LOCUS       NZ_CP107365            10060 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_33 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP107365
VERSION     NZ_CP107365.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207062
            Assembly: GCF_028220865.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:42:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,558
            CDSs (total)                      :: 5,436
            Genes (coding)                    :: 5,306
            CDSs (with protein)               :: 5,306
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 48 of 130
            Pseudo Genes (incomplete)         :: 85 of 130
            Pseudo Genes (internal stop)      :: 14 of 130
            Pseudo Genes (multiple problems)  :: 17 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107365.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_33"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..258
                     /locus_tag="OH511_RS27610"
     CDS             1..258
                     /locus_tag="OH511_RS27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            343..492
                     /locus_tag="OH511_RS27615"
     CDS             343..492
                     /locus_tag="OH511_RS27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1976..3940
                     /locus_tag="OH511_RS27620"
     CDS             1976..3940
                     /locus_tag="OH511_RS27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3940..4671
                     /gene="mobC"
                     /locus_tag="OH511_RS27625"
     CDS             3940..4671
                     /gene="mobC"
                     /locus_tag="OH511_RS27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4678..5208
                     /locus_tag="OH511_RS27630"
     CDS             4678..5208
                     /locus_tag="OH511_RS27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5235..5414)
                     /locus_tag="OH511_RS27635"
     CDS             complement(5235..5414)
                     /locus_tag="OH511_RS27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5440..5868)
                     /locus_tag="OH511_RS27640"
     CDS             complement(5440..5868)
                     /locus_tag="OH511_RS27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6032..6346)
                     /locus_tag="OH511_RS27645"
     CDS             complement(6032..6346)
                     /locus_tag="OH511_RS27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6334..6612)
                     /locus_tag="OH511_RS27650"
     CDS             complement(6334..6612)
                     /locus_tag="OH511_RS27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6787..7152)
                     /locus_tag="OH511_RS27655"
     CDS             complement(6787..7152)
                     /locus_tag="OH511_RS27655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7149..7520)
                     /gene="tolA"
                     /locus_tag="OH511_RS27660"
     CDS             complement(7149..7520)
                     /gene="tolA"
                     /locus_tag="OH511_RS27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7794..8039)
                     /locus_tag="OH511_RS27665"
     CDS             complement(7794..8039)
                     /locus_tag="OH511_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8366..10051
                     /locus_tag="OH511_RS27670"
     CDS             8366..10051
                     /locus_tag="OH511_RS27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3366..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(596..1174)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3941..4668
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa
       61 gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta
      121 aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag
      181 ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat
      241 cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg
      301 ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact
      361 atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat
      421 atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc
      481 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      541 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
      601 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
      661 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
      721 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg
      781 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
      841 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
      901 ctatgagaaa gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg
      961 cagggtcgga acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta
     1021 agtcctgtcg ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag
     1081 atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa
     1141 acgccaccgg cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga
     1201 atctgtgggt aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta
     1261 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc
     1321 gctgatgtgg gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag
     1381 gataggtgaa ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
     1441 ttttctcctt gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa
     1501 aagaccttgc ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc
     1561 tctgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
     1621 tgaccaccca caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct
     1681 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
     1741 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
     1801 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga
     1861 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
     1921 cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt
     1981 taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc
     2041 gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg
     2101 cctgtttttc ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca
     2161 taaggcgcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt
     2221 ttcggtgctg gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg
     2281 gaactacgcg tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt
     2341 cgccgtgttt atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc
     2401 agattattac ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct
     2461 tgtcgccggc gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc
     2521 ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg
     2581 gaccggagaa atgacagacg tccgccacct gcatttcggg gagccggtac cggttaacgc
     2641 gctggccgat ttttccacgg agcaggcccg gaaacagcag gcggtgtttc tgggtaagga
     2701 tgagcagggc cagccggtgc tggtccctcg tgatacctgg cgcaagacca atattcagat
     2761 cctcggcctg cccgggagcg gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt
     2821 acgcgatttc ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca
     2881 cgtcttccgg gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc
     2941 gcagctgaat ctgttccgcg atctcgacca gtacgccctg aagaatctgc tggttgccgg
     3001 gtttaacctg agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc
     3061 ggcgaagctg attgcggagc agttcccgca gggggcaaac attcagcagg tgctggcggc
     3121 ggcgtacgcg ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga
     3181 gaacgtggcc gacctgagcg tcctgcagac ggacagcggt atcgatgtgg cggggataat
     3241 caacggcggc ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt
     3301 gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg agttccggag
     3361 gtggccgcac gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct
     3421 gaacgcgctg gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct
     3481 gggcgacttc ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact
     3541 ggataacacg ccgatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc
     3601 ggcgggccag accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc
     3661 ggggaacgtg gagcacatca gcggcgacag cttcatccag aaggaggcgc ggccgctgtt
     3721 tgacgtgaac accctgcaac acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt
     3781 ggcgcggctg gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac
     3841 cttcccggtg ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg ccgacggcgg
     3901 cggaccaaaa gcggatgatg attttgcggg gctttactga tgacactgga gcagtacaac
     3961 gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg
     4021 ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg
     4081 gtacagccgg tttaccgctt cctggacagt ctggtggcga aagggctgct ggtccgggcg
     4141 aaatacccgg ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg
     4201 ttctcgttcg acgaggacga gccgctgacg gacgttattc cgtttcagcc gtcaagggtg
     4261 tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgcctctc catggagggc
     4321 cggggggcca ccggctggcg gtacctgcac cgtatggccc tgaaagggat gaaagtgccg
     4381 gacgcgctgg cggagctgga cggcagaacg gtggcatttg aggtagagcg caccgtcaag
     4441 agccgcaggc ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc
     4501 atcgatgaaa tctggtacat ctgccctgac cggccgacgc ttgtccgggt acagcgggcg
     4561 attctggcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggtggag
     4621 ctggaccggg agaggctgtt cgcgtgcttt aaatttatga caacggagtg agggattatg
     4681 gcgctgtaca tggtatttat tgaggacgtg gaagacaaag acggcgagcg ctgcattatg
     4741 ccaaccagcg agcatctgga agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt
     4801 ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac
     4861 gagcggctca acagcgacga aaaagccacg tactttttca ccggtgaaaa tccgcaatgg
     4921 gaacgggaca aacccacagg aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg
     4981 ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt
     5041 aacgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg
     5101 aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg
     5161 ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg
     5221 catggccggt gctgttattc ttccccgagc ttatcccgca tcacctgcca gagtgtttcc
     5281 gcatcctcgt gaagccgttc gcacatgtcg gccggggtat ccatgtccag ctcgttcagc
     5341 ttttccagca acagcagcga ctgatcctta atgaatttag ccatattgag ggcagttttt
     5401 tcctgcttcg tcatgttctc cattcccttc ggtaagttct cagctaaacc agccggacca
     5461 gaagcgtttt ttcttctgtg tcagttctgt tgtcaatctg tccacttcgg cctggagttt
     5521 ccggcgctca tcatcgtgct ggcggcgttc ttcttcccgg gatgttttat cttccagcat
     5581 cagggtgaca gcctgccgga gatcggccag ttccttttgc attgccagaa gctgttcctg
     5641 tgggaaattt ttttgtgtat tttcacggtg tgaaatgccg ggaatttgtt tctcagggat
     5701 attcacattt ccgtatacac ggatcagttc tgacacatcc acgacagggt tgtttttccc
     5761 gtcacgtgac acggtaacct ttccctgttt tacatgattg tatagcgttc ttctggtaat
     5821 gcccgcggct tgtgctgcct gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg
     5881 attttatcgg gtgtgaaagg tgtgtaattt ctcacggtaa atgtatcgct gaattttacc
     5941 cgtttttccg ttcaggatgt ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc
     6001 acgcaatgaa ttgcgccccc gtcatcggtt attagaggct gtctgccccc ggatacccgg
     6061 attctttctg atgcctgacg gtgacgatca caatctggtc catttcccgg tcgtggcggt
     6121 agagcatcac atagccactg tcgccaaacc cgatcaccag ttccttatat tccagcggca
     6181 gaaacggcac cgggcggcca atgtctggca atgttttcag ttgctggata gcccggacga
     6241 tcacctcacc tccttttctg gctgccagcc gatttttggt tttgagaaag tcctgaaggc
     6301 gttgtaaatc gcgctgtgcc agcgcggaaa tcgttacctg tggcatggcg gagcatcctg
     6361 ctcgttctca gttccccagg tacttatcca ggcttccgct tcttccgcag taaggtgcag
     6421 acccgtttcc tgatagtgct gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt
     6481 ctcttcccgg tcgatgtatt cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg
     6541 ccggtcatcg gccagcgttt tcagccggtc cttaagttct tcatcgagcc tgatggatgt
     6601 tgctgatgac ataaaagccc ctttgtagtc atatgtatta cacatgacta ctttaatccg
     6661 tggcggcgct atttgccaga aatacggctt ttgctgccca tttttcagct tcgcttccac
     6721 taatcagggc tgctacaggc tcactcatca tccaggacta ttgcagcctg cgggttaaag
     6781 gacctgttac tggcggcaac catcatcatt gagcttttgg gtggtgactg gtcgcggatc
     6841 ctgtaagccg tcatttttca gactgaattc aaaatttata acctgtcgca ttgccgggtg
     6901 ccctgcggga tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca
     6961 gccggtaagc gacacgttgt ccactcgtcc atcgtccaga atatcaaacg tggcggtcac
     7021 ctgaccttcg atgcgttttg ctgcggcctg taccgggtag gttggctgca gtgtggttac
     7081 gggttttagt ggtatcgcat gtaccggagt gaggaacatg gccgccagca ctgtcagagt
     7141 aaaagctttc atggtctgaa atccagaggg gcgtttttga atatttgcca ggtattttca
     7201 tccggtgccg gcggaatttt ggcgtgggta acggctgata ttgctgcttt acagagtttc
     7261 ggatctcctc cttcagcaat ggcgctgttc agagtgccat cacgttgtat ggatatccgc
     7321 acggtgcaca tttttcccgc ataggcctct ggttcaaaca tctgcatttc aacagcggtc
     7381 ttgatggccg cagcgtagct actgatttta gatgacttat ccagagtata aattttcggc
     7441 ggctgggtgg tggaatgctt acaggccgtc aggagtaagg ggctgcacaa tgccagcagg
     7501 ataaagggta ttaaacgcat cgggttgatg acctgtcagt aaaaaatgtt acatgtatat
     7561 ttgtcactct ttacggccac gttcatttac ataaacacgg cttatcgtat cgtttttaca
     7621 ccaaacggcc cgccatgcag ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc
     7681 gcgtttccgg cggttttccg ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac
     7741 gccctcagac cgtctgagcg gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg
     7801 cggcgctgtg gctgccaggt gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt
     7861 tcctgcgccc gcctggcttc acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc
     7921 tgttgtttta ccgcctttgg cagcgttttg ttccctttca ggcccatggc cagtgcctga
     7981 gcgacaaagg cgtccgccag gcgcttttct tcagcgggag tcagtttcag gtgcgtcata
     8041 gtggcctccg ggcgtctcag gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc
     8101 agttagcttt cagatgtgtc tataccatct gaaatgtaag tgtgatgaat gtcttaaata
     8161 ataaaatttg ttgccatgag aaattttcgg gaaaagacgc gatctgctta caggtggctc
     8221 cgggcgcttt tttgtgcctg aagatgagaa ctaatctcat atattgacac ctgaaaacgg
     8281 gaggagtaag gtaataatta tactgtgtat atgtacagta tgttggcttt aatttattgt
     8341 tttaaatgtc aaagaggaaa acggtatgag tggtggagac ggtcgaggtc cgggtaattc
     8401 aggtctggga cataatggtg gtcaggccag tgggaatgtg aacggtacgt ctggtaaagg
     8461 tggcccttca tcaggtggtg gtacggatcc aaacagcggg ccgggctggg gtacgacgca
     8521 tacgccgaat ggagatattc ataactataa tccgggggag tttggtaacg gcgggagtaa
     8581 acccggtggt aatggtggta acagcggcaa tcatagcggt agctctggtg gcggacagtc
     8641 ttcggccact gcgatggcct tcggtctgcc tgccctggct actccggggg ctgaaggact
     8701 ggctttatca gtttccggcg atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct
     8761 gaaggggccg tttaagtttg gtctgtgggg gattgcgatc tacggtgtgc tgccttctga
     8821 gattgcaaaa gatgatccga aaatgatgtc aaaaattatg acgtcattac cggccgatgc
     8881 ggtgacggag actccggcaa gtactttacc actggaccag gcgacggttc gtgtcagaca
     8941 acgggttgtg gatgtggtga aggatgagcg gcagcatatt gcggttgtcg caggtcggcc
     9001 aatgagtgtc cctgtggtgg atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc
     9061 gattccgggt ctcccgtctc tgcaggtgag cgtaccgaaa ggtgttccga cagcgaaagc
     9121 cccgccaaaa ggcattgttg ctgaaaaagg tgattcacgt ccggctggtt ttacagccgg
     9181 tggtaactcc cgtgaggccg ttattcgttt cccgaaagag accggacaga agccggttta
     9241 tgtgtcggtg acagatgttc ttaccccggc acaggtaaaa cagcgtcagg aggaagaaaa
     9301 gcgtcgccag caggcatggg acgccgctca tccggaagag gggctgaaaa gagactatga
     9361 taaagcgaaa gctgagctgg atgccgaaga taaaaatatt gcgaccttaa acagccgcat
     9421 tgcatcgaca gagaaggcgc tccccggtgc aagggctgct gttcaggaag ccgataaaaa
     9481 ggtaaaagag gcagaggcaa ataaagatga ttttgtgact tataacccgc ctcatgaata
     9541 tggctccggg tggcaggatc aggttcgcta tcttgataag gatattcaga atcagaatga
     9601 gaaattaaaa gcggctcagg catctttaaa cgcaatgaat gaatccttat ccagagataa
     9661 ggctgcactt accggggcga tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc
     9721 agaaaataag ttaaatgagg aaaagaacaa gcctcgcaag gggactaaag actacggcca
     9781 tgattatcat ccagtaccta aaacagaaga cataaaggga cttggagagt taaagcgagg
     9841 tgatcctaaa acacctaaac aaggtggtgg tggtaagcgg gcccgatggt atggcgataa
     9901 aaaacgtaaa atttatgaat gggattccca gcacggtgag cttgaagggt atcgtgccag
     9961 tgatggcgaa cacctaggcg catttgatcc caaaacgggt aagcaggtta aagggccaga
    10021 tccaaaacgg aacattaaaa aatatctttg agaggtaaat
//
