LOCUS       NZ_CP106911            10060 bp    DNA     circular CON 02-NOV-2023
DEFINITION  Klebsiella pneumoniae strain BSIKPN-11 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP106911
VERSION     NZ_CP106911.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29985366
            Assembly: GCF_025677745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhang,N.
  TITLE     Clinical characteristics and evolution of 6 KPC-2 and NDM-5
            co-producing Carbapenem-resistant Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhang,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2022) Chinese People's Liberation Army Center for
            Disease Control and Prevention, Chinese People's Liberation Army
            Center for Disease Control and Prevention, No. 20 Yard, East Street,
            Fengtai Road, Beijing 100000, Unknown or unspecified country
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150X
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/02/2023 05:37:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,202
            CDSs (total)                      :: 6,080
            Genes (coding)                    :: 5,894
            CDSs (with protein)               :: 5,894
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 78 of 186
            Pseudo Genes (incomplete)         :: 120 of 186
            Pseudo Genes (internal stop)      :: 24 of 186
            Pseudo Genes (multiple problems)  :: 35 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106911.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BSIKPN-11"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2017-06-09"
     gene            join(9952..10060,1..41)
                     /locus_tag="OE130_RS30880"
                     /old_locus_tag="OE130_30880"
     CDS             join(9952..10060,1..41)
                     /locus_tag="OE130_RS30880"
                     /old_locus_tag="OE130_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1525..3489
                     /locus_tag="OE130_RS30885"
                     /old_locus_tag="OE130_30885"
     CDS             1525..3489
                     /locus_tag="OE130_RS30885"
                     /old_locus_tag="OE130_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3489..4220
                     /gene="mobC"
                     /locus_tag="OE130_RS30890"
                     /old_locus_tag="OE130_30890"
     CDS             3489..4220
                     /gene="mobC"
                     /locus_tag="OE130_RS30890"
                     /old_locus_tag="OE130_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4227..4757
                     /locus_tag="OE130_RS30895"
                     /old_locus_tag="OE130_30895"
     CDS             4227..4757
                     /locus_tag="OE130_RS30895"
                     /old_locus_tag="OE130_30895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4784..4963)
                     /locus_tag="OE130_RS30900"
                     /old_locus_tag="OE130_30900"
     CDS             complement(4784..4963)
                     /locus_tag="OE130_RS30900"
                     /old_locus_tag="OE130_30900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4989..5417)
                     /locus_tag="OE130_RS30905"
                     /old_locus_tag="OE130_30905"
     CDS             complement(4989..5417)
                     /locus_tag="OE130_RS30905"
                     /old_locus_tag="OE130_30905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5581..5895)
                     /locus_tag="OE130_RS30910"
                     /old_locus_tag="OE130_30910"
     CDS             complement(5581..5895)
                     /locus_tag="OE130_RS30910"
                     /old_locus_tag="OE130_30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5883..6161)
                     /locus_tag="OE130_RS30915"
                     /old_locus_tag="OE130_30915"
     CDS             complement(5883..6161)
                     /locus_tag="OE130_RS30915"
                     /old_locus_tag="OE130_30915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6336..6701)
                     /locus_tag="OE130_RS30920"
                     /old_locus_tag="OE130_30920"
     CDS             complement(6336..6701)
                     /locus_tag="OE130_RS30920"
                     /old_locus_tag="OE130_30920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6698..7069)
                     /gene="tolA"
                     /locus_tag="OE130_RS30925"
                     /old_locus_tag="OE130_30925"
     CDS             complement(6698..7069)
                     /gene="tolA"
                     /locus_tag="OE130_RS30925"
                     /old_locus_tag="OE130_30925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7343..7588)
                     /locus_tag="OE130_RS30930"
                     /old_locus_tag="OE130_30930"
     CDS             complement(7343..7588)
                     /locus_tag="OE130_RS30930"
                     /old_locus_tag="OE130_30930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7915..9600
                     /locus_tag="OE130_RS30935"
                     /old_locus_tag="OE130_30935"
     CDS             7915..9600
                     /locus_tag="OE130_RS30935"
                     /old_locus_tag="OE130_30935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9610..9867
                     /locus_tag="OE130_RS30940"
                     /old_locus_tag="OE130_30940"
     CDS             9610..9867
                     /locus_tag="OE130_RS30940"
                     /old_locus_tag="OE130_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             2915..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(145..723)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3490..4217
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
       61 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
      121 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
      181 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
      241 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
      301 tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt accggataag
      361 gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga gcgaacgacc
      421 tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg
      481 agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag
      541 cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga
      601 ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac
      661 tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc cgttttgcct
      721 cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg tgtccgcagc
      781 gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc
      841 ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc ctgattaagt
      901 ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt tttcgctgaa
      961 cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg caagtcatcc
     1021 ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct ggacgccacc
     1081 gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc cacgccagaa
     1141 attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac ggtgctggac
     1201 gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg
     1261 cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc
     1321 tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga
     1381 aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc
     1441 catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg ccggtaaacc
     1501 cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat
     1561 atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg ggagtattac
     1621 agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga actctgggcc
     1681 ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc cgatcgctat
     1741 aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga ttcacccggg
     1801 cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac cggcgctgcc
     1861 ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc
     1921 gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc ggctaacctg
     1981 ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc gcggctgctg
     2041 gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa cgcgaaactg
     2101 aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt ccgccacctg
     2161 catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg
     2221 aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtccctcgt
     2281 gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgcg
     2341 atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc
     2401 gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc
     2461 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag
     2521 tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg
     2581 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag
     2641 ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag
     2701 gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg
     2761 gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc
     2821 tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa
     2881 atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag
     2941 attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac
     3001 tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg
     3061 cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctatcgc
     3121 gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat ccgggtggac
     3181 gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag cggcgacagc
     3241 ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac
     3301 gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg
     3361 gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg
     3421 gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga ttttgcgggg
     3481 ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag gcgcggaacg
     3541 cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc
     3601 tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc ctggacagtc
     3661 tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt caggtcagcg
     3721 tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg
     3781 acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac cggctggccg
     3841 tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg tacctgcacc
     3901 gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac ggcagaacgg
     3961 tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg
     4021 ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc tgccctgacc
     4081 ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc gtgaatccac
     4141 agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc gcgtgcttta
     4201 aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt gaggacgtgg
     4261 aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa gacatggtgg
     4321 tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg
     4381 cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt
     4441 actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga atgaagctgt
     4501 gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg
     4561 atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc agcgagcggc
     4621 acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc
     4681 gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc cggtggtggc
     4741 cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct tccccgagct
     4801 tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg cacatgtcgg
     4861 ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac tgatccttaa
     4921 tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc attcccttcg
     4981 gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt cagttctgtt
     5041 gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg gcggcgttct
     5101 tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag atcggccagt
     5161 tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt
     5221 gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg gatcagttct
     5281 gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt tccctgtttt
     5341 acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg
     5401 agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt gtgtaatttc
     5461 tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg gttttctgac
     5521 gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta
     5581 ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg tgacgatcac
     5641 aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt cgccaaaccc
     5701 gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa tgtctggcaa
     5761 tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg ctgccagccg
     5821 atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat
     5881 cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt acttatccag
     5941 gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc
     6001 tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc
     6061 tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc
     6121 ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc tttgtagtca
     6181 tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa atacggcttt
     6241 tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct cactcatcat
     6301 ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc atcatcattg
     6361 agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag actgaattca
     6421 aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg cctcattgcc
     6481 gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc cactcgtcca
     6541 tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt
     6601 accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg taccggagtg
     6661 aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa tccagagggg
     6721 cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg gcgtgggtaa
     6781 cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg gcgctgttca
     6841 gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca taggcctctg
     6901 gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta ctgattttag
     6961 atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta caggccgtca
     7021 ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc gggttgatga
     7081 cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg ttcatttaca
     7141 taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt tgcgtgcata
     7201 ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt
     7261 gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat
     7321 gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt
     7381 ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca cgttcgtcct
     7441 gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc agcgttttgt
     7501 tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg cgcttttctt
     7561 cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac
     7621 agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct ataccatctg
     7681 aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga aattttcggg
     7741 aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga agatgagaac
     7801 taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat actgtgtata
     7861 tgtacagtat gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt
     7921 ggtggagacg gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt
     7981 gggaatgtga acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca
     8041 aacagcgggc cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat
     8101 ccgggggagt ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat
     8161 catagcggta gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct
     8221 gccctggcta ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg
     8281 gccgccgttg ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg
     8341 attgcgatct acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca
     8401 aaaattatga cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca
     8461 ctggaccagg cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg
     8521 cagcatattg cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg
     8581 acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc
     8641 gtaccgaaag gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt
     8701 gattcacgtc cggctggttt tacagccggt ggtaactccc gtgaggccgt tattcgtttc
     8761 ccgaaagaga ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca
     8821 caggtaaaac agcgtcagga ggaagaaaag cgtcgccagc aggcatggga cgccgctcat
     8881 ccggaagagg ggctgaaaag agactatgat aaagcgaaag ctgagctgga tgccgaagat
     8941 aaaaatattg cgaccttaaa cagccgcatt gcatcgacag agaaggcgct ccccggtgca
     9001 agggctgctg ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat
     9061 tttgtgactt ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat
     9121 cttgataagg atattcagaa tcagaatgag aaattaaaag cggctcaggc atctttaaac
     9181 gcaatgaatg aatccttatc cagagataag gctgcactta ccggggcgat ggagagccgg
     9241 aaacaaaagg agaaaaaagc gaaggatgca gaaaataagt taaatgagga aaagaacaag
     9301 cctcgcaagg ggactaaaga ctacggccat gattatcatc cagtacctaa aacagaagac
     9361 ataaagggac ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt
     9421 ggtaagcggg cccgatggta tggcgataaa aaacgtaaaa tttatgaatg ggattcccag
     9481 cacggtgagc ttgaagggta tcgtgccagt gatggcgaac acctaggcgc atttgatccc
     9541 aaaacgggta agcaggttaa agggccagat ccaaaacgga acattaaaaa atatctttga
     9601 gaggtaaata tggggcttaa attacgtttg acatggtttg ataagaaaac tgaagagttt
     9661 aaaggggaag agtattctaa agactttggt gacgatggtt ctgtcattga aagcttggga
     9721 atgcctttaa aggataatat taataatggt gagtttgatt tgaagaaaga atgggttcct
     9781 ttactacagc cattttttaa aaatcaaata aaacctgatg tggataattg ttatatagct
     9841 tttgattatc gggacggtaa ctggtaacaa aaaattatca aggatgattt gaaatactga
     9901 cttccggtgt tcagggatga atgccggagt ctttaaatac agggaaagta tatgaaaaag
     9961 gtaaagacta tttttctttt tatattgatt gttgctggct ttcttctggc cgcatgtcag
    10021 gcaaattata tccgggatgt tcagggcgga acggtggcac
//
