LOCUS       NZ_CP104688             1240 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2022CK-00450 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP104688
VERSION     NZ_CP104688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30655617
            Assembly: GCF_025398755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00450
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:52:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,192
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,823
            CDSs (with protein)               :: 4,823
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 58 of 252
            Pseudo Genes (incomplete)         :: 183 of 252
            Pseudo Genes (internal stop)      :: 42 of 252
            Pseudo Genes (multiple problems)  :: 34 of 252
            Pseudo Genes (short protein)      :: 4 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104688.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00450"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-20"
     gene            142..651
                     /locus_tag="N5922_RS25820"
                     /old_locus_tag="N5922_25810"
     CDS             142..651
                     /locus_tag="N5922_RS25820"
                     /old_locus_tag="N5922_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_261588849.1"
                     /translation="MIVASGHQAKLRQKEIQEIDKKASKEHEEAKKNSNFTQTYPKGWQ
                     RIRELVRDKQGAAAIGLYSFFAEHIDPTCGAVVCDQQFLANQMGVSTRTIRRWLDYLEE
                     KGALVRIPVAGKVCAYALDPHEVWKGYNTSKDYSAFVTKTLVNKDGEIKRRIMAMFSGE
                     ESGQEQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 agttttggtt ttttctgata ggacacagct ggtgtccgca cgtgaggaca cagctggtgt
       61 ccgcatgtgg ctggacatag gacaccacaa gtcctatgat gggacaaaaa tgtcctatca
      121 aaaagacacc gagggataaa gatgatcgta gcttcaggac accaagcaaa gctaaggcag
      181 aaagaaatac aggaaatcga caagaaggca tctaaagagc acgaggaggc aaagaagaac
      241 agtaacttca ctcagacata cccaaagggc tggcaacgca ttagagagct tgtgagggac
      301 aaacagggtg ctgccgctat aggtctttac tcatttttcg ctgaacacat agatcctaca
      361 tgcggtgcag tggtctgcga tcagcagttt ttagctaatc agatgggtgt ttctactcga
      421 acaattcgtc gttggttaga ttatttggag gagaaaggtg ctttggtaag aataccagtt
      481 gctggaaagg tttgtgctta cgctttagat cctcacgaag tctggaaggg atataacaca
      541 tcaaaagact actcagcctt tgttaccaag acattagtga acaaggatgg agaaataaaa
      601 cgccggataa tggctatgtt ttccggagag gagagcggtc aagagcagta gcgaagcgac
      661 tcttgacggc tcggaacgac ccccaccctc agaaaggggg atggttcgcc acagcgagcc
      721 atccccataa taagccgccc tttaagcagt agatcccata aataaataaa acagcacatc
      781 acttttgtaa tgtgcggcat tcctttggga ctaaaagaag ttagccagga atgtaataca
      841 gaaactttac cgacaaggag gtcatcatga tcacgtcaac aatcatcaca atcatcggct
      901 taattgggag tctcataacg atttaccgag agatataccc gaaggataag cactaattag
      961 ccagtataca ctccgctagc ggagtgactg gttcagggct tcgccccgaa acccctgaaa
     1021 accgtcaatc atggtgatgg ctcactacgc gagccatcac cagatacaag gttaaacggg
     1081 cattttgatc gtcaaggtca agccgctaac gcggtcgcct atggcgaacc ctgaccctca
     1141 aaatgcccga ccggagggag ggagctacct tgaactttgg tgttgtctgt tggttttcat
     1201 agaagagaat agagataaat aataagttat tgatttttat
//
