LOCUS       NZ_CP104686             3223 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2022CK-00450 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP104686
VERSION     NZ_CP104686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30655617
            Assembly: GCF_025398755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00450
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:52:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,192
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,823
            CDSs (with protein)               :: 4,823
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 58 of 252
            Pseudo Genes (incomplete)         :: 183 of 252
            Pseudo Genes (internal stop)      :: 42 of 252
            Pseudo Genes (multiple problems)  :: 34 of 252
            Pseudo Genes (short protein)      :: 4 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00450"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-20"
     gene            595..765
                     /locus_tag="N5922_RS25775"
                     /old_locus_tag="N5922_25765"
     CDS             595..765
                     /locus_tag="N5922_RS25775"
                     /old_locus_tag="N5922_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_261588848.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGKWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            complement(974..1306)
                     /locus_tag="N5922_RS25780"
                     /old_locus_tag="N5922_25770"
     CDS             complement(974..1306)
                     /locus_tag="N5922_RS25780"
                     /old_locus_tag="N5922_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1474..1689)
                     /locus_tag="N5922_RS25785"
                     /old_locus_tag="N5922_25775"
     CDS             complement(1474..1689)
                     /locus_tag="N5922_RS25785"
                     /old_locus_tag="N5922_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1693..2178)
                     /locus_tag="N5922_RS25790"
                     /old_locus_tag="N5922_25780"
     CDS             complement(1693..2178)
                     /locus_tag="N5922_RS25790"
                     /old_locus_tag="N5922_25780"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2213..2917)
                     /locus_tag="N5922_RS25985"
                     /pseudo=""
     CDS             complement(<2213..2917)
                     /locus_tag="N5922_RS25985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2856..3179)
                     /locus_tag="N5922_RS25800"
                     /old_locus_tag="N5922_25790"
     CDS             complement(2856..3179)
                     /locus_tag="N5922_RS25800"
                     /old_locus_tag="N5922_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(975..1306)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(53..244)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          224..704
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.663"
                     /coverage="93.0"
                     /evalue="8.04e-131"
     Typing          complement(1377..2866)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acggacggga aatcttttcc cggtcggcac ggtgttggac aagccgcagg cgcggcagtg
       61 gttttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
      121 ctggctaaac atgttatgac ggagtggcaa tttatgaaag tgcttcatgt gacaggggaa
      181 aaatgcggct gccgggcgta agtgcaaaat gcaggtggtg atgaaatgac gcctcctcgc
      241 tcactcggtc gctacgctcc tgccgtgaga ctgcggcggg cgttaccagc tcacagatta
      301 cgggataagc aaatgccgct cgaagcagga aaggccaaga aaaggatgcg gagttgccgt
      361 ttttccatag gctccgcccc cctgacgagt atcacgaaaa tgacgctcaa atcagcggtg
      421 gcgaaacccg atgcggctta aagataccag gcgtttcccc ctggtggctc cctcgtgctc
      481 ccccgcattc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcggtttctc
      541 attccacgcc tgaaactcgg ttccgggtag gcagttcgct ccaggatgga ctgtatgcac
      601 gaaccccccg ttcagtccga ctaccacgcc cgttccggta actatcaact tgagtccaac
      661 ccggaaagac acgacaaatc gccagtggcg gtagccattg gtaagtgggt tcacggagca
      721 gagattgtag agaatgccta cagagttctt gaagtgaaag cctgagtgcg gctacactga
      781 aaggacagtt taggtatctg cgctccgtga aagccagtta ccatcggaac aaagagttgg
      841 tagctctgaa ttccggcaaa cgaaccaccg ttggtagcgg tggttttttt gtttacaggg
      901 aaaaagatta cgcgcagaaa gaaaggatct caaggaaatc cttttgcttt ggccaatatg
      961 cttcggtaaa aagttaatgc gcagaagatt ttgaaaaaat attaataacc aaaactccaa
     1021 cgcaaataag gaacattcct gcaattgcgg gccaatcaag tttttgaccg ctaaccaacc
     1081 aaccagctag gcttattagt acaattcccg caccagacca aattgcgtaa gcaataccgg
     1141 taggtatatt ttccaatgtt tgggaaagca gataaaaggc agcgcaataa cttattgcag
     1201 ttcctacaga cggccacagg cgtgtaaatc cttctgaata tttcataaag atggtgccta
     1261 tgacttcagc cacaatagcc cctcccagga aaatatatgt attcataaaa atacctcatg
     1321 ttgaaaaatt taaatatctt tacataacaa ctaattaata gaacccggct tatcatagcg
     1381 aaggaccatg atgctgccac tgcgatcgtt ccagctccag cgctttctgg tgctgcaccg
     1441 cccgctcctg ctgccggtga gtttctatcg cgttcagcct ttcgacaacc ggcgcagctg
     1501 ctcgctcaaa ctctgcacct gcctgctcaa gaccgtgacg cgctcgttca gcgccgcgtt
     1561 ctcccgttga ataagaccag acatcgtccg ccattccgcg aaggcgttct cccactcgtc
     1621 cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gtttttccag
     1681 ctccgtcatg tgctatttcc ccgccagtat catccagctc gagtcctctc cgaactgtcc
     1741 cgcttccggg ttcaccctca cgcagcggcg tctctgctcg ccgcaggtcg agagctgtac
     1801 gccgctgttc ctctctgaca gcatggcctg cgtgctcttc tgctcccgga tggtcgtata
     1861 gttctcgagt atctgctgcc cctgccacca cagaatgccc gcgctcgacg ctatcagcag
     1921 cgcggagacc agaacgatgg tcagccacgt ctggctgacc atgcgcagca tccctttcgt
     1981 gcgctggctc atggctgagg acagcttccg gtcgtggtcg aggatggcgg cgctgattct
     2041 cttctcgctt tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt
     2101 cagcatctcg ccggtagtct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag
     2161 cgttaaaaga ctgttcatag atggctccct tcagtcggat gtttcgcccc ccgtccgggt
     2221 cggcaatgct gatgctgctt tttgtggtgc gcaccacctc aaaacctgcg ctttccagcg
     2281 cctcagtgac gtcctgacgc gttttcagct ccccggatga ggcaagggca agcaggccgc
     2341 tcgtaatcgc ctgggcggct tcctgcttcg cttccggcag cgcggaaggc gagaccagca
     2401 cccgccggtt ctccggcgcg ttcgggtcat gcagccccag cctgccgttc actatggtct
     2461 gccaggcatc gatgcgcgga cggtcggcgc ggtcgtaata cggctgtagc cgcttgccgg
     2521 tcagaagctc cgtgttcggg atcaggaagt tcagctccag ccgccccttg tcccggtgct
     2581 caacccacag cacgctgtac tggtctttat cgagtccggg catcagaacc cgctcgaaac
     2641 tcgccatcag cttttcgcgc tggccgggcg gtaaatcctg ttcggcaaaa gacagaacgc
     2701 cggaagtgta ctttttggcg tagggcgagg cgtcgataag ctcccggact tcatccggct
     2761 tcccctgcag aacgctggcg ccgtcgcgct ggcgatcttt acccagcaga taatcgaccg
     2821 gcccgccgcc gccgccgcgc ccccgaggat gaaacttaac gatcatcatc agcacccttt
     2881 tccagtacgg catgccgcaa ccgctcgagt ccggcatcga tggccatcag cgcggcgaca
     2941 atctgcacgc ggtcgtgtcc gcttccacca ccggcgttaa cctggcgggc aatctggtta
     3001 aggttgttcc ccatgccggc aagctgacga agcagcgccg gcgagatcga cggaagtttg
     3061 ccggcgcgtg cgggcttctc gtccaggcac gtctgccgca tccaggccgc gagttgctta
     3121 ccctcgcagc gctccagcag acgccggtgc tcgtcctcag tcacccacat ggtgagcatt
     3181 ttgctgcgtt tgtctgccaa tccggcctcc tgataacgcc ggg
//
