LOCUS       NZ_CP104685             3604 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2022CK-00450 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP104685
VERSION     NZ_CP104685.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30655617
            Assembly: GCF_025398755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00450
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 83.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:52:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,192
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,823
            CDSs (with protein)               :: 4,823
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 58 of 252
            Pseudo Genes (incomplete)         :: 183 of 252
            Pseudo Genes (internal stop)      :: 42 of 252
            Pseudo Genes (multiple problems)  :: 34 of 252
            Pseudo Genes (short protein)      :: 4 of 252
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104685.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00450"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-20"
     gene            211..360
                     /locus_tag="N5922_RS25755"
                     /old_locus_tag="N5922_25745"
     CDS             211..360
                     /locus_tag="N5922_RS25755"
                     /old_locus_tag="N5922_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001417847.1"
                     /translation="MPSEYDEQRDANDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            744..1112
                     /locus_tag="N5922_RS25760"
                     /old_locus_tag="N5922_25750"
     CDS             744..1112
                     /locus_tag="N5922_RS25760"
                     /old_locus_tag="N5922_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017880027.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSKNDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     gene            1685..2377
                     /locus_tag="N5922_RS25765"
                     /old_locus_tag="N5922_25755"
     CDS             1685..2377
                     /locus_tag="N5922_RS25765"
                     /old_locus_tag="N5922_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="WP_157703753.1"
                     /translation="MHLLYVDESGTPSDSTLNHFVLSGISVFEKQTHWIEQEMNAIAAR
                     FNADDPYERELHGSPMRGGRDGWKNFSFNDRKQAIKDCLALLTQKHRDIRIFACVVPKG
                     ALDGKDVITESFYQISSRFDMYLSRLHHQGATSRGVAIFDKSSTEKSIQSLARTFKDTG
                     YEYGKLRNFSEVPLFLDSKASRLIQLADLVSYSIFRKFEFNDDEFYKIIENRFDYHNGK
                     VHGLYVAH"
                     /name="DUF3800 domain-containing protein"
     gene            complement(3132..3245)
                     /locus_tag="N5922_RS25770"
                     /old_locus_tag="N5922_25760"
     CDS             complement(3132..3245)
                     /locus_tag="N5922_RS25770"
                     /old_locus_tag="N5922_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517211.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          3..176
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(3137..3604)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.843"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc
       61 cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa
      121 gccagtatat actctgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct
      181 aacaactcct tagctactgg atatcacccc atgccatctg agtatgatga acaacgggat
      241 gcgaatgata cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact
      301 gaagagaacc tgcacgcccc ggtggatttt gggccgcctg tcggcaggga agcgttataa
      361 cttttttcgc tggtcagcgt acgtgtaata cgccggtatt acatcttatt tctttatttt
      421 catgtgatta caggtggttg taatacgttg tgtattacat aaataaacgg gaaattcagt
      481 ttttaccttt taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg tgtatggcca
      541 tttaagggat aatgtaacct gacttttaaa gaatgaatct atctttggca gcgcatgaga
      601 tcgataaaaa gcgttccaca tcaggcggtt catggataca tgaaccggaa aggacaacaa
      661 taagacaatt gtaagacatt ttttgcgttt tactcttatt tttggactat tatgagacaa
      721 atataagaca ttatgggcgg tatatggcga attttaaccc tggtccaaga acagccattg
      781 cagctgtcag ggattccaag aacgatatag aaacctggaa gaaaaatggt atccgtaggg
      841 aagagattgt gactgtgctg aatgaacgtt acggcctgaa tatctcactg gttgcgtttg
      901 ataaggctct ttacagattg agaaaaagta cagatgaacc gtctcttgct gatgtggatt
      961 ctgtgtctgc tgcagaaacg aatttctctg aagccagtgg gcctgaaaat ggtttttttc
     1021 tgtccaagaa tgacttcatg cgagcagaag aagaatctca aagaatggtt gagtcattca
     1081 gagatagcct tggcaggaag aaaggattct gattttgaat tctaagtagt gaaatagtgg
     1141 atcctctact cgaaattgtc ggacacctgc aattacctac cgtgccttac ggcttggtca
     1201 atgccttacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat aagaagaaca
     1261 tcggaaacag gacttacccc ggctgaatgg tgtgaaattc tgcgctatgc acttgcgcgc
     1321 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     1381 gttgcctgtt ttaccggttt cccgtcagaa acgccctgag agcctctcag gcggtgcacg
     1441 caacagatgt tatggtaaat cagaatgacg gtgaagaaac tatctgattg aatttatgtt
     1501 atttgattgc tcatggctat ggtgtgtgta taaattattt gggtaaatat gcacttgcct
     1561 gttatcattg atattgagtc agttcgctgc ccatagcggt gtatgcgatg tttatcgcac
     1621 tggcttgcct aattcataaa aaggccgcgc atttgcgcgg cctttttcgt ttaaggagtc
     1681 ttagatgcat ctcttgtacg ttgatgagtc tggtacacca tcagactcta ctcttaatca
     1741 ttttgtcctt tctggtatat cagtatttga aaagcaaact cactggatag agcaagagat
     1801 gaatgcaatt gcggctcgat ttaatgctga tgacccgtat gaaagagaat tgcatggttc
     1861 accgatgaga ggtggacgag atggctggaa aaatttttct ttcaatgaca gaaaacaagc
     1921 gatcaaagat tgcttagcac tccttactca aaagcatcgg gatattcgca tatttgcatg
     1981 tgttgtaccc aagggagcct tagatgggaa ggatgtaata acagagtctt tttatcaaat
     2041 atcgtctcgt ttcgacatgt atttaagtcg cttacatcac caaggagcaa cgtctcgagg
     2101 ggtggccatt tttgataaat cgtcgacaga aaaaagtatt caatctcttg cccgtacgtt
     2161 taaagatact ggatatgagt atggaaaact tcgcaacttc tctgaagtac ctttattttt
     2221 ggattccaaa gcgtcaaggc tgattcagct tgctgatctt gtgtcgtact caattttcag
     2281 gaagtttgag ttcaatgatg atgagttcta caaaatcata gagaatcgct ttgactacca
     2341 taatggtaaa gttcacgggc tttacgtagc ccactaatgg aatagttgtg aatacgcact
     2401 atctataagt aagagttcct tgtgaccatc gacgcgaagc ccgctacgcg ggctttttca
     2461 tgcgtagcgt acaacggatg ttatggtaaa tagagcgttt tggtgactcg cacgccacat
     2521 tttcagatgt gatatcatta cgtcctaggg gtgaaaaggg caaaaaaaaa gaacccggaa
     2581 tggttctttt ttttgcctca atcagcgact gcgaatcgct gactgctacc cgaaaacatg
     2641 taccgaagct cacgttgtcg ttacttatag caaagtaatt ttacccctgg ggtgaatcta
     2701 cgtcaacgtg agccggtatt cctttctatc aatatgaagg aataaacgta tggccgaact
     2761 gcttacggcg tttattaaag caccacttat aagccaggtg ctgaccatca ttctgtgtct
     2821 gatctttgta gtacacagtc ttttaaattg acgtaccacc cataaaaaaa gccacttatg
     2881 tggctttttt tatgaatcga gagataggta tctgttgaat atcacagatg atcttcttga
     2941 gatccttttc gttgcgtcgt aatctcttgc tctggaaacg aaaaaaccgc ccagcaaggc
     3001 ggtttttcga aggttctcag agcagcaact ctttgaaccg cggtaactgg cttaagagga
     3061 gcgcagtaac caaatctgtc ctttcagttt agccttaact ggcacataac ttcaagacta
     3121 actcctctaa atcagttacc agtggctgct gccagtggcg cttttgcatg tctttccggg
     3181 ttggactcaa gtcgatagtt accggaacag gcgcagcagt cggactgaac ggggggttcg
     3241 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     3301 agataaacgc ggccataaca gcggaatgac accggtacac cgaaaggcag gaacaggaga
     3361 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc
     3421 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     3481 aacggcttcg ccgtggcctt attgcttctc cgttaagctt cttcttggca tctcccagga
     3541 aatttccgcc ccgcctgtaa gttggtaccg ctcgccgcag ccgaacgacc gagcgcagcg
     3601 agtc
//
