921 tttaagaaca tccatgaaag cagctgcttc accctcagtt atgggagcta ctctatataa
     1981 catataacct gagttaaggg tttcttttat actttcttca atgctcatta taaatattag
     2041 tggattatca tttttcttcc cccatgggtt tctaattaat ccttcgatgt actcaggtcc
     2101 tttgcgtcct attgcctgcg caatattttt tatttcatct gcgtgagggc tttgttgtac
     2161 agctttgttt atgcctgctt gagtgatttt cagagactct acaccgtgta tgttgtatga
     2221 gataaaatgt aataaattaa gatgcaatgc aattaatcca tttttgttta catctgagta
     2281 tttggataaa aaacgtaatg aagtgttaac tctcccttga agcctaacaa ttgggagtgc
     2341 tgccttgtat atttccttct ccgttaaata ccgtttaaaa aacataattc tccgcctccg
     2401 tatagcgtgc ctattttatt tcgtcgtaaa acttagctct gaaatattcc tttagaacca
     2461 tcatgtcatt aataattgaa tctatctcat ccattttttc ttggtattct tttaatggtg
     2521 aagggagcgg taactcaata tttgaaaagt gattggcgag atagggatca aggtttctca
     2581 tccattcagg cttttcgcca atgcgttctt caactgaaag tgaacctgat aatctctggt
     2641 taataagatc acttagactc attttggttt tattcgaaag ccgttgtaag gattgcttta
     2701 atcctatatc tagcatgata tttgttcttt ctctcattac ccccccttgc tattgcgttt
     2761 tttgttgcgc acaaagctac cattgcgcat taagaaacgc aataaaaaag gagggaggga
     2821 gtggtttgta catgatgtgc agtgcgctga gggcatcagt ggcgggtgtt tttccgtggg
     2881 atggatggag ctccggggat aacagaactg acgatcattt aaaggatctt cttgagatcc
     2941 tttttttctg cgcgtaatct cttgcactgt aaacgaaaaa accgcctggg gaggcggttt
     3001 gatcgaaggt taagtcagtt ggggaactgc ttaaccgggt aactgggtat acgagaccgc
     3061 agataccaaa actgttcttt cagtgtagcc gcagttaggc acccacttca agacctctca
     3121 atatctctct cgtatatatc cagttaccaa tggctgctgc cagtggcgtt tttgcgtgtc
     3181 cgtccgggtt ggactcaaga tgatagttac cggaaagggc gcagcagtcg ggctgaacgg
     3241 ggggttcgtg catacagccc agcttggagc gaacgaccta aaccgagccg agataccagc
     3301 agcgtgagct atgagaaagc gccacgcttc ccgaaaggga aaaaggcgga acaggtatcc
     3361 ggtaaacggc agggtcggaa caggagagcg cacgagggag ccgccggaga gaaatcaccg
     3421 gcatctttaa gtcctgtcgg gtttcgtcct cctctgactt gagcgtccat ttctgtgatg
     3481 ctcgtcaggg gaggcggagc ctatggaaaa acggcaacgc cgcatccttt tctccggccc
     3541 tttctgattt gagcgccttt gctttatccc gttatccgtg agtcggtcac gcccgccgca
     3601 gccgaacggc aggagcgcag ctaccgagtg agcgaggagg cgtcatttca tctgtccgtg
     3661 catggtattg cggacgctcc tgagccacat atcacacctg ccacatgagg cactttcatg
     3721 aatcgtgctc tcatgccaac atagtaagcc agtatacaca gcgtaaacgc tgtgactggt
     3781 tcagggctgc gccccgaaac ccgctaacac cactgacgcg ctggcgcttg tccaacgccg
     3841 gagcgacccg gaaaactttt ccgtgccgtc cggcgtttgc gaaggaggga taatgcagaa
     3901 gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg catccggcag tgatggcaaa
     3961 tctgatgcgt caggcacagg aggaagatta ccgccgggag cagatggcag agcttgagcg
     4021 ttcagcccgg tcacattttg cggatatcgg aggagaggat ggcggttaag cgtacccgtt
     4081 ttttaggtat ccgggtgacc gatggggagt accagcagct gctggagcgc tgcaatggaa
     4141 ggcaactggc ggtgtggatg cgggaaacct gtctcgatac gcgcccggcg cggtcgttgc
     4201 ggctgccgtc catcgatccg gttttgctgc gccagcttgc cgggatgggg aacaacctca
     4261 accagatcgc ccggaaaatt aacggcggcc agtggtcagg cgctgaccgg gttcaggtgg
     4321 tggccgcgct gatggccatt gatgccgaac tcgagcggct gcggcatgcc gtgctggaaa
     4381 agggggcaga cgatgatcgt taagtttcat ccgcgagggc gaggcggcgg tgccgggccg
     4441 gtggattatc tgctggggaa agaccgccag cgcgacgggg ccatcgtcct gcagggaaag
     4501 ccggaggagg tccgggagct tatcgacgcc tcgccctacg ccaaaaagta cacctccggc
     4561 gtcctgtctt ttgctgaaca ggatttaccg cccggccagc gtgaaaagct gatggcgagc
     4621 ttcgagcggg ttctgatgcc cggactcgat aaagaccagt acagcgtgct gtgggttgaa
     4681 caccgggaca aggggcggct ggaactgaat tttctggtac cgaacacgga actgctgacc
     4741 ggcaggcgtc tccagccgta ctacgaccgg gcagaccgtc cgcgcatcga tgcgtggcag
     4801 accgtggtga acgggcgact ggggctgcac gacccgaacg caccggagaa ccggcgcgcg
     4861 ctggtgacgc catcttcgtt acccaaaacg aagcaggaag ccgcagaggc tattacgcgg
     4921 ggcttactgg ccctcgcctc gtcaggggag cttaaaacgc gtcaggacgt cactgaggcg
     4981 ctggaaagcg caggttttga ggtcgtgcgc accacaaaga gcagcatcag cattgccgac
     5041 ccggacggag ggcgaaacat ccgacttaag ggagccatct atgaacagtc ttttaacgct
     5101 ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag agtaccggcg aaatgctgaa
     5161 agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa gcgggaagag
     5221 aatcagcgcc gccatccgcg cccacgagca gggtatgagc gcggccatgc agtcgaaccg
     5281 cctgagcgtg atgcgtatgg tcgggcggac atggctgacc atcacgatgg tgtcaggact
     5341 gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg agcttgatag cgtcaaatct
     5401 ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg aacgcgaaga cctggggcgt
     5461 gacgtacctg gaagacagca acggacgttt tctggtgctg ccgaagggga cggccgtaga
     5521 caggacgcag agctggacgg tcgggaacgg gcggtcgaag cagaacgcgc tgagactggt
     5581 gaaggagtaa gtgccg
//
