LOCUS       NZ_CP104906             8731 bp    DNA     circular CON 12-OCT-2023
DEFINITION  Acinetobacter baumannii strain Ab-3557 plasmid punamed1, complete
            sequence.
ACCESSION   NZ_CP104906
VERSION     NZ_CP104906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30929296
            Assembly: GCF_025449155.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Yang,Y.
  TITLE     Alteration of adeS contributes to tigecycline resistance and
            collateral sensitivity to sulbactam in Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Yang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2022) Department of Clinical Laboratory,
            Affiliated Hangzhou First People's hospital, NO.4 Xueshi Road,
            Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/12/2023 03:05:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,706
            Genes (coding)                    :: 3,638
            CDSs (with protein)               :: 3,638
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 19 of 68
            Pseudo Genes (incomplete)         :: 40 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104906.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="Ab-3557"
                     /db_xref="taxon:470"
                     /plasmid="punamed1"
                     /collection_date="2017-03"
     gene            complement(join(6596..8731,1..276))
                     /gene="znuD2"
                     /locus_tag="N7E62_RS00005"
                     /old_locus_tag="N7E62_00005"
     CDS             complement(join(6596..8731,1..276))
                     /gene="znuD2"
                     /locus_tag="N7E62_RS00005"
                     /old_locus_tag="N7E62_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(404..718)
                     /locus_tag="N7E62_RS00010"
                     /old_locus_tag="N7E62_00010"
     CDS             complement(404..718)
                     /locus_tag="N7E62_RS00010"
                     /old_locus_tag="N7E62_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(705..992)
                     /locus_tag="N7E62_RS00015"
                     /old_locus_tag="N7E62_00015"
     CDS             complement(705..992)
                     /locus_tag="N7E62_RS00015"
                     /old_locus_tag="N7E62_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            1365..1883
                     /locus_tag="N7E62_RS00020"
                     /old_locus_tag="N7E62_00020"
     CDS             1365..1883
                     /locus_tag="N7E62_RS00020"
                     /old_locus_tag="N7E62_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            complement(2072..2215)
                     /locus_tag="N7E62_RS00025"
                     /old_locus_tag="N7E62_00025"
     CDS             complement(2072..2215)
                     /locus_tag="N7E62_RS00025"
                     /old_locus_tag="N7E62_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2235..2810)
                     /locus_tag="N7E62_RS00030"
                     /old_locus_tag="N7E62_00030"
     CDS             complement(2235..2810)
                     /locus_tag="N7E62_RS00030"
                     /old_locus_tag="N7E62_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2803..3753)
                     /gene="repM"
                     /locus_tag="N7E62_RS00035"
                     /old_locus_tag="N7E62_00035"
     CDS             complement(2803..3753)
                     /gene="repM"
                     /locus_tag="N7E62_RS00035"
                     /old_locus_tag="N7E62_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            4437..4691
                     /locus_tag="N7E62_RS19030"
     CDS             4437..4691
                     /locus_tag="N7E62_RS19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            4963..5334
                     /locus_tag="N7E62_RS00040"
                     /old_locus_tag="N7E62_00040"
     CDS             4963..5334
                     /locus_tag="N7E62_RS00040"
                     /old_locus_tag="N7E62_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            5334..5507
                     /locus_tag="N7E62_RS00045"
                     /old_locus_tag="N7E62_00045"
     CDS             5334..5507
                     /locus_tag="N7E62_RS00045"
                     /old_locus_tag="N7E62_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(5830..6291)
                     /locus_tag="N7E62_RS00050"
                     /old_locus_tag="N7E62_00050"
     CDS             complement(5830..6291)
                     /locus_tag="N7E62_RS00050"
                     /old_locus_tag="N7E62_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     Typing          complement(2804..3753)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggagtagat gctccacctc catattgatt agaatgaatt cctaattccg tacccaaggc
       61 atcccctaga gttgtcgaac gctgtttaag tttactagta tcaataaact gattactaac
      121 atttagactt ttgcctaaca gctcagaccc taactcatca gattgtgcag ttaatactat
      181 tgtatttaag gtagtaacag gcccttcagc tgccaataaa attttcgagg cccctaaagt
      241 aattaaagct aaaaatagtt tacttttatt tagcataaaa caaccaaatt taatgttata
      301 ttataatata acacttaaaa atttataaaa tctactattt aaatttttga atggtaatcc
      361 cccctaaaaa tgaagtcaaa aatgggagcc taagctccca ttcttattga tgatttttta
      421 attcttttag catagcatct cgtaaaattt cattcatacg tgtttgataa cctttgcctt
      481 gtgctttgaa ccaagcaagt acatcagcat ctaaccgaat tgaagtttgt tgctttactg
      541 ggcggtaaaa ttgattttgg cgtacagcat tgctccagtc tgtaatttca ggaatatctg
      601 atagatctag ctgatcatca ggaaccgtgc ctttagcaag caagcgttga atttcagcat
      661 cttgcttctc accaaatttc tcattcagct ctttgtgtga gtatctaacc atgctcatat
      721 ttgtttcgct ccgctttagt gacttgcctt gcactaatga ttcggatgat ttcacagtca
      781 tcttcatcaa aaatggtgtg agccaccaat aacattagta cgcctttaac tcgtccaatg
      841 gtttgccaac gttcttcacc attggtatgt ctgtcttgga ttgagatccg taatggatct
      901 tcaaaaacaa ggcttgcagt ttcgaaagag atatcatgct ttttctgatt ctttcgattc
      961 ttagcctcat cccattcaaa atactgttcc ataaaaaaca ttcaaatttg tatatacaat
     1021 aatgtatcac aaaattgtat cactcaacca gattcgtgta atttttagtc aaaattattg
     1081 gcaagaagta tcaaaactgc tcataaaaaa gccgacttgt ttcaaagtcg gctttttggg
     1141 cattttggga tttttctatt tttattaagt agttgatatt tatattaata tacaagtgta
     1201 cttcgtataa tcgccattat gttaaataga gataagacat actctaattt tagagttttc
     1261 ttttaggtat gatataaaaa tatacaagcc taacagttta gtttaaaggt taaaaattga
     1321 ataaattaac aaatattttt atggctttag gagtttgcct ttcaatggaa acatttgcag
     1381 aacctgtttt ttctaatgat ttattagcga aagcagaaaa tggagatact tctgctcagt
     1441 tagaattagc cgagatttat ctatatggtc atggtgttga ttcagatgaa aatcaagctg
     1501 aagtttgggc tcttaaatca gcagaaaatg gaaatgtagc agcaatgttt tggttagctg
     1561 atggatatgt tacttatgct agattaatgg aagatgatga caaaaacgat tctttagaac
     1621 atttccaaaa agcttttaag tggtttcaga aagcctcaga aaatggccat tctgaatcaa
     1681 tggttgagtt agctgatcta tatactcgtg cagatagcgg aatagaagtt aatattaata
     1741 aagcttttga acttcgtgaa aaggctgcaa agttaggtaa taagaaagca atgcgaagtc
     1801 tttccgttat gtatcgtgat ggtataggta ttcctaaaaa tactgattta gctcaaagtt
     1861 ggtgggataa gtctgaaaat tagttttaat atttcctaaa attaacataa tacaccttat
     1921 acgaaatgct gtataattat ccttataatt caacgctgta gtttctagtt tatagcccga
     1981 gcatccctgt tcgggctttt tttatgactt ttcacgttcc acgctattat ttcatcaatg
     2041 ttccacactt ttgattagct caattgaata atcaggatgc ctggtctttt tctttttggc
     2101 attcatccag ctctttcttc agcgcctttt tatcctggta cgatgcaatc agcatgatcg
     2161 ggatcagcag cggagctaca ataattccca gggcaatgtc tttcagtttc ggcatgatct
     2221 ttcaccctta ttgctcagtc attatcaaag acggcattca gcacacggcc aaataaacca
     2281 cgttttttgt gttgtggcac tgccacactt tgcaacgtgg catgctgtag cgtttgggtg
     2341 tggtatgccg tagtttcttg ctgtggcact gctacatttt ctgcaatatt taattcatta
     2401 ttctttttgt tatcagtgtc ttgttgtgtt gtcgcatgct gtggcatctt ggtgtcgcat
     2461 gccgtagcat cttggtgtgg tgctgctgtg gcaggtgcaa cacttttcag ctcaatgagt
     2521 gtttgcatac ggtcaatttg ttgcattaac cgttcttcac gttcttggta ttttttgact
     2581 tcattttcta aatgggcaat aagttttttt gtagattcat caccttcttg cttagatgat
     2641 tcaatttttt gagatgtttg ctcaggttct ccaaagcatc gtatgacctc tgctagatcg
     2701 aattgtccat cactggagcg tgataaacgt ccattacgta cagctctgta aatggttgat
     2761 ctatccacat gaaatttttt agatacttca cttagtgata acttagccat ttacggtgtc
     2821 ctttttattt gatggcatgt atcccacttt ttttaggtat gggtatagct ctttgaattg
     2881 agcgggatct tgtagcatct cagcaatacg tacagcaaac tgttggtagc tttctgttcc
     2941 ttgtgaatag cgacccattt cagggagttc tgaaagttta tttgcaaaca tatgccgttg
     3001 agcatcggtc atctttgaaa aaagctccaa agtgttcgga tctctttcta ttgatgggct
     3061 atccttcttc ttctgtttaa aggtaaaaga aaatcctgaa atagaacgtc ctcttttatg
     3121 ctgttcatat tttacagtta tatccgtatg ctcatttatt tgtttaattg aaagctctaa
     3181 aacacgctct tttaaatgag ccatccgtaa atattcatta tcaagaacgc ctaacttctt
     3241 tctaaattct tgtagttcta tggttggggt tttaccagtg cttcgccact gaattagaag
     3301 ctcatataag cgaatggcat aagcgctact aagactacta acttgctgta attcatattt
     3361 agtaaattgt tcttccagtc gggttatgaa gggaacaaca gcaggtgcaa atattaagtc
     3421 tacagttgct tcattgtcgt tataagcaat ttgagatacc caacgactca taacatttcg
     3481 gatattccca ttagcttttt tctcttgata gctaaattga cgcgcgaata aatccttaca
     3541 agcatctttt aaggcttgat aagctgtatt acggtgtaca ccaaattgat tgatataact
     3601 atctgcatga acttctaatg gatcatttgc attaatccct ttcccacttt cccttgcttc
     3661 aacaatagcc aataaaatta aacgttgttc tactaagtct aagttatagc ttgcgttgat
     3721 taaggcattg tcctttacaa ctaaatctct catagccact attttttatt gtgtataagt
     3781 acacgttaat attatttgtg tactaatgca aggtaaacgt ggacatattg caaggtaaac
     3841 gtggacatat tgcaaggtaa acgtggacat attgcaaggt aaacgtggac atataagagc
     3901 taaaaccctt tattcataag gctttcaagc catttaaaag tatttaaaaa ctttaaaata
     3961 attaaaagcc cagggaatga aaaattgccc ttggttttcg cttcgctcaa actctattga
     4021 acttcgcttt cgctcagttc gttggggcaa ttttttggtt aatactggcg tgactttaaa
     4081 aaaaagcaaa agaaacttgg aattcgcttc gctcataaaa cttttttact cgctacgctc
     4141 gatccaggag caaatttctg cacgaaactt acgctttctt aggctaaaga ggacaaaaaa
     4201 atgtttttca gagggtctag gcgcaaattt tgatagttcg ctcgtagaca ctcgctctat
     4261 gcatctatct aaagtcaaaa tacctcttta tttcgctctc atttgcgttt tttgagctgt
     4321 tttgtgtttt tcactatgag atctatgttt tctaggtaaa ggctcttaaa tcgcatttga
     4381 acgcgttcta tgaagctttt cataattaaa gttttgggtt gaaattttta acctggatga
     4441 atggacaaaa aattgatttt ttacgttttt ctaaacaagg aaataaatac ttttttccat
     4501 gcaaaaccaa gcaccggccc tacgtcactt tagccaattt tccaatccaa gatccggcac
     4561 atgccgggat ttttttgtca cacaccacgc atctaacgat gacacccctc aaagccttac
     4621 aggataagga tttcagcgat tttttagggt ctcagaaacg tgtacagaaa aatggtcttt
     4681 ttttagaata agtgattgaa ttatctattt tttatgctgt gctcatggtt gtacatcaaa
     4741 acaacacttt ttttagataa gatattgata aatttaagaa aaaatatgta ctcatgattg
     4801 tacataaaaa aagcattata tttaaataag tttttgtttt agaataaatt aattctgtac
     4861 aaataagttt gtacgtaatc taattattat tttttattta attagttgaa ttatatatat
     4921 ttaattttgt acaaaatcta attttaataa gaggtgtaat ttatgggcga aaaagtcaaa
     4981 acttatgcag tcaggttaga tccacaagta gcagaattct acgatcaatt ggctaattca
     5041 gaaggaatca gaaccagtaa attattcagt aaaattttga ccaatgattt tcaatctatt
     5101 gctgttaaac agcaagccga tcgtattgag gatctggtga atcgattaga aaagcgaata
     5161 gatgattttg aggaagataa caatttttct gaaaaatact atgaagactt ttctggacta
     5221 tatttcatga tgttgtggct cttaatgaaa aatggcgctt ctaaagatga ggttagaatg
     5281 atacaagcta aaggttattc atatgcagat gataactata agagggtaaa gtagtgtcag
     5341 ataatattat tcagctttca aagccatgta cgctttgtga aaatagacaa aatgtacaac
     5401 tttttgcagg attgatgctg tgtggaagtt gtcaggaaaa tatcaggctt acgaatccaa
     5461 gtatgttctc ggccaatgat gagattgaac aaaaagccca gaattaacct gggcttcgta
     5521 taagagattt tatgttaaat gccgaactga attaaaaata gaaatgtagt tgagctgatg
     5581 cttgttcatt tctttatttt tagtaattga ggtgaacata ctgtcagtac atggtagtta
     5641 taagtaacgt gtaactttgt ttgcacaaag atttgagtta tgccaagtga tggattgatt
     5701 tgtacttaaa attttaagct tatccacttg ctttgcaagc cactacactt tatttacaat
     5761 cctatgttgt ggaagtaagg gatcttgtac tcgcccagta tgttcaactt gattatatct
     5821 aaaggactat taggcttcaa tcaaatcaat tttccaatta gtcgactgaa ttaacacgtt
     5881 taaatcacgt aatttaagac taatatcatc agcttgtttt tgtaatgatg aaactggaat
     5941 aactttatgc cactttattt cccgcgtact ataacgatca gattcacgag atgtattact
     6001 tattgctgca gttagggttt tgtgctgttc tacaaattca tcacgcaaag tcaataaagc
     6061 aagtagagaa cggccatcat ttaattttgt taaagcatta gtttcatgaa ttgcataact
     6121 taatttttga aattcttgaa ttaaagaaaa tacttgtttt agtagttcat ttggatcttc
     6181 agatggttga tcaccatctt gtaccaatac atttgcattg atacgttgtt ttagtgaaat
     6241 aatttttttt tgttgatcac ttcgaagtaa gagagcttct gctaatttca tttttcacct
     6301 attaaccaat tagaattaag ttttaaacag cttatatcct cattctttaa agatcaaaaa
     6361 aattgctttt tatctagcta aaaggtgtct acaaaatcgg tggctattca gtgctgaaaa
     6421 ggctgccgac acataaagtg acccagattg aagagttact gccacactgc tggaaaccta
     6481 aatcgaatta aaaaataggt atgggattca gcggacgctt accttaatta ttaatctaat
     6541 cattttataa gaaaaatctt ctaagaaatt ttcttagaag attacagtat cttatctaga
     6601 aattaagatt taagcctaaa ctaaaatttc ttccaatttg gggaatatat ggcaaaaatg
     6661 tttcatgggc atagactttc tgatcaagta aattattcgc tttaaagaat aaatcatatt
     6721 ctgtgtgaga tagcttattt ttatatgcta aagtcatatt aagcatatta taacctgatg
     6781 taacctgctc aaatttagaa atattatctt gtttaaagac tcgataatat tctatttcac
     6841 ctttaagcga ttcatcaaaa tcagctttta gtcgtgtacc caaacgagca ggaggtaatc
     6901 taggagtgta acgatccttt tggggagcta aagtcggttc tctattccag atatcgtatg
     6961 caattacagc atcaggtaaa tttgttaaac ggcctttcac ataatctcca aaaagagagc
     7021 cgtgataaac agaattaaat tgatacccta tgttgccctc taatccataa aaattagcag
     7081 cagactggct ataatgatta atacgtagtg tatgctgatc tctaactttt gtagttccta
     7141 aaacttcatt gagtgtctgt aaatatatat agttatcaaa atcataatag taagtactga
     7201 tttgataatc aagtaaatca tccttatagg ccagactgat ttctaaatta ttggatttct
     7261 cttttcttag aaagcggtta cctatttcaa aagaattagt agcgagatgc ataccatgag
     7321 tatacatttc ttgtgcatta ggtaatcttt cttgatgtgt acctgttaat gataagatat
     7381 aatttggagc aaatctccag tggactgtac ctgcataaga aaaagctgtt tctttgttag
     7441 gttgaacagc ttctaaaata gattttaaat tttgagccct gattttatta tttttttcta
     7501 cataaggact gttatattta ttaggccaag gtttcattga atctttaatt ttttctatat
     7561 cataatccat actaactttc tgcttttcta tacgagcacc tagttcgaaa gtaaagtcgt
     7621 tccagttata tttttctagg ccaaatacac taaagttttt agtgacattt cgatttaata
     7681 gctgctgatt atctagatag gtggtatggc cttcttgaga atggactggt gataaagcac
     7741 tattatcctg ttccaaatat tgtagcccca agataccggt taattcccct aatggctgat
     7801 gtgataactc tatgcggcct acttttcctg tatttttaaa aaagttagta atcttatctc
     7861 cctcgagttc attgtgaaaa taatctatat aactcaatga ggtacgaatt ttgtcaatac
     7921 cggtaaatgg ttgagtgaat tctccacgca catcataacg tcgagtattt aaatcaatat
     7981 aaggagcatt atgagagaaa ttagtttcat gaatatgatt atgctgatga caatcaggac
     8041 gtggatttat gtaattaata tcttgctcat ccatcaattg aggataatat aaaagataat
     8101 tttttaatcc actaattcgt gaatcgatac cgattgcaga ggcaccacaa ccttcatata
     8161 aatggctatg ggctggtaaa ccatattcat catgacgatg tgtataagaa actcctaaat
     8221 aagtattatc gtctatccag gataatccaa gcgtacctac tctcgactct gcccagcttt
     8281 ctggtaaata ttttaaattt ttataaatac tgttttggtt ttcaagatct tctaaagaat
     8341 tataagagct agactggtaa tgaggagttt tatagttacc agcattacga tataaccctt
     8401 ctgcccgtaa agctacatgt gggcttaagc caactgttac acctgctgtg gtcaacttct
     8461 cattactgcc agtattaaaa cgtaatgtag tattaccctc taatccgtct tgaggcattt
     8521 gggttggaat tttatagtca atgacattga ctacccctgc cgcactattt gaactataca
     8581 aaagggtact tgcaccacgt ataatttcaa tagacttagc taaacttggt tcaactgtga
     8641 cagcgtgatc tggggacata ttagacatat ccaacacgtc agcattattc tgtaaaactt
     8701 taatacgttt tccttcctgc ccacgaataa t
//
