LOCUS       NZ_CP107119             6896 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Escherichia coli strain S13 plasmid pS13-S6K44-2, complete sequence.
ACCESSION   NZ_CP107119
VERSION     NZ_CP107119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31158884
            Assembly: GCF_030708155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Jaen-Luchoro,D., Kahnamouei,A., Yazdanshenas,S., Lindblom,A.,
            Samuelsson,E., Ahren,C. and Karami,N.
  TITLE     Comparative Genomic Analysis of ST131 Subclade C2 of ESBL-Producing
            E. coli Isolates from Patients with Recurrent and Sporadic Urinary
            Tract Infections
  JOURNAL   Microorganisms 11 (7), 1622 (2023)
   PUBMED   37512795
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Jaen-Luchoro,D., Kahnamouei,A., Yazdanshenas,S., Lindblom,A.,
            Samuelsson,E., Ahren,C. and Karami,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2022) Infectious Diseases, University of
            Gothenburg - CCUG, Guldhedsgatan 10a, Box 7193, Gothenburg, Vastra
            Gotaland 40234, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219x
            Sequencing Technology :: Illumina NovaSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/11/2023 07:37:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,381
            CDSs (total)                      :: 5,268
            Genes (coding)                    :: 5,033
            CDSs (with protein)               :: 5,033
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 89 of 235
            Pseudo Genes (incomplete)         :: 172 of 235
            Pseudo Genes (internal stop)      :: 39 of 235
            Pseudo Genes (multiple problems)  :: 58 of 235
            Pseudo Genes (short protein)      :: 3 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP107119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S13"
                     /isolation_source="human"
                     /db_xref="taxon:562"
                     /plasmid="pS13-S6K44-2"
                     /geo_loc_name="Sweden: Gothenburg"
                     /collection_date="2018"
     gene            182..1747
                     /locus_tag="OGM53_RS26865"
                     /old_locus_tag="OGM53_26835"
     CDS             182..1747
                     /locus_tag="OGM53_RS26865"
                     /old_locus_tag="OGM53_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_247762115.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSGI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQKAISQANNKRSTAVSRIHDAEDNLKIAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSMLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            2133..2270
                     /locus_tag="OGM53_RS26870"
                     /old_locus_tag="OGM53_26840"
     CDS             2133..2270
                     /locus_tag="OGM53_RS26870"
                     /old_locus_tag="OGM53_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3294..3485)
                     /locus_tag="OGM53_RS26875"
                     /old_locus_tag="OGM53_26845"
     CDS             complement(3294..3485)
                     /locus_tag="OGM53_RS26875"
                     /old_locus_tag="OGM53_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3550..3873
                     /locus_tag="OGM53_RS26880"
                     /old_locus_tag="OGM53_26850"
     CDS             3550..3873
                     /locus_tag="OGM53_RS26880"
                     /old_locus_tag="OGM53_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_106505284.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRLVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4082..>4513
                     /locus_tag="OGM53_RS26885"
                     /old_locus_tag="OGM53_26855"
                     /pseudo=""
     CDS             4082..>4513
                     /locus_tag="OGM53_RS26885"
                     /old_locus_tag="OGM53_26855"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4551..5081
                     /locus_tag="OGM53_RS26890"
                     /old_locus_tag="OGM53_26860"
     CDS             4551..5081
                     /locus_tag="OGM53_RS26890"
                     /old_locus_tag="OGM53_26860"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_143361689.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNALRLVQE"
                     /name="MbeB family mobilization protein"
     gene            5088..5321
                     /locus_tag="OGM53_RS26895"
                     /old_locus_tag="OGM53_26865"
     CDS             5088..5321
                     /locus_tag="OGM53_RS26895"
                     /old_locus_tag="OGM53_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5882..6301)
                     /locus_tag="OGM53_RS26900"
                     /old_locus_tag="OGM53_26870"
     CDS             complement(5882..6301)
                     /locus_tag="OGM53_RS26900"
                     /old_locus_tag="OGM53_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_016247941.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6382..6819)
                     /locus_tag="OGM53_RS26905"
                     /old_locus_tag="OGM53_26875"
     CDS             complement(6382..6819)
                     /locus_tag="OGM53_RS26905"
                     /old_locus_tag="OGM53_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_247762116.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDRRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     Typing          3122..3205
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.07e-36"
     Typing          complement(2554..3111)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3518..3867
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.08e-64"
     Typing          3864..5425
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.697"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgggtggaaa tagcacgtgg tgtgggtggc tgttttttga tcgttttcac aaaaatgaaa
       61 atccacaggg ttgacaggga aaatgcagcg gcgtaatttt aagctgtata taaaaccagt
      121 ggttatatat acagtatatt gcttttaact atttgttttt aatgtcaaag aggattttat
      181 aatggaaaca gctgtagcgt actataaaga tggtgttcct tatgatgata aggggcaggt
      241 aatcattact cttttgaatg gtaatccaga cgggagtggc tctggcggtg gtggtggaac
      301 tggaggtagc aaaagtgaaa gttctgcagc cattcatgcc acagctaaat ggtctactgc
      361 tcaattgaag aaaacgcagg cagaacaggc tgcccgagca aaagctgccg cagaagcaca
      421 ggctaaagca aaagcaaacc gggatgcgct gactcaacat ctgaaggata ttgtgaatga
      481 ggcgcttcgc cataattcca ctcatccgga ggttattgac ctggctcatg ccaataatgc
      541 agcgatgcag gcagaagcag agcggttgcg ccttgcaaaa gcagaagaaa aagctcgtaa
      601 agaagcggaa gctgcggaaa aggcttttca ggaagcagaa caacgacgta aagagataga
      661 gaaggagcag gctgaaacag aacgccaatt gaaactggct gaggatgaag agaaacgcct
      721 ggcagcattg agtgaagagg cccgggctgt ggaggtggca caaaaaaatc ttgctgctgc
      781 acaatctgag ctggcgaaag tggatgaaga gattaatacg ctcaataccc gtttaagctc
      841 cggtattcat gcccgtgatg cagaaacgaa tacgctgtcc ggaaaacgaa atgagttgga
      901 tcaggcatct gctaaatata aagaactgga tgaaagggtt aaactgttat ctcctagggc
      961 gaatgatccg cttcagagtc gtcctttttt tgaggccacc agactacggg cgagagctgg
     1021 tgatgagatg gaggaaaaac aaaagcaggt aacagcatca gaaacgcgtc ttaaccagat
     1081 tagctctgag ataaatggaa tccaaaaggc tatttctcag gctaataata agcgaagtac
     1141 agcagtttca cgtattcatg atgctgaaga taatttgaaa atagcacaga ctaatctcct
     1201 gaactcgcag attaaggatg ctgtggatgc aacagttagc ttttatcaaa cgctatctga
     1261 aaaatatggt gaaaaatatt caaaaatggc acaggaactt gctgataaat ctaaaggtaa
     1321 gaaaatcagc aatgtgaatg aagctctagc tgcttttgaa aaatacaagg atgttttaaa
     1381 taagaaattc agcaaagcag accgtgatgc aattttcaat gcactggagt cggttaagta
     1441 tgaagactgg gctaagcatt tagatcagtt tgctaagtac ttgaagatta cgggacatgt
     1501 ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag gtgactggaa
     1561 gccactattt cttacattag agaagaaagc tgtagatgct ggagttagtt atgttgttgt
     1621 tttacttttt agtatgcttg ctggaactac attaggtatc tgggggattg ctattgttac
     1681 aggcattcta tgtgctttta ttgataagaa taaacttaat actataaatg aggtgttggg
     1741 tatttaatta ttctttattg atataaacag tcctagcaat ccaaatggga ttgctaggac
     1801 caaacaaagt agattgtata gcataaatag gtttaatttt gccacggggg cattatttag
     1861 gttttttctt tttctgaaaa aatctttctt tatgaactta aaaaatatgt gttcaatagc
     1921 atattttgaa tatgggcata aaatagtgct tatcactatt gcgtatagca tcttatctga
     1981 cgctaggaaa taataccctt cgttgttttt tgttataaga tatatatata taagtgcgca
     2041 gtataggcca aacaaaatat tttttatgta gtatcttaaa ctcataaatt aaacctcgtt
     2101 atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt gtgggaatat
     2161 tcgctataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt cagggaggga
     2221 ctgtggctcc atcatcttct tctaaattga cggggatctc tgttcagtag aaaagatcaa
     2281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
     2341 gcctggggag gcggtttgat cgaaggttaa gtcagttggg gaactgctta accgaggtaa
     2401 ctggcttcag cagagcgcag ataccaaata ctgtccttcc agtgtagccg tagtcgggcc
     2461 actacttcaa gaactctgtt gcaccatttg cgccatcatc gctctgctaa ccaagttacc
     2521 aacggctgct gccagtggcg ctaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2641 gcgaacgacc tacaccgagc cgagatacca acagcgtgag ctatgagaaa gcgccacgct
     2701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2821 cctctgactt gagcgtcggt ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2881 cgccagcggc gtggccttct ctgggttctt gttttttgct cacatgtttc ttttggtgtt
     2941 atcccctgat tctgtggata accgcattac cgtctttgag tgagctgata ccgctcgccg
     3001 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gtcggaagtt
     3061 gcattttctc cttacacatc tgtgcggcat ttcacacccg gcatggcgca cttttcatac
     3121 aatccgcact gatgccgcat ggttaagcca gtatacactc cgctatcgct acgtgactgg
     3181 gtcatggctg cgccccgaca cccgcaaaaa cccgctgacg cgccctgacg ggcttgtctg
     3241 ctcccggcat ccgctcacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3301 ttttcaccgt catccccgaa gcgagcgagg caactgcggt aaagctcatc ggcgtggtcg
     3361 tgaagagatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag cttctccagc
     3421 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3481 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3541 cgcgagagga tgctcacgat acgggttact gatgatgaac atgcacggtt actggaacgc
     3601 tgtgagggta aacagctggc ggtatggatg cggcgggtct gtctgggtga gccggttgcc
     3661 cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg
     3721 aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc cggtgaccgg
     3781 gttcaggtgg tggtcgcact gatggccatc gagagagagt tgcgcagctt acggctggtc
     3841 gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa aaggcggtgg
     3901 cagtggtccg gttgattacc tgttgggaag ggagcgtaac cgggaaggtg caacggtgct
     3961 tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata
     4021 cacgtcaggt gttctgtcgt tcgcggagaa ggagctgccg ccgggaggac gtgaaaaggt
     4081 gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct
     4141 gtgggtggag caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     4201 gctacagagc ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc ctagaattga
     4261 tgcctggcag acgctggtta atcaccatta cgggctgcat gacccgaacg ccccggagaa
     4321 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     4381 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggtc gtcaggatgt
     4441 gattcaggcg ctcacggagg cgggacttga agtggtcagg gtgacgcgaa gcagtatcag
     4501 cattgcagac ccgaacggcg ggaggaatat cagactgaaa ggagcattct atgagcaatc
     4561 ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     4621 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aagcaaggct gtgacatcaa
     4681 acgagacgaa aatcaaagat gctatagccc tgttcacagc ttcgacagag gaatcactga
     4741 aaaaacaccg ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc
     4801 tggcagggaa tacgggcatg atgttactgg ggatggtcat tttcctgttt accgtgagtg
     4861 gcgggacgct ctggtatctt ggcgggatga tacaggcgaa cctggaagaa atcaggaagc
     4921 agaacgagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgagggga
     4981 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     5041 aaaacgggaa acagaatgcc ttaaggctgg tacaggagta acagagcatg acggaactgg
     5101 aaagacattt gctgaacgca ttagagcaac tgcaacagga ctatatgcag cggctgaacg
     5161 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     5221 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     5281 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     5341 ggaacgcgaa catgagttat ccctggagcg tgagcgccag ctggaaatac aggaacggac
     5401 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     5461 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     5521 accttgctga aaacacagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     5581 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttctgttttc
     5641 ccgctccggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     5701 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     5761 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     5821 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagggattat ggtaaatcca
     5881 cttactgtct tccctcatag ccatcgagat aaaccgcaga aatcgtctca gccagcagcc
     5941 tggatttttt cggggtaagg tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc
     6001 cccggagtga gtttaaaaat tcctggctgt gaaactgctg aatgcgttgc tgcacatcac
     6061 tccgtgattt tcctgtcgct ttatcctttg ctcccatatc ccagaactgc cggaaaaaag
     6121 gcaccatcat tcgtgttgtg tcggcttttt gggtggaaaa gttgggatca aaaccgtcat
     6181 cagaagcata tacgtaatgc cgggtatgta gttccacgct gccctttggg gtacaggctg
     6241 tgagagctac tgttgctgtt atcatggcga taagacaaag tttgtttccg gtacgtctca
     6301 tgagagtctc cctaccttaa agtatttatt ttcggtgagt tattatcaga agactatttt
     6361 atcatcagtc catgacagcg ttcagcgacc aaacaaccgg gaccagaatc cccgtttctt
     6421 ttcctgctcc agcgcctgcc tgagctgggc tatctcattc tgtagctgtt cccgttctgc
     6481 ttcctggcgc cggcgctccg tatcctgggc ttgtttatcc tcaagcatca gcgtcaggca
     6541 ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc
     6601 tgcctgtccc gcactgtgcc tttctggtgt ctcatcctgc tttaattcgc cgtatgcccg
     6661 gatcagttca ctggtttcaa actcccgtcg tctgtcctgg ctggtacggt aactgacaag
     6721 gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt tcagtgcgtc
     6781 ctggagtgtg tgccatgcca tgaagtgaca gtgtcccatt ggtgtctcat atcatggttt
     6841 cagtaaaaca tactgaggcc ggagaacggt aatgtttgtg ctggtttttg tggcat
//
