LOCUS       NZ_CP106657             5896 bp    DNA     circular CON 14-OCT-2023
DEFINITION  Klebsiella pneumoniae strain KP5076 plasmid pKP5076-3, complete
            sequence.
ACCESSION   NZ_CP106657
VERSION     NZ_CP106657.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30959810
            Assembly: GCF_025490515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Lang,D., Lai,Y., Li,X. and Zhang,C.
  TITLE     Klebsiella pneumoniae strain KP5076
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Lang,D., Lai,Y., Li,X. and Zhang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP v. 4; FALCON v. 0.3; Canu v. 0.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 589.61x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/14/2023 04:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,477
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,220
            CDSs (with protein)               :: 5,220
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 57 of 132
            Pseudo Genes (incomplete)         :: 79 of 132
            Pseudo Genes (internal stop)      :: 17 of 132
            Pseudo Genes (multiple problems)  :: 20 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106657.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP5076"
                     /isolation_source="secretion"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP5076-3"
                     /geo_loc_name="China: Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2021"
                     /collected_by="Zhuhai People's Hospital"
     gene            683..874
                     /locus_tag="N7990_RS27325"
                     /old_locus_tag="N7990_27325"
     CDS             683..874
                     /locus_tag="N7990_RS27325"
                     /old_locus_tag="N7990_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            874..1296
                     /locus_tag="N7990_RS27330"
                     /old_locus_tag="N7990_27330"
     CDS             874..1296
                     /locus_tag="N7990_RS27330"
                     /old_locus_tag="N7990_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032451468.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGPLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2081..2209)
                     /locus_tag="N7990_RS27335"
                     /old_locus_tag="N7990_27335"
     CDS             complement(2081..2209)
                     /locus_tag="N7990_RS27335"
                     /old_locus_tag="N7990_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256557297.1"
                     /translation="MALKPGPKRIAESTGQPDQRQRDNKKTPGNTDKLKPSKSSGK"
                     /name="hypothetical protein"
     gene            2371..2595
                     /locus_tag="N7990_RS27340"
                     /old_locus_tag="N7990_27340"
     CDS             2371..2595
                     /locus_tag="N7990_RS27340"
                     /old_locus_tag="N7990_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="WP_064169727.1"
                     /translation="MLAIRLSDEIESRLNLLAKQTGRTKTFYAREAILTHLDDLEDYYL
                     SAETVARVRRGDEVVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            2579..2845
                     /locus_tag="N7990_RS27345"
                     /old_locus_tag="N7990_27345"
     CDS             2579..2845
                     /locus_tag="N7990_RS27345"
                     /old_locus_tag="N7990_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_064169725.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRHSGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2952..4373
                     /locus_tag="N7990_RS27350"
                     /old_locus_tag="N7990_27350"
     CDS             2952..4373
                     /locus_tag="N7990_RS27350"
                     /old_locus_tag="N7990_27350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064169724.1"
                     /translation="MKYFSTGFFLKGNVEVKDIINECLSWVSTSPFTSFVPASLDMKRG
                     LDEFDIEVGDERIELINFSDDSIMMSCFRYSKISGAHKWVTDVAVNYYFSEGKTWVQVE
                     SSVVTQQAVHKAPVTKTPLIVLRLLDKFSGGRDDVFKVGIEPVLLDGSEHSLGFACDII
                     NGRHKNRLPVVYVSSKYFFKEHPHNVIPGRLARKLSGLAHVVVEPEGHLFSNKLKHEVN
                     NKNSYGGSVGIYWPGGQSISIHKRGSLSAAEFEKEIFKEIITAMTSLAPLRKNGWSEIV
                     SSKNRKAIQVLKDNGSQSSELASLYEEENESLQDEVKELNQKIASYEARIRVLMERTSM
                     QGGVFLKIGNEYELFEGEVLEIIMSSIRKVLPTNYEGGRYHDVLSSISSANPCSTNLLE
                     RDKKLKQALVGYTKMTPKVRGVLNDVGFSISEEGKHYKITYHGDPRYTYILPKTGSDYR
                     GALNAYSDISKKVFS"
                     /name="hypothetical protein"
     Typing          5060..5236
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.264"
                     /coverage="82.0"
                     /evalue="3.5099999999999995e-46"
     Typing          complement(4601..5110)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 agcgcgggag tacaccctat tgctataggt aagtctgttc aaaaaacagg cttaccgtac
       61 aataattctc tatatccaaa ctgacccctg atacgcagct tcccggcgaa tatgaccttt
      121 ttgatttaat ggcgcaggtt actgaagaga acctgcatgc ccctgtggat tccgggccgc
      181 ctgtcggccg ggaagcgtta tagcttttcg cttgcccgcg tacgtggtaa tacgccggta
      241 ttacatttta tttctttatt ttcatgcgat tacaggtgat tgtaatacgt tgcgtattac
      301 ataaataaac ggagatttca gtttttacct tttaattcat gcagttgcga tgaaaatcgc
      361 agctgcgtta ggtgtatgac catttaaggg ccaaaaaatt atcattctgg ccgggtgatc
      421 tgcggaggtt aaggggcgtc ggtgacaaaa aaacgtcatt aacgacctcg ttcatgctcg
      481 catgaacgga ttatttttcc ggactacagg taaactacag aaagactaca aaagctgatg
      541 acttaatcct gatttttagt gcgatgacta cagataaact acaaaataac tacaggatta
      601 cctcagatcc gttctctgaa ccattcgtac atccgacccc cttaccggtt ttacactgag
      661 cactgatgct ctgaggtccc ttatgacgaa acaacagaaa acagcgctga acatggctaa
      721 attcatccag aatcagtctc tgctcctgct ggagaagctt aacgagcttg atctcgatgc
      781 tgaggccgac ctttgtgaga aactccacga tgatgcggag catcttttcc gtacactgtc
      841 ctcgagactg gatgcattac aggatggtaa ctgatggcca gagtgtctat tagtgaagca
      901 gcacggcttg ttaaggttag tcgccctact atttacaaga tgattaacag tggtaagtta
      961 agttacacct ctgttgtaaa gcacgggaag ggtattaaag ttattgatac atcagagctt
     1021 attagagtat ttggtcctct tgatggtgta atcgatgctg taaaatatga tgtaaaaagt
     1081 gacgcagaat ctacaggtat taacagcgtt ggtttacatg atttacaaca tagaatcgct
     1141 ttacttgaag ctgaaaacga tggtcttaaa ggggctgtta aggctcgaga tgagcatatt
     1201 gattcccttc gtcaggctat gcagcttctg gagcacaagc acgaaccttc ttcaccaccc
     1261 cattccccgt ggtggaaatt ctggaaaaaa tcctgatccc gccatgcttg agttttcctc
     1321 tccggccatg ccggggcgtt cgtgaacctt cccaaaaatc gccagatctc tgaacgctcc
     1381 tcacactaaa aatatccaca ccccacgcaa aattaaattt ttgcagataa ctcattaata
     1441 tcagtcagtt ggttgttatt aaattacgca gtattacctt ctgtaaggtc aatccaagtc
     1501 gtcggtgatt cgtgtacgac ttgccgacga ttcgtcatag agtcatcgac gactcactcc
     1561 acactatcag taacttatga cgacttgact ctagagtcga atcgtcgctt tggcgttttt
     1621 ttgaccgcgg tgactctaga gtcaagtcac gagtcgaatc gccggtgaat cgtgtggtga
     1681 gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacccga ttcctgatcg
     1741 tgctgatacg gggtttccgg ctgtgccggc aacgtctcac ggccacgcaa taaattgcgt
     1801 ccccgttcac cgttttcggg taagaagaac atcggaaaca ggacttaccc cggctgaatg
     1861 gcgtgaattt ccgcgctatg cacttgcgcg catactcatg catgccgtaa aaacagagcc
     1921 tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga
     1981 aacgccctga gagcctctca ggcggtgcac gcaacggatg ttatgtttaa tccgtctgac
     2041 cgacaagtac aaaaaaagcc cctaagggct ttgctaacaa ctactttcct gaagatttac
     2101 ttggttttaa cttgtcagta tttccgggag tttttttatt gtcgcgttgg cgctggtcag
     2161 gctgccctgt tgattctgca attcgttttg gtccaggttt aagagccata atggatatcc
     2221 ttcatcgtgt taagaggaaa ctctataact gcatatatcg acagttgtag gtagtcaaga
     2281 tgaatggaaa gattgttttt tgttctttga tttttaatgt tgtataattt tgttatacat
     2341 gtatatcaat ttggattcag agaggtcttt atgttggcta tcagattgtc tgacgagatt
     2401 gagtcccgtc taaacttgct tgcgaagcaa acaggtagga ctaaaacgtt ctatgcgcgt
     2461 gaagcgatcc tgacgcatct ggatgatctt gaagattatt atctttcagc agaaactgtg
     2521 gcacgtgttc gccgtgggga tgaagttgta cattcgtctg aagacgtgag gaagtcgctt
     2581 ggtctggacg attaactatt ccgaccgagc gctcaagtcg ttacgtaaga tggacaagca
     2641 gaacgcacga cggattgtgg attttatgga tttacgtatt gccgttgccg cagatccacg
     2701 ccactcaggt aagccgctta aaggtgagct tggagaattc tggcgctatc gagttggcga
     2761 ttatcgcatc ctatgcgaga tcagggacga cgagctgatt attctggctg ctaccattgg
     2821 ccaccgacgt gaagtctacg actgaagccc gctttgcggg ttttttttat gtgaggcgtg
     2881 aaaaatatgt cgttaacaaa atggcttttt aattttaaaa aatattttac tctaaatcat
     2941 aaggggtgtt tatgaaatat ttttccacgg gcttcttttt gaaaggaaat gttgaggtaa
     3001 aagatataat caatgagtgt ttgtcatggg tgtcaacatc accatttact tcatttgttc
     3061 ccgctagctt agatatgaaa agagggttgg atgagtttga tattgaggtg ggtgatgaaa
     3121 gaattgaact gataaatttt tcagatgatt cgattatgat gtcttgtttt cgttactcta
     3181 aaatatcagg ggcacataaa tgggtcaccg atgttgctgt taattattat ttttcagaag
     3241 ggaaaacctg ggttcaagtt gaatctagtg ttgttactca gcaagctgtt cataaggcac
     3301 ctgtaactaa aacaccactc attgtattgc gtctgctaga taaattctct ggtgggcgtg
     3361 atgatgtttt taaagtagga attgaacctg ttttactgga tggttcagaa cattctcttg
     3421 gctttgcttg tgatataatt aatggaaggc acaaaaacag gcttcctgtg gtttatgtga
     3481 gctctaaata ttttttcaaa gaacatccgc ataatgtgat acctggcagg ttggctagga
     3541 agctatcagg gttggcccat gtggttgtag aacctgaagg ccacttgttt tctaataaac
     3601 tcaagcatga agttaataat aaaaattctt atggtggaag cgttggtatt tactggccgg
     3661 gagggcaaag tattagtatt cataaaagag gtagtcttag tgctgctgaa tttgagaaag
     3721 agatatttaa agagattata actgctatga cttcactggc ccccttaagg aaaaatgggt
     3781 ggtctgagat agtcagtagt aaaaacagaa aagccattca agttttaaaa gataatggaa
     3841 gtcaaagtag tgaactcgct tctctatatg aagaagagaa tgaatccctg caagatgaag
     3901 taaaggagct aaatcaaaag atagcttctt atgaagcaag gataagagta ttgatggaaa
     3961 gaacatctat gcagggtggg gtttttttga agattggcaa tgagtatgag ttgtttgaag
     4021 gtgaggtatt agaaataata atgtcttcaa ttaggaaggt tcttccgaca aattatgagg
     4081 gaggtagata tcatgatgtt ctatcttcaa tatcttccgc taacccatgc agtacaaacc
     4141 tcttggaaag agataaaaag ttgaagcaag cgctagtagg ctatacaaaa atgacgccaa
     4201 aagtgagagg tgttttgaat gatgtcggtt tttcaataag cgaagagggt aagcactata
     4261 aaataactta ccatggggat ccccggtaca catatatttt acctaaaacg gggagtgact
     4321 atcgaggggc gttaaatgct tactccgaca tatcaaaaaa ggtgttttca taaaccagta
     4381 agacatggtg cttgagttca tctcttgctg gatgtatctt agttttgaaa cgaaaaaacc
     4441 accctggcag gtggtttttc gaaggttagt taatcctggc agattctcta accgtggtaa
     4501 cagtcttgtg cgagacatgt caccaaatct gtccttccag tgtagcctca gtaaggccac
     4561 cacttcaaga actctcgata catctctcgc acattctgtt taccagtggc cgttgccagt
     4621 ggcgttaagt cgtgtctttt cgggttggac tcaagatgat agttaccgga taaggcgcag
     4681 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg
     4741 gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt
     4801 aaaccgaaag gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga
     4861 tctttatagt cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt cgtgatgctt
     4921 gtcagggggg cggagcctat ggaaaaacgg cagcgccgta tccttttttt gcccagcttg
     4981 tcatgccgta tgcctttgct cttatccgtg tgcaggtaac gcccgccgca gccgaacggc
     5041 aggagcggca gcgaccgagt gagcgaggag gcgtcatatc accgtaactc ccattttctt
     5101 ccttacgccc tgtagctgca ttttcccctg tcacatgaag cacttttatt aagtgttact
     5161 ccgccgtaac atattgagcc agtatacact ccgctagcgc ttcgtgactg gttcagggct
     5221 gcgccccgaa acccgcgaag gaccgtcttt ttgttttttt actgctaaac tgtcaatatt
     5281 ttctttcggc tgccgttaac tgaggtttcc ttgtttgatt tttcctctgg tatcctgatg
     5341 aatatgagtg atgctgaacg gcagctggct gtgtcagtga tttctgactg cttacgccag
     5401 tctgggggag actccggcaa aatgatgctg gatgcttact atctggtctt atctgagaca
     5461 gatccccggg tcagggagtg catgttggcg ttccttgaat ctgttcgtat taaatatgtc
     5521 accgtctgag ttttttttct gatttttgaa ccatactgtg tgtcaggcgg ccgtcatgga
     5581 tgatggtcgt tgtgacatgt cctatgtcac ggaagattgt tccaatctaa cttgacttac
     5641 cttccgggct agccgcccgg tttttttccg ttcttctgac tgtactccag agcaggtttt
     5701 gccttttttc tactgcgctt tcggagtagg ggtcagtttg gagatcggaa atttttgtac
     5761 gttaagcgca ttatttaacc aatcttggtt cgtaaggggt tgattttgcg tcagtggcta
     5821 tcgcttttaa gttgtcgctc ttgcagtatc gcatttaggt tatccgatta aggtcaaacc
     5881 tctgaaaatg ccgtat
//
