LOCUS       NZ_CP104911             8731 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Acinetobacter baumannii strain YZM-0406 plasmid punamed1, complete
            sequence.
ACCESSION   NZ_CP104911
VERSION     NZ_CP104911.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30929528
            Assembly: GCF_025449195.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Yang,Y.
  TITLE     Alteration of adeS contributes to tigecycline resistance and
            collateral sensitivity to sulbactam in Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Yang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2022) Department of Clinical Laboratory,
            Affiliated Hangzhou First People's hospital, NO.4 Xueshi Road,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:07:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,706
            Genes (coding)                    :: 3,638
            CDSs (with protein)               :: 3,638
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 19 of 68
            Pseudo Genes (incomplete)         :: 40 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104911.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="YZM-0406"
                     /db_xref="taxon:470"
                     /plasmid="punamed1"
                     /collection_date="2017-03"
     gene            632..886
                     /locus_tag="N7285_RS19030"
     CDS             632..886
                     /locus_tag="N7285_RS19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            1158..1529
                     /locus_tag="N7285_RS00005"
                     /old_locus_tag="N7285_00005"
     CDS             1158..1529
                     /locus_tag="N7285_RS00005"
                     /old_locus_tag="N7285_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            1529..1702
                     /locus_tag="N7285_RS00010"
                     /old_locus_tag="N7285_00010"
     CDS             1529..1702
                     /locus_tag="N7285_RS00010"
                     /old_locus_tag="N7285_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(2025..2486)
                     /locus_tag="N7285_RS00015"
                     /old_locus_tag="N7285_00015"
     CDS             complement(2025..2486)
                     /locus_tag="N7285_RS00015"
                     /old_locus_tag="N7285_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(2791..5202)
                     /gene="znuD2"
                     /locus_tag="N7285_RS00020"
                     /old_locus_tag="N7285_00020"
     CDS             complement(2791..5202)
                     /gene="znuD2"
                     /locus_tag="N7285_RS00020"
                     /old_locus_tag="N7285_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(5330..5644)
                     /locus_tag="N7285_RS00025"
                     /old_locus_tag="N7285_00025"
     CDS             complement(5330..5644)
                     /locus_tag="N7285_RS00025"
                     /old_locus_tag="N7285_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(5631..5918)
                     /locus_tag="N7285_RS00030"
                     /old_locus_tag="N7285_00030"
     CDS             complement(5631..5918)
                     /locus_tag="N7285_RS00030"
                     /old_locus_tag="N7285_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            6291..6809
                     /locus_tag="N7285_RS00035"
                     /old_locus_tag="N7285_00035"
     CDS             6291..6809
                     /locus_tag="N7285_RS00035"
                     /old_locus_tag="N7285_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            complement(6998..7141)
                     /locus_tag="N7285_RS00040"
                     /old_locus_tag="N7285_00040"
     CDS             complement(6998..7141)
                     /locus_tag="N7285_RS00040"
                     /old_locus_tag="N7285_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(7161..7736)
                     /locus_tag="N7285_RS00045"
                     /old_locus_tag="N7285_00045"
     CDS             complement(7161..7736)
                     /locus_tag="N7285_RS00045"
                     /old_locus_tag="N7285_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(7729..8679)
                     /gene="repM"
                     /locus_tag="N7285_RS00050"
                     /old_locus_tag="N7285_00050"
     CDS             complement(7729..8679)
                     /gene="repM"
                     /locus_tag="N7285_RS00050"
                     /old_locus_tag="N7285_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     Typing          complement(7730..8679)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaaggta aacgtggaca tattgcaagg taaacgtgga catattgcaa ggtaaacgtg
       61 gacatattgc aaggtaaacg tggacatata agagctaaaa ccctttattc ataaggcttt
      121 caagccattt aaaagtattt aaaaacttta aaataattaa aagcccaggg aatgaaaaat
      181 tgcccttggt tttcgcttcg ctcaaactct attgaacttc gctttcgctc agttcgttgg
      241 ggcaattttt tggttaatac tggcgtgact ttaaaaaaaa gcaaaagaaa cttggaattc
      301 gcttcgctca taaaactttt ttactcgcta cgctcgatcc aggagcaaat ttctgcacga
      361 aacttacgct ttcttaggct aaagaggaca aaaaaatgtt tttcagaggg tctaggcgca
      421 aattttgata gttcgctcgt agacactcgc tctatgcatc tatctaaagt caaaatacct
      481 ctttatttcg ctctcatttg cgttttttga gctgttttgt gtttttcact atgagatcta
      541 tgttttctag gtaaaggctc ttaaatcgca tttgaacgcg ttctatgaag cttttcataa
      601 ttaaagtttt gggttgaaat ttttaacctg gatgaatgga caaaaaattg attttttacg
      661 tttttctaaa caaggaaata aatacttttt tccatgcaaa accaagcacc ggccctacgt
      721 cactttagcc aattttccaa tccaagatcc ggcacatgcc gggatttttt tgtcacacac
      781 cacgcatcta acgatgacac ccctcaaagc cttacaggat aaggatttca gcgatttttt
      841 agggtctcag aaacgtgtac agaaaaatgg tcttttttta gaataagtga ttgaattatc
      901 tattttttat gctgtgctca tggttgtaca tcaaaacaac acttttttta gataagatat
      961 tgataaattt aagaaaaaat atgtactcat gattgtacat aaaaaaagca ttatatttaa
     1021 ataagttttt gttttagaat aaattaattc tgtacaaata agtttgtacg taatctaatt
     1081 attatttttt atttaattag ttgaattata tatatttaat tttgtacaaa atctaatttt
     1141 aataagaggt gtaatttatg ggcgaaaaag tcaaaactta tgcagtcagg ttagatccac
     1201 aagtagcaga attctacgat caattggcta attcagaagg aatcagaacc agtaaattat
     1261 tcagtaaaat tttgaccaat gattttcaat ctattgctgt taaacagcaa gccgatcgta
     1321 ttgaggatct ggtgaatcga ttagaaaagc gaatagatga ttttgaggaa gataacaatt
     1381 tttctgaaaa atactatgaa gacttttctg gactatattt catgatgttg tggctcttaa
     1441 tgaaaaatgg cgcttctaaa gatgaggtta gaatgataca agctaaaggt tattcatatg
     1501 cagatgataa ctataagagg gtaaagtagt gtcagataat attattcagc tttcaaagcc
     1561 atgtacgctt tgtgaaaata gacaaaatgt acaacttttt gcaggattga tgctgtgtgg
     1621 aagttgtcag gaaaatatca ggcttacgaa tccaagtatg ttctcggcca atgatgagat
     1681 tgaacaaaaa gcccagaatt aacctgggct tcgtataaga gattttatgt taaatgccga
     1741 actgaattaa aaatagaaat gtagttgagc tgatgcttgt tcatttcttt atttttagta
     1801 attgaggtga acatactgtc agtacatggt agttataagt aacgtgtaac tttgtttgca
     1861 caaagatttg agttatgcca agtgatggat tgatttgtac ttaaaatttt aagcttatcc
     1921 acttgctttg caagccacta cactttattt acaatcctat gttgtggaag taagggatct
     1981 tgtactcgcc cagtatgttc aacttgatta tatctaaagg actattaggc ttcaatcaaa
     2041 tcaattttcc aattagtcga ctgaattaac acgtttaaat cacgtaattt aagactaata
     2101 tcatcagctt gtttttgtaa tgatgaaact ggaataactt tatgccactt tatttcccgc
     2161 gtactataac gatcagattc acgagatgta ttacttattg ctgcagttag ggttttgtgc
     2221 tgttctacaa attcatcacg caaagtcaat aaagcaagta gagaacggcc atcatttaat
     2281 tttgttaaag cattagtttc atgaattgca taacttaatt tttgaaattc ttgaattaaa
     2341 gaaaatactt gttttagtag ttcatttgga tcttcagatg gttgatcacc atcttgtacc
     2401 aatacatttg cattgatacg ttgttttagt gaaataattt ttttttgttg atcacttcga
     2461 agtaagagag cttctgctaa tttcattttt cacctattaa ccaattagaa ttaagtttta
     2521 aacagcttat atcctcattc tttaaagatc aaaaaaattg ctttttatct agctaaaagg
     2581 tgtctacaaa atcggtggct attcagtgct gaaaaggctg ccgacacata aagtgaccca
     2641 gattgaagag ttactgccac actgctggaa acctaaatcg aattaaaaaa taggtatggg
     2701 attcagcgga cgcttacctt aattattaat ctaatcattt tataagaaaa atcttctaag
     2761 aaattttctt agaagattac agtatcttat ctagaaatta agatttaagc ctaaactaaa
     2821 atttcttcca atttggggaa tatatggcaa aaatgtttca tgggcataga ctttctgatc
     2881 aagtaaatta ttcgctttaa agaataaatc atattctgtg tgagatagct tatttttata
     2941 tgctaaagtc atattaagca tattataacc tgatgtaacc tgctcaaatt tagaaatatt
     3001 atcttgttta aagactcgat aatattctat ttcaccttta agcgattcat caaaatcagc
     3061 ttttagtcgt gtacccaaac gagcaggagg taatctagga gtgtaacgat ccttttgggg
     3121 agctaaagtc ggttctctat tccagatatc gtatgcaatt acagcatcag gtaaatttgt
     3181 taaacggcct ttcacataat ctccaaaaag agagccgtga taaacagaat taaattgata
     3241 ccctatgttg ccctctaatc cataaaaatt agcagcagac tggctataat gattaatacg
     3301 tagtgtatgc tgatctctaa cttttgtagt tcctaaaact tcattgagtg tctgtaaata
     3361 tatatagtta tcaaaatcat aatagtaagt actgatttga taatcaagta aatcatcctt
     3421 ataggccaga ctgatttcta aattattgga tttctctttt cttagaaagc ggttacctat
     3481 ttcaaaagaa ttagtagcga gatgcatacc atgagtatac atttcttgtg cattaggtaa
     3541 tctttcttga tgtgtacctg ttaatgataa gatataattt ggagcaaatc tccagtggac
     3601 tgtacctgca taagaaaaag ctgtttcttt gttaggttga acagcttcta aaatagattt
     3661 taaattttga gccctgattt tattattttt ttctacataa ggactgttat atttattagg
     3721 ccaaggtttc attgaatctt taattttttc tatatcataa tccatactaa ctttctgctt
     3781 ttctatacga gcacctagtt cgaaagtaaa gtcgttccag ttatattttt ctaggccaaa
     3841 tacactaaag tttttagtga catttcgatt taatagctgc tgattatcta gataggtggt
     3901 atggccttct tgagaatgga ctggtgataa agcactatta tcctgttcca aatattgtag
     3961 ccccaagata ccggttaatt cccctaatgg ctgatgtgat aactctatgc ggcctacttt
     4021 tcctgtattt ttaaaaaagt tagtaatctt atctccctcg agttcattgt gaaaataatc
     4081 tatataactc aatgaggtac gaattttgtc aataccggta aatggttgag tgaattctcc
     4141 acgcacatca taacgtcgag tatttaaatc aatataagga gcattatgag agaaattagt
     4201 ttcatgaata tgattatgct gatgacaatc aggacgtgga tttatgtaat taatatcttg
     4261 ctcatccatc aattgaggat aatataaaag ataatttttt aatccactaa ttcgtgaatc
     4321 gataccgatt gcagaggcac cacaaccttc atataaatgg ctatgggctg gtaaaccata
     4381 ttcatcatga cgatgtgtat aagaaactcc taaataagta ttatcgtcta tccaggataa
     4441 tccaagcgta cctactctcg actctgccca gctttctggt aaatatttta aatttttata
     4501 aatactgttt tggttttcaa gatcttctaa agaattataa gagctagact ggtaatgagg
     4561 agttttatag ttaccagcat tacgatataa cccttctgcc cgtaaagcta catgtgggct
     4621 taagccaact gttacacctg ctgtggtcaa cttctcatta ctgccagtat taaaacgtaa
     4681 tgtagtatta ccctctaatc cgtcttgagg catttgggtt ggaattttat agtcaatgac
     4741 attgactacc cctgccgcac tatttgaact atacaaaagg gtacttgcac cacgtataat
     4801 ttcaatagac ttagctaaac ttggttcaac tgtgacagcg tgatctgggg acatattaga
     4861 catatccaac acgtcagcat tattctgtaa aactttaata cgttttcctt cctgcccacg
     4921 aataatagga gtagatgctc cacctccata ttgattagaa tgaattccta attccgtacc
     4981 caaggcatcc cctagagttg tcgaacgctg tttaagttta ctagtatcaa taaactgatt
     5041 actaacattt agacttttgc ctaacagctc agaccctaac tcatcagatt gtgcagttaa
     5101 tactattgta tttaaggtag taacaggccc ttcagctgcc aataaaattt tcgaggcccc
     5161 taaagtaatt aaagctaaaa atagtttact tttatttagc ataaaacaac caaatttaat
     5221 gttatattat aatataacac ttaaaaattt ataaaatcta ctatttaaat ttttgaatgg
     5281 taatcccccc taaaaatgaa gtcaaaaatg ggagcctaag ctcccattct tattgatgat
     5341 tttttaattc ttttagcata gcatctcgta aaatttcatt catacgtgtt tgataacctt
     5401 tgccttgtgc tttgaaccaa gcaagtacat cagcatctaa ccgaattgaa gtttgttgct
     5461 ttactgggcg gtaaaattga ttttggcgta cagcattgct ccagtctgta atttcaggaa
     5521 tatctgatag atctagctga tcatcaggaa ccgtgccttt agcaagcaag cgttgaattt
     5581 cagcatcttg cttctcacca aatttctcat tcagctcttt gtgtgagtat ctaaccatgc
     5641 tcatatttgt ttcgctccgc tttagtgact tgccttgcac taatgattcg gatgatttca
     5701 cagtcatctt catcaaaaat ggtgtgagcc accaataaca ttagtacgcc tttaactcgt
     5761 ccaatggttt gccaacgttc ttcaccattg gtatgtctgt cttggattga gatccgtaat
     5821 ggatcttcaa aaacaaggct tgcagtttcg aaagagatat catgcttttt ctgattcttt
     5881 cgattcttag cctcatccca ttcaaaatac tgttccataa aaaacattca aatttgtata
     5941 tacaataatg tatcacaaaa ttgtatcact caaccagatt cgtgtaattt ttagtcaaaa
     6001 ttattggcaa gaagtatcaa aactgctcat aaaaaagccg acttgtttca aagtcggctt
     6061 tttgggcatt ttgggatttt tctattttta ttaagtagtt gatatttata ttaatataca
     6121 agtgtacttc gtataatcgc cattatgtta aatagagata agacatactc taattttaga
     6181 gttttctttt aggtatgata taaaaatata caagcctaac agtttagttt aaaggttaaa
     6241 aattgaataa attaacaaat atttttatgg ctttaggagt ttgcctttca atggaaacat
     6301 ttgcagaacc tgttttttct aatgatttat tagcgaaagc agaaaatgga gatacttctg
     6361 ctcagttaga attagccgag atttatctat atggtcatgg tgttgattca gatgaaaatc
     6421 aagctgaagt ttgggctctt aaatcagcag aaaatggaaa tgtagcagca atgttttggt
     6481 tagctgatgg atatgttact tatgctagat taatggaaga tgatgacaaa aacgattctt
     6541 tagaacattt ccaaaaagct tttaagtggt ttcagaaagc ctcagaaaat ggccattctg
     6601 aatcaatggt tgagttagct gatctatata ctcgtgcaga tagcggaata gaagttaata
     6661 ttaataaagc ttttgaactt cgtgaaaagg ctgcaaagtt aggtaataag aaagcaatgc
     6721 gaagtctttc cgttatgtat cgtgatggta taggtattcc taaaaatact gatttagctc
     6781 aaagttggtg ggataagtct gaaaattagt tttaatattt cctaaaatta acataataca
     6841 ccttatacga aatgctgtat aattatcctt ataattcaac gctgtagttt ctagtttata
     6901 gcccgagcat ccctgttcgg gcttttttta tgacttttca cgttccacgc tattatttca
     6961 tcaatgttcc acacttttga ttagctcaat tgaataatca ggatgcctgg tctttttctt
     7021 tttggcattc atccagctct ttcttcagcg cctttttatc ctggtacgat gcaatcagca
     7081 tgatcgggat cagcagcgga gctacaataa ttcccagggc aatgtctttc agtttcggca
     7141 tgatctttca cccttattgc tcagtcatta tcaaagacgg cattcagcac acggccaaat
     7201 aaaccacgtt ttttgtgttg tggcactgcc acactttgca acgtggcatg ctgtagcgtt
     7261 tgggtgtggt atgccgtagt ttcttgctgt ggcactgcta cattttctgc aatatttaat
     7321 tcattattct ttttgttatc agtgtcttgt tgtgttgtcg catgctgtgg catcttggtg
     7381 tcgcatgccg tagcatcttg gtgtggtgct gctgtggcag gtgcaacact tttcagctca
     7441 atgagtgttt gcatacggtc aatttgttgc attaaccgtt cttcacgttc ttggtatttt
     7501 ttgacttcat tttctaaatg ggcaataagt ttttttgtag attcatcacc ttcttgctta
     7561 gatgattcaa ttttttgaga tgtttgctca ggttctccaa agcatcgtat gacctctgct
     7621 agatcgaatt gtccatcact ggagcgtgat aaacgtccat tacgtacagc tctgtaaatg
     7681 gttgatctat ccacatgaaa ttttttagat acttcactta gtgataactt agccatttac
     7741 ggtgtccttt ttatttgatg gcatgtatcc cacttttttt aggtatgggt atagctcttt
     7801 gaattgagcg ggatcttgta gcatctcagc aatacgtaca gcaaactgtt ggtagctttc
     7861 tgttccttgt gaatagcgac ccatttcagg gagttctgaa agtttatttg caaacatatg
     7921 ccgttgagca tcggtcatct ttgaaaaaag ctccaaagtg ttcggatctc tttctattga
     7981 tgggctatcc ttcttcttct gtttaaaggt aaaagaaaat cctgaaatag aacgtcctct
     8041 tttatgctgt tcatatttta cagttatatc cgtatgctca tttatttgtt taattgaaag
     8101 ctctaaaaca cgctctttta aatgagccat ccgtaaatat tcattatcaa gaacgcctaa
     8161 cttctttcta aattcttgta gttctatggt tggggtttta ccagtgcttc gccactgaat
     8221 tagaagctca tataagcgaa tggcataagc gctactaaga ctactaactt gctgtaattc
     8281 atatttagta aattgttctt ccagtcgggt tatgaaggga acaacagcag gtgcaaatat
     8341 taagtctaca gttgcttcat tgtcgttata agcaatttga gatacccaac gactcataac
     8401 atttcggata ttcccattag cttttttctc ttgatagcta aattgacgcg cgaataaatc
     8461 cttacaagca tcttttaagg cttgataagc tgtattacgg tgtacaccaa attgattgat
     8521 ataactatct gcatgaactt ctaatggatc atttgcatta atccctttcc cactttccct
     8581 tgcttcaaca atagccaata aaattaaacg ttgttctact aagtctaagt tatagcttgc
     8641 gttgattaag gcattgtcct ttacaactaa atctctcata gccactattt tttattgtgt
     8701 ataagtacac gttaatatta tttgtgtact a
//
