LOCUS       NZ_CP104674             1123 bp    DNA     linear   CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2021CK-00607 plasmid unnamed9.
ACCESSION   NZ_CP104674
VERSION     NZ_CP104674.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17815181
            Assembly: GCF_025398375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:50:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,202
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 81 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 44 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104674.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-00607"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA"
                     /collection_date="2021-01-11"
     gene            complement(<1..188)
                     /locus_tag="N5914_RS26505"
                     /old_locus_tag="N5914_26515"
                     /pseudo=""
     CDS             complement(<1..188)
                     /locus_tag="N5914_RS26505"
                     /old_locus_tag="N5914_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /name="rep protein"
     gene            598..1074
                     /locus_tag="N5914_RS26510"
                     /old_locus_tag="N5914_26520"
     CDS             598..1074
                     /locus_tag="N5914_RS26510"
                     /old_locus_tag="N5914_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          116..201
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="95.0"
                     /evalue="4.39e-26"
     Typing          complement(411..519)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
ORIGIN
        1 acatgcgaga ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag tgagcgagtg
       61 ggcgaggaag cggaagagaa ctggaaacca cattgagcac gtacgcactg atacggggtg
      121 tcggggcgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac ggtattacat
      181 ttgcacatcc tgtcccgatt tctgcggggg ttaacggtga acggacggca aaaaacgtgc
      241 aggagctgtt gaacgtgaaa accacgaaat ctggcaaaaa tcacggctgg cgaggcttgc
      301 agaggagtaa gccagtatac actccgctat cgctacgtga ctgggtcagg gcttcgcccc
      361 gacaccccca aagggcgttg cgttgcgcgc aacacccttg cctagaatag atcttttacg
      421 ccgatttgta agttgttgtt tttttagtgt gttttttgtt tacctgtcgc atccagggtg
      481 acacaacgtt gtcgcatcct ggatgcgaca acacgcaaca aggtgttgca cagatgtgtc
      541 atgatggctt agatttgaac caacgttagt gcatgggatt tttagaggga aaaaatcatg
      601 tttattgatt cagaaaaacg actgaaacaa ctttcagatg aggcaaagaa aaacaccgag
      661 gatctcgaag aagcaaagaa gaattcaagg tttacacagg tatccccaaa aggttgggaa
      721 cgcgttcgag agctgctgaa ggatagccaa ggcatatcag cactgaagct gtactcattt
      781 ttagcggagc atatcgatcc tacgtgtggc gctgtcgttg cggatcagca attcctagct
      841 gaaaaacttg gagttagcag aagcacaatt attcgttggc tcaattactt agaatcaaaa
      901 aatgcattag ttagaatccc cgttgctggt aaggtttgtg cgtatgccct cgatccacat
      961 gaagtctgga agggatacaa cactacgaaa aaccatgcag catttgtcac taaaacactg
     1021 gtcaacaaag acggtgatat tcagcgccga atcatggcca tgttttcaaa ttgagccagc
     1081 ggcaggcgga caatcagggg ttacgtgtta acgttctgac cat
//
