LOCUS       NZ_CP107332            11940 bp    DNA     linear   CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_39 plasmid unnamed5.
ACCESSION   NZ_CP107332
VERSION     NZ_CP107332.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207068
            Assembly: GCF_028219975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:24:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,016
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,714
            CDSs (with protein)               :: 5,714
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 68 of 180
            Pseudo Genes (incomplete)         :: 120 of 180
            Pseudo Genes (internal stop)      :: 24 of 180
            Pseudo Genes (multiple problems)  :: 32 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_39"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            269..514
                     /locus_tag="OH536_RS29900"
     CDS             269..514
                     /locus_tag="OH536_RS29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            788..1159
                     /gene="tolA"
                     /locus_tag="OH536_RS29905"
     CDS             788..1159
                     /gene="tolA"
                     /locus_tag="OH536_RS29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1156..1521
                     /locus_tag="OH536_RS29910"
     CDS             1156..1521
                     /locus_tag="OH536_RS29910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1696..1974
                     /locus_tag="OH536_RS29915"
     CDS             1696..1974
                     /locus_tag="OH536_RS29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1962..2276
                     /locus_tag="OH536_RS29920"
     CDS             1962..2276
                     /locus_tag="OH536_RS29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2440..2868
                     /locus_tag="OH536_RS29925"
     CDS             2440..2868
                     /locus_tag="OH536_RS29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2894..3073
                     /locus_tag="OH536_RS29930"
     CDS             2894..3073
                     /locus_tag="OH536_RS29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3100..3630)
                     /locus_tag="OH536_RS29935"
     CDS             complement(3100..3630)
                     /locus_tag="OH536_RS29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3637..4368)
                     /gene="mobC"
                     /locus_tag="OH536_RS29940"
     CDS             complement(3637..4368)
                     /gene="mobC"
                     /locus_tag="OH536_RS29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4368..6332)
                     /locus_tag="OH536_RS29945"
     CDS             complement(4368..6332)
                     /locus_tag="OH536_RS29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7816..7965)
                     /locus_tag="OH536_RS29950"
     CDS             complement(7816..7965)
                     /locus_tag="OH536_RS29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8050..8307)
                     /locus_tag="OH536_RS29955"
     CDS             complement(8050..8307)
                     /locus_tag="OH536_RS29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8317..10002)
                     /locus_tag="OH536_RS29960"
     CDS             complement(8317..10002)
                     /locus_tag="OH536_RS29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(10107..11594)
                     /gene="ltrA"
                     /locus_tag="OH536_RS29965"
     CDS             complement(10107..11594)
                     /gene="ltrA"
                     /locus_tag="OH536_RS29965"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             3317..11940
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7135..7713
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3641..4368)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cacagtataa ttattacctt actcctcccg ttttcaggtg tcaatatatg agattagttc
       61 tcatcttcag gcacaaaaaa gcgcccggag ccacctgtaa gcagatcgcg tcttttcccg
      121 aaaatttctc atggcaacaa attttattat ttaagacatt catcacactt acatttcaga
      181 tggtatagac acatctgaaa gctaactggc atgaggagag gacctgcggt taaactgttc
      241 gggaaacctg agacgcccgg aggccactat gacgcacctg aaactgactc ccgctgaaga
      301 aaagcgcctg gcggacgcct ttgtcgctca ggcactggcc atgggcctga aagggaacaa
      361 aacgctgcca aaggcggtaa aacaacaggt gcgggaaagg gtgcttcgtg ccattcagga
      421 cgaacgtgaa gccaggcggg cgcaggaaat gcgtcgggag aaatacgagg ctacggaaac
      481 caccttcacc tggcagccac agcgccgccg gtaaggggta tttaccataa cgtcgcattg
      541 cccgcaccgc tcagacggtc tgagggcgtt tccggcggct acgggataaa acaggcaaca
      601 aaccacccgg aaaaccgccg gaaacgcgcc cggtaaattg taacccgctg catgcctatg
      661 cacgcaactg catggcgggc cgtttggtgt aaaaacgata cgataagccg tgtttatgta
      721 aatgaacgtg gccgtaaaga gtgacaaata tacatgtaac attttttact gacaggtcat
      781 caacccgatg cgtttaatac cctttatcct gctggcattg tgcagcccct tactcctgac
      841 ggcctgtaag cattccacca cccagccgcc gaaaatttat actctggata agtcatctaa
      901 aatcagtagc tacgctgcgg ccatcaagac cgctgttgaa atgcagatgt ttgaaccaga
      961 ggcctatgcg ggaaaaatgt gcaccgtgcg gatatccata caacgtgatg gcactctgaa
     1021 cagcgccatt gctgaaggag gagatccgaa actctgtaaa gcagcaatat cagccgttac
     1081 ccacgccaaa attccgccgg caccggatga aaatacctgg caaatattca aaaacgcccc
     1141 tctggatttc agaccatgaa agcttttact ctgacagtgc tggcggccat gttcctcact
     1201 ccggtacatg cgataccact aaaacccgta accacactgc agccaaccta cccggtacag
     1261 gccgcagcaa aacgcatcga aggtcaggtg accgccacgt ttgatattct ggacgatgga
     1321 cgagtggaca acgtgtcgct taccggctgt cacctttttt ttcgggaaac gctgacggca
     1381 atgaggcact ggctatatcc cgcagggcac ccggcaatgc gacaggttat aaattttgaa
     1441 ttcagtctga aaaatgacgg cttacaggat ccgcgaccag tcaccaccca aaagctcaat
     1501 gatgatggtt gccgccagta acaggtcctt taacccgcag gctgcaatag tcctggatga
     1561 tgagtgagcc tgtagcagcc ctgattagtg gaagcgaagc tgaaaaatgg gcagcaaaag
     1621 ccgtatttct ggcaaatagc gccgccacgg attaaagtag tcatgtgtaa tacatatgac
     1681 tacaaagggg cttttatgtc atcagcaaca tccatcaggc tcgatgaaga acttaaggac
     1741 cggctgaaaa cgctggccga tgaccggcac cgctcagcgc acgcgctaat gctggaggca
     1801 atcaccgaat acatcgaccg ggaagagaag cgcagccagt acctgcggga cggacaggcc
     1861 gcatggcagc actatcagga aacgggtctg caccttactg cggaagaagc ggaagcctgg
     1921 ataagtacct ggggaactga gaacgagcag gatgctccgc catgccacag gtaacgattt
     1981 ccgcgctggc acagcgcgat ttacaacgcc ttcaggactt tctcaaaacc aaaaatcggc
     2041 tggcagccag aaaaggaggt gaggtgatcg tccgggctat ccagcaactg aaaacattgc
     2101 cagacattgg ccgcccggtg ccgtttctgc cgctggaata taaggaactg gtgatcgggt
     2161 ttggcgacag tggctatgtg atgctctacc gccacgaccg ggaaatggac cagattgtga
     2221 tcgtcaccgt caggcatcag aaagaatccg ggtatccggg ggcagacagc ctctaataac
     2281 cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt ttgacaagcg cagcgcgtca
     2341 gaaaaccaca tcctgaacgg aaaaacgggt aaaattcagc gatacattta ccgtgagaaa
     2401 ttacacacct ttcacacccg ataaaatcag gggtatacag tggcgaaaac attgctcaac
     2461 ctttcacagg cagcacaagc cgcgggcatt accagaagaa cgctatacaa tcatgtaaaa
     2521 cagggaaagg ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga tgtgtcagaa
     2581 ctgatccgtg tatacggaaa tgtgaatatc cctgagaaac aaattcccgg catttcacac
     2641 cgtgaaaata cacaaaaaaa tttcccacag gaacagcttc tggcaatgca aaaggaactg
     2701 gccgatctcc ggcaggctgt caccctgatg ctggaagata aaacatcccg ggaagaagaa
     2761 cgccgccagc acgatgatga gcgccggaaa ctccaggccg aagtggacag attgacaaca
     2821 gaactgacac agaagaaaaa acgcttctgg tccggctggt ttagctgaga acttaccgaa
     2881 gggaatggag aacatgacga agcaggaaaa aactgccctc aatatggcta aattcattaa
     2941 ggatcagtcg ctgctgttgc tggaaaagct gaacgagctg gacatggata ccccggccga
     3001 catgtgcgaa cggcttcacg aggatgcgga aacactctgg caggtgatgc gggataagct
     3061 cggggaagaa taacagcacc ggccatgccg gtgctggcgt tattttttgc cgaacggcca
     3121 ccaccggcgg gcgggtttgt cctccggcag ttcaggcacc ggcacgttaa aatagcggct
     3181 gttatattcc cgcatcagct ccgctttcag cttctcgttc accgcgcttt ccccgtgccg
     3241 ctcgctgacc acctcaatca tcgcgttaat ctgctccggt gagcacttcc cggcatcaac
     3301 cacctgcaca ccgttctcga tggcgaaccg ctccaccttt cgatagaggt aatcacacag
     3361 cttcattcct gtgggtttgt cccgttccca ttgcggattt tcaccggtga aaaagtacgt
     3421 ggctttttcg tcgctgttga gccgctcgtc atacatcccg atggatttca tcatcgccag
     3481 atgctgggcc cggtcgacaa aacagaaaac atacgccccg gtctgccagg cgagcaccac
     3541 catgtcttcc agatgctcgc tggttggcat aatgcagcgc tcgccgtctt tgtcttccac
     3601 gtcctcaata aataccatgt acagcgccat aatccctcac tccgttgtca taaatttaaa
     3661 gcacgcgaac agcctctccc ggtccagctc caccgttttg cgggcctcgc cggtctgtgg
     3721 attcacgatt tcatccaccg ccagaatcgc ccgctgtacc cggacaagcg tcggccggtc
     3781 agggcagatg taccagattt catcgatgcc gttcgcccgg cggttgaaca gatacccgga
     3841 caccacttcc tgataccgcc tgcggctctt gacggtgcgc tctacctcaa atgccaccgt
     3901 tctgccgtcc agctccgcca gcgcgtccgg cactttcatc cctttcaggg ccatacggtg
     3961 caggtaccgc cagccggtgg ccccccggcc ctccatggag aggcgcaggg actgcacggc
     4021 cagccggtgc ggcagctgcg ccgccgacac ccttgacggc tgaaacggaa taacgtccgt
     4081 cagcggctcg tcctcgtcga acgagaacgc caccccgtgc ggcgtcagcc cccacacgct
     4141 gacctgacgg ccgtcaaccg ggtatttcgc ccggaccagc agccctttcg ccaccagact
     4201 gtccaggaag cggtaaaccg gctgtaccgc cgcaatcccc agccgtcgcg caaccagctc
     4261 cgccgtggaa cacagctctt cccgcagcca gcgcagggtc agccgtgttt tctccgcgtt
     4321 ccgcgcctgc cgcttcgcgt ggctgacgtt gtactgctcc agtgtcatca gtaaagcccc
     4381 gcaaaatcat catccgcttt tggtccgccg ccgtcggccg gaggcaggta ctccgccagc
     4441 gggtccgttt tcgccagcac cgggaaggtt ttcagcgaaa tttcccggcg ctcaacccgc
     4501 agcgggctgc tgaacgccag ccgcgccacg cccagcccgg tcatcaccgc gaacccgtca
     4561 ggcaggtgtt gcagggtgtt cacgtcaaac agcggccgcg cctccttctg gatgaagctg
     4621 tcgccgctga tgtgctccac gttccccgcc tcccggctgg cccggcggcg ctccacgtcc
     4681 acccggatct ccccggtctg gcccgccgcc cactgcgcgg actcctggct ggccgcgcga
     4741 tagaaccagc ggatcggcgt gttatccagt accgtggttt tcacaaaatc agcgggcaga
     4801 tcctgcccgc actgaccgaa gtcgcccagc gactggtgcg cgagcagcag gttgcagtcc
     4861 cggctgcgca gcgtccccag cgcgttcagc acgtacttcg acagcagata tttaatctca
     4921 tcgagcatga tgctcgcgtg cggccacctc cggaactcat cccgcgcaat gatgatttgc
     4981 gcgcagcggg caaacagcat cttctgcacc cggatcaccg cctcatcgtc catcgagccg
     5041 atgacgtaca ggcagccgcc gccgttgatt atccccgcca catcgatacc gctgtccgtc
     5101 tgcaggacgc tcaggtcggc cacgttctcc agcttggtga tcagcccctt cacgtccttt
     5161 ttcagggcct ccggcaacgc gtacgccgcc gccagcacct gctgaatgtt tgccccctgc
     5221 gggaactgct ccgcaatcag cttcgccgct ttctgctcgc tgatgcggta gtggtccgcc
     5281 acgtcgccgg tctcactcag gttaaacccg gcaaccagca gattcttcag ggcgtactgg
     5341 tcgagatcgc ggaacagatt cagctgcgcc ggtttccccg gccgcagatc cagcagggtg
     5401 aagtccgggc agtgcgcccg gaagacgtgc ggcgcccacg cgtccccttt ggggtcgaag
     5461 tacaccacag cgtccccgaa atcgcgtacg cagcgaatga gcgcgttggt ggccatcgcg
     5521 ctcttgccgc tcccgggcag gccgaggatc tgaatattgg tcttgcgcca ggtatcacga
     5581 gggaccagca ccggctggcc ctgctcatcc ttacccagaa acaccgcctg ctgtttccgg
     5641 gcctgctccg tggaaaaatc ggccagcgcg ttaaccggta ccggctcccc gaaatgcagg
     5701 tggcggacgt ctgtcatttc tccggtccgc tggctgctgc gccggctctc ctgattcagt
     5761 ttcgcgttca ccgcctcgtc agcggccgca aaatgaccat caagcagcag gaacgccagc
     5821 agccgcgcca gaaggacgcc ggcgacaagc cctccggcac accaggctat cccgaacagg
     5881 ttagccggct gcagcaggta ataatctgcg ttcgcataga ggtccgggcg caggacggtt
     5941 tctccccagc tctcgataaa cacggcgaca accagccccc ccagaagggc cagaccggca
     6001 gcgccggtca gccagtacgc gtagttccag acatgtcgcc gtgccctgac catccgcccg
     6061 ggtgaatcca gcgaggccag caccgaaaat ggcacagtcc ggttattatc gcgcttatag
     6121 cgatcggcat actcccgccg cgccttatgg ctcagcccca caaaacgcat tcggccggcc
     6181 cagagttcag ccagaccgaa aaacaggccg ataaccagaa aaaaaccggc cagactgtaa
     6241 tactcccggt acagccagct gacccacggg taaatctgcc gggccagctc cgggatatgc
     6301 cactgccagg caagcgaatc cgtattaaac atgcaatctc tctccttcag acgacgggtt
     6361 taccggcgtg atgcgcagcc cccgctgcac aagccgcgga catgccccaa ggcatggcgg
     6421 cggcggtcac gtccgtggtg attgcgttca gctttcaccg cggaggccag cctctttccg
     6481 ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc gtgtgagagg
     6541 aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag agttcgccat
     6601 agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgtc aggtgcgtcc
     6661 agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct
     6721 ggcgtggccg gcgtccagcc ggacagagcc agaaagaggg gaaaagaccg tgcgtcggtg
     6781 gcgtccagcc agcgtaagca aggtcttttc ccctgaagtc agccacgccc cagcggggat
     6841 gacttgcatt tctccgcaag gagaaaaaag caacagagat aggttaacgt aaaatgttca
     6901 gcgaaaaacc gacatggttc acctatcctg ataattgatc gtcagggaat agaaagactt
     6961 aatcagggga caatatccca catcagcgcg tccggtgacc gtcttgcagg tgccggggaa
     7021 attgcgcctc ctcgctcact cggtcgctac gctccgggcg tgagactgcg gcgggcgctg
     7081 cggacacata caaagttacc cacagattcc gtggataagc aggggactaa catgtgaggc
     7141 aaaacggcag ggccgcgccg gtggcgtttt tccataggct ccgccctcct gccagagttc
     7201 acataaacag acgctttttc ggtgcatctg tgggagccgt gaggctcaac catgaatctg
     7261 acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggt aggtcgctcc
     7321 ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgtccgc cgttctccct
     7381 tgcgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc ggtgtaggtc
     7441 gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta
     7501 tccggtaact gttcacttga gtccaacccg ggaaagcacg caaaacgcca ctggcagcag
     7561 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa
     7621 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
     7681 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
     7741 aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag
     7801 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc
     7861 caccgttccg ccctgaacat cccggatata atttgcctga catgcggcca gaagaaagcc
     7921 agcaacaatc aatataaaaa gaaaaatagt ctttaccttt ttcatatact ttccctgtat
     7981 ttaaagactc cggcattcat ccctgaacac cggaagtcag tatttcaaat catccttgat
     8041 aattttttgt taccagttac cgtcccgata atcaaaagct atataacaat tatccacatc
     8101 aggttttatt tgatttttaa aaaatggctg tagtaaagga acccattctt tcttcaaatc
     8161 aaactcacca ttattaatat tatcctttaa aggcattccc aagctttcaa tgacagaacc
     8221 atcgtcacca aagtctttag aatactcttc ccctttaaac tcttcagttt tcttatcaaa
     8281 ccatgtcaaa cgtaatttaa gccccatatt tacctctcaa agatattttt taatgttccg
     8341 ttttggatct ggccctttaa cctgcttacc cgttttggga tcaaatgcgc ctaggtgttc
     8401 gccatcactg gcacgatacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     8461 acgtttttta tcgccatacc atcgggcccg cttaccacca ccaccttgtt taggtgtttt
     8521 aggatcacct cgctttaact ctccaagtcc ctttatgtct tctgttttag gtactggatg
     8581 ataatcatgg ccgtagtctt tagtcccctt gcgaggcttg ttcttttcct catttaactt
     8641 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggtaag
     8701 tgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct gagccgcttt
     8761 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     8821 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     8881 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggagcg ccttctctgt
     8941 cgatgcaatg cggctgttta aggtcgcaat atttttatct tcggcatcca gctcagcttt
     9001 cgctttatca tagtctcttt tcagcccctc ttccggatga gcggcgtccc atgcctgctg
     9061 gcgacgcttt tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa catctgtcac
     9121 cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga
     9181 gttaccaccg gctgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt
     9241 tggcggggct ttcgctgtcg gaacaccttt cggtacgctc acctgcagag acgggagacc
     9301 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacagggac
     9361 actcattggc cgacctgcga caaccgcaat atgctgccgc tcatccttca ccacatccac
     9421 aacccgttgt ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg ccggagtctc
     9481 cgtcaccgca tcggccggta atgacgtcat aatttttgac atcattttcg gatcatcttt
     9541 tgcaatctca gaaggcagca caccgtagat cgcaatcccc cacagaccaa acttaaacgg
     9601 ccccttcagg gcagccagca catcagcaac ggcggccgac aacgcatcgc cggaaactga
     9661 taaagccagt ccttcagccc ccggagtagc cagggcaggc agaccgaagg ccatcgcagt
     9721 ggccgaagac tgtccgccac cagagctacc gctatgattg ccgctgttac caccattacc
     9781 accgggttta ctcccgccgt taccaaactc ccccggatta tagttatgaa tatctccatt
     9841 cggcgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     9901 agggccacct ttaccagacg taccgttcac attcccactg gcctgaccac cattatgtcc
     9961 cagacctgaa ttacccggac ctcgaccgtc tccaccactc ataccgtttt cctctttgac
    10021 atttaaaaca ataaattaaa gccaacatgt cgcggtaggg atacccgtta ccggataccc
    10081 cccgcacaga tcccggcgtg cgcgatttac gcaccgggct cctgcctcgg gtgtctggcg
    10141 gtgaaccgct ccacaggcca tggatgaaga acccgaaccc ttggtagcca tgcggctgcc
    10201 agtttgtttg ctttcgtcca ggtcgtatca tccttctggc tcctgcgcct gagcgcccgg
    10261 cgccagaggt ttgttacgtg tgtcctgaac ttctgcatgg tggggaagtt gcccggtacc
    10321 gagtgatagt tcaggtatcc ctgaaccact ctcctgagcc attttccctg ttcggggatt
    10381 gagtaatgcc agcgccttcg cagaccgtct ttgatggctt tcagagttgc cgtcatccga
    10441 tcccggcggg tctttcgtat cagcatgaac ctgccgttgc gatctttccc gctgatgtgc
    10501 gtgaacccga ggaagttgaa cgtttctggt ttgccttttc ccctgatggc acggttttcg
    10561 gcagcgaagc ggccgaactc catcagacgg gttttctccg ggtgaaccgt gagtccgaac
    10621 tccctcagtc tgcgctgcat ggctatacgg aagcgccggg catcgtatcg tttgtcgaac
    10681 ccgatgacga tgtcatcggc gtatctgacc attaccacat tgcctgtggc atagcgacgt
    10741 cgccactgat gcgcccacag atcgaagacg tagtggaggt atatgtttgc cagcagcggt
    10801 gagatgaccg caccctgtgg ggtgccttcc tccgttgctc gccattgacc ctcctccgac
    10861 gtcccggctg tgagccactt acgtatgagc ctgattaccc tccggtcgcc gatccgatgc
    10921 tctgtgaacc tgatcagcca ttcgtggctc accctgtcga agaactgact gatgtcggca
    10981 tccagtaccc agtttacgtt agtgcgtacc agccctgtgg ccagtgcgtc cagtgcatcg
    11041 tgctggcttc gcccgggtct gaacccgtat gagaacccca taaagtcgtt ttcatagact
    11101 gcgttcagga ttttcaccag cgcatactgg acgatcttgt cctccagcga ggcgatgccg
    11161 agcgggcgtt gttttccatc cgcttttggg atgtagtgac gcctgccggg ctgcgccctg
    11221 tagctgccct gatgtagcct ccggtgcaga tctgttatgt tgttcttcat gtttccggcg
    11281 tagtccatcc acctgatgcc atccactccg gcggccgctt tcctgctcag ggagaggaat
    11341 gcggcttcca gtgcttcgac tgtcagcagg tggaacaatg ctgtaaaccg ttctttcttc
    11401 cgctgcttcg cagcttcccg cacgcgtgac agcctctgtg acatgctttc ccggctctgt
    11461 gtccggcgca tgtgtggctg ttccgcgttc cccttggccc cgctccttcg ctccactgac
    11521 tccgctcctt tcgggttgtt cgcctgcttc gccgctacta tgagcgagtc cgacttctcc
    11581 tctccgtaca tcaccggcta tgactcctcg tcttcccggt gcgggccatc tccgacactg
    11641 gcagatggtc agaggggaga tctcccggtt cccgcgtaga gatcgtattg acatgccagg
    11701 gtctcagacc ccgccgggtc catgtggcac tcgcagtatc gcaccctatg atgttgcctt
    11761 ccgttaacag tacaacgtcg gcacccggta atttaatata catttcgtgg ctcaatggct
    11821 ggcctgtcaa cacccctgtc aacgcttcgc cccatacctc gcggtatgca acgcatgact
    11881 cggggacctt gtggattgct ggtccttcaa tggtcgggga ctttcacccc ttgatctcta
//
