LOCUS       NZ_CP107371             4746 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_32 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP107371
VERSION     NZ_CP107371.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207061
            Assembly: GCF_028220965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:44:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,494
            Genes (coding)                    :: 5,372
            CDSs (with protein)               :: 5,372
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 45 of 122
            Pseudo Genes (incomplete)         :: 78 of 122
            Pseudo Genes (internal stop)      :: 14 of 122
            Pseudo Genes (multiple problems)  :: 15 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107371.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_32"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..981
                     /locus_tag="OH524_RS27990"
     CDS             1..981
                     /locus_tag="OH524_RS27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="WP_013213985.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQN
                     RESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGLGR
                     CLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFVAID
                     RATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKKKEPS
                     GTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELESTLRNY
                     VKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
                     /name="IS481-like element ISKpn27 family transposase"
     gene            1257..2138
                     /locus_tag="OH524_RS27995"
     CDS             1257..2138
                     /locus_tag="OH524_RS27995"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(2388..>3269)
                     /locus_tag="OH524_RS28000"
                     /pseudo=""
     CDS             complement(2388..>3269)
                     /locus_tag="OH524_RS28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            3407..4111
                     /locus_tag="OH524_RS28005"
     CDS             3407..4111
                     /locus_tag="OH524_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <4163..4624
                     /locus_tag="OH524_RS28010"
                     /pseudo=""
     CDS             <4163..4624
                     /locus_tag="OH524_RS28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     ARG             1258..2135
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 atgacccagg cactgcacag ccaagcccgt actacccacc tgatccgtga ggaaatcagg
       61 aactcgacgc tcccgcaggc cgaactggcc aggatgtaca acgtcacccg ccaaaccatc
      121 cgaaagtggc aaaaccgcga gtctcctgaa gacaagtcgc atgcgccgaa caagatgtac
      181 acgacgctca cgcccgagca ggagctcatc gtggtggagc tgcgcaagac gttgctgctg
      241 cccacggacg acctgctggc ggtcacgcgc gagttcatca atccagccgt ctcgcgtgcc
      301 ggcctgggac gttgcctgcg ccgccacggt gtctcggatc tacgtaacct ggtcgagcag
      361 gaaggcactg cgcccgccac gaaaaagacc ttcaaggact acgagccggg ctttgtgcac
      421 atcgacatca agtacctgcc gcagatgccc gacgagacgg caaggcgcta tctcttcgtt
      481 gccatcgacc gtgctacgcg ctgggtcttc atcgagctct atgccgacca gaccgatggc
      541 agcagtggcg acttcctcaa caaagtccag caagcctgtc ccgtcaagat cgtcaagctt
      601 ctgaccgaca acggcagcca gttcaccgac cgcttcacgg ctggcggcaa gaagaaggaa
      661 cccagcggca cacacgtgtt cgaccgcctg tgcaagcagc tcggcatcga gcaccggctc
      721 atcccgcctc gtcatccgca gaccaacggc atggtggagc gcttcaacgg tcgtatcagc
      781 gacatcgtca accagacccg ttttggttca gctgccgaac tggaatcgac gctgcgcaat
      841 tacgtcaaga tctacaacca cagcattccg caacgcgcgc tccaacacaa aacacccgtt
      901 caggcgctca aggaatggca tgaaaaacgc cctgaattgt tcaggaaacg cgtgtataac
      961 cagccgggtc ttgacatata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaatg
     1141 acttcttaac gtgagttttc gttccactga gcgtcagacc cctaatccca gctgtagcgg
     1201 cctgattaca tccggccgct acacctagct ccaccttcaa acaaggaata tcgttgatgt
     1261 cactgtatcg ccgtctagtt ctgctgtctt gtctctcatg gccgctggct ggcttttctg
     1321 ccaccgcgct gaccaacctc gtcgcggaac cattcgctaa actcgaacag gactttggcg
     1381 gctccatcgg tgtgtacgcg atggataccg gctcaggcgc aactgtaagt taccgcgctg
     1441 aggagcgctt cccactgtgc agctcattca agggctttct tgctgccgct gtgctggctc
     1501 gcagccagca gcaggccggc ttgctggaca cacccatccg ttacggcaaa aatgcgctgg
     1561 ttccgtggtc acccatctcg gaaaaatatc tgacaacagg catgacggtg gcggagctgt
     1621 ccgcggccgc cgtgcaatac agtgataacg ccgccgccaa tttgttgctg aaggagttgg
     1681 gcggcccggc cgggctgacg gccttcatgc gctctatcgg cgataccacg ttccgtctgg
     1741 accgctggga gctggagctg aactccgcca tcccaggcga tgcgcgcgat acctcatcgc
     1801 cgcgcgccgt gacggaaagc ttacaaaaac tgacactggg ctctgcactg gctgcgccgc
     1861 agcggcagca gtttgttgat tggctaaagg gaaacacgac cggcaaccac cgcatccgcg
     1921 cggcggtgcc ggcagactgg gcagtcggag acaaaaccgg aacctgcgga gtgtatggca
     1981 cggcaaatga ctatgccgtc gtctggccca ctgggcgcgc acctattgtg ttggccgtct
     2041 acacccgggc gcctaacaag gatgacaagc acagcgaggc cgtcatcgcc gctgcggcta
     2101 gactcgcgct cgagggattg ggcgtcaacg ggcagtaagg ctctgaaaat catctattgg
     2161 cccaccaccg ccgcccttgc gggcggcatg gattaccaac cactgtcaca tttaggctag
     2221 gagtctgcgc ggcagagccg tgtgaccggt tttctgtaga gcactgacga tggcggcggc
     2281 gctctctgca attggcaagg cgtcggcgcc aaggatacca atcttgcggc gcgcggcgtg
     2341 ttatgacgac tggggtgcat ttgagccgcc ccatttaacc ttcgccctca cagatacgcc
     2401 attcgcctca gatttagcgc catgcagacg agcttccact cggcttgcac cttgtccagg
     2461 cccctcatgc tgaactgacg caatcccatc accgccttga tccaaccatt cggagcctcc
     2521 acgatcgact tgcgccggcg gtaagctgca tcgccttgct ccgttttcaa tttcgccgca
     2581 atcgccgccg tatgcggatg ggtcttggca ttgaccttgg catcttcacg tccctcgcgg
     2641 ccgagggcaa cgatgacatc gccgtggtga tcggcgacct ttgccagaac agcctcacta
     2701 cggaatcccg catccgccag cgtctgggcc ggcatttctc cggtgttggc ctgaactgct
     2761 gccagcatgc ccagcagcgc ctgactgtcc gcggcgcagt tggtcaactc cgccgccacg
     2821 atgatctggt gctcggcatc gaccgctgtg tacccgttgt agctctgctc ggagccacca
     2881 ccggcgtgtt tcatgatccg gctgtccgga tcggtgaagc tttcctgatc acggtcatcc
     2941 ggcacaccaa actcgcgttt gtacgagcca ccgcccttgt ccgagccatc cggatggcga
     3001 ggccggcggc catcgtcttc gctgcgcccc cgggcctggt ccgcttcacg ctggcgcgct
     3061 tccaggcgcg cctttgccgc ctggatcgcc tccaggcgct tctcgcggcg agaaatctcg
     3121 gcaggaatgt ccagctccgg ctcgttacgc tcctggtcgt cggtagcctt ggcgcgatca
     3181 agcagcgcct tgatctcgca atgcaattcg tcctcggccg gcttcatgcg cttatagctc
     3241 atcgccttgt ggtcgccatg tttggcggct tcgtattcag gttctttaga gagatccttt
     3301 agatcgccgt tcctcactac tgccgggaag tctccccagg gagggcactg ttgcaaatag
     3361 tcggtggtga taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa
     3421 aggccggcat tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg
     3481 catcagttac cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc
     3541 cacgatttac cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta
     3601 ctggcgtaac ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa
     3661 tggccgctgg gcgtatctgt accgggccgt cgacagccgg ggccgcactg tcgattttta
     3721 tctctcctcc cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa
     3781 cgtgaagaag tggcagatcc cgcgattcat caacacggat aaagcgcccg cctatggtcg
     3841 cgcgcttgct ctgctcaaac gcgaaggccg gtgcccgtct gacgttgaac accgacagat
     3901 taagtaccgg aacaacgtga ttgaatgcga tcatggcaaa ctgaaacgga taatcggcgc
     3961 cacgctggga tttaaatcca tgaagacggc ttacgccacc atcaaaggta ttgaggtgat
     4021 gcgtgcacta cgcaaaggcc aggcctcagc attttattat ggtgatcccc tgggcgaaat
     4081 gcgcctggta agcagagttt ttgaaatgta aggcctttga ataagacaaa aggctgcctc
     4141 atcgctaact ttgcaacagt gcctctttac cgacaaggca tccggcagtt caacagatcg
     4201 ggaagggctg gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggtgaagaa
     4261 gctcgaccgt cttggccgcg acaccgccga catgatccaa ctgataaaag agtttgatgc
     4321 tcagggtgtc gcggttcggt ttattgacga cgggatcagt accgacggtg atatggggca
     4381 aatggtggtc accatcctgt cggctgtggc acaagctgaa cgccggagga tcctagagcg
     4441 cacgaatgag ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac
     4501 cgtggacagg aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc
     4561 tcatcagctc agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc
     4621 gtgatacgcc tatttttata ggtgtctgga ctcgtgggat catgtaccca tgcgtagctg
     4681 gccgctcttc aaggccagac acgttttgcg tacactgttc caatccgact cttcactggc
     4741 aactcg
//
