LOCUS       NZ_CP104801             5250 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Klebsiella pneumoniae strain GX34 plasmid p5, complete sequence.
ACCESSION   NZ_CP104801
VERSION     NZ_CP104801.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30914361
            Assembly: GCF_026821715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Zhang,F.
  TITLE     a NDM-4 and OXA-181 co-producing K51-ST16 carbapenem-resistant
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2022) Laboratory of Clinical Microbiology and
            Infectious Diseases, China-Japan Friendship Hospital, No. 2 East
            Yinghua Street, Beijing 100029, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 03:55:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,861
            CDSs (total)                      :: 5,731
            Genes (coding)                    :: 5,558
            CDSs (with protein)               :: 5,558
            Genes (RNA)                       :: 130
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 84 of 173
            Pseudo Genes (incomplete)         :: 89 of 173
            Pseudo Genes (internal stop)      :: 14 of 173
            Pseudo Genes (multiple problems)  :: 13 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104801.1.
            Bacteria and source DNA available from human.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GX34"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China:Guangxi"
                     /collection_date="2020-06"
     gene            complement(join(5132..5250,1..409))
                     /locus_tag="N7T97_RS29205"
                     /old_locus_tag="N7T97_29210"
     CDS             complement(join(5132..5250,1..409))
                     /locus_tag="N7T97_RS29205"
                     /old_locus_tag="N7T97_29210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_268857212.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRLKQIYLLEMRATPGKQSSLVGIIKEV
                     LKKYHSSLIIHT"
                     /name="AAA family ATPase"
     gene            complement(1042..1254)
                     /locus_tag="N7T97_RS29210"
                     /old_locus_tag="N7T97_29215"
     CDS             complement(1042..1254)
                     /locus_tag="N7T97_RS29210"
                     /old_locus_tag="N7T97_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1258..1743)
                     /locus_tag="N7T97_RS29215"
                     /old_locus_tag="N7T97_29220"
     CDS             complement(1258..1743)
                     /locus_tag="N7T97_RS29215"
                     /old_locus_tag="N7T97_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1781..2458)
                     /locus_tag="N7T97_RS29345"
                     /pseudo=""
     CDS             complement(<1781..2458)
                     /locus_tag="N7T97_RS29345"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2421..2741)
                     /locus_tag="N7T97_RS29225"
                     /old_locus_tag="N7T97_29230"
     CDS             complement(2421..2741)
                     /locus_tag="N7T97_RS29225"
                     /old_locus_tag="N7T97_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3378..3539
                     /locus_tag="N7T97_RS29230"
                     /old_locus_tag="N7T97_29235"
     CDS             3378..3539
                     /locus_tag="N7T97_RS29230"
                     /old_locus_tag="N7T97_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(3692..4312)
                     /locus_tag="N7T97_RS29235"
                     /old_locus_tag="N7T97_29240"
     CDS             complement(3692..4312)
                     /locus_tag="N7T97_RS29235"
                     /old_locus_tag="N7T97_29240"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(4305..4973)
                     /locus_tag="N7T97_RS29240"
                     /old_locus_tag="N7T97_29245"
     CDS             complement(4305..4973)
                     /locus_tag="N7T97_RS29240"
                     /old_locus_tag="N7T97_29245"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_227539852.1"
                     /translation="MVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFS
                     HPTDEGGSVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVK
                     LFNDPDVNKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKT
                     TTPYAAWYDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE
                     "
                     /name="ATP-binding protein"
     Typing          complement(2856..3028)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          2975..3488
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(927..2431)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagatggata atagtctaat gattcaaata aaatccgatt tttaataaaa gcaacttcat
       61 aactgaaccg attcccctca acagaataaa attcagcctc aaactttaca ggagcctcat
      121 tcgtagccga agaaagagca tatggctcat agcagggaat ccgtttacct tctttcaaat
      181 caccgctttc tgttgcgata aattttatag cttcaaaagc catgagaacg ttagatttac
      241 cagacgcatt agcaccataa atcccagcac tcatgagcgt tccaacattc attccatcat
      301 ttttatagat atgagttgtt aaatggttac tagaaccgga tgcgctcaag tttaatgtcg
      361 atttttcctt aaccgaccta aaattttcaa tagtcaaata aacaaacatg catacctcct
      421 aacggggatt tttcgccaaa aaagcgaaaa atcaatcata ggaaagatat cctatatgtt
      481 ttaattttgg aacaacatct tccttaaaaa acgttgtgct atttataatt acacgctatt
      541 taccataaca tctgttgccc gcaccgctcc ggacaggttc gccacaaacg gggcggcagt
      601 caggtaaaac cggcaacaaa ccacccgata caccgccccg ttcgtgacag ataaatttta
      661 cccggctgca tggtcatgca gccagatgca tacggtctga tttcaggcca gaatccggat
      721 gaacgcctta aaaacaggaa aatgcttctc cagccagtcc cagatctcca gcgccatgta
      781 caggcaattc agcaccgtct caatcacgtt aaacagtctc ataatccctc cgttgtatac
      841 gctgtgatgg aaacatcacg accgtattta gcgggaaatt tatgagccct tcttcttggg
      901 gtttgtcact ggatgcgcgc agtcagccca gcgtcggacc gtcatagctc cgctgccggg
      961 aacgggtttc cgccagctct ttttcatgct gtttctgctc gtatgcccgt tcggcggcca
     1021 ccgccatttc atgccgctgc actactggct caaacgctgt aactgcctgc tcaagttcgc
     1081 cacctgctga ctcaagcgcg tgacgcgctc gttcagcgcc gcgttctccc gttgcataag
     1141 accagacatc ttctgccatt ccgcgaaggc gctctcccac tcgtccagcc tttgcgagta
     1201 gtcctgatgt agctgctcta atgcggtcag caactgtttt tccagttccg tcatgggcta
     1261 tttcctctcc agtaccatcc agttcccttc ctccccgtaa gctcccgctt tcgggttcac
     1321 tttgacgcag gacagtttct tctccccgca gagcgacagc tgcacgccgt ggttcttctc
     1381 cgacagcatg gcctgcgtcc gctcctgcgc ccggatagtc tggtaattcc cggtgatctg
     1441 ctgcccctgc caccacagaa tgctcccgct cgtggcgatc agcagtaccg agaccagggt
     1501 gatggtcagc cacgtccggc cgaccatacg cagcacgctc agccggttcg actgcatcgc
     1561 cgcggtcata ccctgctcgt gggcgctgat ggcgtcgctg attctcttcg cgcttgcgct
     1621 cagttccgct tttacagact gctcgtgctc gctgaatgcg gctttcagca tctcgccggt
     1681 actctgctgc tgcgctttcg attgctgctc caagtccttc gccagcgcta aaagactgct
     1741 cataaatggc tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatac
     1801 tgttttttgt ggtgcggacc acctcaaaac ctgccgctgt gagcgcccct gtgacgtcct
     1861 cgcgcgtttt gagctccccg gatgaggcta aggccagcaa cccacgtgta atcgcctgag
     1921 cggcttccag cttcgtttcc ggcagtgcgg acggcgtgac cagtaaccgt cggttctccg
     1981 gggcgtttgg atcatgcagc cccagcctgc cgttcaccac ggtctgccag gcatcgatgc
     2041 gcggccggtc ggcgcgatcg tagtacggct gcaggcgttt cccggtcagc agttcagtgt
     2101 tggggatcag gaaattcagc tccagccgcc ccttgtcggt gtgctccacc cacagaatgc
     2161 tgtactggtc tttatcgagc ccgggcatca gaacccgctc aaagctggcc atgatctgct
     2221 cccgctgccc gggcggcagt tcagcctccg cgaacgacag cacgccggag gtgtactttt
     2281 tcgcatacgg cgaggcatcg ataagctccc ggacttcctc cggttttccc tgcagcacgg
     2341 tcgccccctc gcgctgccgg tcttttccca gcaggtaatc caccggaccg gcaccaccac
     2401 cgcgcccccg ggggtgaaat ttaacgatca tcatcccccc ctttttccag caccgcatgc
     2461 cgcagccgct ccagtcccgc gtcgattgcc atcagtgcgg ccacaatctg cacccggtca
     2521 tgaccggcac caccggcatt cactttccgg gcgatctggt tcaggttgtt ccccataccg
     2581 gcaagctggc gcagcagcgc cggcgatatc gacggcagtc tgccggcacg cgccggcttc
     2641 tcatcgaggc aggtctgacg catccacgcc gccagctgct tgccgtcgca ccgctcgagc
     2701 agccgccggt gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg tttgtctgcc
     2761 agagtagcct cctgataaca ccgggacggg cgggaaatgt ttcccggtcg gcacggtgtt
     2821 ggacaagccg caggcgcgtc agtcgctttt agcgggtttc ggggcgcagc cctgaaccag
     2881 tcacgtagcg ctagcggagt gtatactggc ttattatgtt ggcatgagtg cggtaatcag
     2941 gaaagtgctt catgtgacag aggaaaatgc agctgcgggc gtaaggcaga aaatggaagt
     3001 tcagaggata tgacgcctcc tcgctcactc agtcgctgcc gctcctgccg ttcggctgcg
     3061 gcgggcgtta cctgcacacg gagaagagcg aaggcatatg gcatgacaaa cggggcagaa
     3121 aaaggatgcg ccgctgccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     3181 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3241 ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     3301 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     3361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttctccg
     3421 gtaactatca tcttgagtcc aacccggaaa gacacggttc atcgccactg gcagcagcca
     3481 ttggtaacgg attagaggaa gtgagtcttg aagttctgcg ccagttaagg ctaaactgaa
     3541 aggacaattt ttggtgactg cgctccctct aagccagtta cccggtacat aagttggtag
     3601 ctcatgtacc ttccaaaaac caccgttggt agcggtggtt tttatttttc atagattaca
     3661 gcctgtaata agccatggtc aatggattac ttcaaaagtc gtcaatcgcc ttaagtagct
     3721 catggaatga tgtataacat ggaaatagat aaggttcgtg cgtgttttta taattagttt
     3781 tagcgtaaat atctagcttt cttgcgttat ctttcgcagt accaatcctc tctcttatag
     3841 caaggtaagt tagtttacta cctttatcat agttagttat taattttttt agcggggagc
     3901 tagacatcaa attatcatac gacgaaaaat ctttggttgt gaagccgaaa tgcaacaata
     3961 accataattc aaaacaaaca caggatattg ctatatttat cccatgccta ttagctttat
     4021 tccatgcctt ttgatgacat gaaatagacg aaggcgtcag atgctcttga tcataaacaa
     4081 cccaaaagac gtcaccttct gcggaagatg atgaattctt tttctttatc gcctcatcca
     4141 ccaactgctc aggtgtattt ttccttgttt taggtatttt aaatacactt aaagttcttt
     4201 tagatctgtc tgaaatataa gcctcaaggt ataatggttc ggttttctca ccctcacagt
     4261 atatatacat tacttgtggg actttcttat tatttccatg ttttttactc atcagaaaga
     4321 tccccgtcaa actcttcaaa aaaaccatca gacaattctt tgacgtcaat atccggcatt
     4381 accaaggata tatcaccaga tataaatgag gctaccttta agtagtttat gtcgggaacc
     4441 ccaccaaacc taccctcatc ataccaagct gcgtaaggtg ttgttgtttt tatctttttc
     4501 ttatcaaaat catctaagct ataaagagac gacgcacctt tatttttttc acaaaaccat
     4561 atctgatctc ttctcatttt ctctggagcc attaactgca tattatgggt tgagaataca
     4621 agttgtgagc cttttttatt gacatcagga tcattaaata acttaacaat caagtcagca
     4681 atatgcgggt gcaagctatt atctaactca tcaacaatta caaccatact atcattaaac
     4741 gcagatctaa ttagaggtat gatttcaaat aatttttggg tgccttcaga ctctctcccc
     4801 atagggaaag aaacactccc cccttcatca gtaggatgag agaacatata acgatattta
     4861 taatcttctt caattatctc ctttatttct tgagggattt cctcatccat ttttattggt
     4921 atttccgcct caacctcccg tacagatata ccggaaacac cagtgtcaac catgcataaa
     4981 accttggccg tttcatttac gacgtcctcc ctctccccaa aagaagatat tcttatcttt
     5041 tcatttaaac ctatatgtct aattcccttt ctaaaaaagt tataagcttc tttaattatg
     5101 tctggtgatg cggcattatt acctgctttc gctaagtatg aattattagg gaagaatggt
     5161 atttttttaa tacctccttt ataatgccca ccaaacttga ttgtttccca ggtgtcgctc
     5221 tcatctctag taaataaatt tgctttaacc
//
