LOCUS       NZ_CP107378             5596 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_31 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP107378
VERSION     NZ_CP107378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207060
            Assembly: GCF_028220985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:44:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,016
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,713
            CDSs (with protein)               :: 5,713
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 69 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 25 of 181
            Pseudo Genes (multiple problems)  :: 33 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_31"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            join(5263..5596,1..11)
                     /locus_tag="OH494_RS29970"
     CDS             join(5263..5596,1..11)
                     /locus_tag="OH494_RS29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>654
                     /locus_tag="OH494_RS29975"
                     /pseudo=""
     CDS             220..>654
                     /locus_tag="OH494_RS29975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1198
                     /locus_tag="OH494_RS29980"
     CDS             689..1198
                     /locus_tag="OH494_RS29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1205..1417
                     /locus_tag="OH494_RS29985"
     CDS             1205..1417
                     /locus_tag="OH494_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2084..2308
                     /locus_tag="OH494_RS29990"
     CDS             2084..2308
                     /locus_tag="OH494_RS29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2292..2558
                     /locus_tag="OH494_RS29995"
     CDS             2292..2558
                     /locus_tag="OH494_RS29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2628..2978)
                     /locus_tag="OH494_RS30000"
     CDS             complement(2628..2978)
                     /locus_tag="OH494_RS30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3044..3589)
                     /locus_tag="OH494_RS30005"
     CDS             complement(3044..3589)
                     /locus_tag="OH494_RS30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3615..3941)
                     /locus_tag="OH494_RS30010"
     CDS             complement(3615..3941)
                     /locus_tag="OH494_RS30010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4299..4457)
                     /locus_tag="OH494_RS30110"
     CDS             complement(4299..4457)
                     /locus_tag="OH494_RS30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5097..5273
                     /locus_tag="OH494_RS30015"
     CDS             5097..5273
                     /locus_tag="OH494_RS30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          4932..5011
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4349..4746)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2..1533
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatcgtta agtttcatcc gcgagggcga ggcggcggtg ccgggccggt ggattatctg
       61 ctggggaaag accgccagcg cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc
      121 cgggagctta tcgacgcctc gccctacgcc aaaaagtaca cctccggcgt cctgtctttt
      181 gctgaacagg atttaccgcc cggccagcgt gaaaagctga tggcgagctt cgagcgggtt
      241 ctgatgcccg gactcgataa agaccagtac agcgtgctgt gggttgaaca ccgggacaag
      301 gggcggctgg aactgaattt tctggtaccg aacacggaac tgctgaccgg caggcgtctc
      361 cagccgtact acgaccgggc agaccgtccg cgcatcgatg cgtggcagac cgtggtgaac
      421 gggcgactgg ggctgcacga cccgaacgca ccggagaacc ggcgcgcgct ggtgacgcca
      481 tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta ttacgcgggg cttactggcc
      541 ctcgcctcgt caggggagct taaaacgcgt caggacgtca ctgaggcgct ggaaagcgca
      601 ggttttgagg tcgtgcgcac cacaaagagc agcatcagca ttgccgaccc ggacggaggg
      661 cgaaacatcc gacttaaggg agccatctat gaacagtctt ttaacgctgg cgaaggactt
      721 agagcagaaa tcgaaagcgc agcagcagag taccggcgaa atgctgaaag ccgcattcag
      781 cgagcacgag aagtctgtca gagcggaact gagcgaaagc gggaagagaa tcagcgccgc
      841 catccgcgcc cacgagcagg gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat
      901 gcgtatggtc gggcggacat ggctgaccat cacgatggtg tcaggactgc tgttcgccag
      961 cctgagcggg gtgttgtggt atcaggggag cttgatagcg tcaaatctgg ccgaaatcga
     1021 ccggcagaac gcagcgctgt cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga
     1081 agacagcaac ggacgttttc tggtgctgcc gaaggggacg gccgtagaca ggacgcagag
     1141 ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg agactggtga aggagtaagt
     1201 gccgatgacc gagctggaaa aacagttgct gagcgcatta gagcagctac agcaggacta
     1261 ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa tggcggaaaa tgtcagggct
     1321 tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga
     1381 acgcttgagc gggcaactgc agcgtttgag ccggtagtgc agcgtcatga gatggccgtg
     1441 gccgcagaac gggcgcacga gcaacggcag cacgaaaagg aactgacgga agcgcgttcc
     1501 cggcagcgaa gctatgacgg tccgtcgctg ggctgaaatg caggaaaaca gggttctcat
     1561 cgggcattta cgctgaaacc ggatgtcatt cacctggctg catggctatg cagccaggta
     1621 aaatttatct gtcgcgaacg gggcggttta tcgggtggtt tgttgccggt tttacctgac
     1681 tgccaccccg ttcgcggcga acccgtccgg ggcggtgcgg gcaacgggtg ttatgtttaa
     1741 tcagcgtgaa cggcagatac aaaaaagccc cggagggctt ttaactactg acttattttt
     1801 ttgaagattt gctgggtttc aatttgtcag tatttcccgg cgttttttta ttgtcacgct
     1861 ggcgtttatc aggttcccct gttgaatcgg caattcgttt cggacccggc ttaagaccca
     1921 taatcgttat cctttcaata gttaagagga aagccctcaa gctagatata ttgaccgcgt
     1981 tacggattat caagaggggc taacaagatt attttttgtt cttttaggtt gattgtggta
     2041 ctgttgatat acatgtatag caaatggtgt tcgggaggtt tatatgcttg ctatccggtt
     2101 atctgatgag attgagtccc gtctggactc gctggcgaag caaaccggca gaacaaagac
     2161 gttttatgcg cgggaagcaa tactggcgca tctggaagac ctggaggatt attatctttc
     2221 agcagaaact gctgcacgcg ttcgccgtgg tgatgaagca gtgcattcgt ctgaagacgt
     2281 gaggaagtca cttggtctgg acgattaact attccgatcg ggcgctcaaa tcgttacgca
     2341 agatggacaa acagaacgca cgacggattg tggattttat gagtttacgc attgcagttg
     2401 ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga gctgggcgag ttctggcgct
     2461 atcgggtggg agattatcgc gttctgtgtg agatccgaga tgacgagctt gttatccttg
     2521 ccgccacgat tggacatcgc cgcgaagttt acgactgaag cccgctatgc gggctttttt
     2581 gatgccgaca acaggtatta tggtaaatac tcgactaact acgtgttcta ctgtttgtcg
     2641 gttttagtta accagcaggt tgtcttaacg ataatgtagc cgttattttt aactaataag
     2701 gtcatattgt taacgtcagg cataataaaa gacattatgg cctcagagtt atcatcagaa
     2761 gcagctctct gcattactac ttcgattccc ttttctttga atctataagt atccagttgc
     2821 ctattaaact tagaaagacc tacaaccata cttagtttgt tatctttaac agtaagagat
     2881 gcttcctgag taccattaca tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg
     2941 gggaatataa atgctaggaa cgcaattaac caatacatag ttgagaatct tggtattaca
     3001 catttcacta tcctctcttt catttttcgt tcaccatact ttgttatctg atttttaatt
     3061 tttctatttt ataaaccaag tctctaccaa aaaggcgaaa attatcctca aataataata
     3121 attgtttaag aacatccatg aaagcagctg cttcaccctc agttatggga gctactctat
     3181 ataacatata acctgagtta agggtttctt ttatactttc ttcaatgctc attataaata
     3241 ttagtggatt atcatttttc ttcccccatg ggtttctaat taatccttcg atgtactcag
     3301 gtcctttgcg tcctattgcc tgcgcaatat tttttatttc atctgcgtga gggctttgtt
     3361 gtacagcttt gtttatgcct gcttgagtga ttttcagaga ctctacaccg tgtatgttgt
     3421 atgagataaa atgtaataaa ttaagatgca atgcaattaa tccatttttg tttacatctg
     3481 agtatttgga taaaaaacgt aatgaagtgt taactctccc ttgaagccta acaattggga
     3541 gtgctgcctt gtatatttcc ttctccgtta aataccgttt aaaaaacata attctccgcc
     3601 tccgtatagc gtgcctattt tatttcgtcg taaaacttag ctctgaaata ttcctttaga
     3661 accatcatgt cattaataat tgaatctatc tcatccattt tttcttggta ttcttttaat
     3721 ggtgaaggga gcggtaactc aatatttgaa aagtgattgg cgagataggg atcaaggttt
     3781 ctcatccatt caggcttttc gccaatgcgt tcttcaactg aaagtgaacc tgataatctc
     3841 tggttaataa gatcacttag actcattttg gttttattcg aaagccgttg taaggattgc
     3901 tttaatccta tatctagcat gatatttgtt ctttctctca ttaccccccc ttgctattgc
     3961 gttttttgtt gcgcacaaag ctaccattgc gcattaagaa acgcaataaa aaaggaggga
     4021 gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg
     4081 tgggatggat ggagctccgg ggataacaga actgacgatc atttaaagga tcttcttgag
     4141 atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tagggaggcg
     4201 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga
     4261 ccgcagatac caaaactgtt ctttcagtgt agccgcagtt aggcacccac ttcaagacct
     4321 ctcaatatct ctctcgtata tatccagtta ccaatggctg ctgccagtgg cgtttttgcg
     4381 tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga
     4441 acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac
     4501 cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt
     4561 atccggtaaa cggcagggtc ggaacaggag agcgcacgag ggagccgccg gagagaaatc
     4621 accggcatct ttaagtcctg tcgggtttcg tcctcctctg acttgagcgt ccatttctgt
     4681 gatgctcgtc aggggaggcg gagcctatgg aaaaacggca acgccgcatc cttttctccg
     4741 gccctttctg atttgagcgc ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc
     4801 cgcagccgaa cggcaggagc gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc
     4861 cgtgcatggt attgcggacg ctcctgagcc acatatcaca cctgccacat gaggcacttt
     4921 catgaatcgt gctctcatgc caacatagta agccagtata cacagcgtaa acgctgtgac
     4981 tggttcaggg ctgcgccccg aaacccgcta acaccactga cgcgctggcg cttgtccaac
     5041 gccggagcga cccggaaaac ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc
     5101 agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg
     5161 caaatctgat gcgtcaggca caggaggaag attaccgccg ggagcagatg gcagagcttg
     5221 agcgttcagc ccggtcacat tttgcggata tcggaggaga ggatggcggt taagcgtacc
     5281 cgttttttag gtatccgggt gaccgatggg gagtaccagc agctgctgga gcgctgcaat
     5341 ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg
     5401 ttgcggctgc cgtccatcga tccggttttg ctgcgccagc ttgccgggat ggggaacaac
     5461 ctcaaccaga tcgcccggaa aattaacggc ggccagtggt caggcgctga ccgggttcag
     5521 gtggtggccg cgctgatggc cattgatgcc gaactcgagc ggctgcggca tgccgtgctg
     5581 gaaaaggggg cagacg
//
