LOCUS       NZ_CP104839            10802 bp    DNA     circular CON 10-OCT-2023
DEFINITION  Enterobacter cloacae strain 2022CK-00409 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP104839
VERSION     NZ_CP104839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29981189
            Assembly: GCF_025426135.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00409
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Medaka v. 1.6.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/10/2023 04:30:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,856
            CDSs (total)                      :: 5,741
            Genes (coding)                    :: 5,442
            CDSs (with protein)               :: 5,442
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 299
            CDSs (without protein)            :: 299
            Pseudo Genes (ambiguous residues) :: 0 of 299
            Pseudo Genes (frameshifted)       :: 76 of 299
            Pseudo Genes (incomplete)         :: 202 of 299
            Pseudo Genes (internal stop)      :: 48 of 299
            Pseudo Genes (multiple problems)  :: 25 of 299
            Pseudo Genes (short protein)      :: 1 of 299
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00409"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022-06-28"
     gene            complement(505..972)
                     /gene="traD"
                     /locus_tag="N7S94_RS25650"
                     /old_locus_tag="N7S94_25645"
     CDS             complement(505..972)
                     /gene="traD"
                     /locus_tag="N7S94_RS25650"
                     /old_locus_tag="N7S94_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_261659444.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     gene            1138..2235
                     /gene="mobA"
                     /locus_tag="N7S94_RS25655"
                     /old_locus_tag="N7S94_25650"
     CDS             1138..2235
                     /gene="mobA"
                     /locus_tag="N7S94_RS25655"
                     /old_locus_tag="N7S94_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_261659445.1"
                     /translation="MASYHLSVKNGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            2248..2832
                     /locus_tag="N7S94_RS25660"
                     /old_locus_tag="N7S94_25655"
     CDS             2248..2832
                     /locus_tag="N7S94_RS25660"
                     /old_locus_tag="N7S94_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(<3134..3919)
                     /locus_tag="N7S94_RS25665"
                     /old_locus_tag="N7S94_25660"
                     /pseudo=""
     CDS             complement(<3134..3919)
                     /locus_tag="N7S94_RS25665"
                     /old_locus_tag="N7S94_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611225.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(4216..>4533)
                     /locus_tag="N7S94_RS25670"
                     /old_locus_tag="N7S94_25665"
                     /pseudo=""
     CDS             complement(4216..>4533)
                     /locus_tag="N7S94_RS25670"
                     /old_locus_tag="N7S94_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016707037.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(4783..5088)
                     /locus_tag="N7S94_RS25675"
                     /old_locus_tag="N7S94_25670"
     CDS             complement(4783..5088)
                     /locus_tag="N7S94_RS25675"
                     /old_locus_tag="N7S94_25670"
                     /inference="COORDINATES: protein motif:HMM:NF024592.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="WP_261659446.1"
                     /translation="MNKTKGCLIANFATVPYLASAVSGLTELDAIALSTLRLFGNGKLG
                     AEVAANAIGLAILSNLCFKTGLLAWVAGRDFLLRCLPGMMAIGIGIAIGMGWVGYA"
                     /name="DUF4010 domain-containing protein"
     gene            complement(5094..5798)
                     /locus_tag="N7S94_RS25680"
                     /old_locus_tag="N7S94_25675"
     CDS             complement(5094..5798)
                     /locus_tag="N7S94_RS25680"
                     /old_locus_tag="N7S94_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <5860..6030
                     /locus_tag="N7S94_RS25685"
                     /old_locus_tag="N7S94_25680"
                     /pseudo=""
     CDS             <5860..6030
                     /locus_tag="N7S94_RS25685"
                     /old_locus_tag="N7S94_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            6204..7829
                     /locus_tag="N7S94_RS25690"
                     /old_locus_tag="N7S94_25685"
     CDS             6204..7829
                     /locus_tag="N7S94_RS25690"
                     /old_locus_tag="N7S94_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-4.3"
                     /protein_id="WP_011638903.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVDP
                     LFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMIEN
                     TFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLMFVG
                     IGIVAFFYYQDYAAFGRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGLDAKN
                     ASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGTATAVS
                     LPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVENLTIDLK
                     SDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPPEHRKFIP
                     DCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYLSDHGESLG
                     EKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGGFSHDNLFDS
                     LLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="phosphoethanolamine--lipid A transferase MCR-4.3"
     gene            complement(8167..8454)
                     /locus_tag="N7S94_RS25695"
                     /old_locus_tag="N7S94_25690"
     CDS             complement(8167..8454)
                     /locus_tag="N7S94_RS25695"
                     /old_locus_tag="N7S94_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_172691904.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(8447..8764)
                     /locus_tag="N7S94_RS25700"
                     /old_locus_tag="N7S94_25695"
     CDS             complement(8447..8764)
                     /locus_tag="N7S94_RS25700"
                     /old_locus_tag="N7S94_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_040113353.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9011..9460)
                     /locus_tag="N7S94_RS25705"
                     /old_locus_tag="N7S94_25700"
     CDS             complement(9011..9460)
                     /locus_tag="N7S94_RS25705"
                     /old_locus_tag="N7S94_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016517644.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9468..10424)
                     /locus_tag="N7S94_RS25710"
                     /old_locus_tag="N7S94_25705"
     CDS             complement(9468..10424)
                     /locus_tag="N7S94_RS25710"
                     /old_locus_tag="N7S94_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_039899550.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     ARG             6205..7826
                     /name="mcr-4.3"
                     /gene="mcr-4.3"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-4.3"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9474..10424)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.376"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1139..2232
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.726"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggcaaatcg ctgaatatgg ggtccgcttg gaaaacggaa attttcctac gttaagcgtt
       61 ttgagcttaa cgagtgattt cgttccattg agcgcgaacc cctgaccgta agggatgtac
      121 ggaacgatct gactgtccgt taacagtttt gcttactttc gtacatgtta ttggcatggt
      181 gtacgcaatc gttcggtttc ggacacagaa acgctgtagc cccgcgaacc gagtgatatc
      241 caatagccga ttggttaaaa agtagtctta gcgtaggtta ttttcccttt tccaagcgga
      301 ccccaatatt cctttcgtct ccgaccatca gacacctgag tcgctgtctt tttcgtgaca
      361 ttcagttcgc tacgctcacg gctctggcag ggaatggggg taaatgatgc tacaggcgct
      421 tttcatggac tcacacgagg aaatctaccc atgacacaag aaaagcccgt cacgggcttc
      481 tcagggcgtt ttatggcagg tctgctatgc agagctgtca gacttcttgc tgttcagcag
      541 ttcctgacct ctgattttcc agtctgacca cttcggatta tccctcgaca ggtcattcag
      601 actggctaat gcacccagca aggcagcggt atcatcaaca ggcttccccg tctggctgtc
      661 aaccagaccc gccaggataa gcagtccggc tgactggtac agcgcatggt cacgggcttt
      721 acgggcggct ctggcttctg cccgtttttc ggcctgtatc agattcatga cttttgctct
      781 ggcttcctgc gctttttctg ctgcctgttc agccttcatc agcagggaca gcgttttgat
      841 atcctgcgct gttttatccg ctttatccgt cagtatcagc atgagtttct gctgttcatt
      901 cggcgacttt aagccgcgaa tatactgcac tctttctgct gcccattgtt cctgtttttc
      961 gcttttactc atgcccgttt accctctgag ttttaacggg ggaattatgc tcattgatgg
     1021 cagtggaggt caaattcaac tacaatccct gataactctt ttatttatca gggcgcactt
     1081 agacgtttct tcacttcgtt cagaaacggt gcgcgccttc agcgggataa aaacacgatg
     1141 gcctcctatc acctcagcgt aaaaaacggc gggaaaggca gtgcctctcc ccatgccgat
     1201 tatatttcgc gtgagggaaa gtatgcccgt gaaaaagact ccgaccttga gcacaaggag
     1261 tccggcaata tgcctgcctg ggcagcgcat aaaccgacgg agttctggaa agccgctgac
     1321 acgtttgaac gtgccaacgg ctgtacctac cgtgaaatcg agattgccct gccgcgtgag
     1381 ctgaaacccg aacaacgcct tgaactggtc cgcgattttg tccggcagga aatcggcgac
     1441 cgtcatgcgt atcagttcgc cattcataat ccgaaagcgg cgatagccgg cggcgagcag
     1501 ccgcacgcgc atatcatgtt ctctgagcgg atgaatgacg gtatcgaccg tgacccgcag
     1561 cagtatttta agcgagctaa cagcaaagcc cctgagcgcg gcggtgcgaa aaaggcacgt
     1621 ttcggggaaa cgccgacaga acgcaaagag cacctcgtcg cccagcggga acgctgggct
     1681 gacctacaga ataagcacct tgaacggtat cagcataccg accgcgttga tgcacgctca
     1741 ctcaaagctc agggcattga gcgtgagcct gagcggcatc ttggcgcggg acaggttcag
     1801 cggttcgata ctgcgcagtt acaggccatc cttgagcgca gggacgctga aaggcaggcg
     1861 gagcagtctc gtgatgaacg tgacagtgtg attgacgtga ccacctcgct acgggaagcc
     1921 atcagcgagc gggataacct gacgataaaa caagagcaga agcctgaacc tgcgcaggaa
     1981 gacacttccg gcagggtttt cgattttgag aaagagccgg agaagctgaa cgcactggtc
     2041 agtgatgcgc tgaaggacgt acaggacgaa atcgaccttc agtcccttgt gaacgagaca
     2101 atggcggagt ttcaggggat acatcaggaa atggagcgac tggcagaaaa acagcgtcag
     2161 caggagaaag aacagcagag actcgccgaa cagacacgac agaagccgga taaaggctgg
     2221 tcattttcac gatagacagg atttttcatg agcgacgaaa ttaaacaaat cgctgccctc
     2281 atcgggcatc atcaggcgct ggaaaagcgt gttacctctc tgactgagca gtttcaggac
     2341 gcctcagcac aattacagca gcagagtgac acgctgagca ggctcattcg tgagctggac
     2401 agcgcgtccg gtaatatgac tgaaaccgtc aggcaatctg ttcatgccgc gctcattcag
     2461 gttgagaaag agctgaaaca agccggatta gcacagcaga aaccggcgac agaagcgctt
     2521 aatcaggcta ccgatacggc gaaggcgatg atccatgaaa tgcgacgcga gatgtcgcgc
     2581 tatacgtgga aatccgcgat ttatctcgtg ctgacgatat ttttcgtcat ggctgcctgc
     2641 gtgacggcat ttacgtggtt tatgaatgac gggtacagcc agattgctga aatgcaacgg
     2701 atggaagcgg tctggcagaa gaaagccccg ctggctgata tatcgcgttg tgacggaaag
     2761 ccctgcgtta aggtggacac ccgagctacg tatggtgata aagagaatac ctggatgatt
     2821 atcaaaaaat aacataacgg cgcacattgc gccgttattg ttgtcgtgaa ttagggggtc
     2881 agtttggata tagagaatta ttgtacggta agcctgtttt ttgaacagac ttacctatag
     2941 caatagggtg tactcccgcg ctatacagcg ttttcagggg attttgtctt aatcggataa
     3001 cctaaatgcg atacagcaag agcgacaacc taaaagcgat agccactgac gataaatcaa
     3061 tcacttacgt atccgaattg gttaaataat gtgcttaacg tacaaaaatt tccgatctcc
     3121 aaactgaccc cttgatggcc gttttgttct tgcgtggatg ctgtttcaag gttcttacct
     3181 tgccggggcg ctcggcgatc agccagtcca catccacctc ggccagctcc tcgcgctgtg
     3241 gcgccccttg gtagccggca tcggctgaga caaattgctc ctctccatgc agcagattac
     3301 ccagctgatt gaggtcatgc tcgttggccg cggtggtgac caggctgtgg gtcaggccac
     3361 tcttggcatc gacaccaatg tgggccttca tgccaaagtg ccactgattg cctttcttgg
     3421 tctgatgcat ctccggatcg cgttgctgct ctttgttctt ggtcgagctg ggtgcctcaa
     3481 tgatggtggc atcgaccaag gtgccttgag tcatcatgac gcctgcttcg gccagccagc
     3541 gattgatggt cttgaacaat tggcgggcca gttgatgctg ctccagcagg tggcggaaat
     3601 tcatgatggt ggtgcggtcc ggcaaggcgc tatccaggga taaccgggca aacagacgca
     3661 tggaggcgat ttcgtacaga gcatcttcca tcgcgccatc gctcaggttg taccaatgct
     3721 gcatgcagtg aatgcgtagc atggtctcca gcggataggg ccgtcggcca ttgcccgcct
     3781 tgggataaaa cggctcgatg acagcggtca tattctgcca tggcagaatc tgctccatgc
     3841 gggagaggaa aatctctttt cgggtctgac ggcgcttagt gctgaattca ctatcggcga
     3901 aggtgagttg atggctcatg atgtccctct gggatgcgct ccggatgaac atgatgatct
     3961 catatcagga acttgttcgc accttccctg tctatcgcac atttttgcaa atcccgtcga
     4021 ttatttattt ccatatgaaa ctgaaatccg tagcatagtt tgacgaaaag aacaggccat
     4081 caggatgaaa atggcccggg taacaagcca ccacctgaaa cggatgtcag cctctaggag
     4141 ctgtaaccta agggcggtag cgtgcctgcg tcaggccact tgttctgaaa aaccgagcgt
     4201 cccgcccccc aaccattaca gatcagacaa agggctacgt accccattgg cgccgttttg
     4261 cagcacatgg gtataaattt gtgtggttcg aacatcagaa tggcccagtt gctcctgcac
     4321 ggttcgaata tccgcccccc gcgccagcaa atgggtggca aaggaatggc gcaaggtatg
     4381 acaggtgacc ggttttccca gctgcaactg acgcgccgtg gcacgaaccg ctttttgcag
     4441 ggtggttgga tccagatgat gtcgacgcaa ggccccatcg acaggatcgc gactcagatg
     4501 ggatgccgga aacaggtagt gccaacccag ctgccccagc aaaacacatt tcgcgtgtgg
     4561 tggctggtgg ctatataaca aagaacttgt ccgctgactt ataggggttg taagccggaa
     4621 ccgccgaaat ttccgttagc cagaggggca actcgcgtct gaacctcagt cccgcccatc
     4681 ttggctcatc acccagcttg cgtagcacag caccctttag atcagcggac atggcgcaga
     4741 gccgtaggaa gagctgcaag ccgtaactac acgagagatt ggtcaagcat agccaaccca
     4801 ccccatgccg attgcaatgc ctatcccgat agccatcatc ccaggcaggc agcgcaacag
     4861 gaagtcgcgc cctgcgaccc aggcgaggag gccagttttg aagcacaggt tagagagaat
     4921 cgccaggccg atcgcgttag cggctacctc ggcgcccagt ttcccattcc caaacaggcg
     4981 cagggtcgac aatgcgatgg catcaagttc ggtcagcccg gacacggcgg acgccaggta
     5041 gggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag gccttacatt
     5101 tcaaaaactc tgcttaccag gcgcatttcg cccaggggat caccataata aaatgctgag
     5161 gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc gtaagccgtc
     5221 ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc atgatcgcat
     5281 tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct
     5341 tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt gttgatgaat
     5401 cgcgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg gtatgcagct
     5461 ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct gtcgacggcc
     5521 cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat gtgccacggg
     5581 caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc aggcgcataa
     5641 cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc agccagcatc
     5701 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     5761 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     5821 ggatgataag tttatcacca ccgactattt gcaacagtgc ctgtaaaact cacggatacc
     5881 ttaaaacagg ctatttatga taaaagagct gagggggcca ccattgggca gcttgccaag
     5941 gagtatcacc ttggagaagc ctctatttat cgggcactga attctgtgaa gagcacacaa
     6001 gaaattacaa gtaaacttat aagtaattga agtttattta cttaattgtt tacatgtaac
     6061 ttttaggtga attgggctca attcacctaa aacggtctta tctgttaagt ttgttggtga
     6121 ctcattaaaa taacctttta atcataaggt tattttaatg aggtcaagct agtattgttt
     6181 tttttagcaa aaaaggggtt tttgtgattt ctagatttaa gacgttatcg gttaaccaat
     6241 tcactttcat cactgcgttg ttttatgttg ccattttcaa tctaccgctc tttggtatag
     6301 tgcgaaaagg aattgaaaaa caaccagaag ttgatcccct tttcatcgca tctatgccgc
     6361 tatttttaac atttgcgctg agttttttgt tttcaatttt taccgtcaaa tacctgctga
     6421 agcccttttt tatcgtattg acgttacttt cctcaagtgt attttttgca gcctatcaat
     6481 acaatgtcgt gtttgactac ggcatgatag aaaacacgtt tcaaacacat cctgctgaag
     6541 cattgatgta tgtaaatctt gcatcaatta ccaatctact gctgactggg ctattaccgt
     6601 catatcttat ttataaggcc gatattcatt atcagccctt ttttaaggag ttattgcata
     6661 aattagcctt tatgctgcta atgttcgttg gcattgggat agtcgccttt ttttactatc
     6721 aagattatgc tgcatttggt cgaaacaaca gtgagttaag gcgttacatt gtccctacct
     6781 attttgtcag tagtgcatct aaatatctca atgagcacta tttgcagacg cccatggaat
     6841 accaacaact tggcctagat gcgaagaatg ccagtcgtaa cccgaacact aaacctaact
     6901 tattagtgtt tgttgtgggt gaaactgcgc gctcaatgag ctatcaatat tatggatata
     6961 acaagccaac caatgctcat acccaaaatc aggggctgat tgcgtttaac gatactagct
     7021 catgcggcac ggccacggcg gtgtctctac cctgtatgtt ttcacgaatg gggcgggcag
     7081 actatgatcc tcgccgtgct aatgctcaag acacagtgat tgatgtgtta agtcatagtg
     7141 gtataaaagt acagtggttt gataatgatt ctggctgtaa aggtgtgtgt gatcaggttg
     7201 aaaatctcac gatagatttg aagagtgatc cgaagctgtg ttctggccaa tattgttttg
     7261 accaagtatt gctcaacaaa ttagataaaa ttctggcagt agcaccaagt caagatacag
     7321 taattttttt gcatatcatt ggtagtcatg gaccaactta ttatcttaga tacccgccag
     7381 agcatcgtaa atttataccg gattgtccgc gcagtgatat tcaaaattgc agtcaagaag
     7441 aactgattaa cacctacgac aacactattc tatatacgga ttttattctc agtgaagtgg
     7501 tgaataaatt aaaaggtaag caggatatgt tcgatactgc aatgctgtat ctctctgacc
     7561 atggtgagtc tttgggtgaa aagggcatgt atttacatgg tgcgccctat agtattgcac
     7621 cgaaagaaca aactagcgta ccaatgctgg cttgggtatc taatgacttt agccaagata
     7681 atcagttgaa catgacttgt gttgcacagc gagcagaaca gggcggcttt tcccacgaca
     7741 atttgttcga cagtttgcta ggacttatga atgtaaaaac caccgtctat cagagccaac
     7801 tcgatatttt tgcaccttgc aggtattaga tatggctgta aatgttttat cttaggggtg
     7861 acatattcac cccttaaatc ttggactaac aatgtcctct attgttacat ttagctctca
     7921 atcttccaca atgagttatc acggtctttt agacttaaca tcaatttgcc gttgtgaaac
     7981 tagaacaatg gcttacaagc tgtaaatgct aactttacat ttttggcgcg ttgggcaaaa
     8041 ttagcctatt tatatccctt aggattatct catatgtgtg aaatgatggt tttttgcaaa
     8101 aaaactcctt gttcaacata agcgatcttg cacgcatcaa aaggctgctt aaagcggcct
     8161 tgaaaattac aacgtgatct gagggtgctt aacaccgcat gctcttgcgt atcgagccag
     8221 tgtttcaatg ctagccttaa tcacattaga ttccatgcga gaaacggtag gtggagtaac
     8281 acccatgcgt tctgccacct gggctcttgt taatcctgcg tgatgacgcc attcagcgag
     8341 catagcttga agtctttcct tgcgctcctc tgcttcatag gctgcttgaa cctcaggatt
     8401 agacatcaat tcattcctaa catgctccca agcgatacct ttaactttac tcatttagca
     8461 tctccttaag acgttcaaag gccatagata tttcacccga tggggttttc tgagtctttt
     8521 taataaagac tctaagtaga tagatattcc tgcctttctg ataaacataa agacctctgg
     8581 caatatccgt ccccatcgtc cttaactcaa ataatccatc ccggagtggt ttgctatctg
     8641 gctctcgtaa tgcagtagcg ttggttttaa gtttatcaat caggcgaatc atcttcgctc
     8701 tgactactgg cggtaagcca gccagctcat ttggtacatc atcgtgcaat atgacgttaa
     8761 acactggttc gccctccatt gcgaaatatt agcataaaaa gcaatttgcg ttatagggta
     8821 attttatggt ttttctgctg tatttaacat aagcgatctt gcgcgcaccg cccgaacacc
     8881 actcgccaca aaaacccgcc ggaacgtcca aaagtacggg ttttgctgcc cgcaaacggg
     8941 ctgttctggt gttgctagtt tgttatcaga atcgcagatc cggcttcagc cggtttgccg
     9001 gctgaaagcg ctatttcttc cagaattgcc atgatttttt caccacatga ggtgtcactg
     9061 gttccgatgt attcggcagt ttagactcga ttagcagcaa agcatgtttg agatcctcat
     9121 tgcgtttgtc tttttcatcg agtagtagct ctttctcacg cagcaatgcc tttacgagtt
     9181 ctatttcagc ttttaaggca tagttttcat gtagcaaatt ctggtcaggt aactgcacaa
     9241 aatcgcctgc caaatgctgc acagtactgc ctgattgttc actgccaacc tgctgaccag
     9301 taaatgatcc gaaaacacgt agaagctctg atgtgtctag ttttgaagca ccaccgctat
     9361 ctgtgcactt ggtcagtttt ccctgcttaa tgtatgactg tactgttgtg cggctttttc
     9421 cagttagtct ggctgcttcg cttattgata ctaaagccat agatacctca gttccgtccg
     9481 tacttagcta aaattttctc tagcgtggct cgttgttttt gagtcagcca tgtaaatgag
     9541 ccgttgagat catacttact ctgcatgtca ctcaggaatt tagcttcaaa tccagtaagt
     9601 tgtattttcg cagttaaagc atcatgtaat gttgttttta gcccctcatg cagacgattt
     9661 tcaggcgtta aacggattga gttatcttct ttttttgata ttggttcttt tgctagctca
     9721 gtcacaaggt caatttgttt atccagttca acttggaaga tcagcgtatc agcaggacga
     9781 ccgcgcttat caatagatag ttttatattg ctataggtat ttaaatcttt tgaaattggt
     9841 tttaaaaccc aatgatttag taatttatat tctgggtagc ttttttctaa cattagcatg
     9901 aatttaaact catcaatgct tatcgaaata ttggctttat gtgtttttct ttgcgttagt
     9961 tcttttagca gccattcata aatgcgcatt gagtatttat tttcaaagga cttaacatgc
    10021 tcgaggttgt atttaatgaa tctctttagg tgaaatagca atgggattat atatttacta
    10081 aaaactaaag ttacctcttg tgtttcccta ttgaactctg catattcagc ccaattaaaa
    10141 acacgcatag atacagcacc tgttggatat attaattcaa tagaccgctt aaataactct
    10201 cgtgttgatg tatttaatcg cccccacgca ttctcataac ttatccccat aaatgaagaa
    10261 tattcggagc atgaaaattt aaccgttcgt tcagtttcat ctggtgtttc taatgtaggg
    10321 tttagctttg caacacaaaa caggattagt ttcgtttcgt gctctgttag gtcataacga
    10381 ctaaccgcaa gctcgtttgc tttgaatact accaattcag ccatgactaa aaccattggt
    10441 tgtgatttta aaacaacata acccttccgc ttggttatgt caatttcatt gtcataacct
    10501 tttatgtggt tgtgtggatt acctaatttt cacttaacct tttgaagggg gatcctaatt
    10561 tttgcttaac ctttcctaat tttcacttaa cctttcatgt gttttttact tataaaacaa
    10621 aatattaaaa tcactagaat aaagaaagaa taaaaaagat aaaaaagaat agatcccagc
    10681 tttgtggata actaaccgcc agtgccagtt gagcgatatg ataaaaggat gtcgcaaaca
    10741 gcgtttgctc ctccaaaaaa cataccttca aacacgaaag aatgaggtag caccctcgca
    10801 ag
//
