LOCUS       NZ_CP107237             8274 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain REF1 plasmid pREF1-18-3, complete sequence.
ACCESSION   NZ_CP107237
VERSION     NZ_CP107237.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31158898
            Assembly: GCF_036704625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Karami,N., Sandberg,M., Yazdanshenas,S., KK,S., Jaen-Luchoro,D.,
            Westerlund,F. and Ahren,C.
  TITLE     Comparison of blaCTX-M-15 and blaCTX-M-27 carrying plasmids by
            combining ODM and CRISPR/Cas9 from different isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Karami,N., Sandberg,M., Yazdanshenas,S., KK,S., Jaen-Luchoro,D.,
            Westerlund,F. and Ahren,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2022) Infectious Diseases, University of
            Gothenburg - CCUG, Guldhedsgatan 10a, Box 7193, Gothenburg, Vastra
            Gotaland 40234, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 437x
            Sequencing Technology :: Illumina NovaSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036704625.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 16:01:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,368
            CDSs (total)                      :: 5,250
            Genes (coding)                    :: 5,008
            CDSs (with protein)               :: 5,008
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 91 of 242
            Pseudo Genes (incomplete)         :: 170 of 242
            Pseudo Genes (internal stop)      :: 43 of 242
            Pseudo Genes (multiple problems)  :: 56 of 242
            Pseudo Genes (short protein)      :: 3 of 242
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107237.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="REF1"
                     /isolation_source="human"
                     /db_xref="taxon:562"
                     /plasmid="pREF1-18-3"
                     /geo_loc_name="Sweden: Gothenburg"
                     /collection_date="2010"
     gene            complement(join(8268..8274,1..143))
                     /locus_tag="OGY64_RS26755"
                     /old_locus_tag="OGY64_26640"
                     /pseudo=""
     CDS             complement(join(8268..8274,1..143))
                     /locus_tag="OGY64_RS26755"
                     /old_locus_tag="OGY64_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            428..751
                     /gene="mobA"
                     /locus_tag="OGY64_RS26760"
                     /old_locus_tag="OGY64_26645"
     CDS             428..751
                     /gene="mobA"
                     /locus_tag="OGY64_RS26760"
                     /old_locus_tag="OGY64_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(986..1366)
                     /gene="lsoB"
                     /locus_tag="OGY64_RS26765"
                     /old_locus_tag="OGY64_26650"
     CDS             complement(986..1366)
                     /gene="lsoB"
                     /locus_tag="OGY64_RS26765"
                     /old_locus_tag="OGY64_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(1363..2403)
                     /gene="lsoA"
                     /locus_tag="OGY64_RS26770"
                     /old_locus_tag="OGY64_26655"
     CDS             complement(1363..2403)
                     /gene="lsoA"
                     /locus_tag="OGY64_RS26770"
                     /old_locus_tag="OGY64_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            complement(2668..2781)
                     /locus_tag="OGY64_RS26775"
                     /old_locus_tag="OGY64_26660"
     CDS             complement(2668..2781)
                     /locus_tag="OGY64_RS26775"
                     /old_locus_tag="OGY64_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(3360..6365)
                     /locus_tag="OGY64_RS26780"
                     /old_locus_tag="OGY64_26665"
     CDS             complement(3360..6365)
                     /locus_tag="OGY64_RS26780"
                     /old_locus_tag="OGY64_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6529..7086
                     /locus_tag="OGY64_RS26785"
                     /old_locus_tag="OGY64_26670"
     CDS             6529..7086
                     /locus_tag="OGY64_RS26785"
                     /old_locus_tag="OGY64_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            7269..8129
                     /locus_tag="OGY64_RS26790"
                     /old_locus_tag="OGY64_26675"
     CDS             7269..8129
                     /locus_tag="OGY64_RS26790"
                     /old_locus_tag="OGY64_26675"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     ARG             7270..8126
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          179..419
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(2710..3239)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          429..748
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.19e-62"
ORIGIN
        1 ccttcttctg cgagttcgtg cagcttctca cacatggcag cctgttcgtc agcatcgagt
       61 gcgtccagct tttcgagcag cgtcaggctc tggcttttta tgaatcctgc catgttgagt
      121 acagcttgct gctgcttgtt catcttttcg ttttctccgt tctgtctgtc atctgcgttg
      181 tgtgattata tcgcgtacca cttttcgact gttttaccgc cggtattctg ccgtctgacg
      241 ctttgacggg tatttctgcc tgacaatact gtcactgcca aaaaactgcc gtgcctttgt
      301 cggtaattcg agcttgctga caggacagga tgtgcaattg ttataccgcg catacatgca
      361 cgctattaca attaccctgg tcagggcttc gccccgacac cccatgtcag atacggagcc
      421 atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt
      481 tcgtctgacg gcagaagaag accaggaaat cagaaaaagg gctgctgaat gcggcaagac
      541 cgtttctggt tttttacggg cggcagctct cggtaagaaa gttaactcac tgactgatga
      601 ccgggtgctg aaagaagtta tgcgactggg ggcgttgcag aaaaaactct ttatcgacgg
      661 caagcgtgtc ggggacagag agtatgcgga ggtgctgatc gctattacgg agtatcaccg
      721 tgccctgtta tccaggctta tggcagatta acttcccgga gagaaactgt cgaaaacaga
      781 cggtatgaac gccgtaagcc cccaaaccga tcgccattca ctttcatgca tagctatgca
      841 gtgagctgaa agcgatcctg acgcattttt ccggtttacc ccggggaaaa catctctttt
      901 tgcggtgtct gcgtcagaat cgcgttcagc gcgttttggc ggtgcgcgta atgagacgtt
      961 atggtaaatg tcttctggct tgatattata ttggaatgcc ttttttcaaa gcaaatgatg
     1021 tggctttgga tagaaggttt acgttgatct tatcaaagtt ttttttaaag aacgaagccg
     1081 agagctcaga taaatcatta tattcatcag ttttcgtaac tttgtttaat gtgtaacttg
     1141 aaaacttctc gccattaaat gacgtataga cgtaacgatc tttttttcca ccgttaggaa
     1201 ttattaaatc aaaaaaaaca tcacccttgc ttttcttttt cttcaagtcg gattcgattt
     1261 ttgagaaaaa ttcgctcggg ctataaatat cagtagcata gacaataaat aaagttttat
     1321 ctttattttt tattgcttct atttgatatt ttttatcttt tttcataatt tcaacctagc
     1381 tacgccacca tctattaatt ggcaaacggt atcgatgatt gcgattgatt ctaatttgtt
     1441 aattgtcttc gtgtcagcta ttcctggttt catatgaaac aaaccatgcc tgttctcatg
     1501 ccagtaagtg tagcattcac acaaaacttc cgctatttca ccatttatag tttcttggtg
     1561 tatttctctg attatatatt tgggtttgtt ttcagtgaag tattcgccaa ggttctttga
     1621 tgatgatgga ttgcaaacat cattaagtat ttgatatata aagccttcta tggctcttaa
     1681 tgcagagaag cagtatgttg agtaatcttc catttcgaca tctatttttt tcattattag
     1741 cgagcatgat agctgttttt tgatatcttc atggatttta tcgatgcttt ttggtagttt
     1801 gctatgcaac tcggactcaa tagtttcttt ttttatgtca acattaaatt ctttattttt
     1861 ttgttcgaca atctctttca tgtttagtat tgagcacatg aaatcgttaa tcaaactcgc
     1921 gatttgaagg tattttcctt ggaattgaat agagccgcgc ttgtaaattt ttgccctgac
     1981 cctgtcacca ttgctggtgg tcataatata ttggtgttta caattaggat cgttattatt
     2041 atcttctgtt attgttatcc cctcttcaga aagaaattca aatagatttg ccctgtcatc
     2101 atcactgaat tttggaatgg tgtattcaaa gttctttgtg tctgaataca aacagttttc
     2161 ttttataatc aaggcgattt catcaaagta agtgttattt tgcccagacg ctcttccgat
     2221 agtggtattt ccgcctgatg gcattagttt tattaagaag tctattcctt tatatgtgcc
     2281 agatatgtga gtttctcttt cgttttttac attagaggaa tagtttgtga cgccattctg
     2341 cgtcagagca gactcaatct tgtcaatatt gatatttagt gctttaaacg ggttctgtgc
     2401 cattgggtca atccgttgtt ttttttgaat atgtacagat cttgtttttt tgtcaacgga
     2461 atagctgttc gttgacttga tagaccgatt gattcatcat ctcataaata aagaaaaacc
     2521 accgctacca acggtggttt tctcaaggtt cgctgagcta ccaactcttt gaaccaaggt
     2581 aactggcttg gaggagcgca gtcaccaaaa tctgttcttt cagtttagcc ttaacaggtg
     2641 cataacttca agacaaactc ctctaaatca gttaccaatg gctgctgcca gtggcgataa
     2701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2821 ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tggcttgagc gtcgattttt
     3001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgcggcgttg gcttcttccg
     3061 gtgctttgct ttttgctcac atgttctttc cggctttatc ccctgattct gtggataacc
     3121 gtattaccgc ttttgagtga gctgacaccg ctcgccgcag tcgaacgacc gagcgtagcg
     3181 agtcagtgag cgaggaagcg gaagagcgcc ttatgtgaca ttttctcctt acgctctgtt
     3241 gtgccgttcg gcatcctgtc ctgagagtta tctctctgtg ctattttcta cttcaaagcg
     3301 tgtctggatg ctgttctgga gttcttgggg tctgacgctc agtggaacga aaactcacgt
     3361 taagcaacgt tttctgcctc tgacgcctct tttaatggtc tcagatgtcc tttggtcacc
     3421 agttctgcca gcgtgaagga ataatggccg agcatattga tatgtccgtg gcaaagcggg
     3481 gagaggcgtg cgatatcttc atcattcagt gtttcaccct gcgcccggag atgatccagg
     3541 gctgcctgca tataaatagt gttccataac acgacggcgt tagtgaccag ccccagtgtg
     3601 cccagttgat cttcctgacc gtcggtatat cgttttctta tctcaccttt ttgaccgtga
     3661 cagatggctc tggcaacggc atggcgactt tctccccgat taagctgggt cagaatgcgc
     3721 cggcggtaat cttcatcatc aatataatta agcagataca gcgttttgtt gatgcgcccc
     3781 acttcaatga ttgcctgagt cagtccggaa ggacgttcac ttttcagcaa tgaacggacc
     3841 agcactgaaa cctgtacttt gcccagcttc agggagccag cggtccggat catttcgtcc
     3901 cactgaagga ctatttttcg gggatctgat tgccctctgg caatatcatt cagcacgcca
     3961 tagtcggcat catggtccat tcgccagaaa accgaagcac cggcatcagc caggcgtgga
     4021 gaaaactggt atcccagcag ccagaaaagg ccaaagacaa gttcgctggc acctgctgta
     4081 tcggtcataa tttcggttgg attcagcccg gtctcctgtt ccagaagacc ttccagcaca
     4141 aagatagagt ccctcagcgt ccccggtata acgatgccat gaaagccgga atactgatcg
     4201 gacacaaagt tgtaccaggt gatccctctg ttattaccaa agtatttgcg gttcggtccg
     4261 gcattgattg ttctgactgg cgtaacaaag cgcattccat ctgcagatgc cacttctcct
     4321 ccaccccata tctgtgccag tggcagcgtt gcctgaaaat caaccagtct ggcattagcg
     4381 ctggtgatag tttcagcccg cagatagttc gcttttgtcc agttcagccg gtgtcgggtc
     4441 agtgcaggaa catttgatct gatcagtggt tccagaccga tattgcaggc ttcagccatc
     4501 agcacggcgc tgatgctgac gggcagatca tcaactctgg cactggcttc actagcatgg
     4561 aaaaactcat cagcaaatcc ggtatgggcg ttaatttcga gcagcaactc cgttaaatcc
     4621 accggaggga gtagatcact gatcattttg ctcagtcgtt tcagactgtc cggctcatca
     4681 agactggcga ggggagaaat tgtcaaccgg ggcttcgggc cagaaacatc gagttcgaca
     4741 gcctcatttt cgcaaagacg tgcagcaacc tgtctgtaac gactatcaag ctgatgaccc
     4801 agagatttta ttgcttcctg cgggtctgtc gggtgcccca aagaacgata aaccttaatc
     4861 cggtttgcct gccagtcagc accctgtagt aatcttgcac gaggatctcc ccaccggtta
     4921 ctgccggtaa cgtagacatc cctccgcctc agactatcct gcagtttact gagaaagcag
     4981 agcgtgtatc ccctgcgggt gatatgtttt tccttgttaa tcaccagccg tttccatgac
     5041 cgactgataa tttccgttgg tgcgtcgtca aaaaactgcc gccgtgagct gaactcccgg
     5101 ctgaggtagt cacaggcatt cagagtggta accccggcag gtgcggatga aaatttaacg
     5161 gtattcagca gatggggcag gaaacgacga acgcgcccgt actgctccac catttcttca
     5221 tgaaaattat cgtctgaggg ccgggcaatt tcacggacaa gcgtgatgat ttcagccagc
     5281 ttttgccttg ggatgtagct gaacacctca gcacgaatcg attcgtccgg tgtttcttct
     5341 ttcagcaggt acgaacatgc gctggcgagc gccaatgcag atttatccag atccttcagc
     5401 gagcggagcc gttttttctg cccaatcttt ctggcgtcac ggatgataac ggccagcatg
     5461 gcgtccagaa cgtccaatgc atcatccagc gccagcgttt cccatgcaag gacaaaggca
     5521 accagaaccg ccatcctttt ctgcggtgac atcctggcaa tattgaacac cgaagtcata
     5581 ccagcataac gtgcgagatt tttcaggcgc acagccggga gtgtactcag gttttcagca
     5641 tgcaggccaa aatcgttcag agttttccag cgttcaattg cttcattaaa cgccggacca
     5701 ctgatggtca cagggccctt tttcagtgat tccagtaaag acaggcggct gcaatcagtt
     5761 ggccccagca gcatctccag ctgtgaacgc tgttcggctg acggtatcag tgccagtttg
     5821 ttccacaggc gcaacgtcgc cttttccctt acctctgaaa tcaaccgggt cagcgtagtg
     5881 gctccgggga gaataatacg atgttgcata agccaccctg tcgccagatc gaaaagcagg
     5941 ccaggacgtt cgttgcttat ccagctccgg gtatataaaa gacgggtaag gcgaaatgtc
     6001 cagggccagg caaattcacg atactgatag tgctgacgta tcagcgctgc atgctcacgg
     6061 cgggtatttt ccctctgacc gtattctgca agaacggtga tatcacgaat cccgagctgt
     6121 ctggcggtaa aatgccggac gccggaagga atatgattca tatcggtgag gaaggtgccc
     6181 agaaaacgga cacatccaat ttgcagggca atgcccagac ggttgtgatc acctctgctt
     6241 tttccgataa attccttgtc tgcttcatca aggtgaaaat atcgtgccag ctgaagctca
     6301 tccggttcac cggtgaatct gccatagctt tcagtctgct cagtggtcag aaagtcaacg
     6361 ggcatatcgg cctccctgcc tgacgggcat ttagtaacat ttttccaacc gtacgaaatg
     6421 ttataaatta tcggacatcg taaaactgtt acattaatat gtctattaaa tcgtaaattt
     6481 gtaataatag acatgagttg tccgatattc gatttaaggt acatttttat gcgacttttt
     6541 ggttacgctc gggtctcaac cagtcagcag tctcttgatc ttcaggtcag agcactcaaa
     6601 gacgcaggtg tgaaagcaaa ccgtatattt accgataagg catccggcag ttcaacagac
     6661 cgggaagggc tggatttgct gaggatgaag gtggaggaag gtgatgtcat tctggttaag
     6721 aagctcgacc gtcttggccg cgacactgcc gatatgatcc aactgataaa ggaatttgac
     6781 gctcagggcg tggcagtccg gttcattgat gacgggatca gtaccgacgg tgatatgggg
     6841 caaatggtgg tcaccatcct gtcggctgtg gcacaggctg aacgccggag gatcctagaa
     6901 cgcacgaatg agggccgaca ggaagcaaag ctgaaaggaa tcaaatttgg ccgcaggcgt
     6961 accgtggaca ggaacgtcgt gctgacgctt catcagaagg gcactggtgc aacggaaatt
     7021 gctcatcagc tcagtattgc ccgctccacg gtttataaaa ttcttgaaga cgaaagggcc
     7081 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7141 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7201 caaatatgta tccgctcatg agacaataac cctggtaaat gcttcaataa tattgaaaaa
     7261 ggaagagtat gagtattcaa cattttcgtg tcgcccttat tccctttttt gcggcatttt
     7321 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7381 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7441 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggtgcgg
     7501 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7561 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7621 gagaattatg cagtgctgcc ataaccatga gtgataacac tgctgccaac ttacttctga
     7681 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7741 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7801 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7861 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7921 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7981 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8041 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8101 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gacc
//
