LOCUS       NZ_CP107424            10060 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_20 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP107424
VERSION     NZ_CP107424.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207049
            Assembly: GCF_028221855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:25:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,603
            CDSs (total)                      :: 5,481
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 45 of 127
            Pseudo Genes (incomplete)         :: 85 of 127
            Pseudo Genes (internal stop)      :: 13 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107424.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_20"
                     /isolation_source="lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..1965
                     /locus_tag="OH530_RS27825"
     CDS             1..1965
                     /locus_tag="OH530_RS27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1965..2696
                     /gene="mobC"
                     /locus_tag="OH530_RS27830"
     CDS             1965..2696
                     /gene="mobC"
                     /locus_tag="OH530_RS27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2703..3233
                     /locus_tag="OH530_RS27835"
     CDS             2703..3233
                     /locus_tag="OH530_RS27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3260..3439)
                     /locus_tag="OH530_RS27840"
     CDS             complement(3260..3439)
                     /locus_tag="OH530_RS27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3465..3893)
                     /locus_tag="OH530_RS27845"
     CDS             complement(3465..3893)
                     /locus_tag="OH530_RS27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4057..4371)
                     /locus_tag="OH530_RS27850"
     CDS             complement(4057..4371)
                     /locus_tag="OH530_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4359..4637)
                     /locus_tag="OH530_RS27855"
     CDS             complement(4359..4637)
                     /locus_tag="OH530_RS27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4812..5177)
                     /locus_tag="OH530_RS27860"
     CDS             complement(4812..5177)
                     /locus_tag="OH530_RS27860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH530_RS27865"
     CDS             complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH530_RS27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5819..6064)
                     /locus_tag="OH530_RS27870"
     CDS             complement(5819..6064)
                     /locus_tag="OH530_RS27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6391..8076
                     /locus_tag="OH530_RS27875"
     CDS             6391..8076
                     /locus_tag="OH530_RS27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8086..8343
                     /locus_tag="OH530_RS27880"
     CDS             8086..8343
                     /locus_tag="OH530_RS27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            8428..8577
                     /locus_tag="OH530_RS27885"
     CDS             8428..8577
                     /locus_tag="OH530_RS27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1391..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8681..9259)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1966..2693
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt
       61 tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt ttttctggtt
      121 atcggcctgt ttttcggtct ggctgaactc tgggccggcc gaatgcgttt tgtggggctg
      181 agccataagg cgcggcggga gtatgccgat cgctataagc gcgataataa ccggactgtg
      241 ccattttcgg tgctggcctc gctggattca cccgggcgga tggtcagggc acggcgacat
      301 gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg
      361 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg
      421 aacgcagatt attacctgct gcagccggct aacctgttcg ggatagcctg gtgtgccgga
      481 gggcttgtcg ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat
      541 tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc
      601 cagcggaccg gagaaatgac agacgtccgc cacctgcatt tcggggagcc ggtaccggtt
      661 aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt gtttctgggt
      721 aaggatgagc agggccagcc ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt
      781 cagatcctcg gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc
      841 tgcgtacgcg atttcgggga cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg
      901 ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa
      961 ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt
     1021 gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag
     1081 aaagcggcga agctgattgc ggagcagttc ccgcaggggg caaacattca gcaggtgctg
     1141 gcggcggcgt acgcgttgcc ggaggccctg aaaaaggacg tgaaggggct gatcaccaag
     1201 ctggagaacg tggccgacct gagcgtcctg cagacggaca gcggtatcga tgtggcgggg
     1261 ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga ggcggtgatc
     1321 cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc
     1381 cggaggtggc cgcacgcgag catcatgctc gatgagatta aatatctgct gtcgaagtac
     1441 gtgctgaacg cgctggggac gctgcgcagc cgggactgca acctgctgct cgcgcaccag
     1501 tcgctgggcg acttcggtca gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg
     1561 gtactggata acacgccgat ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag
     1621 tgggcggcgg gccagaccgg ggagatccgg gtggacgtgg agcgccgccg ggccagccgg
     1681 gaggcgggga acgtggagca catcagcggc gacagcttca tccagaagga ggcgcggccg
     1741 ctgtttgacg tgaacaccct gcaacacctg cctgacgggt tcgcggtgat gaccgggctg
     1801 ggcgtggcgc ggctggcgtt cagcagcccg ctgcgggttg agcgccggga aatttcgctg
     1861 aaaaccttcc cggtgctggc gaaaacggac ccgctggcgg agtacctgcc tccggccgac
     1921 ggcggcggac caaaagcgga tgatgatttt gcggggcttt actgatgaca ctggagcagt
     1981 acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc
     2041 gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg
     2101 cggcggtaca gccggtttac cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc
     2161 gggcgaaata cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg
     2221 tggcgttctc gttcgacgag gacgagccgc tgacggacgt tattccgttt cagccgtcaa
     2281 gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg
     2341 agggccgggg ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag
     2401 tgccggacgc gctggcggag ctggacggca gaacggtggc atttgaggta gagcgcaccg
     2461 tcaagagccg caggcggtat caggaagtgg tgtccgggta tctgttcaac cgccgggcga
     2521 acggcatcga tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc
     2581 gggcgattct ggcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg
     2641 tggagctgga ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga
     2701 ttatggcgct gtacatggta tttattgagg acgtggaaga caaagacggc gagcgctgca
     2761 ttatgccaac cagcgagcat ctggaagaca tggtggtgct cgcctggcag accggggcgt
     2821 atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt
     2881 atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc
     2941 aatgggaacg ggacaaaccc acaggaatga agctgtgtga ttacctctat cgaaaggtgg
     3001 agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc
     3061 agattaacgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga
     3121 agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtgcctg
     3181 aactgccgga ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa taacgccagc
     3241 accggcatgg ccggtgctgt tattcttccc cgagcttatc ccgcatcacc tgccagagtg
     3301 tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg tccagctcgt
     3361 tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata ttgagggcag
     3421 ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct aaaccagccg
     3481 gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac ttcggcctgg
     3541 agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt tttatcttcc
     3601 agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc cagaagctgt
     3661 tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat ttgtttctca
     3721 gggatattca catttccgta tacacggatc agttctgaca catccacgac agggttgttt
     3781 ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag cgttcttctg
     3841 gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc cactgtatac
     3901 ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta tcgctgaatt
     3961 ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc aaaccggcga
     4021 cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg cccccggata
     4081 cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt cccggtcgtg
     4141 gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct tatattccag
     4201 cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct ggatagcccg
     4261 gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga gaaagtcctg
     4321 aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca tggcggagca
     4381 tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc cgcagtaagg
     4441 tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag gtactggctg
     4501 cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc gtgcgctgag
     4561 cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc gagcctgatg
     4621 gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat gactacttta
     4681 atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt cagcttcgct
     4741 tccactaatc agggctgcta caggctcact catcatccag gactattgca gcctgcgggt
     4801 taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt gactggtcgc
     4861 ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc
     4921 gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagg
     4981 tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc aaacgtggcg
     5041 gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg ctgcagtgtg
     5101 gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc cagcactgtc
     5161 agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt tgccaggtat
     5221 tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct gctttacaga
     5281 gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt tgtatggata
     5341 tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc atttcaacag
     5401 cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga gtataaattt
     5461 tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg cacaatgcca
     5521 gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa atgttacatg
     5581 tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat cgtatcgttt
     5641 ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt tacaatttac
     5701 cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg
     5761 gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta aatacccctt
     5821 accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac
     5881 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     5941 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     6001 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     6061 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     6121 atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt
     6181 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt
     6241 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     6301 aacgggagga gtaaggtaat aattatactg tgtatatgta cagtatgttg gctttaattt
     6361 attgttttaa atgtcaaaga ggaaaacggt atgagtggtg gagacggtcg aggtccgggt
     6421 aattcaggtc tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt
     6481 aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg
     6541 acgcatacgc cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg
     6601 agtaaacccg gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggcgga
     6661 cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc tggctactcc gggggctgaa
     6721 ggactggctt tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga tgtgctggct
     6781 gccctgaagg ggccgtttaa gtttggtctg tgggggattg cgatctacgg tgtgctgcct
     6841 tctgagattg caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc
     6901 gatgcggtga cggagactcc ggcaagtact ttaccactgg accaggcgac ggttcgtgtc
     6961 agacaacggg ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcaggt
     7021 cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt
     7081 gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt tccgacagcg
     7141 aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttaca
     7201 gccggtggta actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg
     7261 gtttatgtgt cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa
     7321 gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagac
     7381 tatgataaag cgaaagctga gctggatgcc gaagataaaa atattgcgac cttaaacagc
     7441 cgcattgcat cgacagagaa ggcgctcccc ggtgcaaggg ctgctgttca ggaagccgat
     7501 aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat
     7561 gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag
     7621 aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc cttatccaga
     7681 gataaggctg cacttaccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag
     7741 gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc gcaaggggac taaagactac
     7801 ggccatgatt atcatccagt acctaaaaca gaagacataa agggacttgg agagttaaag
     7861 cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta agcgggcccg atggtatggc
     7921 gataaaaaac gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtatcgt
     7981 gccagtgatg gcgaacacct aggcgcattt gatcccaaaa cgggtaagca ggttaaaggg
     8041 ccagatccaa aacggaacat taaaaaatat ctttgagagg taaatatggg gcttaaatta
     8101 cgtttgacat ggtttgataa gaaaactgaa gagtttaaag gggaagagta ttctaaagac
     8161 tttggtgacg atggttctgt cattgaaagc ttgggaatgc ctttaaagga taatattaat
     8221 aatggtgagt ttgatttgaa gaaagaatgg gttcctttac tacagccatt ttttaaaaat
     8281 caaataaaac ctgatgtgga taattgttat atagcttttg attatcggga cggtaactgg
     8341 taacaaaaaa ttatcaagga tgatttgaaa tactgacttc cggtgttcag ggatgaatgc
     8401 cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa agactatttt tctttttata
     8461 ttgattgttg ctggctttct tctggccgca tgtcaggcaa attatatccg ggatgttcag
     8521 ggcggaacgg tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa
     8581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg
     8641 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     8701 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     8761 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     8821 agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt cccgggttgg actcaagtga
     8881 acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct tacagcccag
     8941 cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg agaaagcgcc
     9001 acacttcccg caagggagaa cggcggacag gtatccggta aacggcaggg tcggaacagg
     9061 agagcgcaag agggagcgac ctaccggaaa cggtggggat ctttaagtcc tgtcgggttt
     9121 cgcccgtact gtcagattca tggttgagcc tcacggctcc cacagatgca ccgaaaaagc
     9181 gtctgtttat gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg
     9241 ccctgccgtt ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt
     9301 tgtatgtgtc cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagcg
     9361 aggaggcgca atttccccgg cacctgcaag acggtcaccg gacgcgctga tgtgggatat
     9421 tgtcccctga ttaagtcttt ctattccctg acgatcaatt atcaggatag gtgaaccatg
     9481 tcggtttttc gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga
     9541 gaaatgcaag tcatccccgc tggggcgtgg ctgacttcag gggaaaagac cttgcttacg
     9601 ctggctggac gccaccgacg cacggtcttt tcccctcttt ctggctctgt ccggctggac
     9661 gccggccacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa
     9721 atccacggtg ctggacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt
     9781 cagccgctcg ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg
     9841 gtgccgcctt ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt
     9901 ttccgcatgg ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca atcaccacgg
     9961 acgtgaccgc cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcgc
    10021 atcacgccgg taaacccgtc gtctgaagga gagagattgc
//
