LOCUS       NZ_CP106990            10967 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Acinetobacter baumannii strain NY13623 plasmid pNY13623-2, complete
            sequence.
ACCESSION   NZ_CP106990
VERSION     NZ_CP106990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31100588
            Assembly: GCF_025722955.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of strain NY13623
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2022) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            No.20,Dongdajie,fengtai, Beijing, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 03:52:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,986
            CDSs (total)                      :: 3,891
            Genes (coding)                    :: 3,797
            CDSs (with protein)               :: 3,797
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 29 of 94
            Pseudo Genes (incomplete)         :: 62 of 94
            Pseudo Genes (internal stop)      :: 11 of 94
            Pseudo Genes (multiple problems)  :: 8 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106990.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="NY13623"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pNY13623-2"
     gene            273..1223
                     /gene="repM"
                     /locus_tag="OC371_RS19875"
                     /old_locus_tag="OC371_19875"
     CDS             273..1223
                     /gene="repM"
                     /locus_tag="OC371_RS19875"
                     /old_locus_tag="OC371_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208778.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1216..1791
                     /locus_tag="OC371_RS19880"
                     /old_locus_tag="OC371_19880"
     CDS             1216..1791
                     /locus_tag="OC371_RS19880"
                     /old_locus_tag="OC371_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096619.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSRQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1811..1954
                     /locus_tag="OC371_RS19885"
                     /old_locus_tag="OC371_19885"
     CDS             1811..1954
                     /locus_tag="OC371_RS19885"
                     /old_locus_tag="OC371_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2143..2661)
                     /locus_tag="OC371_RS19890"
                     /old_locus_tag="OC371_19890"
     CDS             complement(2143..2661)
                     /locus_tag="OC371_RS19890"
                     /old_locus_tag="OC371_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            3034..3321
                     /locus_tag="OC371_RS19895"
                     /old_locus_tag="OC371_19895"
     CDS             3034..3321
                     /locus_tag="OC371_RS19895"
                     /old_locus_tag="OC371_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3308..3622
                     /locus_tag="OC371_RS19900"
                     /old_locus_tag="OC371_19900"
     CDS             3308..3622
                     /locus_tag="OC371_RS19900"
                     /old_locus_tag="OC371_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3750..6161
                     /gene="znuD2"
                     /locus_tag="OC371_RS19905"
                     /old_locus_tag="OC371_19905"
     CDS             3750..6161
                     /gene="znuD2"
                     /locus_tag="OC371_RS19905"
                     /old_locus_tag="OC371_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6466..6927
                     /locus_tag="OC371_RS19910"
                     /old_locus_tag="OC371_19910"
     CDS             6466..6927
                     /locus_tag="OC371_RS19910"
                     /old_locus_tag="OC371_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7363..8331)
                     /locus_tag="OC371_RS19915"
                     /old_locus_tag="OC371_19915"
     CDS             complement(7363..8331)
                     /locus_tag="OC371_RS19915"
                     /old_locus_tag="OC371_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_012308653.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQAQYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLEKFAQLVDYHMAFETVAPHV"
                     /name="IS30 family transposase"
     gene            complement(8433..8765)
                     /locus_tag="OC371_RS19920"
                     /old_locus_tag="OC371_19920"
     CDS             complement(8433..8765)
                     /locus_tag="OC371_RS19920"
                     /old_locus_tag="OC371_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390243.1"
                     /translation="MEDSTLNELIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSMAE"
                     /name="hypothetical protein"
     gene            complement(8783..9049)
                     /locus_tag="OC371_RS19925"
                     /old_locus_tag="OC371_19925"
     CDS             complement(8783..9049)
                     /locus_tag="OC371_RS19925"
                     /old_locus_tag="OC371_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050441450.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERTKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            9259..10695
                     /gene="mobQ"
                     /locus_tag="OC371_RS19930"
                     /old_locus_tag="OC371_19930"
     CDS             9259..10695
                     /gene="mobQ"
                     /locus_tag="OC371_RS19930"
                     /old_locus_tag="OC371_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029315.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLNLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRGTLGKVLKREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKKYQKQHPEKAKQYQTLYQSYKIVKKCVDEIKAEQQMKIRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     Typing          274..1223
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9260..10692
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagtttgagc gaacgaagtg aagcgaaaac caagggcaat ttttcactcc ttgggctttt
       61 aattatttta aagtttttaa atgcttttag atatgctgaa agcctttgtt cacaatgctt
      121 ttaaggttta tatgacaccg tttaccttgc aatatgacac cgtttacctt gcaatatgac
      181 accgtttacc ttgcaatatg acaccgttta ccttgcttat agcactaaaa ttaattattg
      241 tgtcatagca taataattaa tactggtgct tcatgagaga attagtggta aaagacaatg
      301 ccttaatcaa tgcaagctat aacttagatt tagtagaaca acgtttaatt ttattggcca
      361 ttgttgaggc aagagaaagc gggaaaggta ttaatgcgaa tgatcccctt gaagtacatg
      421 cagaaggcta tatcaatcaa tttggcgtac atcgcaatac ggcttatcaa gcattaaaag
      481 atgcctgtaa tgatttattt gcaagacaat ttagctatca aaaaataaat gaacgaggga
      541 atattgagaa ctatagatcc cgttgggtta gtgaaattgg atatgtagat aatgaagcag
      601 tggttaaact tatctttgcc ccagccatag ttcccttaat tacacgctta gaagagcatt
      661 tcactaaata cgaattgcag caagttagta atctcagcag tgcttatgct gttcgcttat
      721 atgaattatt aattgcttgg agaagtactg gctctactcc tgttatagag ctaagtgatt
      781 tccgtcaaag aattggcgta ctcgatacag agtacaagcg tatggaacgc tttaaaacta
      841 gtgtacttga gcttgctatt aaacaaatta acgaacatac agatatcact gtgaagtatg
      901 agcaacacaa aagaggtcga tcaatttcag gattctcttt tacttttaaa cagaaaaaga
      961 aggacaaccc accgatagaa agagatccga atacgttaga tctctttaca aagatgactg
     1021 atgcacaacg ccatatgttt gcaaataaac tttctgaact tcctgaaatg ggtcgttatt
     1081 cccagggaac tgaaagctat ccgcaatttg ctgttcgtat tgctgagatg ctgcaagacc
     1141 ctgaccgaat aaaagaacta tacccatacc taaaaaaagt gggatatatg ccatcaaata
     1201 aaaaggacac cgtaaatggc taagttatca ctaagtgaag tatctaaaaa attccatgtg
     1261 gatagatcaa ccatttacag agctgtacgt aatggacgtt tatcacgctc cagtgatgga
     1321 caattcgatc tagcagaggt catccgatgc tttggagaac ctgagcaaac atctcaaaaa
     1381 attgaatcat ctaggcaaga aggtgatgaa tctacaaaaa aacttattgc ccatttagaa
     1441 aatgaagtca aaaaatacca agaacgtgaa gaacggttaa tgcaacaaat tgaccgtatg
     1501 caaacactca ttgagctgaa aagtgttgca cctgccacag cagcaccaca ccaagatgct
     1561 acggcatgcg acaccaagat gccacagcat gcgacaacac aacaagacac tgataacaaa
     1621 aagaataatg aattaaatat tgcagaaaat gtagcagtgc cacagcaaga aactacggca
     1681 taccacaccc aaacgctaca gcatgccacg ttgcaaagtg tggcagtgcc acaacacaaa
     1741 aaacgtggtt tatttggccg tgtgctgaat gccgtctttg ataatgactg agcaataagg
     1801 gtgaaagatc atgccgaaac tgaaagacat tgccctggga attattgtag ctccgctgct
     1861 gatcccgatc atgctgattg catcgtacca ggataaaaag gcgctgaaga aagagctgga
     1921 tgaatgccaa aaagaaaaag accaggcatc ctgattattc aattgagcta atcaaaagtg
     1981 tggaacattg atgaaataat agcgtggaac gtgaaaagtc ataaaaaaag cccgaacagg
     2041 gatgctcggg ctataaacta gaaactacag cgttgaatta taaggataat tatacagcat
     2101 ttcgtataag gtgtattatg ttaattttag gaaatattaa aactaatttt cagacttatc
     2161 ccaccaactt tgagctaaat cagtattttt aggaatacct ataccatcac gatacataac
     2221 ggaaagactt cgcattgctt tcttattacc taactttgca gccttttcac gaagttcaaa
     2281 agctttatta atattaactt ctattccgct atctgcacga gtatatagat cagctaactc
     2341 aaccattgat tcagaatggc cattttctga ggctttctga aaccacttaa aagctttttg
     2401 gaaatgttct aaagaatcgt ttttgtcatc atcttccatt aatctagcat aagtaacata
     2461 tccatcagct aaccaaaaca ttgctgctac atttccattt tctgctgatt taagagccca
     2521 aacttcagct tgattttcat ctgaatcaac accatgacca tatagataaa tctcggctaa
     2581 ttctaactga gcagaagtat ctccattttc tgctttcgct aataaatcat tagaaaaaac
     2641 aggttctgca aatgtttcca ttgaaaggca aactcctaaa gccataaaaa tatttgttaa
     2701 tttattcaat ttttaacctt taaactaaac tgttaggctt gtatattttt atatcatacc
     2761 taaaagaaaa ctctaaaatt agagtatgtc ttatctctat ttaacataat ggcgattata
     2821 cgaagtacac ttgtatatta atataaatat caactactta ataaaaatag aaaaatccca
     2881 aaatgcccaa aaagccgact ttgaaacaag tcggcttttt tatgagcagt tttgatactt
     2941 cttgccaata attttgacta aaaattacac gaatctggtt gagtgataca attttgtgat
     3001 acattattgt atatacaaat ttgaatgttt tttatggaac agtattttga atgggatgag
     3061 gctaagaatc gaaagaatca gaaaaagcat gatatctctt tcgaaactgc aagccttgtt
     3121 tttgaagatc cattacggat ctcaatccaa gacagacata ccaatggtga agaacgttgg
     3181 caaaccattg gacgagttaa aggcgtacta atgttattgg tggctcacac catttttgat
     3241 gaagatgact gtgaaatcat ccgaatcatt agtgcaaggc aagtcactaa agcggagcga
     3301 aacaaatatg agcatggtta gatactcaca caaagagctg aatgagaaat ttggtgagaa
     3361 gcaagatgct gaaattcaac gcttgcttgc taaaggcacg gttcctgatg atcagctaga
     3421 tctatcagat attcctgaaa ttacagactg gagcaatgct gtacgccaaa atcaatttta
     3481 ccgcccagta aagcaacaaa cttcaattcg gttagatgct gatgtacttg cttggttcaa
     3541 agcacaaggc aaaggttatc aaacacgtat gaatgaaatt ttacgagatg ctatgctaaa
     3601 agaattaaaa aatcatcaat aagaatggga gcttaggctc ccatttttga cttcattttt
     3661 aggggggatt accattcaaa aatttaaata gtagatttta taaattttta agtgttatat
     3721 tataatataa cattaaattt ggttgtttta tgctaaataa aagtaaacta tttttagctt
     3781 taattacttt aggggcctcg aaaattttat tggcagctga agggcctgtt actaccttaa
     3841 atacaatagt attaactgca caatctgatg agttagggtc tgagctgtta ggcaaaagtc
     3901 taaatgttag taatcagttt attgatacta gtaaacttaa acagcgttcg acaactctag
     3961 gggatgcctt gggtacggaa ttaggaattc attctaatca atatggaggt ggagcatcta
     4021 ctcctattat tcgtgggcag gaaggaaaac gtattaaagt tttacagaat aatgctgacg
     4081 tgttggatat gtctaatatg tccccagatc acgctgtcac agttgaacca agtttagcta
     4141 agtctattga aattatacgt ggtgcaagta cccttttgta tagttcaaat agtgcggcag
     4201 gggtagtcaa tgtcattgac tataaaattc caacccaaat gcctcaagac ggattagagg
     4261 gtaatactac attacgtttt aatactggca gtaatgagaa gttgaccaca gcaggtgtaa
     4321 cagttggctt aagcccacat gtagctttac gggcagaagg gttatatcgt aatgctggta
     4381 actataaaac tcctcattac cagtctagct cttataattc tttagaagat cttgaaaacc
     4441 aaaacagtat ttataaaaat ttaaaatatt taccagaaag ctgggcagag tcgagagtag
     4501 gtacgcttgg attatcctgg atagacgata atacttattt aggagtttct tatacacatc
     4561 gtcatgatga atatggttta ccagcccata gccatttata tgaaggttgt ggtgcctctg
     4621 caatcggtat cgattcacga attagtggat taaaaaatta tcttttatat tatcctcaat
     4681 tgatggatga gcaagatatt aattacataa atccacgtcc tgattgtcat cagcataatc
     4741 atattcatga aactaatttc tctcataatg ctccttatat tgatttaaat actcgacgtt
     4801 atgatgtgcg tggagaattc actcaaccat ttaccggtat tgacaaaatt cgtacctcat
     4861 tgagttatat agattatttt cacaatgaac tcgagggaga taagattact aactttttta
     4921 aaaatacagg aaaagtaggc cgcatagagt tatcacatca gccattaggg gaattaaccg
     4981 gtatcttggg gctacaatat ttggaacagg ataatagtgc tttatcacca gtccattctc
     5041 aagaaggcca taccacctat ctagataatc agcagctatt aaatcgaaat gtcactaaaa
     5101 actttagtgt atttggccta gaaaaatata actggaacga ctttactttc gaactaggtg
     5161 ctcgtataga aaagcagaaa gttagtatgg attatgatat agaaaaaatt aaagattcaa
     5221 tgaaaccttg gcctaataaa tataacagtc cttatgtaga aaaaaataat aaaatcaggg
     5281 ctcaaaattt aaaatctatt ttagaagctg ttcaacctaa caaagaaaca gctttttctt
     5341 atgcaggtac agtccactgg agatttgctc caaattatat cttatcatta acaggtacac
     5401 atcaagaaag attacctaat gcacaagaaa tgtatactca tggtatgcat ctcgctacta
     5461 attcttttga aataggtaac cgctttctaa gaaaagagaa atccaataat ttagaaatca
     5521 gtctggccta taaggatgat ttacttgatt atcaaatcag tacttactat tatgattttg
     5581 ataactatat atatttacag acactcaatg aagttttagg aactacaaaa gttagagatc
     5641 agcatacact acgtattaat cattatagcc agtctgctgc taatttttat ggattagagg
     5701 gcaacatagg gtatcaattt aattctgttt atcacggctc tctttttgga gattatgtga
     5761 aaggccgttt aacaaattta cctgatgctg taattgcata cgatatctgg aatagagaac
     5821 cgactttagc tccccaaaag gatcgttaca ctcctagatt acctcctgct cgtttgggta
     5881 cacgactaaa agctgatttt gatgaatcgc ttaaaggtga aatagaatat tatcgagtct
     5941 ttaaacaaga taatatttct aaatttgagc aggttacatc aggttataat atgcttaata
     6001 tgactttagc atataaaaat aagctatctc acacagaata tgatttattc tttaaagcga
     6061 ataatttact tgatcagaaa gtctatgccc atgaaacatt tttgccatat attccccaaa
     6121 ttggaagaaa ttttagttta ggcttaaatc ttaatttcta gataagatac tgtaatcttc
     6181 taagaaaatt tcttagaaga tttttcttat aaaatgatta gattaataat taaggtaagc
     6241 gtccgctgaa tcccatacct attttttaat tcgatttagg tttccagcag tgtggcagta
     6301 actcttcaat ctgggtcact ttatgtgtcg gcagcctttt cagcactgaa tagccaccga
     6361 ttttgtagac accttttagc tagataaaaa gcaatttttt tgatctttaa agaatgagga
     6421 tataagctgt ttaaaactta attctaattg gttaataggt gaaaaatgaa attagcagaa
     6481 gctctcttac ttcgaagtga tcaacaaaaa aaaattattt cactaaaaca acgtatcaat
     6541 gcaaatgtat tggtacaaga tggtgatcaa ccatctgaag atccaaatga actactaaaa
     6601 caagtatttt ctttaattca agaatttcaa aaattaagtt atgcaattca tgaaactaat
     6661 gctttaacaa aattaaatga tggccgttct ctacttgctt tattgacttt gcgtgatgaa
     6721 tttgtagaac agcacaaaac cctaactgca gcaataagta atacatctcg tgaatctgat
     6781 cgttatagta cgcgggaaat aaagtggcat aaagttattc cagtttcatc attacaaaaa
     6841 caagctgatg atattagtct taaattacgt gatttaaacg tgttaattca gtcgactaat
     6901 tggaaaattg atttgattga agcctaatag tcctttagat ataatcaagt tgaacatact
     6961 gggcgagtac aagatccctt acttccacaa cataggattg taaataaagt gtagtggctt
     7021 gcaaagcaag tggataagct taaaatttta agtacaaatc aatccatcac ttggcataac
     7081 tcaaatcttt gtgcaaacaa agttacacgt tacttataac taccatgtac tgacagtatg
     7141 ttcacctcaa ttactaaaaa taaagaaatg aacaagcatc agctcaacta catttctatt
     7201 tttaattcag ttcggcattt aacataaaat ctcttatgcg aaattacata ccaactttaa
     7261 ccacattgtg gacaggttgg agtttagaga taagaaaaat ttctagagca tttgctaaat
     7321 aagccctatc acgttcaaag cctatcgtaa tgatgggcga attcaaacat gaggtgcgac
     7381 agtttcaaaa gccatatgat aatcaacaag ctgagcaaat ttctctaatg gtgtaagcca
     7441 atctaacgcc tttctaggac gagtattcag tgacatggca acttgattta aataatgctg
     7501 atctgcctga tttaaatcaa tccctttagg taaatattgc ctaattaaac cattcatatt
     7561 ttcgcatgtg cctttttgcc agggtgaatg tgggtcacag aaatatacat ctatgcctaa
     7621 atcttcttcg agtattttat gttctgacat ctcacgtcca cggtcatagg tcaacgtttt
     7681 acgcagttct gcaggtaaat atttcagagc ttcagttaaa gccttgcgca ctgattctgc
     7741 ctttgcatca ggtaatgttg ccaagataca gagccgtgta tttcgttcaa taagtgttgc
     7801 tatcgaactt ttattgtctt tacctttaat taaatcagct tcccaatgac ccggtatttt
     7861 tctttcttga acttcggctg ggcgctcatg aatagtttta atatcctgta atatagaatc
     7921 ttttttaggt tcaccgttag cttttcgctt tttattttca tgacgtagac aggataataa
     7981 gtcttttttc aactcaccct tgggtaatgc tcgtatcgtt gaataaatcg ttgtatggct
     8041 tacattcatt gtttgatcca aatcaggaaa tgtctttaaa cgctttgcta tttgctgagg
     8101 agaccataaa caacggatcg cttcaacaat aaatttccag aggattgaat cgattttgag
     8161 ttttctgtga ccacgtctac gtctagcaaa agtgttatca gaagcatatt gagcttgata
     8221 aacgtcattg atgctatttc ttttaagctc acgatagatc gtactaggat gtcttttaat
     8281 aagttcagca aattttctgg ctgaaaagcc ttcttttctt gactcaagca ttaatgcagt
     8341 acgatcttca aagttaagat gatggtatga caattttata tactccataa accctttaaa
     8401 ttaattaggt ggtttatgtc gcacttcaag ttttactctg ccatggatcc agcatctttt
     8461 aatcttggat ctatcgcaat atattcagtt ttatcttcat tccatcggca atatttcatt
     8521 accctggcgc ttaagccatt accacgataa cggccagcac ctctagaagt gccaacaccg
     8581 atataagtca ctaaatcccc tttgataaac gcatatacac cttgtttatc gtaattagga
     8641 attttcccaa aattaaaatg tactggtgac cattcaggag gctttcctaa tagatctaca
     8701 ttccagtgat gttcaaagaa taattctgta ttacgtatta actcattaag tgttgaatct
     8761 tccactactc atctctcaaa atttatagat taaatagctt tcggtcacgc tcatccgtta
     8821 aatacttatc catctgagca attagtttag ctctggcttg ttcatcatct tcagtgattt
     8881 ttaacataca ggatccaatt aaaatcttgc gtctggtatc gtcctttcgt gcctgttctt
     8941 tctctttggt acgctctctt gccagtgctg cctgtctttg agcctttaac tgctttagct
     9001 tttcttcctg tgccttgatt ttgctgtcta gtgtttcagt aacgctcatt aatccaatac
     9061 catcattctg ggaaaccatc cctaacataa gcgcacttga atatgcattc aaggtgctat
     9121 atgtatagta atgttttcct cgaagagcgc acttatgcaa actatgtttg caagtccgat
     9181 tggggtctcc ccaatacccc gacaaccgta ttcacggttg tatttcacta cgtgaaaatt
     9241 taaaagcgaa aaatagacat ggcgatttac cacttttcag tcaaaaccgt agcacgatca
     9301 gcagggcgtt ccgccactgc tgcgattgcc tatcgggcag gtgaaaagat ctattgtgaa
     9361 cgtgaaggcc gagaacatga ctacagccga aaaaccggtg tggaatacaa agaaatttat
     9421 ttaccagaag gcgcaccaga gcatttaaag aatcgagaaa aactctggaa cgaagttgaa
     9481 caacgggaga cccgaaaaaa ctcgactgta gccagggaat ttgaaattgc ctttccaagt
     9541 gaactgaacc aggaacaacg tctggccatg ctcgaagaat tatgtgccag catcgtggaa
     9601 agacatcaag tagcagtcga tgcctgtatt catgcaccgc ataccggatc cggcagtgat
     9661 gagcgcaatt atcacgccca tatcctaatg agcacacgta aacttacgcc agaaggtttt
     9721 acagagaaaa cccgggaact cgatcagaaa cacagtggag aaatcgatca ctggcgtgaa
     9781 cactttgccg atatctgcaa tgtgcacctg aatctggccg gatccacagc ccgggtcgac
     9841 caccgcagct acaaagacca ggagaatggc ctggaagcca ccctgcacga aggaccgaaa
     9901 gtcaccgaac tacgtagaag aggcatcgaa accgaaatca gccgaagcaa tgatgaaatc
     9961 aaacagagaa atcaggctca gttgcaatac gacaaaaata tggacgttct aattgctgaa
    10021 aatgagataa aactcagcac actgaaaaca gaacagcaga tccaaatcaa gaatagcgct
    10081 aaaacgccac caattgacga aaaatccctg tttgaggaaa aacagagggg aacccttggc
    10141 aaagtgctaa aacgcgaaat cagcgcaaaa gacgcgaatc tagacctgga ttttatgcag
    10201 cgcaatctca aacaagccga aataaacctg accaagcacc acaaacatca aaacgagttc
    10261 aatcagcagc ttgcccagga gatcgtgaaa agcggactca agcaaagtca tgacaaattg
    10321 caaagcctgg tcgatcaaca caacgaatta acccagaaca aacccttact gtttggaaaa
    10381 aaagcctggg aagcccaacg tgatgagatc taccaggaac acaaaaagtt gaaaggtcag
    10441 catgaacacc agaaaaaaca tggtgtgaag gatttattgg agaatgaaaa gttcaaagaa
    10501 catgcctgga agaaatatca aaaacagcat ccggagaaag ccaagcaata ccagaccctc
    10561 taccagtcct acaagatcgt taaaaaatgt gtggatgaga ttaaagcaga acaacagatg
    10621 aaaatcagac aagaacaaca actcaaagca cagcaacatg cgcctaaaat gaaatctcgt
    10681 ggcatgagtc gttaaacaga gcgagtgtct acgagcgaac tgagaaaatt tgcccatccc
    10741 tttccctgat tacaagctcc ctatccatca atcacctgca taccagagca tcccgcatgg
    10801 gtgcgaactt tccaagtttg attcctaaat cgagcgtagc gagaaaaaaa gctcatgagc
    10861 gaagcgaatt ccgagttgct tttgatcttg cttttgcttt ttctcaaatt cacgaaaata
    10921 tgaaccaaaa attgccccga cgaatcgagc gaaagcgaga ttcaata
//
