LOCUS       NZ_CP107377            11931 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_31 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP107377
VERSION     NZ_CP107377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207060
            Assembly: GCF_028220985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:44:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,016
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,713
            CDSs (with protein)               :: 5,713
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 69 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 25 of 181
            Pseudo Genes (multiple problems)  :: 33 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_31"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..1965
                     /locus_tag="OH494_RS29900"
     CDS             1..1965
                     /locus_tag="OH494_RS29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1965..2696
                     /gene="mobC"
                     /locus_tag="OH494_RS29905"
     CDS             1965..2696
                     /gene="mobC"
                     /locus_tag="OH494_RS29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2703..3233
                     /locus_tag="OH494_RS29910"
     CDS             2703..3233
                     /locus_tag="OH494_RS29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3260..3439)
                     /locus_tag="OH494_RS29915"
     CDS             complement(3260..3439)
                     /locus_tag="OH494_RS29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3465..3893)
                     /locus_tag="OH494_RS29920"
     CDS             complement(3465..3893)
                     /locus_tag="OH494_RS29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4057..4371)
                     /locus_tag="OH494_RS29925"
     CDS             complement(4057..4371)
                     /locus_tag="OH494_RS29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4359..4637)
                     /locus_tag="OH494_RS29930"
     CDS             complement(4359..4637)
                     /locus_tag="OH494_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4812..5177)
                     /locus_tag="OH494_RS29935"
     CDS             complement(4812..5177)
                     /locus_tag="OH494_RS29935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH494_RS29940"
     CDS             complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH494_RS29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5819..6064)
                     /locus_tag="OH494_RS29945"
     CDS             complement(5819..6064)
                     /locus_tag="OH494_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6691..8157
                     /locus_tag="OH494_RS29950"
                     /pseudo=""
     CDS             6691..8157
                     /locus_tag="OH494_RS29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /name="reverse transcriptase domain-containing protein"
     gene            8262..9947
                     /locus_tag="OH494_RS29955"
     CDS             8262..9947
                     /locus_tag="OH494_RS29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9957..10214
                     /locus_tag="OH494_RS29960"
     CDS             9957..10214
                     /locus_tag="OH494_RS29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10299..10448
                     /locus_tag="OH494_RS29965"
     CDS             10299..10448
                     /locus_tag="OH494_RS29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             3262..11931
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(10552..11130)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1966..2693
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt
       61 tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt ttttctggtt
      121 atcggcctgt ttttcggtct ggctgaactc tgggccggcc gaatgcgttt tgtggggctg
      181 agccataagg cgcggcggga gtatgccgat cgctataagc gcgataataa ccggactgtg
      241 ccattttcgg tgctggcctc gctggattca cccgggcgga tggtcagggc acggcgacat
      301 gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg
      361 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg
      421 aacgcagatt attacctgct gcagccggct aacctgttcg ggatagcctg gtgtgccgga
      481 gggcttgtcg ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat
      541 tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc
      601 cagcggaccg gagaaatgac agacgtccgc cacctgcatt tcggggagcc ggtaccggtt
      661 aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt gtttctgggt
      721 aaggatgagc agggccagcc ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt
      781 cagatcctcg gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc
      841 tgcgtacgcg atttcgggga cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg
      901 ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa
      961 ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt
     1021 gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag
     1081 aaagcggcga agctgattgc ggagcagttc ccgcaggggg caaacattca gcaggtgctg
     1141 gcggcggcgt acgcgttgcc ggaggccctg aaaaaggacg tgaaggggct gatcaccaag
     1201 ctggagaacg tggccgacct gagcgtcctg cagacggaca gcggtatcga tgtggcgggg
     1261 ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga ggcggtgatc
     1321 cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc
     1381 cggaggtggc cgcacgcgag catcatgctc gatgagatta aatatctgct gtcgaagtac
     1441 gtgctgaacg cgctggggac gctgcgcagc cgggactgca acctgctgct cgcgcaccag
     1501 tcgctgggcg acttcggtca gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg
     1561 gtactggata acacgccgat ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag
     1621 tgggcggcgg gccagaccgg ggagatccgg gtggacgtgg agcgccgccg ggccagccgg
     1681 gaggcgggga acgtggagca catcagcggc gacagcttca tccagaagga ggcgcggccg
     1741 ctgtttgacg tgaacaccct gcaacacctg cctgacgggt tcgcggtgat gaccgggctg
     1801 ggcgtggcgc ggctggcgtt cagcagcccg ctgcgggttg agcgccggga aatttcgctg
     1861 aaaaccttcc cggtgctggc gaaaacggac ccgctggcgg agtacctgcc tccggccgac
     1921 ggcggcggac caaaagcgga tgatgatttt gcggggcttt actgatgaca ctggagcagt
     1981 acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc
     2041 gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg
     2101 cggcggtaca gccggtttac cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc
     2161 gggcgaaata cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg
     2221 tggcgttctc gttcgacgag gacgagccgc tgacggacgt tattccgttt cagccgtcaa
     2281 gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg
     2341 agggccgggg ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag
     2401 tgccggacgc gctggcggag ctggacggca gaacggtggc atttgaggta gagcgcaccg
     2461 tcaagagccg caggcggtat caggaagtgg tgtccgggta tctgttcaac cgccgggcga
     2521 acggcatcga tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc
     2581 gggcgattct ggcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg
     2641 tggagctgga ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga
     2701 ttatggcgct gtacatggta tttattgagg acgtggaaga caaagacggc gagcgctgca
     2761 ttatgccaac cagcgagcat ctggaagaca tggtggtgct cgcctggcag accggggcgt
     2821 atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt
     2881 atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc
     2941 aatgggaacg ggacaaaccc acaggaatga agctgtgtga ttacctctat cgaaaggtgg
     3001 agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc
     3061 agattaacgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga
     3121 agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtgcctg
     3181 aactgccgga ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa taacgccagc
     3241 accggcatgg ccggtgctgt tattcttccc cgagcttatc ccgcatcacc tgccagagtg
     3301 tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg tccagctcgt
     3361 tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata ttgagggcag
     3421 ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct aaaccagccg
     3481 gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac ttcggcctgg
     3541 agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt tttatcttcc
     3601 agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc cagaagctgt
     3661 tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat ttgtttctca
     3721 gggatattca catttccgta tacacggatc agttctgaca catccacgac agggttgttt
     3781 ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag cgttcttctg
     3841 gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc cactgtatac
     3901 ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta tcgctgaatt
     3961 ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc aaaccggcga
     4021 cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg cccccggata
     4081 cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt cccggtcgtg
     4141 gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct tatattccag
     4201 cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct ggatagcccg
     4261 gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga gaaagtcctg
     4321 aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca tggcggagca
     4381 tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc cgcagtaagg
     4441 tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag gtactggctg
     4501 cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc gtgcgctgag
     4561 cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc gagcctgatg
     4621 gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat gactacttta
     4681 atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt cagcttcgct
     4741 tccactaatc agggctgcta caggctcact catcatccag gactattgca gcctgcgggt
     4801 taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt gactggtcgc
     4861 ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc
     4921 gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagg
     4981 tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc aaacgtggcg
     5041 gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg ctgcagtgtg
     5101 gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc cagcactgtc
     5161 agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt tgccaggtat
     5221 tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct gctttacaga
     5281 gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt tgtatggata
     5341 tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc atttcaacag
     5401 cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga gtataaattt
     5461 tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg cacaatgcca
     5521 gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa atgttacatg
     5581 tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat cgtatcgttt
     5641 ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt tacaatttac
     5701 cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg
     5761 gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta aatacccctt
     5821 accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac
     5881 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     5941 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     6001 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     6061 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     6121 atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt
     6181 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt
     6241 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     6301 aacgggagga gtaaggtaat aattatactg tgtatatgta cagtgtgcgc cgggagaccg
     6361 gttagagatc aaggggtgaa agtccccgac cattgaagga ccagcaatcc acaaggtccc
     6421 cgagtcatgc gttgcatacc gcgaggtatg gggcgaagcg ttgacggtgt tgacggagcc
     6481 agccattgag cctgaaatgt atattaaatt accgggtgcc gacgttgtac tgttaacgga
     6541 agagagcaac ataagtgcga cactgcgagc accgcatgga cccggcgggg tctgggaccc
     6601 tggcatgtca atacgatctc tacgcgggaa ccggagatcg ccctctgacc atctgccagt
     6661 gtcggagatg gcccacgcga gtcatagccg gtgatgtacg gagaggagaa gtcggactcg
     6721 ctcatagtag cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg
     6781 cagggccaag gggaacgcgg aacaatacat gcgccggaca cagagccgga aagcatgtca
     6841 cagaggctgt cacgcgtgcg ggagaagctg cgaagcagcg gaagaaagaa cggtttacag
     6901 cattgttcca cctgctgacg gtcagaagca ctggaagccg cattctcctg agcagggaaa
     6961 gcggccgccg gagtggatgg catcaggtgg atggacctac gccaggctca tgaagaacga
     7021 cgcgccaaca gatctgccgg aaattcctgt caggggcagc tggggcgcag cccggcaggc
     7081 gtcactacat cccaaaagcg gatggaaaac aacgcccgct cggcatcgcc tcgctggagg
     7141 acaagatcgt cggtatgcgc tggtgaaaat cctgaacacg cagtctatag aaaacgctat
     7201 gcggggttct catacgggtt cagacccggg cgaaaaccag cacgatactg gacgcactgg
     7261 cctggggctg ggtacgcact aacgtaaact gggtactgga tgccgacatc agtcagttct
     7321 tcgacgggtg agccacgaat ggctgatcag gttctaagga tcggcgaccg gagggtaatc
     7381 gagctcatac gtagaagtgg ctcacagccg ggacgtcgga gaggggtcaa tggcgagcaa
     7441 cggagaaggc accccacagg ggtgcggtca tctcaccgct gctggcaaac atatacctcc
     7501 actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccacg ggcaatgtgg
     7561 taatggtcag atacgccgat gacatcgtca tcaggtcgac aaacttgacc gatgcccggc
     7621 gcttcccgta taaccgtagc gcagacttca gggattcgga ctcacggttc acccggagaa
     7681 aacccgtctg atggagttcg gccgcttcgc tgccgaaaac cgtgccatcg ggggaaaagg
     7741 caaataagcg ttcaacttcc tcgggttcac gcatcagcag gaaagatcgc aacggcaggt
     7801 tcatgctgtt acagaaagac ccgccgggat cggatgagca ggctctgaaa gccatcaaag
     7861 acggtctgcg aaggcgctgg cattactcaa atccccgaac aggaaaatgg ctcaggagag
     7921 tggttcaggg atacctgaac tatcactctc ggcgaacgct tccccaccat gtgcagaagt
     7981 tcaggacaca tggcgcaaca aacctctggc gccgggcgct caggcacggg agccaggatg
     8041 acgacctgga cagcaaacaa actggcagcc gcatggctac caagggttcg ggttcttcat
     8101 ccatggcctg tggagcggtt caccgccaga cacccgaggc aggagcccgg tgcgtaaatc
     8161 gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc gcgacatgtt
     8221 ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc
     8281 gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg
     8341 gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg
     8401 gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg
     8461 gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct
     8521 ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc ctggctactc
     8581 cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg
     8641 atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt gcgatctacg
     8701 gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt
     8761 cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg gaccaggcga
     8821 cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg
     8881 ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg
     8941 gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg
     9001 ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg
     9061 ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg
     9121 gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc
     9181 gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc
     9241 tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa aatattgcga
     9301 ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg gctgctgttc
     9361 aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt gtgacttata
     9421 acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata
     9481 ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat
     9541 ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa caaaaggaga
     9601 aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga
     9661 ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg
     9721 gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc
     9781 gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
     9841 aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc
     9901 aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg
     9961 ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt
    10021 attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg
    10081 ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat
    10141 tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg
    10201 acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca
    10261 gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt
    10321 ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc
    10381 gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
    10441 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
    10501 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
    10561 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
    10621 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
    10681 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt tcccgggttg
    10741 gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc
    10801 ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat
    10861 gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt aaacggcagg
    10921 gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga tctttaagtc
    10981 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
    11041 accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
    11101 caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
    11161 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
    11221 ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc ggacgcgctg
    11281 atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat tatcaggata
    11341 ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg ttgctttttt
    11401 ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca ggggaaaaga
    11461 ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt tctggctctg
    11521 tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct cacgcctgac
    11581 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
    11641 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
    11701 accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg
    11761 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc
    11821 aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc
    11881 gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg c
//
