LOCUS       NZ_CP107326             5596 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRKP_40 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP107326
VERSION     NZ_CP107326.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31207069
            Assembly: GCF_028219955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hu,F., Lin,M.L., Mou,J.L., Feng,J.H., Huang,K., Lao,Y.J., Cheng,J.
            and Lin,J.
  TITLE     Molecular and Clinical Characteristics of Carbapenem-Resistant
            Klebsiella pneumoniae Isolates at a Tertiary Hospital in Wuhan,
            China
  JOURNAL   Infect Drug Resist 16, 65-76 (2023)
   PUBMED   36636376
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2022) Department of Gastroenterology/Hepatology,
            Zhongnan Hospital of Wuhan University, Donghu Street, Wuhan, Hubei
            430071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300X
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:33:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,016
            CDSs (total)                      :: 5,894
            Genes (coding)                    :: 5,713
            CDSs (with protein)               :: 5,713
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 69 of 181
            Pseudo Genes (incomplete)         :: 120 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 32 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107326.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRKP_40"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China: Wuhan"
                     /collection_date="2018-12-04"
                     /collected_by="Hu Fan"
     gene            1..225
                     /locus_tag="OH527_RS29970"
     CDS             1..225
                     /locus_tag="OH527_RS29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            209..475
                     /locus_tag="OH527_RS29975"
     CDS             209..475
                     /locus_tag="OH527_RS29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..895)
                     /locus_tag="OH527_RS29980"
     CDS             complement(545..895)
                     /locus_tag="OH527_RS29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(961..1506)
                     /locus_tag="OH527_RS29985"
     CDS             complement(961..1506)
                     /locus_tag="OH527_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1532..1858)
                     /locus_tag="OH527_RS29990"
     CDS             complement(1532..1858)
                     /locus_tag="OH527_RS29990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2216..2374)
                     /locus_tag="OH527_RS30110"
     CDS             complement(2216..2374)
                     /locus_tag="OH527_RS30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3014..3190
                     /locus_tag="OH527_RS29995"
     CDS             3014..3190
                     /locus_tag="OH527_RS29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3180..3524
                     /locus_tag="OH527_RS30000"
     CDS             3180..3524
                     /locus_tag="OH527_RS30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3733..>4167
                     /locus_tag="OH527_RS30005"
                     /pseudo=""
     CDS             3733..>4167
                     /locus_tag="OH527_RS30005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4202..4711
                     /locus_tag="OH527_RS30010"
     CDS             4202..4711
                     /locus_tag="OH527_RS30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4718..4930
                     /locus_tag="OH527_RS30015"
     CDS             4718..4930
                     /locus_tag="OH527_RS30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2849..2928
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2266..2663)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3515..5046
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcttgcta tccggttatc tgatgagatt gagtcccgtc tggactcgct ggcgaagcaa
       61 accggcagaa caaagacgtt ttatgcgcgg gaagcaatac tggcgcatct ggaagacctg
      121 gaggattatt atctttcagc agaaactgct gcacgcgttc gccgtggtga tgaagcagtg
      181 cattcgtctg aagacgtgag gaagtcactt ggtctggacg attaactatt ccgatcgggc
      241 gctcaaatcg ttacgcaaga tggacaaaca gaacgcacga cggattgtgg attttatgag
      301 tttacgcatt gcagttgctg ccgatcctcg ccagtcaggg aagccgctca aaggtgagct
      361 gggcgagttc tggcgctatc gggtgggaga ttatcgcgtt ctgtgtgaga tccgagatga
      421 cgagcttgtt atccttgccg ccacgattgg acatcgccgc gaagtttacg actgaagccc
      481 gctatgcggg cttttttgat gccgacaaca ggtattatgg taaatactcg actaactacg
      541 tgttctactg tttgtcggtt ttagttaacc agcaggttgt cttaacgata atgtagccgt
      601 tatttttaac taataaggtc atattgttaa cgtcaggcat aataaaagac attatggcct
      661 cagagttatc atcagaagca gctctctgca ttactacttc gattcccttt tctttgaatc
      721 tataagtatc cagttgccta ttaaacttag aaagacctac aaccatactt agtttgttat
      781 ctttaacagt aagagatgct tcctgagtac cattacatcg gtagtaagcg gtgtaaccgt
      841 cagcaaaaga tcgaccgggg aatataaatg ctaggaacgc aattaaccaa tacatagttg
      901 agaatcttgg tattacacat ttcactatcc tctctttcat ttttcgttca ccatactttg
      961 ttatctgatt tttaattttt ctattttata aaccaagtct ctaccaaaaa ggcgaaaatt
     1021 atcctcaaat aataataatt gtttaagaac atccatgaaa gcagctgctt caccctcagt
     1081 tatgggagct actctatata acatataacc tgagttaagg gtttctttta tactttcttc
     1141 aatgctcatt ataaatatta gtggattatc atttttcttc ccccatgggt ttctaattaa
     1201 tccttcgatg tactcaggtc ctttgcgtcc tattgcctgc gcaatatttt ttatttcatc
     1261 tgcgtgaggg ctttgttgta cagctttgtt tatgcctgct tgagtgattt tcagagactc
     1321 tacaccgtgt atgttgtatg agataaaatg taataaatta agatgcaatg caattaatcc
     1381 atttttgttt acatctgagt atttggataa aaaacgtaat gaagtgttaa ctctcccttg
     1441 aagcctaaca attgggagtg ctgccttgta tatttccttc tccgttaaat accgtttaaa
     1501 aaacataatt ctccgcctcc gtatagcgtg cctattttat ttcgtcgtaa aacttagctc
     1561 tgaaatattc ctttagaacc atcatgtcat taataattga atctatctca tccatttttt
     1621 cttggtattc ttttaatggt gaagggagcg gtaactcaat atttgaaaag tgattggcga
     1681 gatagggatc aaggtttctc atccattcag gcttttcgcc aatgcgttct tcaactgaaa
     1741 gtgaacctga taatctctgg ttaataagat cacttagact cattttggtt ttattcgaaa
     1801 gccgttgtaa ggattgcttt aatcctatat ctagcatgat atttgttctt tctctcatta
     1861 cccccccttg ctattgcgtt ttttgttgcg cacaaagcta ccattgcgca ttaagaaacg
     1921 caataaaaaa ggagggaggg agtggtttgt acatgatgtg cagtgcgctg agggcatcag
     1981 tggcgggtgt ttttccgtgg gatggatgga gctccgggga taacagaact gacgatcatt
     2041 taaaggatct tcttgagatc ctttttttct gcgcgtaatc tcttgcactg taaacgaaaa
     2101 aaccgcctag ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccggg
     2161 taactgggta tacgagaccg cagataccaa aactgttctt tcagtgtagc cgcagttagg
     2221 cacccacttc aagacctctc aatatctctc tcgtatatat ccagttacca atggctgctg
     2281 ccagtggcgt ttttgcgtgt ccgtccgggt tggactcaag atgatagtta ccggaaaggg
     2341 cgcagcagtc gggctgaacg gggggttcgt gcatacagcc cagcttggag cgaacgacct
     2401 aaaccgagcc gagataccag cagcgtgagc tatgagaaag cgccacgctt cccgaaaggg
     2461 aaaaaggcgg aacaggtatc cggtaaacgg cagggtcgga acaggagagc gcacgaggga
     2521 gccgccggag agaaatcacc ggcatcttta agtcctgtcg ggtttcgtcc tcctctgact
     2581 tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag cctatggaaa aacggcaacg
     2641 ccgcatcctt ttctccggcc ctttctgatt tgagcgcctt tgctttatcc cgttatccgt
     2701 gagtcggtca cgcccgccgc agccgaacgg caggagcgca gctaccgagt gagcgaggag
     2761 gcgtcatttc atctgtccgt gcatggtatt gcggacgctc ctgagccaca tatcacacct
     2821 gccacatgag gcactttcat gaatcgtgct ctcatgccaa catagtaagc cagtatacac
     2881 agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa cccgctaaca ccactgacgc
     2941 gctggcgctt gtccaacgcc ggagcgaccc ggaaaacttt tccgtgccgt ccggcgtttg
     3001 cgaaggaggg ataatgcaga agccgaagaa gaagccgctc gtcgtaaaaa aaagatcctg
     3061 gcatccggca gtgatggcaa atctgatgcg tcaggcacag gaggaagatt accgccggga
     3121 gcagatggca gagcttgagc gttcagcccg gtcacatttt gcggatatcg gaggagagga
     3181 tggcggttaa gcgtacccgt tttttaggta tccgggtgac cgatggggag taccagcagc
     3241 tgctggagcg ctgcaatgga aggcaactgg cggtgtggat gcgggaaacc tgtctcgata
     3301 cgcgcccggc gcggtcgttg cggctgccgt ccatcgatcc ggttttgctg cgccagcttg
     3361 ccgggatggg gaacaacctc aaccagatcg cccggaaaat taacggcggc cagtggtcag
     3421 gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat tgatgccgaa ctcgagcggc
     3481 tgcggcatgc cgtgctggaa aagggggcag acgatgatcg ttaagtttca tccgcgaggg
     3541 cgaggcggcg gtgccgggcc ggtggattat ctgctgggga aagaccgcca gcgcgacggg
     3601 gccatcgtcc tgcagggaaa gccggaggag gtccgggagc ttatcgacgc ctcgccctac
     3661 gccaaaaagt acacctccgg cgtcctgtct tttgctgaac aggatttacc gcccggccag
     3721 cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc ccggactcga taaagaccag
     3781 tacagcgtgc tgtgggttga acaccgggac aaggggcggc tggaactgaa ttttctggta
     3841 ccgaacacgg aactgctgac cggcaggcgt ctccagccgt actacgaccg ggcagaccgt
     3901 ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac tggggctgca cgacccgaac
     3961 gcaccggaga accggcgcgc gctggtgacg ccatcttcgt tacccaaaac gaagcaggaa
     4021 gccgcagagg ctattacgcg gggcttactg gccctcgcct cgtcagggga gcttaaaacg
     4081 cgtcaggacg tcactgaggc gctggaaagc gcaggttttg aggtcgtgcg caccacaaag
     4141 agcagcatca gcattgccga cccggacgga gggcgaaaca tccgacttaa gggagccatc
     4201 tatgaacagt cttttaacgc tggcgaagga cttagagcag aaatcgaaag cgcagcagca
     4261 gagtaccggc gaaatgctga aagccgcatt cagcgagcac gagaagtctg tcagagcgga
     4321 actgagcgaa agcgggaaga gaatcagcgc cgccatccgc gcccacgagc agggtatgag
     4381 cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg gtcgggcgga catggctgac
     4441 catcacgatg gtgtcaggac tgctgttcgc cagcctgagc ggggtgttgt ggtatcaggg
     4501 gagcttgata gcgtcaaatc tggccgaaat cgaccggcag aacgcagcgc tgtcgaagct
     4561 gaacgcgaag acctggggcg tgacgtacct ggaagacagc aacggacgtt ttctggtgct
     4621 gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg ggcggtcgaa
     4681 gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg aaaaacagtt
     4741 gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg agtgggagag
     4801 cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg cggcgctgag
     4861 cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac tgcagcgttt
     4921 gagccggtag tgcagcgtca tgagatggcc gtggccgcag aacgggcgca cgagcaacgg
     4981 cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc gaagctatga cggtccgtcg
     5041 ctgggctgaa atgcaggaaa acagggttct catcgggcat ttacgctgaa accggatgtc
     5101 attcacctgg ctgcatggct atgcagccag gtaaaattta tctgtcgcga acggggcggt
     5161 ttatcgggtg gtttgttgcc ggttttacct gactgccacc ccgttcgcgg cgaacccgtc
     5221 cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga tacaaaaaag
     5281 ccccggaggg cttttaacta ctgacttatt tttttgaaga tttgctgggt ttcaatttgt
     5341 cagtatttcc cggcgttttt ttattgtcac gctggcgttt atcaggttcc cctgttgaat
     5401 cggcaattcg tttcggaccc ggcttaagac ccataatcgt tatcctttca atagttaaga
     5461 ggaaagccct caagctagat atattgaccg cgttacggat tatcaagagg ggctaacaag
     5521 attatttttt gttcttttag gttgattgtg gtactgttga tatacatgta tagcaaatgg
     5581 tgttcgggag gtttat
//
