LOCUS       NZ_CP107167            15472 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Escherichia coli strain R9 plasmid pR9-658-3, complete sequence.
ACCESSION   NZ_CP107167
VERSION     NZ_CP107167.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31158869
            Assembly: GCF_030707935.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15472)
  AUTHORS   Jaen-Luchoro,D., Kahnamouei,A., Yazdanshenas,S., Lindblom,A.,
            Samuelsson,E., Ahren,C. and Karami,N.
  TITLE     Comparative Genomic Analysis of ST131 Subclade C2 of ESBL-Producing
            E. coli Isolates from Patients with Recurrent and Sporadic Urinary
            Tract Infections
  JOURNAL   Microorganisms 11 (7), 1622 (2023)
   PUBMED   37512795
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15472)
  AUTHORS   Jaen-Luchoro,D., Kahnamouei,A., Yazdanshenas,S., Lindblom,A.,
            Samuelsson,E., Ahren,C. and Karami,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2022) Infectious Diseases, University of
            Gothenburg - CCUG, Guldhedsgatan 10a, Box 7193, Gothenburg, Vastra
            Gotaland 40234, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 338x
            Sequencing Technology :: Illumina NovaSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/11/2023 07:51:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,176
            CDSs (total)                      :: 5,061
            Genes (coding)                    :: 4,871
            CDSs (with protein)               :: 4,871
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 69 of 190
            Pseudo Genes (incomplete)         :: 138 of 190
            Pseudo Genes (internal stop)      :: 38 of 190
            Pseudo Genes (multiple problems)  :: 49 of 190
            Pseudo Genes (short protein)      :: 3 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107167.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="R9"
                     /isolation_source="human"
                     /db_xref="taxon:562"
                     /plasmid="pR9-658-3"
                     /geo_loc_name="Sweden: Gothenburg"
                     /collection_date="2018"
     gene            complement(join(14360..15472,1..2700))
                     /locus_tag="OGM38_RS25785"
                     /old_locus_tag="OGM38_25755"
     CDS             complement(join(14360..15472,1..2700))
                     /locus_tag="OGM38_RS25785"
                     /old_locus_tag="OGM38_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528154.1"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="WP_000705882.1"
                     /translation="MKITVLNQRYKKHFGYPHILMGDVKVTVSAMIACAVLLHPNKLFA
                     SVAGTSLPYQTYRDFAENKGEFRPGAENVPLYDKNGNPVTTLNKAPMIDFSSNDSTGVA
                     TLVSPQYVVSVKHNVGYQYVKFGYADDSSYALVDRNNHWRDFHTPRLNKIVTEVTPLDI
                     TNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGAYSYKTGGIVNTPAISDW
                     SFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLNSYAGVNGNTNWYTIIPAG
                     DVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGSTSWEMHGNLGATWPASLNS
                     GKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQTWKGGGIIVNGEHLVDWQIN
                     GVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQRADDNGLSQAFSSVSIVSGRP
                     TLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLNGADDGAILTNSGSMANVNLDFN
                     SPDTTATIANIWHGHFTGNLNINNEVTAGTQNDFAIDGGVNAQGSITQQNGRLFMQGHP
                     VVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWENRQFSMAELNLQNAEFNLARNASL
                     NTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTLGKSKATAEDDQSRFNGHVQLQQGSA
                     LTINEHFAGGIDSADSATTITSTDTTLNQLSRFTQSSLSLGEGAKLTATAGLLSDGTVS
                     SNAGASLSLLSDQPGTMYSAQSWELSGQDTSLNVGAGGIITGDINANDAASISFGTTDI
                     NQSTNYYGNINAPLASVTMKDTAWQVNKQSVAKSLTLNGSTLSFNRFGQGGLTSDTLEA
                     TNSSFIINADGKAADTVTVNQALTGGNNTLVVIPTTNSVKQGGDPVSLVTAPKNTQSNI
                     FTLNPVSINAGFHSFTPQLDVLETDVNKQWRLEGFYIQPDKAALRTGKSFMDLGYKNFI
                     TEINNLNDRMGDLRHTHGETGAWARLNSGSGSATDGFTGSYTHLQIGADRKHIIEGGEL
                     FTGVTATFTSSNNRGTGWSGRTKSTGIGVYASAMFDSGLYVDTIGKYVRHDNHYSSSAL
                     GMPEQDYGSHSWYLGAEAGWRFSLPDETYIQPQTELIYGTVSENQFAWQFNGGEIYMQR
                     KQMQPLIGRTGIEFGKTFRGKDWEMTALTGINYQYDLFKPTVTAFKDLAGDTYINNGKD
                     SRVVFNVGVNTKIKENTRISLNVERSEFGSYNIDKLINANIRYTF"
                     /name="S6 family peptidase"
     gene            3101..3625
                     /locus_tag="OGM38_RS25790"
                     /old_locus_tag="OGM38_25760"
     CDS             3101..3625
                     /locus_tag="OGM38_RS25790"
                     /old_locus_tag="OGM38_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000491818.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVTL
                     PNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYVYP
                     ALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISNNYNKNHTGAW
                     SAQYTLTATYN"
                     /name="hypothetical protein"
     gene            3690..4382
                     /locus_tag="OGM38_RS25795"
                     /old_locus_tag="OGM38_25765"
     CDS             3690..4382
                     /locus_tag="OGM38_RS25795"
                     /old_locus_tag="OGM38_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760483.1"
                     /GO_process="GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="WP_000760483.1"
                     /translation="MKKYNITILSFIFFSHISLAETGGIYLDTTRVIFDSNDLSTKVKF
                     NNNTDKNWLLRAWVSDYNSKNKNDNFIITPPLYKISANESFQFKIDKLKENFPNDRESI
                     FHINVMAIPPITNDEKDSNNAVQFAINTRIKLIYRPHKINNKNQVSDAYKNLKITSSKE
                     TLTVSNPSPYYITMNDVKINGKNITSIKDFMVSPFSTLIIPEKNAKTISYSTINDYGGE
                     TPIQNIKL"
                     /name="molecular chaperone"
     gene            4415..6952
                     /locus_tag="OGM38_RS25800"
                     /old_locus_tag="OGM38_25770"
     CDS             4415..6952
                     /locus_tag="OGM38_RS25800"
                     /old_locus_tag="OGM38_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296664.1"
                     /GO_function="GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="WP_001296664.1"
                     /translation="MKGSLKKSLLLLRITIICPVFSCAAVSPSPDNSHRFSFNPDFLQL
                     SDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIKIDFFAQDNKLNARLTPQQ
                     LERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQKLILNIPQSYLKPQDWLSTP
                     PHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSSLNLGGWRLRHNGNWSTNSYN
                     KESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDSFPFEGAQFSSDDGMIAPELSQ
                     YSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELRDFNQIFTGDLEVEIREADGTIR
                     HFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNSVSEPQFVQSSAAIGLPYEYTFYG
                     GGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQFSQNYKSFGKQQGQSYRFMYSRGF
                     GETNTTLNITGYRYATRGYYDFDELQQIQSGFVDEKNINSYHQRSRISTTISQDLQDWG
                     QLYLSASKDQYWDTADGYNLSASYSLPFRYISAMLSLGYNKSPYYHEADKSLFLSVSVP
                     LNSLLNGNNLFLTTNTITNNGQVQQQVGLNGSSQDGEFNYAVAQGWQNQNRGESGNINL
                     NYRGPYAQMNGGYAWQKESSQWTYGISGGITLHPHGLTLSQPLSLDSASALVQARDAAS
                     VKVLNGSGIYTDWRGYAVVPYLNPYNRNQISLDVNSVKDNVELLNTDVTVIPSRGALVS
                     APFKVNVGNKAIITLIQKNGEPVPFGSVVTLDSENSINSSIVADQGQVYMSGLPEEDTL
                     IAQWGEEASQKCKTNYKLQNKKAKFSELTLQCQ"
                     /name="fimbria/pilus outer membrane usher protein"
     gene            complement(7062..7334)
                     /locus_tag="OGM38_RS25805"
                     /old_locus_tag="OGM38_25775"
     CDS             complement(7062..7334)
                     /locus_tag="OGM38_RS25805"
                     /old_locus_tag="OGM38_25775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000405645.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESLK
                     GQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKIREYDDIRS"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(7548..11477)
                     /locus_tag="OGM38_RS25810"
                     /old_locus_tag="OGM38_25780"
     CDS             complement(7548..11477)
                     /locus_tag="OGM38_RS25810"
                     /old_locus_tag="OGM38_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231487.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015474 - autotransporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="WP_001080129.1"
                     /translation="MNRIYSLKYCHLTQGLIAVSELASRVSSKTGRKLLAIFIVSALSY
                     GAAEYACAAQMDTRNFWIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPDFS
                     PVSAKGATTAIGNAYSVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLDKFV
                     VETAGATEHADFNLSAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYNPLLR
                     SASMFQLNWNNMYASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKVFSSKD
                     TWAFFARYDQNTVDILKNTFTQEVNLNGQKMTVNNKNIAINDKITAIELTKSNKNKDLK
                     FHGGGSIELTDNLNSGTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDWSVKGEAN
                     DNLHKTGAGTLNVNVAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLGQADALDKN
                     SDYRGIYFTSRGGTLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQNPSRYVYHGS
                     ITGNTNIEHTGTQKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHAIFREGPRHCY
                     VPGVLCDKDYVTDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHFRFKTLNLENSE
                     FTTARNSVVEGDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQDVREGRSADDGS
                     SSYTGKITLQKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGVFVNSTLSVRDGGH
                     LTAQKGLYSNGRVQIGKKGTLSLSGTPENGADNTWMPVLTYMTEGYDLTGDNATLNISQ
                     QAHVSGDVHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNAAYYGAITGGKGNVSM
                     NNGLWQLTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATGSDFVLRTDLKDADKIS
                     ITEKASGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIFKAGTRVTGFSRVTPTLR
                     VDTTGGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTEVNNLNKRMGELRDTNGDA
                     GAWARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLFTGVTMTYTDSSADSDAFSG
                     KTKSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFAGLGTKHYGTHSWYAGAETGY
                     RYHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMKNKDFSPLIGRTGIELGKTFRG
                     KDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGEKDSRMLFNVGMNAQIKDNMRFG
                     LEFEKSAFGKYNVDNAINANFRYMF"
                     /name="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(12632..13462)
                     /locus_tag="OGM38_RS25815"
                     /old_locus_tag="OGM38_25785"
     CDS             complement(12632..13462)
                     /locus_tag="OGM38_RS25815"
                     /old_locus_tag="OGM38_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158921.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_001088099.1"
                     /translation="MNSQNALSNMRLAVRGDISGNSTLVLKKIRFYNCAIIYLRNAQLL
                     VKTIDGGVINIPPESICYIEKNTVIDVTLNVLGKGMPYDIFHIDNNILSCICKVMEPLL
                     LNPHKIGPMRSRIFNCMADKTDTEIFKMITEANVPNHRLIYNITYLLSKVDDIESLVCS
                     LSVSTDSTFTEKLKLIIESDLSKSWRLVDLANVLHMSEVSIRKKLEKENNNFNNLILDI
                     RMQHAARLITTTEKHINSISDEVGYVSTSYFIRNFKSYFGITPKQFSLKVKRKS"
                     /name="AraC family transcriptional regulator"
ORIGIN
        1 tgtgttattc ccccctgtca atgcctggtt taccgtcacc gtatctgctg ctttaccatc
       61 ggcattgata atgaacgaac tgttagtggc ctccagcgta tctgacgtca gtcccccttg
      121 cccgaaacga ttaaagctca gtgttgaacc attcaaagtg agcgacttag ccaccgactg
      181 cttgtttacc tgccaggcag tgtccttcat tgttacactt gccaacggcg cgttgatatt
      241 tccatagtaa tttgtggatt ggttaatatc agtcgtgcca aagctaatgc tggccgcatc
      301 attggcattg atatctccag tgatgatccc acccgcgcca acatttaagc tcgtgtcttg
      361 cccgctaagc tcccagcttt gggcggaata catcgttcca ggctggtctg atagcaagga
      421 aaggctcgcc ccggcattac tgcttaccgt cccgtcagaa agtaatccgg ctgtagcggt
      481 caattttgcc ccttcaccta atgacaaaga agattgggtg aaacggctga gctgattaag
      541 cgtggtatct gtagaggtta tcgtcgtcgc actatctgca ctatcaatac cgccggcaaa
      601 atgttcatta atagttagtg ccgatccttg ttgcaactgt acatgtccat tgaaacgact
      661 ctgatcatct tcagcggttg cctttgactt acccaatgtt ggtttggttg ccacgccatt
      721 cccgtcattg agatcaatgt aaagatcctc actacctaac gtcacggtag aatgctcggc
      781 attaattctg gtattaaggc tggcgtttct tgcaaggtta aattcagcat tttgtaagtt
      841 aagctccgcc atactgaact gtcgattttc ccaatcgttc tgagtaaatg acactggttg
      901 ggtcagtact gaattgtcac ccagcgcctt tagcttattt gctacgtcct gactgctgac
      961 ggcgtgaaca accggatgtc cttgcataaa tagccgacca ttttgctggg ttatactccc
     1021 ctgagcattc actccaccat caatagcgaa atcattctgt gttcctgcgg taacctcatt
     1081 attgatattt agattaccgg tgaaatggcc gtgccagata ttagcaatcg tggctgttgt
     1141 atctgggctg ttgaaatcca gattaacatt agccatggag ccactgttag tcagtatagc
     1201 tccatcatca gccccgttaa gtttatgaaa agtcaaactg tttccattaa tgtcgagctt
     1261 gccgccatga tagccccatt tgatattatc cggattaatt tgtttgtcgt cgtttagcac
     1321 cagagtagga cgcccactta caatactgac agaactgaac gcctggctca gtccgttatc
     1381 atcagcacgc tgtgccaata taacggttcc gtctcccaca cttaagcttc ctgggtttac
     1441 accggtaccg ttaattttta acgtacccgt acccagttta tgcaaactat caccagtaac
     1501 cccattaatc tgccagtcaa caagatgttc accattaaca ataattccgc cgcctttcca
     1561 tgtctgagta tcaacgggct tgacaatata atcatcatca aaagtaagtg tgccagcccc
     1621 ctgattaacg gtattttcta acactacagt accgccgccc tgaaaagtta gatctttacc
     1681 actattaagc gaagcaggcc atgttgcccc caaatttcca tgcatttccc atgatgtgct
     1741 tccctgggtt aatgaaccaa gtcccgtttt ttcgtcatat gaccaatgaa tatccccttc
     1801 accttgtttt gtatttactg gtgcatcggc atcttgtttc atcgtatttt ttacatcacc
     1861 agcaggaatt attgtgtacc agttagtatt gccattgacc cctgcatatg aattgagaac
     1921 ggctaacaaa acccatttat tttggtctgc atcccaggca aaaagaggag agccactgtc
     1981 accgggagtg ccgtaggttg agagtggagt attagtggta ttcgtgacaa aactccagtc
     2041 agatatagcc ggcgtattta ctattccacc tgttttatac gagtaggctc ccattaaata
     2101 ggtgattttt ccgttagtat cttttacata ttgagttccc gtacctactc gatagaacac
     2161 agggaaacgg tcagcatttt gataggttcc cttagctgta cccgcattgg ttatatccag
     2221 aggtgtaacc tcggtgacaa ttttgttgag acggggagtg tggaaatctc tccagtggtt
     2281 atttctgtca acaagggcat aggatgaatc atctgcatag ccaaatttca catattgata
     2341 accaacattg tgtttaacgc tgacgacata ctgtggcgaa accagtgttg caaccccagt
     2401 cgagtcatta gagctgaagt caatcatggg tgccttattc agcgtcgtga cgggattgcc
     2461 atttttatca tatagaggaa cattttctgc gccaggacgg aactctccct tattctcagc
     2521 aaaatcacga tacgtttgat agggaagtga ggttccagcg acggaggcaa acaatttatt
     2581 tgggtgcaat aaaacagcgc aagcaatcat tgctgaaaca gttactttta catcacccat
     2641 cagaatatga ggataaccaa aatgtttttt atatcgctga ttaaggactg taattttcat
     2701 atcacctata tttctttttg aacacatttc attccgacaa ttgaatttct attctatcta
     2761 ttcatatatc ttctgactcg aaagatctta aaactaataa atccaagcat tgctcagagt
     2821 caaccctcac gcatatattt tattgtttat tatcaaaata ttctattttt tagaattgct
     2881 attttgtact taaggaatga caattcagca aaaatcccgt catgtacaaa taagtatcaa
     2941 atggttgtat ttttatgact tgaattaatc tttttttttt gaagaataga aaagaggcat
     3001 tgggatgtgt tgctaatttt tgaaagtttt attctcacat ctctttgccc atcactatac
     3061 acataggaaa aatttaagaa aatcaagatt gaggttgaaa atgttttcta agaataaaat
     3121 caccatcgca gctatttcgg ctctgcttat ttctggtacc gcaatggccc agacaagcag
     3181 tggttctgtc tccgctactg gttcttttaa tgccgcgact tgcagtgtta ccctgcctaa
     3241 tgcaacagca tcttttggca cagtaacgaa tcaagaggta aatgctgcaa aagcgtacca
     3301 gaaaataaaa agtgtaaccg cagatcaggg gattagtgtt accgattgta ccactcctgc
     3361 atcactaacg gtagcaacta ctaatttcca gtctaatggc tatgtttatc ctgctctaaa
     3421 cgaggctacc ggtaatcaac aaaaggattt tgcgctttgg atgaaagtcg gtgataaagg
     3481 tgtatcttct tataatgcac caatcgaact tgctaatggt acatatccaa tcgagttgca
     3541 aatgattaaa atatcaaata actacaataa aaatcatact ggtgcatgga gtgcgcaata
     3601 cactttgact gcaacttata actaatatat ttaacaggca tgattactca tgcctgtata
     3661 ttcagcattc tttaatgtga caatataaaa tgaaaaaata caatattaca atattatctt
     3721 tcatattttt ttctcatatt tcgctagctg agactggtgg tatttatctt gatactacca
     3781 gagttatatt tgatagtaat gacttaagta ctaaagtaaa gttcaataat aacacagata
     3841 aaaactggct attgcgagca tgggtgtctg attataattc taaaaataaa aatgataatt
     3901 tcataataac gccgccgtta tataaaattt cagcaaatga aagctttcaa tttaagattg
     3961 acaaattaaa agaaaatttt cctaacgaca gggagagcat attccatatc aatgttatgg
     4021 ctattcctcc aatcacaaat gacgaaaagg atagtaacaa tgctgttcaa tttgccatca
     4081 acacaaggat aaaattaata tatcgcccac ataagataaa caacaaaaac caagtttctg
     4141 atgcttataa aaacttaaaa ataacatctt caaaagaaac attaactgtc tcaaacccta
     4201 gcccatacta tattaccatg aacgatgtaa agattaatgg gaaaaatatt acatctataa
     4261 aggattttat ggtttcacca ttttcaacat taatcatccc ggaaaaaaat gctaaaacaa
     4321 ttagttattc tacgataaat gattacggcg gagaaacgcc gattcaaaac attaaacttt
     4381 aacatttatt taatcatctt tatcggggga caatttgaaa ggttcattaa aaaaatcctt
     4441 attattgtta aggatcacca tcatatgtcc ggtattcagt tgcgctgccg tatctccctc
     4501 acctgacaat agtcataggt tctcatttaa ccccgatttt ttgcaattat ctgatggtga
     4561 taatgccaaa aatatcgatc tcaattattt tgcaaacgca agcggtgcag ctccagggga
     4621 atatactgtt gatgtagtta tgaatgggga attagttgac cgtcagatta agatagattt
     4681 tttcgcgcag gacaacaagc tgaacgccag actgacgcct caacagttgg aacgttgggg
     4741 aattgataca aataagctct ccgtatcttc cccatcggct taccagcaaa atattgcata
     4801 tattattcag ggcgcaacag agaaattcga tgcgaacaac caaaagctaa tattgaatat
     4861 accacaaagc tacctcaaac ctcaggattg gttaagtact ccgccccatc tctgggatga
     4921 aggtatgcca gcgctaatgg ttaactatct atttaacgga attaagcaaa ataataatgg
     4981 ctacggtagc cgtagtcagt ttttatctct ggacagttct ctgaaccttg gtggatggcg
     5041 ccttcgccat aacggtaact ggtcaacaaa ctcatataat aaagagagcc actggcaacc
     5101 cgttagtgtt tatctacagc atgattattc tttcctgcag ggagggcaat ttactgtagg
     5161 gcaaacctca acggatggtg cgatatttga cagttttcct tttgaaggag cacagttttc
     5221 atctgatgat ggcatgattg ccccagaact cagccaatat agtcctgttg tccgtggcat
     5281 tgcttattct caagcacagg tatccgtcaa gcaaaacgga gttgtcattt atcaaaagaa
     5341 cgtcccacct ggaccgtttg aattacgcga ttttaaccaa atattcaccg gtgacctgga
     5401 ggtggaaatc cgtgaggcag atggcactat tcgccatttt actcaggcga cggccgtttt
     5461 accgattctg caacgtcagg ggcgcttacg ctataacctg gcattcggga aatatcgctc
     5521 aagttctact ttgaataata gtgtgtctga gcctcagttt gttcagtctt cagcagcaat
     5581 cggattacct tatgaatata cattttatgg tggtggtatc aaagcagaca actatgcagc
     5641 agttctgctt ggcctgggta aatactcaga tttttttgga gcgttctccc tggatgtcac
     5701 tcatgctcgc tcacaatttt cgcagaatta taagtctttc ggaaaacagc aaggacagtc
     5761 ttaccgcttt atgtattcgc gtggttttgg tgagacgaat acgacattga atattaccgg
     5821 ctatcgttat gccacacgcg gttactacga ttttgatgag ttacaacaaa ttcagagtgg
     5881 attcgttgat gagaaaaata taaacagtta tcatcaacgt tcgcgtatat caacaacgat
     5941 aagtcaagat cttcaggatt ggggacaact ctacctctca gcaagtaaag atcagtactg
     6001 ggataccgct gatggatata acttatctgc gagttactcg ctgccattcc gctatatctc
     6061 ggcaatgcta tcgctcgggt ataataaaag cccgtattac cacgaggctg ataaatcgct
     6121 gtttttatct gtgtctgtac cgctcaactc attgctgaat ggaaataacc tgttcttaac
     6181 cacgaatacc attacgaata atggtcaggt gcaacagcag gttggattaa acggctcctc
     6241 acaggatggt gagtttaact atgccgtggc gcaaggctgg cagaatcaaa acagaggaga
     6301 gtcaggtaac attaacctta actatcgtgg accttatgca caaatgaacg gcggctatgc
     6361 ctggcaaaaa gaatccagtc aatggacata cggaattagc ggtggtatta cgttgcatcc
     6421 acatggatta accttatccc agccattaag tctggacagt gccagtgctc tggttcaggc
     6481 tcgtgatgcc gccagcgtaa aagtattgaa cgggtcaggt atttatacgg actggcgtgg
     6541 atatgccgtg gtcccatatt tgaatcccta taaccgaaat caaatttcac ttgatgttaa
     6601 tagtgttaag gataatgttg agctgctaaa tacagatgtt actgtcatcc cttctcgagg
     6661 agctttagtt tccgctccat ttaaagtcaa tgtcggtaat aaggcaataa ttaccttgat
     6721 acagaagaat ggtgagcccg taccgtttgg tagtgtggtg acattagatt ctgaaaatag
     6781 cattaacagc agtattgttg ctgatcaagg acaggtgtat atgtcagggt tacctgaaga
     6841 ggatacactt attgcacaat ggggtgagga ggcttctcaa aagtgtaaaa caaattacaa
     6901 attgcaaaat aaaaaagcga aattcagtga attaacttta caatgccaat agtcggtagt
     6961 tcttagatta ataaccctgc atatgcgggg ttattttcat ctaacatatt aacaacatca
     7021 ttccagattt gtattacttc cggcggacac aaatcatctt ttcaactgcg aatatcatca
     7081 tactcccgta ttttatcaaa ttcgtgtttc gcaaatgggc atagtgggat aatttttcgt
     7141 ttttcccgac gcattttttc cacgactttc gcaaccagct gtttaccgat cccttgtcct
     7201 ttcaggcttt catcgacatc ggtatgttcg ataatcgcta aattctctcc ggtcggcaca
     7261 aagacaattt cagcgatttg cttgccctgt tcgtcattaa tgtaaaattt attgtggcct
     7321 tcgcgtattt ccattttacc ctcgcttatt tatgacggta tttcagcgca ccgctcgggc
     7381 acgtacgttt gaggttaaat aacttgccgt taccacgtac gcaattgcca gaatttgtag
     7441 tgatgcgatg ggcaccactt tccagggtag tcagagccat acctttacta gctgcattaa
     7501 cccgggagta acatcactac aggcccctct tcaggggcct ctccttgtca gaacatatac
     7561 cggaaattcg cgtttattgc gttatccacg ttgtatttac caaaggcgga tttctcaaac
     7621 tccagaccaa agcgcatgtt gtcctttatc tgtgcgttca tgccaacatt gaacaacatc
     7681 ctgctgtctt tctccccttt gatccgtttc tctccggatg catcacgaag aactgtctcg
     7741 ccattattca gcaggtcaaa ctgccagctg gttccggcac gggctgtcac actccagtcc
     7801 ttacccctga aggttttgcc cagctcaatc cctgttctgc caatcaacgg gctgaaatcc
     7861 ttgttcttca tactcaggtc catctcaccg tctttccagc ggaatgtttt cccggacact
     7921 gcgccgtaaa ccagttcggc ctgaggctca ataaatgtgt cttccgtcag gtgataacgg
     7981 taacccgttt ccgctccggc ataccaggag tgggttccgt agtgcttcgt ccccagaccg
     8041 gcaaagttac ctgtgtagtc attgtcatgg tgaatatatt taccaatcaa atcaatgtag
     8101 gcaccggagt taaacagtgc tgaagcatac agacctcccc ccacggattt tgtcttcccg
     8161 ctgaacgcat cactgtctgc actgctgtcg gtataggtca tcgtgacacc ggtgaacagg
     8221 tcaacaccgt ccagcgcatg ttttttgtca aaaccgacct gaacgtgggt gtagttatca
     8281 ctgtatccgc catcggctga accggcgccg ttcataatac gggcccaggc accggcatca
     8341 ccgttcgtgt cacgcagctc ccccatacgt ttgttcagat tgtttacttc cgtcataaag
     8401 cttttgtagc cggcattcat gaaactgttc gccttcgctg cagccgcttt atcagcttcc
     8461 gtcctgaaac catccagaat ccacttcgta ctgccgccag tggtgtcaac acgcagcgtc
     8521 ggcgtcacac gactgaagcc tgtcacccgg gtgccggcct taaagatatc ccctgatgtt
     8581 ccggccggtg cactgaccag cgggatattc agggactgtc ccggagacgg gttcttcata
     8641 aagctgacat tcagggtgtt gtctgaaccg ctggctttct ccgtaatact gattttatca
     8701 gcgtccttca gatcagtgcg caggacaaaa tcactgcccg tggcatcaag tgtcttaacc
     8761 gtcagggtac ggaaggcacc gttttcttca gactgtaccc ggctgttacg ggtcgtcaga
     8821 ctgttgatgt cggaatctcc ggtcagctgc cacaggccat tattcatact gacgttcccc
     8881 ttaccgccgg tgatggcacc gtagtacgcc gcgttatatc cgctgaacaa cccggcagcc
     8941 agaacagggg agacagaaga ggaaactgag ccagggtttt cggagccaat acgaatgctg
     9001 gatgaactgg ttgcatgaac atccccggaa acatgcgcct gctggctgat gttcagcgta
     9061 gcgttatcac cggttaaatc atagccttct gtcatgtatg tcagtacggg catccaggta
     9121 ttatcagcgc cattttccgg cgtgccgctc agggaaagcg tacctttctt tccaatctga
     9181 acccggccgt tactgtagag ccctttttgt gccgtcagat gaccgccgtc acggacagaa
     9241 agggtggaat tcacgaaaac accactgttc tgcagcaggg catccggtga ggtgacgttt
     9301 accttactgt catgggcttc aataccgcca atgaaccggt tgttgatgtc cagcgtggag
     9361 cctttttgca gtgtaatttt gcctgtatag ctgctactgc catcatctgc tgagcgtcct
     9421 tcacgaacgt cctggcggaa gccaaaacca ttaccggtga tattgatgcc atcatacata
     9481 tcaatgaaca ccggaacgtc gccccccagt ttcagcgttg aattcgatgc gacaatatca
     9541 ccctcaacaa ctgagttacg tgcagttgtg aattctgagt tttccagatt cagagtctta
     9601 aatcggaaat gccgggtttc ccagtcaggt tggtcaaaag aagccacctg attatttgtt
     9661 ttataggcac tgttattttt cttatttgcc tcactttcca gtctggcaaa atcagtaaca
     9721 taatctttgt cacaaagaac tccggggaca tagcagtgcc gaggcccctc gcggaatatt
     9781 gcatgtgatg tggcatgccc ctgaagtctg agctgggaat tccgcacagt aatgtcattg
     9841 cgtgtattaa tgtttccatc aataatcaga ctgctgtcag cacttttctg tgtcccagtg
     9901 tgttcgatat tcgtatttcc cgtgatacta ccgtgataga catagcggga ggggttttgt
     9961 agggaaaggg tcgctgtttt atcagaagta ttggtgataa tggtaccaac atcagttgcc
    10021 gcgatcttct tgaacgactg gctgaacccg tttaaatcca gagtcccccc acgactagta
    10081 aaataaatac ctctgtaatc actattttta tccagcgcat cggcctgccc cagtttgacc
    10141 gttccacggc cactggcaac atagatagca ttgaaagcct tttctgcatt aagaaaaacg
    10201 gtaccgtcac ctgttttcag gttattcccc tgggccacat tgacattcag tgtcccggcc
    10261 cctgttttgt gcaggttatc gtttgcctcc ccttttaccg accagtcgac aaccgtatct
    10321 tttccgatct caacacccgc ccctttatag gctttatctt tcccaataac cgaataatgt
    10381 tgtccctcat caaaaatcaa tcctccggtt cctgagttca ggttatcggt gagctcaatg
    10441 ctcccgccac catggaattt caaatcttta tttttattac tcttggtcag ttcaatagca
    10501 gttattttat cgttaatggc aatattttta ttattaactg tcattttctg accattgagg
    10561 ttcacctctt gggtaaaagt atttttcagg atatcgacag tgttctgatc atatcgagca
    10621 aaaaaggccc aggtatcctt actggagaat actttacctg tcagtgtccc caaaatgacc
    10681 catttttttt tctgattatc atacaagtaa aatccggaac cgctgtcacc tcctgtcact
    10741 tcattataaa acccaccggt attattactt gcatacatat tgttccagtt taactgaaac
    10801 atggaggcac tacgtaataa tggattgtat gtctgtccca ccccataaga tgtaacaccg
    10861 gttgcaccag ctccaacgcg gaaaccgatt atttgtttct ttccattgaa ttcaatacca
    10921 taacgctcta gtgcttctgc tgctgataag ttgaaatcag catgctctgt tgcacccgct
    10981 gtttcaacaa caaacttatc cagacgctgg accgcaaagt cacctttggt cacacgatca
    11041 acataatgat aatctgactg tccccatgtc tgagttttaa tggcatggtg aatagtgcca
    11101 ttatgacttg ctgtaacact gtaggcatta ccaatagcag ttgttgcacc tttagcggat
    11161 acaggagaaa aatcagggat tgttacttca ggaaaactga attctccccc attttttaat
    11221 ggcgttttta ccccgtatgc tccgggctga aatgcacctt tattctgtgc cagatcaaga
    11281 taatcacgga tccagaaatt tcgggtgtcc atctgtgctg cacaggcata ttcagccgca
    11341 ccataagaca aggcagaaac tataaatata gcgagaagtt ttcgaccggt tttagaggat
    11401 actcgggatg ctaattctga tacagctata agcccttgag taaggtgaca atacttaagt
    11461 gaatatattc tgttcacggg taagactcca taacgtttat aacataatac aaaccaatag
    11521 aactatgttt ttattaataa aaagcttcca attaccttta ttaattgcag tttaaccccc
    11581 ccccacgtct gtaaaaaata catctacaat atagatttta tagcagtgtg tttagcatat
    11641 ttacattgca ttaattaaat tgcattgcgc cgccaaaggt tatttagaat ataataacga
    11701 ctccataccc acattgcata atgaagggta aagctacaaa aataaaatca tgaaataaac
    11761 aaaagcatat gacgtttaag tgtttttata tttattgttt tttattttat atcattatgc
    11821 ggcggaattc ttcatttatt ccgcgcgtta acattattgt atcgtatttt tttatttttt
    11881 tttggaaaac aataatgttt ttaacatgtg gatgttttta tattcgaaga ttaattgagc
    11941 acatgggtaa ataattcgtt attatttgag taattctcta aatatgtaga taatggcgag
    12001 ttaacgtatc acctgacaca cttctctccg aaattaagaa gtatttgact catcaagcca
    12061 ataaatgtgt atagaatgat tatacgggat agttacaagt tcaccgatac ggtgcaggac
    12121 tcaatggcga tgtcggtggg agcgtttgtg tcacgatatg atggacgtcc cttcttaatg
    12181 cagtcagtgc catactttta ctgactgcat taatccgaga gtaacatccc atgaacacga
    12241 tcaggattgt tggtatcgat attgccaggc ctgtttttca ggcctatatc tggattaatg
    12301 atgattctgt tgcataggat agaaacacct catgctccaa attgcaggat atcgttcaac
    12361 gattcgagtc cggaacacta ttcgctatgg aggcatgttc aacttctcat tctgggggat
    12421 gaacgttcag ttctatggaa tatcccctga ctattccctg cacaacagca tcatttttgg
    12481 taaaaaaaat aacattattc ttaatatata gaaaaggaac tcgtcctgga cgggtacaac
    12541 ttattaaaaa tgcatatcat tttcacctca ccggaaaacg attaaaaatc agtaagaatt
    12601 gcttctgcct atattatata tgcaatatat attatgattt ccttttcact ttcaatgaaa
    12661 actgttttgg tgttatacca aaataacttt taaaattacg gataaaatat gatgtactaa
    12721 catacccaac ctcatcagat atactattaa tatgtttttc agtggtagtt atcaatcttg
    12781 ctgcatgttg cattctaata tccaaaatca aattgttaaa attattattt tctttttcaa
    12841 gttttttccg aattgagact tcagacatat gtaaaacatt tgccagatca actaatcgcc
    12901 atgattttga cagatcagat tctattatta actttaattt ttctgtgaaa gtagagtctg
    12961 ttgaaacaga taatgagcaa accaaagatt caatatcgtc aactttagat aacaaataag
    13021 taatgttata aattagtcta tgatttggga catttgcctc tgttatcatt ttaaatattt
    13081 ctgtatctgt cttatccgcc atgcaattaa aaattctact tcgcataggg ccaattttat
    13141 ggggattcag taacaatggt tccatgactt tgcagataca gcttaatata ttattatcta
    13201 tatgaaaaat atcataaggc atgcctttac ctaacacgtt aagtgttaca tcaatgactg
    13261 tatttttctc aatataacat attgattcag gtgggatatt aataaccccc ccatctatcg
    13321 ttttaacaag taattgggca ttgcgaagat aaataatggc acaattgtag aatctaatct
    13381 ttttaagaac caatgtagag ttaccggata tatcccctct cacagcaagc ctcatattac
    13441 ttaaagcatt ttgactattc atacagtcac ccccatgctt tgtaacaaca taatttcatg
    13501 ggatatccac tctgtaaact cggaaataac gcaaagatga atacttcccg gtaaaaagtt
    13561 tcgcatcgac atatcaacta atttcataga ctataaataa ttagtcacta tgatgataaa
    13621 attaattgac tctgataata gttacgtcat gaaaataaaa tcagttgatg ttacagataa
    13681 cacctaaaaa gaattggaag cttccgtgtc gtttcagcac caaatttaat ttttatcatt
    13741 cttattctat aataaatttt acttacactt aaacatgtaa tctttgaaat ttcttttatt
    13801 tttctccgct taatgaaata gaggtaaaga atctctcgtt cggtattatt aagagatcga
    13861 agaatttgtt ctggtggata cgtataatca attgataacc gatttccata gtggatactt
    13921 atattatcag cacatatgca tttaacaggc ccccctgaaa aaagaagact gtataaaaaa
    13981 taattactat tatcgcaaat catataaaca tgattatatt gctgaacggc ctcatataca
    14041 gaggcacggc cactgaactc aaaacattta tactcttcat cctgacttga aaaatataag
    14101 agaccatttt ttaaataaaa atcttttgta accattatta gcgcaacggt catagcacat
    14161 ataaaatatt agaaacctga actgaatcca actataatct aaagtaaaga ataaagccac
    14221 tctaaaaaat agcagcataa gataaaattt tacactccag aatatccaat agataagcat
    14281 atttgactag ctataacaat aaatcaataa gaaaacctga acaatatgtt caggttttct
    14341 tttttatcta ctctcctgct cagaatgtat aacgaatatt agcatttatt aacttatcaa
    14401 tattgtatga accgaactca gaacgctcca cattcagact tatcctagtg ttttctttaa
    14461 ttttagtatt taccccgaca ttgaatacca ctctgctatc tttaccatta ttaatatatg
    14521 tatcacctgc cagatccttg aacgctgtca cagtaggctt aaataaatcg tactgataat
    14581 taatcccagt cagcgctgtc atttcccagt ctttgccgcg gaatgttttg ccaaattcaa
    14641 ttcctgtgcg accaatcaat ggttgcatct gcttacgttg catatagatc tcacccccat
    14701 tgaattgcca ggcaaactga ttctcagata cagtaccgta gatcaactct gtttgcggct
    14761 gtatatatgt ttcgtcaggt aaggaaaaac gccaaccggc ctcagcgccc aaataccagg
    14821 agtgcgagcc ataatcttgt tctggcattc ctaacgcact tgatgagtaa tgattgtcat
    14881 gacgcacata tttcccgata gtatctacat agagtccaga gtcaaacata gctgaagcgt
    14941 acacaccaat acccgttgat ttggttcttc ccgaccaacc agttccccgg ttattactgc
    15001 ttgtaaatgt cgccgttact ccagtaaata actctccgcc ttcaattata tgtttcctgt
    15061 ctgccccaat ttgcaagtgc gtataacttc cggtaaatcc atcagtagct gagcccgaac
    15121 cactgttaag tctagcccat gccccagttt caccatgggt atggcgtaag tcccccatac
    15181 gatcattcag attattaatt tctgtaataa agtttttata accgagatcc ataaaactct
    15241 tgcctgtccg caaagcggct ttatcaggct gtatgtaaaa tccctccaga cgccattgct
    15301 tattaacgtc ggtctccaac acatcaagtt gcggtgtaaa actatggaac cctgcattta
    15361 ttgaaaccgg gttaagtgta aatatattgc tttgtgtatt tttcggggca gtaaccaatg
    15421 acacagggtc accgccctgt tttactgaat ttgtcgtagg aataacaacc ag
//
