LOCUS       NZ_CP106897             4995 bp    DNA     circular CON 22-JUN-2024
DEFINITION  Enterobacter hormaechei strain EHX_PAT_001 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP106897
VERSION     NZ_CP106897.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30926396
            Assembly: GCF_030324195.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Hanage,W.P. and Regev-Yochay,G.
  TITLE     Plasmids shared between Sink and patient Enterobacter isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Taylor,B.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2022) Epidemiology, Harvard TH Chan School of
            Public Health, 677 Huntingdon Ave, Boston, MA 02115, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 635x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030324195.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 05:09:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,032
            CDSs (total)                      :: 4,916
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 34 of 94
            Pseudo Genes (incomplete)         :: 64 of 94
            Pseudo Genes (internal stop)      :: 19 of 94
            Pseudo Genes (multiple problems)  :: 22 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="EHX_PAT_001"
                     /isolation_source="Patient"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="Israel"
                     /lat_lon="32.047 N 34.84 E"
                     /collection_date="2018-01-01"
     gene            57..380
                     /locus_tag="N6139_RS25040"
                     /old_locus_tag="N6139_25035"
     CDS             57..380
                     /locus_tag="N6139_RS25040"
                     /old_locus_tag="N6139_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            370..>1023
                     /locus_tag="N6139_RS25045"
                     /old_locus_tag="N6139_25040"
                     /pseudo=""
     CDS             370..>1023
                     /locus_tag="N6139_RS25045"
                     /old_locus_tag="N6139_25040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1058..1549
                     /locus_tag="N6139_RS25050"
                     /old_locus_tag="N6139_25045"
     CDS             1058..1549
                     /locus_tag="N6139_RS25050"
                     /old_locus_tag="N6139_25045"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1556..1771
                     /locus_tag="N6139_RS25055"
                     /old_locus_tag="N6139_25050"
     CDS             1556..1771
                     /locus_tag="N6139_RS25055"
                     /old_locus_tag="N6139_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2258..2485)
                     /locus_tag="N6139_RS25060"
                     /old_locus_tag="N6139_25055"
     CDS             complement(2258..2485)
                     /locus_tag="N6139_RS25060"
                     /old_locus_tag="N6139_25055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053504437.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     gene            complement(2621..2824)
                     /locus_tag="N6139_RS25065"
                     /old_locus_tag="N6139_25060"
     CDS             complement(2621..2824)
                     /locus_tag="N6139_RS25065"
                     /old_locus_tag="N6139_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071686691.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            3266..3598
                     /locus_tag="N6139_RS25070"
                     /old_locus_tag="N6139_25065"
     CDS             3266..3598
                     /locus_tag="N6139_RS25070"
                     /old_locus_tag="N6139_25065"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_045332894.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3614..3922)
                     /locus_tag="N6139_RS25075"
                     /old_locus_tag="N6139_25070"
     CDS             complement(3614..3922)
                     /locus_tag="N6139_RS25075"
                     /old_locus_tag="N6139_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_012477691.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4272..4403)
                     /locus_tag="N6139_RS25080"
                     /old_locus_tag="N6139_25075"
     CDS             complement(4272..4403)
                     /locus_tag="N6139_RS25080"
                     /old_locus_tag="N6139_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058645281.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPEKHDLTPLAAATGKGCTRDN"
                     /name="hypothetical protein"
     Typing          4766..4939
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(4273..4799)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          371..1863
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg agcaagatgc
       61 tcaccatgtg ggtgacggag gacgagcacc ggcggctgct cgagcgctgt gacggcaggc
      121 agctggcggc gtggatgcgg cagatctgcc tggacgaaaa gccggcacgt tccggaaagc
      181 tcccctcgat ctcgccggcg ctgctgcgcc agcttgccgg catgggcaat aacctgaacc
      241 agatagcccg ccgtgttaac gccggcggcg gcaccggcca tgacagggta cagattgtgg
      301 cggcgctgat ggccatcgat gccggccttg agcggctgcg gcacgccgtt cttgagaaag
      361 gaacggacga tgatcgttaa atttcatccg cgcggccgcg gcggtggcgc cgggccggtg
      421 gactatctgc tgggtaaaga tcggcagcgt gaaggcgcca ccgtcctgca ggggaagccg
      481 gaagaagtcc gggagctgat tgacgcgtcg ccttacgtta agaaatacac ctccggcgtt
      541 ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat agccagcttc
      601 gagcgggttc tgatgcccgg actcgataaa gatcagtaca gcattctgtg ggtggagcac
      661 gcggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgagct gctgaccggc
      721 aggcggctcc agccttacta cgaccgcgcc gaccggccgc gcatcgatgc ctggcagact
      781 atcgtgaacg gcagactggg gctgcacgac ccgaacgcgc cggaaaaccg ccgggcgctg
      841 gtcacgccgt ccgggctgcc gaaaacgaag caggaggccg cagaagccat tacccgggga
      901 ctgctgaccc ttgcttcgtc cggggagctg aaaagccgtc aggatgtcac tgacactctt
      961 gaaggtgcag gttttgaggt ggtgcgcacc acgaaaagca gtatcagcat tgccgacccg
     1021 gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt taacgctggc
     1081 gaaggactta gagcagcaat cgaaagcgca gcagcagagt accggcgaga tgctgaaagc
     1141 cgcattcagc gagcacgagc agtctgtcag agcggaactg agcgcaagcg cgaggagaat
     1201 cagcgacgcc atcctcgccc acgagcagag tatgagcgag gcgatggaga aaaaccggcg
     1261 gagcgtactg cggactgccg ggcggacatg gctcaccatc ctgatggtgt cagcgctgct
     1321 gatcggcacg agcgggagca ttctgtggtg gcaggggcag cagataaccg acaattacac
     1381 gcatcttcgt cagcaggaag ataccctggc gaaaatgacc gcccggacgt ggggagtgcg
     1441 ctatcaggag agcagcgacg gccggaggtt tcttatcctg ccgccgggga tgcaaaccga
     1501 agccatcccc tacgacggga cgacatggat ccgcctgaaa caggagtagc ggaagatgac
     1561 agagctggaa aagcagttgc tgagcgcatt agagcagcta cagcaggact actcgcaaag
     1621 gctggacgaa tgggagagcg ccttcgcgga atggcggagc atgtgtgggc ttatgcaacg
     1681 ggagaacgcg atgctgaacg agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag
     1741 cgagcagctg cgccgcttgt ccgggaactg aatgccatcg agctccggca gagggagaat
     1801 gagtatcaga aaacactgga gctggagcgc cagccgcaga aaacctaccg tgggccgaca
     1861 ctttaaacca tccaggcagc tgacaacccg cacgaagagg ggctcttatt tctcccgcta
     1921 aataggttcg tgatcaaaaa ggtcacgact tattcaggag cgggaaatga agctgttaaa
     1981 tcttattgaa tcattgctta actgcctgta tctggcggcg gagctgtggg actggctgga
     2041 gaagcatttg tcggttttta aggcattggc gcggatttta gtgtgacttt aacatccatg
     2101 catctggctg catgactatg caccagtgta atattcagcg gctgcgtttc tggtgttttt
     2161 tcgttatgtc tgtcgccact ttctcgcgtc tggcaccgga aacgcagcca accccgtttc
     2221 agcggtgcgg gcaacggatg ttatggtaaa ttattaacta ccaccagtca gggttatcga
     2281 gccagcatgt ggcacagtaa ccttcatgcc cttgattttc ttctggatcc atttttcctt
     2341 cgcaggaagg gcaaacttcc caatacttgg tgtgttcttc atttctcatg ttagttaaaa
     2401 gctctaaaga gtatccctct ttatgaagct caaactcttc cgcagttaat tcgatgacgg
     2461 ttaattcctt gtaatggtaa cccattcgct tgcctcgctt tatgcagctc aatactaaga
     2521 tactgaaaag cattaaatgt cagtgttttc agtatcttac aatgataatc ctatccggtt
     2581 tatacaaggg atattaccgc tttactaagc agaagatgaa ttactctgtg ttatctggcg
     2641 tgcggaaaat ttgcagccaa tggcggcctg gaacgctggt tggaggggcg tactcattat
     2701 cgcggattgt gcagtagcgg ccatattttc gggttaccgt atgattcgtt gtgttgatat
     2761 cacattcctg gaatgaacca ccattacgac ctactttgct gtgaggacca acataaatag
     2821 ccattctaag ctccttcgtc agatagttga ttaccagtat ctaagtagat tttattaatt
     2881 tctatattta tcaatatcat agtgtgcttt gttggctttc cacattgcgt cgataaaatc
     2941 ctcatatctt gatgtaagga aaaccaacac atgtaactgt taggtcttct gcgcctaagt
     3001 caaattgatt tttttttatt caaacagacg tttgaagcac taataagaat tgatgacata
     3061 taagaattta tatactatgg catcgttggc gagattgcca atgaattgtt ggttcaacga
     3121 gcaataacca tgcgcgcact ttcctaaata tagggcggtg tggttttttt atgctcataa
     3181 atcaatcaat caatcggagt ttatatgcga aacgttttaa caatagttaa gttgctaaaa
     3241 atcaataacc tagatgagga tgtttatgtc aaacgacgaa aatgaataca cgttcaaacc
     3301 tgttaatgcc caggctatca aggcattaaa atcactccca gaatgggttc aggctgcatt
     3361 cgtgcacgca ttggattcgg tgttctacgg ggtggatccg gggatgtcca tcgcacactt
     3421 gacggtcatc ggccccggcg ttgttgagtt gagaatcaat ggtcgcccag cttatcgctg
     3481 tgtataccgc agtaaggcac ccggccaggt ggacttactg catgcgtttg tgaaaacgtg
     3541 ccaaggacca gataagaaaa acatggcaac cacagcttcg cgctacaagt tgctctaatc
     3601 aaattggcga tggttacacc atcgcctttt ttacaaccat agtcaggggc gactctgtgt
     3661 ttaatggagt gaagtccata tccagcttgt accccatctt gaaaaccatt tccaacagcg
     3721 tatcgacgga gaatttgtcc agttttccgt tcagcatatt gcttactctt ggttgactga
     3781 tacctaactt ttctgccgct tctgattgcg accatttgtt atcaatcatt agttttctaa
     3841 ttatgataat gagttttgac ttgatcgacc atgtattgaa ctttactggg tctgtactga
     3901 tcagctcaaa tgaatttttc atagaacagc tccacttcgg attacctgta tcgcaatgat
     3961 aaaagttccg gcaaggaact ccttatatat atatacgggc ggggcattat acaagtaatg
     4021 atattgcttt caagaggaaa aagtgtaaag acattttgat cttttaagta aagcttattg
     4081 tgttgacgta tcaatccagc aaagagtagt ctttgcccat aaacgaaaaa accacccggc
     4141 aaggtggttt ttcgaaggtt cattaaaccg gcaagtttat gaaccgtggt aacagggtgt
     4201 acaagaccgc tgccacctaa ttcgtccttc cagtttagcc gtagttggac tgatacttca
     4261 agaactctgc atcagttatc tcttgtacac cctttaccag tggctgccgc cagtggcgtt
     4321 aagtcgtgct tttccgggtt ggactcaagt cgatagttac cggaacaggc gcagcagtcg
     4381 gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgcctt cccggaaccg
     4441 agtgtcaggc gtggaatgag ataaacgcgg ccataacagc ggagtgacac cggtaaaccg
     4501 aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct ggtatcttta
     4561 tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attttgtgat gcttgtcagg
     4621 ggggcggagc ctatggaaaa acggcttcgc cgcggcctta ttgcttccct gctaagtttc
     4681 ttcctggcat cttccaggac atttccgtcc cgccagtaag ccatttccgc tcgccgcagc
     4741 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aatatatcct gtatcgcata
     4801 ttctgctgac gcgccggtgc cgtatttttt ctccttccac atgaagcact tcactgacat
     4861 ccgcattcgt gccaacatag taagccagta tacactccgc tagcgctacg tgactggttc
     4921 agggctgcgc cccgaaaccc gccaaaagcc tgacgcgcct gcggcttgtc caaccccgcg
     4981 ccgaccggga aaggc
//
