LOCUS       NZ_CP107339            11970 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_38 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP107339
VERSION     NZ_CP107339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207067
            Assembly: GCF_028219995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:54:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,016
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,715
            CDSs (with protein)               :: 5,715
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 67 of 179
            Pseudo Genes (incomplete)         :: 120 of 179
            Pseudo Genes (internal stop)      :: 24 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_38"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..1965
                     /locus_tag="OH514_RS29900"
     CDS             1..1965
                     /locus_tag="OH514_RS29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1965..2696
                     /gene="mobC"
                     /locus_tag="OH514_RS29905"
     CDS             1965..2696
                     /gene="mobC"
                     /locus_tag="OH514_RS29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2703..3233
                     /locus_tag="OH514_RS29910"
     CDS             2703..3233
                     /locus_tag="OH514_RS29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3260..3439)
                     /locus_tag="OH514_RS29915"
     CDS             complement(3260..3439)
                     /locus_tag="OH514_RS29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3465..3893)
                     /locus_tag="OH514_RS29920"
     CDS             complement(3465..3893)
                     /locus_tag="OH514_RS29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4057..4371)
                     /locus_tag="OH514_RS29925"
     CDS             complement(4057..4371)
                     /locus_tag="OH514_RS29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4359..4637)
                     /locus_tag="OH514_RS29930"
     CDS             complement(4359..4637)
                     /locus_tag="OH514_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4812..5177)
                     /locus_tag="OH514_RS29935"
     CDS             complement(4812..5177)
                     /locus_tag="OH514_RS29935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH514_RS29940"
     CDS             complement(5174..5545)
                     /gene="tolA"
                     /locus_tag="OH514_RS29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5819..6064)
                     /locus_tag="OH514_RS29945"
     CDS             complement(5819..6064)
                     /locus_tag="OH514_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6709..8196
                     /gene="ltrA"
                     /locus_tag="OH514_RS29950"
     CDS             6709..8196
                     /gene="ltrA"
                     /locus_tag="OH514_RS29950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            8301..9986
                     /locus_tag="OH514_RS29955"
     CDS             8301..9986
                     /locus_tag="OH514_RS29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9996..10253
                     /locus_tag="OH514_RS29960"
     CDS             9996..10253
                     /locus_tag="OH514_RS29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10338..10487
                     /locus_tag="OH514_RS29965"
     CDS             10338..10487
                     /locus_tag="OH514_RS29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             3301..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(10591..11169)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1966..2693
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgtttaata cggattcgct tgcctggcag tggcatatcc cggagctggc ccggcagatt
       61 tacccgtggg tcagctggct gtaccgggag tattacagtc tggccggttt ttttctggtt
      121 atcggcctgt ttttcggtct ggctgaactc tgggccggcc gaatgcgttt tgtggggctg
      181 agccataagg cgcggcggga gtatgccgat cgctataagc gcgataataa ccggactgtg
      241 ccattttcgg tgctggcctc gctggattca cccgggcgga tggtcagggc acggcgacat
      301 gtctggaact acgcgtactg gctgaccggc gctgccggtc tggcccttct gggggggctg
      361 gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgcccgga cctctatgcg
      421 aacgcagatt attacctgct gcagccggct aacctgttcg ggatagcctg gtgtgccgga
      481 gggcttgtcg ccggcgtcct tctggcgcgg ctgctggcgt tcctgctgct tgatggtcat
      541 tttgcggccg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc
      601 cagcggaccg gagaaatgac agacgtccgc cacctgcatt tcggggagcc ggtaccggtt
      661 aacgcgctgg ccgatttttc cacggagcag gcccggaaac agcaggcggt gtttctgggt
      721 aaggatgagc agggccagcc ggtgctggtc cctcgtgata cctggcgcaa gaccaatatt
      781 cagatcctcg gcctgcccgg gagcggcaag agcgcgatgg ccaccaacgc gctcattcgc
      841 tgcgtacgcg atttcgggga cgctgtggtg tacttcgacc ccaaagggga cgcgtgggcg
      901 ccgcacgtct tccgggcgca ctgcccggac ttcaccctgc tggatctgcg gccggggaaa
      961 ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg ccctgaagaa tctgctggtt
     1021 gccgggttta acctgagtga gaccggcgac gtggcggacc actaccgcat cagcgagcag
     1081 aaagcggcga agctgattgc ggagcagttc ccgcaggggg caaacattca gcaggtgctg
     1141 gcggcggcgt acgcgttgcc ggaggccctg aaaaaggacg tgaaggggct gatcaccaag
     1201 ctggagaacg tggccgacct gagcgtcctg cagacggaca gcggtatcga tgtggcgggg
     1261 ataatcaacg gcggcggctg cctgtacgtc atcggctcga tggacgatga ggcggtgatc
     1321 cgggtgcaga agatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg ggatgagttc
     1381 cggaggtggc cgcacgcgag catcatgctc gatgagatta aatatctgct gtcgaagtac
     1441 gtgctgaacg cgctggggac gctgcgcagc cgggactgca acctgctgct cgcgcaccag
     1501 tcgctgggcg acttcggtca gtgcgggcag gatctgcccg ctgattttgt gaaaaccacg
     1561 gtactggata acacgccgat ccgctggttc tatcgcgcgg ccagccagga gtccgcgcag
     1621 tgggcggcgg gccagaccgg ggagatccgg gtggacgtgg agcgccgccg ggccagccgg
     1681 gaggcgggga acgtggagca catcagcggc gacagcttca tccagaagga ggcgcggccg
     1741 ctgtttgacg tgaacaccct gcaacacctg cctgacgggt tcgcggtgat gaccgggctg
     1801 ggcgtggcgc ggctggcgtt cagcagcccg ctgcgggttg agcgccggga aatttcgctg
     1861 aaaaccttcc cggtgctggc gaaaacggac ccgctggcgg agtacctgcc tccggccgac
     1921 ggcggcggac caaaagcgga tgatgatttt gcggggcttt actgatgaca ctggagcagt
     1981 acaacgtcag ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc
     2041 gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg
     2101 cggcggtaca gccggtttac cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc
     2161 gggcgaaata cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg
     2221 tggcgttctc gttcgacgag gacgagccgc tgacggacgt tattccgttt cagccgtcaa
     2281 gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg
     2341 agggccgggg ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag
     2401 tgccggacgc gctggcggag ctggacggca gaacggtggc atttgaggta gagcgcaccg
     2461 tcaagagccg caggcggtat caggaagtgg tgtccgggta tctgttcaac cgccgggcga
     2521 acggcatcga tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc
     2581 gggcgattct ggcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg
     2641 tggagctgga ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga
     2701 ttatggcgct gtacatggta tttattgagg acgtggaaga caaagacggc gagcgctgca
     2761 ttatgccaac cagcgagcat ctggaagaca tggtggtgct cgcctggcag accggggcgt
     2821 atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt
     2881 atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc
     2941 aatgggaacg ggacaaaccc acaggaatga agctgtgtga ttacctctat cgaaaggtgg
     3001 agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc
     3061 agattaacgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga
     3121 agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtgcctg
     3181 aactgccgga ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa taacgccagc
     3241 accggcatgg ccggtgctgt tattcttccc cgagcttatc ccgcatcacc tgccagagtg
     3301 tttccgcatc ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg tccagctcgt
     3361 tcagcttttc cagcaacagc agcgactgat ccttaatgaa tttagccata ttgagggcag
     3421 ttttttcctg cttcgtcatg ttctccattc ccttcggtaa gttctcagct aaaccagccg
     3481 gaccagaagc gttttttctt ctgtgtcagt tctgttgtca atctgtccac ttcggcctgg
     3541 agtttccggc gctcatcatc gtgctggcgg cgttcttctt cccgggatgt tttatcttcc
     3601 agcatcaggg tgacagcctg ccggagatcg gccagttcct tttgcattgc cagaagctgt
     3661 tcctgtggga aatttttttg tgtattttca cggtgtgaaa tgccgggaat ttgtttctca
     3721 gggatattca catttccgta tacacggatc agttctgaca catccacgac agggttgttt
     3781 ttcccgtcac gtgacacggt aacctttccc tgttttacat gattgtatag cgttcttctg
     3841 gtaatgcccg cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc cactgtatac
     3901 ccctgatttt atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta tcgctgaatt
     3961 ttacccgttt ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc aaaccggcga
     4021 cggccacgca atgaattgcg cccccgtcat cggttattag aggctgtctg cccccggata
     4081 cccggattct ttctgatgcc tgacggtgac gatcacaatc tggtccattt cccggtcgtg
     4141 gcggtagagc atcacatagc cactgtcgcc aaacccgatc accagttcct tatattccag
     4201 cggcagaaac ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct ggatagcccg
     4261 gacgatcacc tcacctcctt ttctggctgc cagccgattt ttggttttga gaaagtcctg
     4321 aaggcgttgt aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca tggcggagca
     4381 tcctgctcgt tctcagttcc ccaggtactt atccaggctt ccgcttcttc cgcagtaagg
     4441 tgcagacccg tttcctgata gtgctgccat gcggcctgtc cgtcccgcag gtactggctg
     4501 cgcttctctt cccggtcgat gtattcggtg attgcctcca gcattagcgc gtgcgctgag
     4561 cggtgccggt catcggccag cgttttcagc cggtccttaa gttcttcatc gagcctgatg
     4621 gatgttgctg atgacataaa agcccctttg tagtcatatg tattacacat gactacttta
     4681 atccgtggcg gcgctatttg ccagaaatac ggcttttgct gcccattttt cagcttcgct
     4741 tccactaatc agggctgcta caggctcact catcatccag gactattgca gcctgcgggt
     4801 taaaggacct gttactggcg gcaaccatca tcattgagct tttgggtggt gactggtcgc
     4861 ggatcctgta agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc
     4921 gggtgccctg cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagg
     4981 tgacagccgg taagcgacac gttgtccact cgtccatcgt ccagaatatc aaacgtggcg
     5041 gtcacctgac cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg ctgcagtgtg
     5101 gttacgggtt ttagtggtat cgcatgtacc ggagtgagga acatggccgc cagcactgtc
     5161 agagtaaaag ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt tgccaggtat
     5221 tttcatccgg tgccggcgga attttggcgt gggtaacggc tgatattgct gctttacaga
     5281 gtttcggatc tcctccttca gcaatggcgc tgttcagagt gccatcacgt tgtatggata
     5341 tccgcacggt gcacattttt cccgcatagg cctctggttc aaacatctgc atttcaacag
     5401 cggtcttgat ggccgcagcg tagctactga ttttagatga cttatccaga gtataaattt
     5461 tcggcggctg ggtggtggaa tgcttacagg ccgtcaggag taaggggctg cacaatgcca
     5521 gcaggataaa gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa atgttacatg
     5581 tatatttgtc actctttacg gccacgttca tttacataaa cacggcttat cgtatcgttt
     5641 ttacaccaaa cggcccgcca tgcagttgcg tgcataggca tgcagcgggt tacaatttac
     5701 cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg
     5761 gaaacgccct cagaccgtct gagcggtgcg ggcaatgcga cgttatggta aatacccctt
     5821 accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac
     5881 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     5941 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     6001 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     6061 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     6121 atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt
     6181 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt
     6241 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     6301 aacgggagga gtaaggtaat aattatactg tgtatatgta cagtgtgcgc cgggagaccg
     6361 gttagagatc aaggggtgaa agtccccgac cattgaagga ccagcaatcc acaaggtccc
     6421 cgagtcatgc gttgcatacc gcgaggtatg gggcgaagcg ttgacagggg tgttgacagg
     6481 ccagccattg agccacgaaa tgtatattaa attaccgggt gccgacgttg tactgttaac
     6541 ggaaggcaac atcatagggt gcgacactgc gagtgccaca tggacccggc ggggtctgag
     6601 accctggcat gtcaatacga tctctacgcg ggaaccggga gatctcccct ctgaccatct
     6661 gccagtgtcg gagatggccc gcaccgggaa gacgaggagt catagccggt gatgtacgga
     6721 gaggagaagt cggactcgct catagtagcg gcgaagcagg cgaacaaccc gaaaggagcg
     6781 gagtcagtgg agcgaaggag cggggccaag gggaacgcgg aacagccaca catgcgccgg
     6841 acacagagcc gggaaagcat gtcacagagg ctgtcacgcg tgcgggaagc tgcgaagcag
     6901 cggaagaaag aacggtttac agcattgttc cacctgctga cagtcgaagc actggaagcc
     6961 gcattcctct ccctgagcag gaaagcggcc gccggagtgg atggcatcag gtggatggac
     7021 tacgccggaa acatgaagaa caacataaca gatctgcacc ggaggctaca tcagggcagc
     7081 tacagggcgc agcccggcag gcgtcactac atcccaaaag cggatggaaa acaacgcccg
     7141 ctcggcatcg cctcgctgga ggacaagatc gtccagtatg cgctggtgaa aatcctgaac
     7201 gcagtctatg aaaacgactt tatggggttc tcatacgggt tcagacccgg gcgaagccag
     7261 cacgatgcac tggacgcact ggccacaggg ctggtacgca ctaacgtaaa ctgggtactg
     7321 gatgccgaca tcagtcagtt cttcgacagg gtgagccacg aatggctgat caggttcaca
     7381 gagcatcgga tcggcgaccg gagggtaatc aggctcatac gtaagtggct cacagccggg
     7441 acgtcggagg agggtcaatg gcgagcaacg gaggaaggca ccccacaggg tgcggtcatc
     7501 tcaccgctgc tggcaaacat atacctccac tacgtcttcg atctgtgggc gcatcagtgg
     7561 cgacgtcgct atgccacagg caatgtggta atggtcagat acgccgatga catcgtcatc
     7621 gggttcgaca aacgatacga tgcccggcgc ttccgtatag ccatgcagcg cagactgagg
     7681 gagttcggac tcacggttca cccggagaaa acccgtctga tggagttcgg ccgcttcgct
     7741 gccgaaaacc gtgccatcag gggaaaaggc aaaccagaaa cgttcaactt cctcgggttc
     7801 acgcacatca gcgggaaaga tcgcaacggc aggttcatgc tgatacgaaa gacccgccgg
     7861 gatcggatga cggcaactct gaaagccatc aaagacggtc tgcgaaggcg ctggcattac
     7921 tcaatccccg aacagggaaa atggctcagg agagtggttc agggatacct gaactatcac
     7981 tcggtaccgg gcaacttccc caccatgcag aagttcagga cacacgtaac aaacctctgg
     8041 cgccgggcgc tcaggcgcag gagccagaag gatgatacga cctggacgaa agcaaacaaa
     8101 ctggcagccg catggctacc aagggttcgg gttcttcatc catggcctgt ggagcggttc
     8161 accgccagac acccgaggca ggagcccggt gcgtaaatcg cgcacgccgg gatctgtgcg
     8221 gggggtatcc ggtaacgggt atccctaccg cgacatgttg gctttaattt attgttttaa
     8281 atgtcaaaga ggaaaacggt atgagtggtg gagacggtcg aggtccgggt aattcaggtc
     8341 tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt aaaggtggcc
     8401 cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg acgcatacgc
     8461 cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg agtaaacccg
     8521 gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggcgga cagtcttcgg
     8581 ccactgcgat ggccttcggt ctgcctgccc tggctactcc gggggctgaa ggactggctt
     8641 tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga tgtgctggct gccctgaagg
     8701 ggccgtttaa gtttggtctg tgggggattg cgatctacgg tgtgctgcct tctgagattg
     8761 caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga
     8821 cggagactcc ggcaagtact ttaccactgg accaggcgac ggttcgtgtc agacaacggg
     8881 ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcaggt cggccaatga
     8941 gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc
     9001 cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc
     9061 caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttaca gccggtggta
     9121 actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg gtttatgtgt
     9181 cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc
     9241 gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagac tatgataaag
     9301 cgaaagctga gctggatgcc gaagataaaa atattgcgac cttaaacagc cgcattgcat
     9361 cgacagagaa ggcgctcccc ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa
     9421 aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat gaatatggct
     9481 ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag aatgagaaat
     9541 taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc cttatccaga gataaggctg
     9601 cacttaccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa
     9661 ataagttaaa tgaggaaaag aacaagcctc gcaaggggac taaagactac ggccatgatt
     9721 atcatccagt acctaaaaca gaagacataa agggacttgg agagttaaag cgaggtgatc
     9781 ctaaaacacc taaacaaggt ggtggtggta agcgggcccg atggtatggc gataaaaaac
     9841 gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtatcgt gccagtgatg
     9901 gcgaacacct aggcgcattt gatcccaaaa cgggtaagca ggttaaaggg ccagatccaa
     9961 aacggaacat taaaaaatat ctttgagagg taaatatggg gcttaaatta cgtttgacat
    10021 ggtttgataa gaaaactgaa gagtttaaag gggaagagta ttctaaagac tttggtgacg
    10081 atggttctgt cattgaaagc ttgggaatgc ctttaaagga taatattaat aatggtgagt
    10141 ttgatttgaa gaaagaatgg gttcctttac tacagccatt ttttaaaaat caaataaaac
    10201 ctgatgtgga taattgttat atagcttttg attatcggga cggtaactgg taacaaaaaa
    10261 ttatcaagga tgatttgaaa tactgacttc cggtgttcag ggatgaatgc cggagtcttt
    10321 aaatacaggg aaagtatatg aaaaaggtaa agactatttt tctttttata ttgattgttg
    10381 ctggctttct tctggccgca tgtcaggcaa attatatccg ggatgttcag ggcggaacgg
    10441 tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg
    10501 atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac
    10561 ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac cgtggtaact
    10621 ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac tttggcgcac
    10681 acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc agttaccaat
    10741 ggctgctgcc agtggcgttt tgcgtgcttt cccgggttgg actcaagtga acagttaccg
    10801 gataaggcgc agcagtcggg ctgaacgggg agttcttgct tacagcccag cttggagcga
    10861 acgacctaca ccgagccgag ataccagtgt gtgagctatg agaaagcgcc acacttcccg
    10921 caagggagaa cggcggacag gtatccggta aacggcaggg tcggaacagg agagcgcaag
    10981 agggagcgac ctaccggaaa cggtggggat ctttaagtcc tgtcgggttt cgcccgtact
    11041 gtcagattca tggttgagcc tcacggctcc cacagatgca ccgaaaaagc gtctgtttat
    11101 gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg ccctgccgtt
    11161 ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc
    11221 cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca
    11281 atttccccgg cacctgcaag acggtcaccg gacgcgctga tgtgggatat tgtcccctga
    11341 ttaagtcttt ctattccctg acgatcaatt atcaggatag gtgaaccatg tcggtttttc
    11401 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga gaaatgcaag
    11461 tcatccccgc tggggcgtgg ctgacttcag gggaaaagac cttgcttacg ctggctggac
    11521 gccaccgacg cacggtcttt tcccctcttt ctggctctgt ccggctggac gccggccacg
    11581 ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa atccacggtg
    11641 ctggacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt cagccgctcg
    11701 ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt
    11761 ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg
    11821 ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc
    11881 cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg
    11941 taaacccgtc gtctgaagga gagagattgc
//
